DK3313982T3 - Fremgangsmåder og indretning til konditionering af cellepopulationer til celleterapier - Google Patents

Fremgangsmåder og indretning til konditionering af cellepopulationer til celleterapier Download PDF

Info

Publication number
DK3313982T3
DK3313982T3 DK16815316.1T DK16815316T DK3313982T3 DK 3313982 T3 DK3313982 T3 DK 3313982T3 DK 16815316 T DK16815316 T DK 16815316T DK 3313982 T3 DK3313982 T3 DK 3313982T3
Authority
DK
Denmark
Prior art keywords
cell
methods
conditioning
therapies
cell populations
Prior art date
Application number
DK16815316.1T
Other languages
English (en)
Inventor
Charles S Cox
Brijesh S Gill
Kevin Aroom
Pamela Wenzel
Original Assignee
Univ Texas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Texas filed Critical Univ Texas
Application granted granted Critical
Publication of DK3313982T3 publication Critical patent/DK3313982T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/04—Flat or tray type, drawers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/40—Manifolds; Distribution pieces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/44—Multiple separable units; Modules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/08—Chemical, biochemical or biological means, e.g. plasma jet, co-culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/02—Compounds of the arachidonic acid pathway, e.g. prostaglandins, leukotrienes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2301—Interleukin-1 (IL-1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/24—Interferons [IFN]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/25—Tumour necrosing factors [TNF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Mechanical Engineering (AREA)
  • General Chemical & Material Sciences (AREA)
  • Rheumatology (AREA)
  • Epidemiology (AREA)
  • Virology (AREA)
  • Neurology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Neurosurgery (AREA)
  • Molecular Biology (AREA)
  • Physiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DK16815316.1T 2015-06-23 2016-06-23 Fremgangsmåder og indretning til konditionering af cellepopulationer til celleterapier DK3313982T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562183273P 2015-06-23 2015-06-23
PCT/US2016/039044 WO2016210150A1 (en) 2015-06-23 2016-06-23 Methods and apparatus for conditioning cell populations for cell therapies

Publications (1)

Publication Number Publication Date
DK3313982T3 true DK3313982T3 (da) 2023-12-11

Family

ID=57585755

Family Applications (1)

Application Number Title Priority Date Filing Date
DK16815316.1T DK3313982T3 (da) 2015-06-23 2016-06-23 Fremgangsmåder og indretning til konditionering af cellepopulationer til celleterapier

Country Status (14)

Country Link
US (3) US10669516B2 (da)
EP (1) EP3313982B1 (da)
JP (2) JP6732343B2 (da)
KR (2) KR102558605B1 (da)
CN (2) CN114561277A (da)
AU (4) AU2016281662B2 (da)
BR (1) BR112017028040A2 (da)
CA (2) CA2990231C (da)
DK (1) DK3313982T3 (da)
FI (1) FI3313982T3 (da)
HK (1) HK1254208A1 (da)
IL (3) IL305121A (da)
RU (2) RU2021135194A (da)
WO (1) WO2016210150A1 (da)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2021135194A (ru) 2015-06-23 2022-01-04 Борд Оф Риджентс Оф Дзе Юниверсити Оф Техас Систем Способы и аппараты для подготовки клеточных популяций для клеточной терапии
KR102082745B1 (ko) * 2018-05-08 2020-02-28 주식회사 휴코드 항염증 성분을 분비하는 줄기세포 배양방법 및 그 줄기세포 배양액을 포함하는 항염증 조성물
WO2020027336A1 (ja) * 2018-08-03 2020-02-06 株式会社細胞応用技術研究所 培養上清製剤の製造方法
US20200248124A1 (en) 2019-02-05 2020-08-06 Corning Incorporated Methods of culturing cells on woven cell culture substrates and bioreactors using the same
US11118151B2 (en) 2019-11-05 2021-09-14 Corning Incorporated Fixed bed bioreactor and methods of using the same
EP4065685A1 (en) * 2019-11-27 2022-10-05 Corning Incorporated Stacked fixed bed bioreactor and methods of using the same
EP3981867A1 (en) * 2020-10-06 2022-04-13 Lonza Cologne GmbH Device for providing a cell suspension

