PL3216867T3 - Sposób modyfikowania sekwencji genomu w celu wprowadzania swoistej mutacji do docelowej sekwencji DNA poprzez reakcję usuwania zasady i stosowany w nim kompleks cząsteczkowy - Google Patents

Sposób modyfikowania sekwencji genomu w celu wprowadzania swoistej mutacji do docelowej sekwencji DNA poprzez reakcję usuwania zasady i stosowany w nim kompleks cząsteczkowy

Info

Publication number
PL3216867T3
PL3216867T3 PL15857325T PL15857325T PL3216867T3 PL 3216867 T3 PL3216867 T3 PL 3216867T3 PL 15857325 T PL15857325 T PL 15857325T PL 15857325 T PL15857325 T PL 15857325T PL 3216867 T3 PL3216867 T3 PL 3216867T3
Authority
PL
Poland
Prior art keywords
base
molecular complex
specific mutation
complex used
removal reaction
Prior art date
Application number
PL15857325T
Other languages
English (en)
Inventor
Keiji Nishida
Akihiko Kondo
Original Assignee
National University Corporation Kobe University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National University Corporation Kobe University filed Critical National University Corporation Kobe University
Publication of PL3216867T3 publication Critical patent/PL3216867T3/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2497—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing N- glycosyl compounds (3.2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/02—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2) hydrolysing N-glycosyl compounds (3.2.2)
    • C12Y302/02027—Uracil-DNA glycosylase (3.2.2.27)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
PL15857325T 2014-11-04 2015-11-02 Sposób modyfikowania sekwencji genomu w celu wprowadzania swoistej mutacji do docelowej sekwencji DNA poprzez reakcję usuwania zasady i stosowany w nim kompleks cząsteczkowy PL3216867T3 (pl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2014224745 2014-11-04
PCT/JP2015/080958 WO2016072399A1 (ja) 2014-11-04 2015-11-02 脱塩基反応により標的化したｄｎａ配列に特異的に変異を導入する、ゲノム配列の改変方法、並びにそれに用いる分子複合体
EP15857325.3A EP3216867B1 (en) 2014-11-04 2015-11-02 Method for modifying genome sequence to introduce specific mutation to targeted dna sequence by base-removal reaction, and molecular complex used therein

Publications (1)

Publication Number Publication Date
PL3216867T3 true PL3216867T3 (pl) 2020-11-02

Family

ID=55909124

Family Applications (1)

Application Number Title Priority Date Filing Date
PL15857325T PL3216867T3 (pl) 2014-11-04 2015-11-02 Sposób modyfikowania sekwencji genomu w celu wprowadzania swoistej mutacji do docelowej sekwencji DNA poprzez reakcję usuwania zasady i stosowany w nim kompleks cząsteczkowy

Country Status (8)

