SG11201707515SA - Spatially distinguished, multiplex nucleic acid analysis of biological specimens - Google Patents

Spatially distinguished, multiplex nucleic acid analysis of biological specimens

Info

Publication number
SG11201707515SA
SG11201707515SA SG11201707515SA SG11201707515SA SG11201707515SA SG 11201707515S A SG11201707515S A SG 11201707515SA SG 11201707515S A SG11201707515S A SG 11201707515SA SG 11201707515S A SG11201707515S A SG 11201707515SA SG 11201707515S A SG11201707515S A SG 11201707515SA
Authority
SG
Singapore
Prior art keywords
nucleic acid
acid analysis
biological specimens
multiplex nucleic
spatially distinguished
Prior art date
Application number
SG11201707515SA
Other languages
English (en)
Inventor
Jonas Frisén
Patrik Ståhl
Joakim Lundeberg
Gordon M Cann
Leila Bazargan
Alex Aravanis
Original Assignee
Spatial Transcriptomics Ab
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=55963288&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=SG11201707515S(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Spatial Transcriptomics Ab, Illumina Inc filed Critical Spatial Transcriptomics Ab
Publication of SG11201707515SA publication Critical patent/SG11201707515SA/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1065Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6841In situ hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877Flow chambers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/16Surface properties and coatings
    • B01L2300/161Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B01L2300/163Biocompatibility
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/505Containers for the purpose of retaining a material to be analysed, e.g. test tubes flexible containers not provided for above
    • B01L3/5055Hinged, e.g. opposable surfaces
SG11201707515SA 2015-04-10 2016-04-04 Spatially distinguished, multiplex nucleic acid analysis of biological specimens SG11201707515SA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562145874P 2015-04-10 2015-04-10
PCT/EP2016/057355 WO2016162309A1 (fr) 2015-04-10 2016-04-04 Analyse de plusieurs acides nucléiques spatialement différenciés de spécimens biologiques

Publications (1)

Publication Number Publication Date
SG11201707515SA true SG11201707515SA (en) 2017-10-30

Family

ID=55963288

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201707515SA SG11201707515SA (en) 2015-04-10 2016-04-04 Spatially distinguished, multiplex nucleic acid analysis of biological specimens

Country Status (11)

Country Link
US (10) US10774374B2 (fr)
EP (8) EP3530752B1 (fr)
JP (1) JP6828007B2 (fr)
CN (2) CN107532207B (fr)
CA (1) CA2982146A1 (fr)
DK (2) DK3530752T3 (fr)
ES (3) ES2935860T3 (fr)
FI (1) FI3901281T3 (fr)
HK (1) HK1250245A1 (fr)
SG (1) SG11201707515SA (fr)
WO (1) WO2016162309A1 (fr)

Families Citing this family (188)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
WO2011127099A1 (fr) 2010-04-05 2011-10-13 Prognosys Biosciences, Inc. Tests biologiques à codage spatial
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US11021737B2 (en) 2011-12-22 2021-06-01 President And Fellows Of Harvard College Compositions and methods for analyte detection
WO2013184754A2 (fr) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Séquençage spatial d'acides nucléiques à l'aide de sondes d'origami d'adn
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014210225A1 (fr) 2013-06-25 2014-12-31 Prognosys Biosciences, Inc. Procédés et systèmes pour déterminer des motifs spatiales de cibles biologiques dans un échantillon
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
MX2016016902A (es) 2014-06-26 2017-03-27 10X Genomics Inc Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas.