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2911508A1 (de) 1978-03-28 1979-10-04 Kuraray Co Fluidbehandlungsvorrichtung
US4661458A (en) 1983-08-31 1987-04-28 Cell Environmental Systems, Ltd. Cell culture system
US5416022A (en) 1988-08-10 1995-05-16 Cellex Biosciences, Inc. Cell culture apparatus
US5240854A (en) * 1989-06-05 1993-08-31 Berry Eric S Continuous high-density cell culture system
US5399493A (en) * 1989-06-15 1995-03-21 The Regents Of The University Of Michigan Methods and compositions for the optimization of human hematopoietic progenitor cell cultures
GB8921458D0 (en) * 1989-09-22 1989-11-08 Univ Strathclyde Cell culture apparatus
WO1992005243A1 (en) * 1990-09-19 1992-04-02 Scott Berry E Continuous high-density cell culture system
JP2585866B2 (ja) * 1990-09-19 1997-02-26 ベリー，エリック・スコット 連続的な高密度の細胞培養システム
RU2164240C2 (ru) * 1990-12-17 2001-03-20 Риджентс оф дзе Юниверсити оф Мичиган Способ культивирования, фибробласт, способ отделения клеток
FI100409B (fi) * 1994-11-28 1997-11-28 Asm Int Menetelmä ja laitteisto ohutkalvojen valmistamiseksi
BE1009306A5 (fr) 1995-04-28 1997-02-04 Baxter Int Bioreacteur.
US5928945A (en) 1996-11-20 1999-07-27 Advanced Tissue Sciences, Inc. Application of shear flow stress to chondrocytes or chondrocyte stem cells to produce cartilage
JP2001508302A (ja) 1997-01-10 2001-06-26 ライフ テクノロジーズ，インコーポレイテッド 胚性幹細胞血清置換
WO2000078920A1 (en) 1999-06-21 2000-12-28 The General Hospital Corporation Methods and devices for cell culturing and organ assist systems
WO2004076608A2 (en) * 2003-02-26 2004-09-10 Georgia Tech Research Corporation Bioreactor and methods for tissue growth and conditioning
US20080057571A1 (en) 2006-04-28 2008-03-06 North Carolina State University Bioreactor device for exposing a cell culture to a fluid shear force imparted by a fluid flow
GB0808373D0 (en) * 2008-05-09 2008-06-18 Synexa Life Sciences Pty Ltd Scalable cell culture bioreactor and cell culture process
WO2010148275A2 (en) * 2009-06-18 2010-12-23 Kiyatec, Llc Bioreactor system
US20120322097A1 (en) * 2011-06-15 2012-12-20 The Charles Stark Draper Laboratory, Inc. Device and method for culturing cells in a biomimetic environment
WO2013086284A1 (en) * 2011-12-09 2013-06-13 University Of Florida Research Foundation, Inc. Flow chambers, methods of using the flow chamber, and methods of making the flow chamber
AU2013277290A1 (en) 2012-06-21 2015-01-22 Neostem Oncology, Llc Bioreactor cartridge and system
US9005550B2 (en) * 2012-10-29 2015-04-14 Corning Incorporated Multi-layered cell culture vessel with manifold grips
RU2021135194A (ru) 2015-06-23 2022-01-04 Борд Оф Риджентс Оф Дзе Юниверсити Оф Техас Систем Способы и аппараты для подготовки клеточных популяций для клеточной терапии