Country Link
US (2) US10920215B2 (pl)
EP (2) EP3739047A1 (pl)
JP (1) JP6153180B2 (pl)
DK (1) DK3216867T3 (pl)
ES (1) ES2800168T3 (pl)
PL (1) PL3216867T3 (pl)
PT (1) PT3216867T (pl)
WO (1) WO2016072399A1 (pl)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3613852A3 (en) 2011-07-22 2020-04-22 President and Fellows of Harvard College Evaluation and improvement of nuclease cleavage specificity
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9737604B2 (en) 2013-09-06 2017-08-22 President And Fellows Of Harvard College Use of cationic lipids to deliver CAS9
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
US20150166982A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting pi3k point mutations
EP3613854A1 (en) 2014-03-05 2020-02-26 National University Corporation Kobe University Genomic sequence modification method for specifically converting nucleic acid bases of targeted dna sequence, and molecular complex for use in same
US10077453B2 (en) 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
ES2938623T3 (es) 2015-09-09 2023-04-13 Univ Kobe Nat Univ Corp Método para convertir una secuencia del genoma de una bacteria gram-positiva mediante una conversión específica de una base de ácido nucleico de una secuencia de ADN seleccionada como diana y el complejo molecular utilizado en el mismo
IL294014B1 (en) 2015-10-23 2024-03-01 Harvard College Nucleobase editors and their uses
KR102061438B1 (ko) 2015-11-27 2019-12-31 고쿠리츠다이가쿠호진 고베다이가쿠 표적화 dna 서열 중의 핵산 염기가 특이적으로 전환되어 있는 단자엽식물 게놈 서열을 전환시키는 방법, 및 그것에 사용되는 분자 복합체
EP3447139B1 (en) * 2016-04-21 2022-06-08 National University Corporation Kobe University Method for increasing mutation introduction efficiency in genome sequence modification technique, and molecular complex to be used therefor
AU2017306676B2 (en) 2016-08-03 2024-02-22 President And Fellows Of Harvard College Adenosine nucleobase editors and uses thereof
CA3033327A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
WO2018035503A1 (en) * 2016-08-18 2018-02-22 The Regents Of The University Of California Crispr-cas genome engineering via a modular aav delivery system
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
CN110214180A (zh) 2016-10-14 2019-09-06 哈佛大学的校长及成员们 核碱基编辑器的aav递送
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
US11898179B2 (en) 2017-03-09 2024-02-13 President And Fellows Of Harvard College Suppression of pain by gene editing
JP2020510439A (ja) * 2017-03-10 2020-04-09 プレジデント アンド フェローズ オブ ハーバード カレッジ シトシンからグアニンへの塩基編集因子
CN110446782B (zh) * 2017-03-22 2024-03-01 国立大学法人神户大学 一种改变细胞所具有的dna的靶向部位的方法以及用于该方法的复合体
KR20190130613A (ko) 2017-03-23 2019-11-22 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
WO2019023680A1 (en) 2017-07-28 2019-01-31 President And Fellows Of Harvard College METHODS AND COMPOSITIONS FOR EVOLUTION OF BASIC EDITORS USING PHAGE-ASSISTED CONTINUOUS EVOLUTION (PACE)
US11319532B2 (en) 2017-08-30 2022-05-03 President And Fellows Of Harvard College High efficiency base editors comprising Gam
US11795443B2 (en) 2017-10-16 2023-10-24 The Broad Institute, Inc. Uses of adenosine base editors
CA3082922C (en) 2017-11-22 2024-02-13 National University Corporation Kobe University Complex for genome editing having stability and few side-effects, and nucleic acid coding same
EP3765478A4 (en) * 2018-03-15 2022-03-16 Massachusetts Institute of Technology METHODS FOR QUANTIFICATION OF RNA AND DNA VARIANTS BY SEQUENCING USING PHOSPHOROTHIOATES
WO2019189147A1 (ja) 2018-03-26 2019-10-03 国立大学法人神戸大学 細胞の有する二本鎖ｄｎａの標的部位を改変する方法
KR20210041008A (ko) * 2018-08-03 2021-04-14 빔 테라퓨틱스, 인크. 핵산 표적 서열을 변형시키기 위한 다중-이펙터 핵염기 편집기 및 이를 이용하는 방법
CN112585274A (zh) * 2018-08-21 2021-03-30 先锋国际良种公司 用于在稻植物中改变成熟的组合物和方法
EP3942040A1 (en) 2019-03-19 2022-01-26 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
WO2020229241A1 (en) 2019-05-10 2020-11-19 Basf Se Regulatory nucleic acid molecules for enhancing gene expression in plants
WO2021046155A1 (en) 2019-09-03 2021-03-11 Voyager Therapeutics, Inc. Vectorized editing of nucleic acids to correct overt mutations
BR112022004545A2 (pt) 2019-09-12 2022-05-31 Basf Se Métodos para aumentar a expressão derivada de um promotor vegetal e produzir uma planta, construção de expressão recombinante, vetor de expressão, célula ou planta transgênica, cultura de células transgênicas e usos
WO2021069387A1 (en) 2019-10-07 2021-04-15 Basf Se Regulatory nucleic acid molecules for enhancing gene expression in plants
AU2020396138A1 (en) 2019-12-03 2022-06-16 Basf Se Regulatory nucleic acid molecules for enhancing gene expression in plants
US20230040261A1 (en) * 2020-03-11 2023-02-09 North Carolina State University Compositions, methods, and systems for genome editing technology
MX2022014008A (es) 2020-05-08 2023-02-09 Broad Inst Inc Métodos y composiciones para la edición simultánea de ambas cadenas de una secuencia de nucleótidos de doble cadena objetivo.
WO2022158561A1 (ja) * 2021-01-22 2022-07-28 国立大学法人東京大学 植物ゲノムの編集方法
WO2022181796A1 (ja) 2021-02-26 2022-09-01 国立大学法人神戸大学 植物における半合理的なゲノム進化工学手法
WO2024048599A1 (ja) * 2022-08-31 2024-03-07 国立大学法人東京農工大学 タンパク質－核酸複合体、これを用いた物質の検出キット及び検出方法
WO2024083579A1 (en) 2022-10-20 2024-04-25 Basf Se Regulatory nucleic acid molecules for enhancing gene expression in plants