CN112782140A (zh) 2014-12-03 2021-05-11 伊索普莱西斯公司 细胞分泌特征的分析和筛选
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
EP3530752B1 (fr) 2015-04-10 2021-03-24 Spatial Transcriptomics AB Analyse de plusieurs acides nucléiques spatialement différenciés de spécimens biologiques
US20180057873A1 (en) * 2015-04-17 2018-03-01 Centrillion Technology Holdings Corporation Methods for performing spatial profiling of biological materials
US10672505B2 (en) * 2015-06-03 2020-06-02 General Electric Company Biological data annotation and visualization
WO2017015099A1 (fr) 2015-07-17 2017-01-26 Nanostring Technologies, Inc. Quantification simultanée de l'expression génique dans une région définie par l'utilisateur d'un tissu en coupe transversale
JP6882282B2 (ja) * 2015-11-03 2021-06-02 プレジデント アンド フェローズ オブ ハーバード カレッジ 三次元核酸含有マトリックスの立体撮像のための方法と装置
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
DK3882357T3 (da) 2015-12-04 2022-08-29 10X Genomics Inc Fremgangsmåder og sammensætninger til analyse af nukleinsyrer
CN109415761B (zh) 2016-04-25 2022-09-20 哈佛学院董事及会员团体 用于原位分子检测的杂交链反应方法
WO2017205691A1 (fr) 2016-05-26 2017-11-30 Cellular Research, Inc. Procédés de réglage de comptage d'étiquettes moléculaires
EP3472359B1 (fr) 2016-06-21 2022-03-16 10X Genomics, Inc. Séquençage d'acide nucléique
CN109952612B (zh) * 2016-11-08 2023-12-01 贝克顿迪金森公司 用于表达谱分类的方法
JP7228510B2 (ja) 2016-11-08 2023-02-24 ベクトン・ディキンソン・アンド・カンパニー 細胞標識分類の方法
DK3538891T3 (da) 2016-11-11 2022-03-28 Isoplexis Corp Sammensætninger og fremgangsmåder til samtidig genomisk, transkriptomisk og proteomisk analyse af enkeltceller
GB201619458D0 (en) 2016-11-17 2017-01-04 Spatial Transcriptomics Ab Method for spatial tagging and analysing nucleic acids in a biological specimen
CN110226084A (zh) 2016-11-22 2019-09-10 伊索普莱克西斯公司 用于细胞捕获的系统、装置和方法,以及其制造方法
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4029939B1 (fr) 2017-01-30 2023-06-28 10X Genomics, Inc. Procédés et systèmes de codage à barres de cellules individuelles sur la base de gouttelettes
CN111051526A (zh) * 2017-05-23 2020-04-21 生捷科技控股公司 用于实施生物分子空间分布分析的方法
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
CN111263819A (zh) 2017-10-06 2020-06-09 卡特阿纳公司 Rna模板化连接
EP3696275A4 (fr) * 2017-10-11 2021-05-26 MGI Tech Co., Ltd. Procédé d'amélioration du chargement et de la stabilité d'acide nucléique sur un support solide
EP3700672B1 (fr) 2017-10-27 2022-12-28 10X Genomics, Inc. Procédés de préparation et d'analyse d'échantillons
EP3625361A1 (fr) 2017-11-15 2020-03-25 10X Genomics, Inc. Perles de gel fonctionnalisées
CN110079592B (zh) * 2018-01-26 2021-02-12 厦门艾德生物医药科技股份有限公司 用于检测基因突变和已知、未知基因融合类型的高通量测序靶向捕获目标区域的探针和方法
WO2019157529A1 (fr) 2018-02-12 2019-08-15 10X Genomics, Inc. Procédés de caractérisation d'analytes multiples à partir de cellules individuelles ou de populations cellulaires
JP7372927B6 (ja) 2018-02-12 2023-11-27 ナノストリング テクノロジーズ,インコーポレイティド 遺伝子およびタンパク質の発現を検出する生体分子プローブおよびその検出方法
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
EP3788146A4 (fr) * 2018-05-02 2022-06-01 The General Hospital Corporation Évaluation d'abondance de macromolécules spatiales à haute résolution
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
SG11202102029TA (en) 2018-08-28 2021-03-30 10X Genomics Inc Methods for generating spatially barcoded arrays
US20210317524A1 (en) 2018-08-28 2021-10-14 10X Genomics, Inc. Resolving spatial arrays
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
CN112840023B (zh) * 2018-10-25 2024-03-01 Illumina公司 使用索引和条形码识别阵列上的配体的方法和组合物
US11208648B2 (en) 2018-11-16 2021-12-28 International Business Machines Corporation Determining position and transcriptomes of biological cells
CA3120107A1 (fr) * 2018-11-30 2020-06-04 Geneinfosec, Inc. Procede de generation d'oligonucleotides aleatoires et de determination de leur sequence
EP3894587A1 (fr) 2018-12-10 2021-10-20 10X Genomics, Inc. Résolution de réseaux spatiaux par déconvolution basée sur la proximité
GB201820341D0 (en) 2018-12-13 2019-01-30 10X Genomics Inc Method for transposase-mediated spatial tagging and analysing genomic DNA in a biological specimen