Also Published As

Publication number Publication date
AU2022203657A1 (en) 2022-06-16
AU2016281662B2 (en) 2021-12-02
HK1254208A1 (zh) 2019-07-12
AU2022200689A1 (en) 2022-02-24
CA2990231A1 (en) 2016-12-29
IL256448B (en) 2022-01-01
IL288852A (en) 2022-02-01
US20220010257A1 (en) 2022-01-13
CN107849523A (zh) 2018-03-27
IL305121A (en) 2023-10-01
IL288852B2 (en) 2024-01-01
US11162063B2 (en) 2021-11-02
US20200248128A1 (en) 2020-08-06
US20180187141A1 (en) 2018-07-05
AU2022203657B2 (en) 2024-06-20
AU2022200689B2 (en) 2022-03-17
KR20230114316A (ko) 2023-08-01
CN107849523B (zh) 2021-12-10
US10669516B2 (en) 2020-06-02
IL288852B1 (en) 2023-09-01
EP3313982B1 (en) 2023-09-13
KR20180020256A (ko) 2018-02-27
JP2020156519A (ja) 2020-10-01
JP6732343B2 (ja) 2020-07-29
RU2021135194A (ru) 2022-01-04
WO2016210150A1 (en) 2016-12-29
BR112017028040A2 (pt) 2018-08-28
EP3313982A4 (en) 2019-02-20
IL256448A (en) 2018-02-28
KR102558605B1 (ko) 2023-07-25
RU2761634C2 (ru) 2021-12-13
EP3313982A1 (en) 2018-05-02
CA2990231C (en) 2024-06-04
CA3236202A1 (en) 2016-12-29
CN114561277A (zh) 2022-05-31
RU2018102213A3 (da) 2020-05-18
FI3313982T3 (fi) 2023-12-12
AU2024205686A1 (en) 2024-08-29
JP2018518188A (ja) 2018-07-12
AU2016281662A1 (en) 2018-01-18
JP7074372B2 (ja) 2022-05-24
RU2018102213A (ru) 2019-07-24

Similar Documents

Publication Publication Date Title
DK3259386T3 (da) Systemer og fremgangsmåder til udførelse af immunoassays
DK3215601T3 (da) Fremgangsmåder til transduktion og celleforarbejdning
DK3122870T3 (da) Fremgangsmåder og genetiske systemer til celleteknik
DK3071704T4 (da) System og fremgangsmåde til sortering af sperm
DK3280441T3 (da) Anti-sortilin-antistoffer og fremgangsmåder til anvendelse deraf
DK3227336T3 (da) Anti-CD79b-antistoffer og fremgangsmåder til anvendelse
DK3132247T3 (da) Fremgangsmåder, kits og apparat til udvidelse af en cellepopulation
LU92752B1 (en) Cell culture apparatus and culture methods using same
DK3213065T3 (da) System og fremgangsmåder til todimensionel rplc-sfc-kromatografi
DK3129912T3 (da) Fremgangsmåde og system til sikring af data
DK3220676T3 (da) Fremgangsmåde og apparat til afsendelse af informationer
DK3174976T3 (da) Fremgangsmåder og systemer til forarbejdning af partikler
DK3113883T3 (da) Cellesorteringssystem der anvender mikrofremstillede komponenter
DK3368663T3 (da) Virusfri cellelinjer og fremgangsmåder til opnåelse deraf
DK3237578T3 (da) Fremgangsmåde og anordning til reduktion af tunge aromatiske polycikliske forbindelser i hydrokrakningsenheder
DK3140898T3 (da) Fremgangsmåde og system til overvågning af distributionssystemer
DK3539469T3 (da) System og fremgangsmåde til overvågning af bækkenbundsmusklerne
DK3319612T3 (da) Oxysteroler og fremgansmåder til anvendelse derfor
DK3240892T5 (da) Rpe-cellepopulationer og fremgangsmåder til dannelse deraf
DK3175588T3 (da) Systemer og fremgangsmåder til beskedredigering
DK3313982T3 (da) Fremgangsmåder og indretning til konditionering af cellepopulationer til celleterapier
HK1232324A1 (zh) 用於樣品完整性驗證的系統、裝置和方法
DK3101760T3 (da) Opladningsfremgangsmåde og system
EP3709001C0 (en) SYSTEMS AND METHODS FOR DETERMINING THE OSMOLARITY OF SAMPLES
DK2924093T3 (da) Multicarboxylat-sammensætninger og fremgangsmåde til fremstilling af disse