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9600384D0 (en) * 1996-01-09 1996-03-13 Nyfotek As Dna glycosylases
AU9036198A (en) * 1997-08-26 1999-03-16 Ariad Gene Therapeutics, Inc. Fusion proteins comprising a dimerization, trimerization or tetramerization domain and an additional heterologous transcription activation, transcription repression, dna binding or ligand binding domain
AU2003251286B2 (en) 2002-01-23 2007-08-16 The University Of Utah Research Foundation Targeted chromosomal mutagenesis using zinc finger nucleases
RU2531343C2 (ru) 2007-03-02 2014-10-20 ДюПон Ньютришн Байосайенсиз АпС, Способ генерирования заквасочной культуры, заквасочная культура и способ ферментации с ее использованием
WO2011072246A2 (en) 2009-12-10 2011-06-16 Regents Of The University Of Minnesota Tal effector-mediated dna modification
JP2013128413A (ja) 2010-03-11 2013-07-04 Kyushu Univ Ｐｐｒモチーフを利用したｒｎａ結合性蛋白質の改変方法
US9040239B2 (en) * 2012-03-15 2015-05-26 New England Biolabs, Inc. Composition and methods of oxygenation of nucleic acids containing 5-methylpyrimidine
WO2014127287A1 (en) * 2013-02-14 2014-08-21 Massachusetts Institute Of Technology Method for in vivo tergated mutagenesis
JP2016514480A (ja) * 2013-04-05 2016-05-23 ダウ アグロサイエンシィズ エルエルシー 植物のゲノム内に外因性配列を組み込むための方法および組成物
JP2014224745A (ja) 2013-05-16 2014-12-04 株式会社ミツトヨ 原点信号発生装置及び原点信号発生システム
US20150037790A1 (en) * 2013-07-05 2015-02-05 The University Of Southampton Cytosine variant detection
US20150166982A1 (en) * 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting pi3k point mutations
EP3613854A1 (en) 2014-03-05 2020-02-26 National University Corporation Kobe University Genomic sequence modification method for specifically converting nucleic acid bases of targeted dna sequence, and molecular complex for use in same

Also Published As

Publication number Publication date
PT3216867T (pt) 2020-07-16
EP3739047A1 (en) 2020-11-18
JP6153180B2 (ja) 2017-06-28
JPWO2016072399A1 (ja) 2017-04-27
ES2800168T3 (es) 2020-12-28
WO2016072399A1 (ja) 2016-05-12
US20210171935A1 (en) 2021-06-10
EP3216867A4 (en) 2018-04-04
EP3216867B1 (en) 2020-04-15
US20170321210A1 (en) 2017-11-09
EP3216867A1 (en) 2017-09-13
US10920215B2 (en) 2021-02-16
DK3216867T3 (da) 2020-06-22

Similar Documents

Publication Publication Date Title
PT3216867T (pt) Método para modificar a sequência de genoma para introduzir mutação específica a sequência de adn alvo por reação de remoção de bases, e complexo molecular nele utilizado
HK1253544A1 (zh) 通過特異性地轉化靶dna序列的核碱基轉化革蘭氏陽性細菌的基因組序列的方法，以及用於該方法的分子複合物
HK1253540A1 (zh) 用於特異性轉變靶向dna序列的核酸碱基的基因組序列的修飾方法、及其使用的分子複合體
HK1256888A1 (zh) 用於特異性轉換靶向dna序列的核酸碱基的單子葉植物的基因組序列的轉換方法、及其中使用的分子複合體
SG11201609211VA (en) Genomic sequence modification method for specifically converting nucleic acid bases of targeted dna sequence, and molecular complex for use in same
IL252556A0 (en) Nucleic acid constructs for genome editing
HK1252166A1 (zh) 從環狀rna產生環狀dna的方法
SG11201608503UA (en) Methods and compositions for modifying genomic dna
DK3604544T3 (en) Improved methods for processing dna substrates
PL3155129T3 (pl) Sposób zwiększania wytwarzania RNA
EP3122759A4 (en) Chemical methods for producing tagged nucleotides
LT3129487T (lt) Patobulinti nukleorūgščių konstruktai, skirti eukariotinio geno raiškai
DK3359671T3 (da) Dna-vektorer, transposoner og transposaser til eukaryot genommodifikation
ZA201801947B (en) Compounds as dna probes, methods and applications thereof
SI3094731T1 (sl) S saharidom spremenjene molekule nukleinske kisline
IL251701A0 (en) Oligonucleotides for editing genomic DNA
ZA201500638B (en) Process for designing diverged, codon-optimized large repeated dna sequences
SG11201606620UA (en) Method for recovering two or more genes, or gene products, encoding an immunoreceptor
PL3605100T3 (pl) Sposób katalizowania reakcji katalizowanej enzymem
GB201419167D0 (en) Molecular and bioinformatics methods for direct sequencing
ZA201707935B (en) Herbicide-tolerant maize plant dbn9858, and nucleotide sequence and method for detecting same
SG11201704744QA (en) Methods and kits for purifying plasmid dna
EP3238820A4 (en) Method for removing catalyst used in reaction
EP3231866A4 (en) Nucleic acid complex, method for forming nucleic acid hybridization, pharmaceutical composition, nucleic acid probe, and complementary-strand nucleic acid complex
EP3259355A4 (en) Methods related to dna sequencing