GB201820300D0 (en) 2018-12-13 2019-01-30 10X Genomics Inc Method for spatial tagging and analysing genomic DNA in a biological specimen
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
WO2020168013A1 (fr) 2019-02-12 2020-08-20 10X Genomics, Inc. Procédés de traitement de molécules d'acides nucléiques
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
EP3931354A1 (fr) 2019-02-28 2022-01-05 10X Genomics, Inc. Profilage d'analytes biologiques avec des réseaux d'oligonucléotides à codes-barres spatiaux
WO2020180670A1 (fr) * 2019-03-01 2020-09-10 Revere Biosensors, Llc Systèmes et procédés de séparation de réseaux décodés
US11920183B2 (en) 2019-03-11 2024-03-05 10X Genomics, Inc. Systems and methods for processing optically tagged beads
EP3938538A1 (fr) 2019-03-15 2022-01-19 10X Genomics, Inc. Méthodes d'utilisation de matrices spatiales pour le séquençage d'organismes unicellulaires
WO2020198071A1 (fr) 2019-03-22 2020-10-01 10X Genomics, Inc. Analyse spatiale tridimensionnelle
US20230175047A1 (en) * 2019-05-15 2023-06-08 Bgi Shenzhen Array and method for detecting spatial information of nucleic acids
WO2020243579A1 (fr) 2019-05-30 2020-12-03 10X Genomics, Inc. Procédés de détection de l'hétérogénéité spatiale d'un échantillon biologique
AU2020282024A1 (en) 2019-05-31 2021-11-11 10X Genomics, Inc. Method of detecting target nucleic acid molecules
CN112654719A (zh) * 2019-05-31 2021-04-13 伊鲁米纳公司 使用流动池进行信息存储和检索的系统和方法
EP3986907A1 (fr) 2019-06-21 2022-04-27 Thermo Fisher Scientific Baltics UAB Nucléotides triphosphates attachés à des oligonucléotides pouvant servir au marquage d'acides nucléiques pour la préparation de bibliothèques de séquençage de nouvelle génération
EP4038546A1 (fr) 2019-10-01 2022-08-10 10X Genomics, Inc. Systèmes et procédés d'identification de motifs morphologiques dans des échantillons de tissu
US20210130881A1 (en) 2019-11-06 2021-05-06 10X Genomics, Inc. Imaging system hardware
EP4055159A4 (fr) * 2019-11-06 2024-04-17 Adaptive Biotechnologies Corp Brins synthétiques pour séquençage d'acide nucléique et procédés et systèmes associés
EP4025711A2 (fr) 2019-11-08 2022-07-13 10X Genomics, Inc. Amélioration de la spécificité de la liaison d'un analyte
WO2021091611A1 (fr) 2019-11-08 2021-05-14 10X Genomics, Inc. Agents de capture d'analytes marqués spatialement pour le multiplexage d'analytes
US20230002812A1 (en) 2019-11-13 2023-01-05 10X Genomics, Inc. Generating capture probes for spatial analysis
CN115004260A (zh) 2019-11-18 2022-09-02 10X基因组学有限公司 用于组织分类的系统和方法
US20210155982A1 (en) 2019-11-21 2021-05-27 10X Genomics, Inc. Pipeline for spatial analysis of analytes
CA3158891A1 (fr) 2019-11-22 2021-05-27 Neil Ira WEISENFELD Systemes et procedes d'analyse spatiale d'analytes a l'aide d'un alignement de reperes
AU2020405034A1 (en) * 2019-12-19 2021-09-30 Illumina, Inc. Designing probes for depleting abundant transcripts
EP3891300B1 (fr) 2019-12-23 2023-03-29 10X Genomics, Inc. Procédés d'analyse spatiale utilisant une ligature à matrice d'arn
CN115715329A (zh) 2020-01-10 2023-02-24 10X基因组学有限公司 确定生物样品中靶核酸位置的方法
US20210214785A1 (en) * 2020-01-13 2021-07-15 Spatial Transcriptomics Ab Methods of decreasing background on a spatial array
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210222253A1 (en) * 2020-01-21 2021-07-22 10X Genomics, Inc. Identification of biomarkers of glioblastoma and methods of using the same
US11821035B1 (en) * 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US20230047782A1 (en) 2020-02-07 2023-02-16 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
WO2021168278A1 (fr) 2020-02-20 2021-08-26 10X Genomics, Inc. Procédés pour combiner les synthèses d'adnc des premier et de deuxième brins pour l'analyse spatiale
AU2021225020A1 (en) 2020-02-21 2022-08-18 10X Genomics, Inc. Methods and compositions for integrated in situ spatial assay
AU2021224760A1 (en) 2020-02-21 2022-09-15 10X Genomics, Inc. Capturing genetic targets using a hybridization approach
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
WO2021173666A1 (fr) * 2020-02-26 2021-09-02 Illumina, Inc. Kits pour le génotypage
MX2021015806A (es) * 2020-02-26 2022-04-27 Illumina Inc Kits para genotipificacion.
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
WO2021216708A1 (fr) 2020-04-22 2021-10-28 10X Genomics, Inc. Procédés d'analyse spatiale utilisant un appauvrissement d'arn ciblée
EP4146819A1 (fr) 2020-05-04 2023-03-15 10X Genomics, Inc. Modes de transfert transcriptomique spatial
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
US20230194469A1 (en) 2020-05-19 2023-06-22 10X Genomics, Inc. Electrophoresis cassettes and instrumentation
WO2021237087A1 (fr) 2020-05-22 2021-11-25 10X Genomics, Inc. Analyse spatiale pour détecter des variants de séquence
WO2021237056A1 (fr) 2020-05-22 2021-11-25 10X Genomics, Inc. Analyse d'intégrité d'arn dans un échantillon biologique
WO2021236929A1 (fr) 2020-05-22 2021-11-25 10X Genomics, Inc. Mesure spatio-temporelle simultanée de l'expression génique et de l'activité cellulaire
WO2021242834A1 (fr) 2020-05-26 2021-12-02 10X Genomics, Inc. Procédé de réinitialisation d'un réseau
AU2021283174A1 (en) 2020-06-02 2023-01-05 10X Genomics, Inc. Nucleic acid library methods
WO2021247568A1 (fr) 2020-06-02 2021-12-09 10X Genomics, Inc. Trancriptomique spatiale pour les récepteurs d'antigènes
EP4162074B1 (fr) 2020-06-08 2024-04-24 10X Genomics, Inc. Méthodes de détermination de marge chirurgicale et méthodes d'utilisation associées
US20230279474A1 (en) * 2020-06-10 2023-09-07 10X Genomics, Inc. Methods for spatial analysis using blocker oligonucleotides
WO2021252591A1 (fr) 2020-06-10 2021-12-16 10X Genomics, Inc. Procédés de détermination d'un emplacement d'un analyte dans un échantillon biologique
CN116034166A (zh) 2020-06-25 2023-04-28 10X基因组学有限公司 Dna甲基化的空间分析
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
WO2022008649A1 (fr) * 2020-07-08 2022-01-13 F. Hoffmann-La Roche Ag Analyse spatiale de multiples cibles dans des échantillons de tissu
US20240043913A1 (en) * 2020-07-17 2024-02-08 The Regents Of The University Of Michigan Materials and methods for localized detection of nucleic acids in a tissue sample
CN116323968A (zh) 2020-07-31 2023-06-23 10X基因组学有限公司 用于空间分析的去交联化合物和使用方法
WO2022032194A1 (fr) 2020-08-06 2022-02-10 Singular Genomics Systems, Inc. Méthodes pour la transcriptomique et la protéomique in situ
WO2022032195A2 (fr) 2020-08-06 2022-02-10 Singular Genomics Systems, Inc. Séquençage spatial
EP4200441A1 (fr) 2020-09-15 2023-06-28 10X Genomics, Inc. Procédés de libération d'une sonde de capture étendue à partir d'un substrat et leurs utilisations
AU2021345133A1 (en) 2020-09-16 2023-03-30 10X Genomics, Inc. Methods of determining the location of an analyte in a biological sample using a plurality of wells
US20240033743A1 (en) 2020-09-18 2024-02-01 10X Genomics, Inc. Sample handling apparatus and fluid delivery methods
CN116194590A (zh) 2020-09-18 2023-05-30 10X基因组学有限公司 样品处理设备和图像配准方法
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
WO2022081643A2 (fr) 2020-10-13 2022-04-21 10X Genomics, Inc. Compositions et procédés pour générer des molécules de liaison à un antigène recombinant à partir de cellules uniques
WO2022087273A1 (fr) 2020-10-22 2022-04-28 10X Genomics, Inc. Procédés d'analyse spatiale utilisant l'amplification par cercle roulant
EP4222283A1 (fr) 2020-11-06 2023-08-09 10X Genomics, Inc. Compositions et procédés de liaison d'un analyte à une sonde de capture
AU2021385065A1 (en) 2020-11-18 2023-06-15 10X Genomics, Inc. Methods and compositions for analyzing immune infiltration in cancer stroma to predict clinical outcome
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4012046A1 (fr) 2020-12-11 2022-06-15 10X Genomics, Inc. Procédés et compositions pour l'analyse multimodale in situ
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US20240068017A1 (en) 2020-12-30 2024-02-29 10X Genomics, Inc. Methods for analyte capture determination
WO2022147296A1 (fr) 2020-12-30 2022-07-07 10X Genomics, Inc. Clivage de sondes de capture pour analyse spatiale
WO2022147140A1 (fr) 2020-12-30 2022-07-07 10X Genomics, Inc. Génération de matrice moléculaire au moyen d'une résine photosensible
US20240093290A1 (en) 2021-01-29 2024-03-21 10X Genomics, Inc. Method for transposase mediated spatial tagging and analyzing genomic dna in a biological sample
WO2022182682A1 (fr) 2021-02-23 2022-09-01 10X Genomics, Inc. Analyse à base de sonde d'acides nucléiques et de protéines
EP4301870A1 (fr) 2021-03-18 2024-01-10 10X Genomics, Inc. Capture multiplex de gène et expression de protéines à partir d'un échantillon biologique
EP4319792A1 (fr) 2021-04-05 2024-02-14 10X Genomics, Inc. Composition de ligase recombinante et ses utilisations
EP4305196A1 (fr) 2021-04-14 2024-01-17 10X Genomics, Inc. Procédés de mesure d'une mauvaise localisation d'un analyte
WO2022226057A1 (fr) 2021-04-20 2022-10-27 10X Genomics, Inc. Procédés d'évaluation de la qualité d'un échantillon avant une analyse spatiale à l'aide d'une ligature modélisée
EP4320271A1 (fr) 2021-05-06 2024-02-14 10X Genomics, Inc. Procédés pour augmenter la résolution d'une analyse spatiale
WO2022256503A1 (fr) 2021-06-03 2022-12-08 10X Genomics, Inc. Procédés, compositions, kits et systèmes pour améliorer la capture d'analytes pour une analyse spatiale
US20220403462A1 (en) 2021-06-18 2022-12-22 Miltenyi Biotec B.V. & Co. KG Method of spatial sequencing of genes from tissue using padlocks with gaps on substrate
WO2022271820A1 (fr) 2021-06-22 2022-12-29 10X Genomics, Inc. Détection spatiale de sars-cov-2 à l'aide d'une ligature à matrice
US20230026886A1 (en) 2021-07-13 2023-01-26 10X Genomics, Inc. Methods for preparing polymerized matrix with controllable thickness
WO2023287765A1 (fr) 2021-07-13 2023-01-19 10X Genomics, Inc. Procédés d'analyse spatiale utilisant un silençage de sonde ciblée
WO2023018799A1 (fr) 2021-08-12 2023-02-16 10X Genomics, Inc. Procédés, compositions et systèmes pour identifier des molécules de liaison à l'antigène
EP4196605A1 (fr) 2021-09-01 2023-06-21 10X Genomics, Inc. Procédés, compositions et kits pour bloquer une sonde de capture sur un réseau spatial
WO2023044071A1 (fr) 2021-09-17 2023-03-23 10X Genomics, Inc. Systèmes et procédés de recalage ou d'alignement d'images
EP4155416B1 (fr) 2021-09-23 2024-01-10 Miltenyi Biotec B.V. & Co. KG Procédé d'obtention d'informations spatiales et de séquençage d'arn-m à partir de tissu
WO2023076345A1 (fr) 2021-10-26 2023-05-04 10X Genomics, Inc. Procédés d'analyse spatiale utilisant la capture ciblée d'arn
WO2023086880A1 (fr) 2021-11-10 2023-05-19 10X Genomics, Inc. Procédés, compositions et kits pour déterminer l'emplacement d'un analyte dans un échantillon biologique
WO2023086824A1 (fr) 2021-11-10 2023-05-19 10X Genomics, Inc. Procédés d'identification de molécules de liaison à l'antigène
WO2023102313A1 (fr) 2021-11-30 2023-06-08 10X Genomics, Inc. Systèmes et procédés d'identification de régions d'aneuploïdie dans un tissu
WO2023102118A2 (fr) 2021-12-01 2023-06-08 10X Genomics, Inc. Procédés, compositions et systèmes pour la détection améliorée d'analytes in situ et analyse spatiale
WO2023122033A1 (fr) 2021-12-20 2023-06-29 10X Genomics, Inc. Auto-test pour dispositif d'imagerie
WO2023150171A1 (fr) 2022-02-01 2023-08-10 10X Genomics, Inc. Procédés, compositions et systèmes de capture d'analytes à partir d'échantillons de glioblastome
WO2023150163A1 (fr) 2022-02-01 2023-08-10 10X Genomics, Inc. Procédés, compositions et systèmes de capture d'analytes à partir de tissu lymphatique
WO2023150098A1 (fr) 2022-02-01 2023-08-10 10X Genomics, Inc. Procédés, kits, compositions et systèmes d'analyse spatiale
WO2023159028A1 (fr) 2022-02-15 2023-08-24 10X Genomics, Inc. Systèmes et procédés d'analyse spatiale d'analytes à l'aide d'un alignement de repères
WO2023172670A2 (fr) 2022-03-11 2023-09-14 10X Genomics, Inc. Appareil de manipulation d'échantillon et procédés système de distribution de fluide
WO2023201235A2 (fr) 2022-04-12 2023-10-19 10X Genomics, Inc. Compositions et procédés de génération et de caractérisation de molécules de liaison à un antigène recombinant
WO2023215612A1 (fr) 2022-05-06 2023-11-09 10X Genomics, Inc. Analyse d'un antigène et des interactions antigène-récepteur
WO2023215552A1 (fr) 2022-05-06 2023-11-09 10X Genomics, Inc. Lecteurs de codes-barres moléculaires pour détection d'analytes
WO2023225519A1 (fr) 2022-05-17 2023-11-23 10X Genomics, Inc. Transposons modifiés, compositions et utilisations de ceux-ci
WO2023225366A1 (fr) * 2022-05-20 2023-11-23 Cellanome, Inc. Procédé de construction d'une surface spatiallement codée
WO2023229988A1 (fr) 2022-05-23 2023-11-30 10X Genomics, Inc. Moule pour échantillon de tissu
WO2023229982A2 (fr) 2022-05-24 2023-11-30 10X Genomics, Inc. Confinement de structure poreuse pour suppression de convection
WO2023250077A1 (fr) 2022-06-22 2023-12-28 10X Genomics, Inc. Procédés, compositions et systèmes pour la capture de sondes et/ou de code-barres
WO2024006799A1 (fr) 2022-06-29 2024-01-04 10X Genomics, Inc. Liaison covalente d'oligonucléotides à pont pour la génération d'un réseau moléculaire au moyen d'une ligation
US20240076722A1 (en) 2022-06-29 2024-03-07 10X Genomics, Inc. Compositions and methods for oligonucleotide inversion on arrays
US20240117338A1 (en) 2022-06-29 2024-04-11 10X Genomics, Inc. Methods and compositions for refining feature boundaries in molecular arrays
WO2024006830A1 (fr) 2022-06-29 2024-01-04 10X Genomics, Inc. Procédés et compositions de production de matrice moléculaire à motifs par distribution de billes dirigée
US20240076721A1 (en) 2022-06-29 2024-03-07 10X Genomics, Inc. Method of generating arrays using microfluidics and photolithography
US20240026444A1 (en) 2022-06-29 2024-01-25 10X Genomics, Inc. Compositions and methods for generating molecular arrays using oligonucleotide printing and photolithography
US20240060127A1 (en) 2022-06-29 2024-02-22 10X Genomics, Inc. Methods and systems for light-controlled surface patterning using photomasks
WO2024006798A1 (fr) 2022-06-29 2024-01-04 10X Genomics, Inc. Génération de caractéristiques de réseau moléculaire à haute définition à l'aide d'une résine photosensible
WO2024015862A1 (fr) 2022-07-13 2024-01-18 10X Genomics, Inc. Procédés de caractérisation de molécules de liaison à l'antigène à partir d'échantillons biologiques
WO2024015578A1 (fr) 2022-07-15 2024-01-18 10X Genomics, Inc. Procédés pour déterminer l'emplacement d'un acide nucléique cible dans un échantillon biologique
US20240052404A1 (en) 2022-08-05 2024-02-15 10X Genomics, Inc. Systems and methods for immunofluorescence quantification
WO2024036191A1 (fr) 2022-08-10 2024-02-15 10X Genomics, Inc. Systèmes et procédés de colocalisation
WO2024035844A1 (fr) 2022-08-12 2024-02-15 10X Genomics, Inc. Procédés de réduction de la capture d'analytes
WO2024044703A1 (fr) 2022-08-24 2024-02-29 10X Genomics, Inc. Compositions et procédés de cartographie d'épitopes antigéniques dans des échantillons biologiques
WO2024081212A1 (fr) 2022-10-10 2024-04-18 10X Genomics, Inc. Transcription in vitro d'acides nucléiques capturés spatialement
WO2024081869A1 (fr) 2022-10-14 2024-04-18 10X Genomics, Inc. Procédés d'analyse d'échantillons biologiques

Family Cites Families (944)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3982306A (en) 1975-07-07 1976-09-28 Curry Walter J Fastener for plastic film sheeting and the like
US4514388A (en) 1983-03-22 1985-04-30 Psaledakis Nicholas G Proteolytic enzymes in the zymogen form to treat sarcoma cells
US4574729A (en) 1984-08-06 1986-03-11 E. I. Du Pont De Nemours & Co. Chamber block for a cytocentrifuge having centrifugal force responsive supernatant withdrawal means
US5061049A (en) 1984-08-31 1991-10-29 Texas Instruments Incorporated Spatial light modulator and method
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4883867A (en) 1985-11-01 1989-11-28 Becton, Dickinson And Company Detection of reticulocytes, RNA or DNA
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5589173A (en) 1986-11-04 1996-12-31 Genentech, Inc. Method and therapeutic compositions for the treatment of myocardial infarction
US5525464A (en) 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US4968601A (en) 1988-02-09 1990-11-06 The United States Of America As Represented By The Dept. Of Health & Human Services Method for diagnosing latent viral infection
GB8810400D0 (en) 1988-05-03 1988-06-08 Southern E Analysing polynucleotide sequences
US4988617A (en) 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
AU2684488A (en) 1988-06-27 1990-01-04 Carter-Wallace, Inc. Test device and method for colored particle immunoassay
US5512439A (en) 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
US5002882A (en) 1989-04-27 1991-03-26 New England Biolabs, Inc. Method for producing the XmaI restriction endonuclease and methylase
RU2145635C1 (ru) 1989-05-18 2000-02-20 Чирон Корпорейшн Олигомер (варианты), способ обнаружения последовательности hcv (варианты), набор для обнаружения, способ получения крови
WO1991006678A1 (fr) 1989-10-26 1991-05-16 Sri International Sequençage d'adn
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5559032A (en) 1990-06-29 1996-09-24 Pomeroy; Patrick C. Method and apparatus for post-transfer assaying of material on solid support
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5183053A (en) 1991-04-12 1993-02-02 Acuderm, Inc. Elliptical biopsy punch
WO1993004199A2 (fr) 1991-08-20 1993-03-04 Scientific Generics Limited Procedes de detection et de quantification d'acides nucleiques, et de production d'acides nucleiques marques, immobilises
US5474796A (en) 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
US6872816B1 (en) 1996-01-24 2005-03-29 Third Wave Technologies, Inc. Nucleic acid detection kits
US6759226B1 (en) 2000-05-24 2004-07-06 Third Wave Technologies, Inc. Enzymes for the detection of specific nucleic acid sequences
CA2119126C (fr) 1991-09-16 1996-09-03 Stephen T. Yue Dimeres de colorants a base de cyanine asymetrique
US5321130A (en) 1992-02-10 1994-06-14 Molecular Probes, Inc. Unsymmetrical cyanine dyes with a cationic side chain
US5308751A (en) 1992-03-23 1994-05-03 General Atomics Method for sequencing double-stranded DNA
US5503980A (en) 1992-11-06 1996-04-02 Trustees Of Boston University Positional sequencing by hybridization
US5472881A (en) 1992-11-12 1995-12-05 University Of Utah Research Foundation Thiol labeling of DNA for attachment to gold surfaces
US5410030A (en) 1993-04-05 1995-04-25 Molecular Probes, Inc. Dimers of unsymmetrical cyanine dyes containing pyridinium moieties
US5436134A (en) 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5658751A (en) 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5837832A (en) 1993-06-25 1998-11-17 Affymetrix, Inc. Arrays of nucleic acid probes on biological chips
US5874239A (en) 1993-07-30 1999-02-23 Affymax Technologies N.V. Biotinylation of proteins
US6401267B1 (en) 1993-09-27 2002-06-11 Radoje Drmanac Methods and compositions for efficient nucleic acid sequencing
US5610287A (en) 1993-12-06 1997-03-11 Molecular Tool, Inc. Method for immobilizing nucleic acid molecules
SE9400522D0 (sv) 1994-02-16 1994-02-16 Ulf Landegren Method and reagent for detecting specific nucleotide sequences
US5512462A (en) 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
US6015880A (en) 1994-03-16 2000-01-18 California Institute Of Technology Method and substrate for performing multiple sequential reactions on a matrix
JPH09510351A (ja) 1994-03-16 1997-10-21 ジェン−プローブ・インコーポレイテッド 等温鎖置換核酸増幅法
US5552278A (en) 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
EP0777749B1 (fr) 1994-08-19 2002-10-30 PE Corporation (NY) Procede de ligature et d'amplification associees
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
KR960022566A (ko) 1994-12-30 1996-07-18 김충환 신규한 아미노올리고당 유도체 및 그의 제조방법
US5736351A (en) 1995-01-09 1998-04-07 New Horizons Diagnostics Corporation Method for detection of contaminants
US5959098A (en) 1996-04-17 1999-09-28 Affymetrix, Inc. Substrate preparation process
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5648245A (en) 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
CN1305185C (zh) 1995-06-02 2007-03-14 松下电器产业株式会社 激光光源
US5928928A (en) 1995-06-07 1999-07-27 Universiteit Van Amsterdam Human chitinase, its recombinant production, its use for decomposing chitin, its use in therapy or prophylaxis against infection diseases
SE504798C2 (sv) 1995-06-16 1997-04-28 Ulf Landegren Immunanalys och testkit med två reagens som kan tvärbindas om de adsorberats till analyten
WO1997013845A2 (fr) 1995-10-13 1997-04-17 President And Fellows Of Harvard College Transferases de phosphopantetheinyles et leurs utilisations
US5716825A (en) 1995-11-01 1998-02-10 Hewlett Packard Company Integrated nucleic acid analysis system for MALDI-TOF MS
US5763175A (en) 1995-11-17 1998-06-09 Lynx Therapeutics, Inc. Simultaneous sequencing of tagged polynucleotides
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US6300063B1 (en) 1995-11-29 2001-10-09 Affymetrix, Inc. Polymorphism detection
EP0880598A4 (fr) 1996-01-23 2005-02-23 Affymetrix Inc Evaluation rapide de difference d'abondance d'acides nucleiques, avec un systeme d'oligonucleotides haute densite
US5985557A (en) 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
US6875572B2 (en) 1996-01-24 2005-04-05 Third Wave Technologies, Inc. Nucleic acid detection assays
US6913881B1 (en) 1996-01-24 2005-07-05 Third Wave Technologies, Inc. Methods and compositions for detecting target sequences
AU735440B2 (en) 1996-02-09 2001-07-05 Cornell Research Foundation Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6013440A (en) 1996-03-11 2000-01-11 Affymetrix, Inc. Nucleic acid affinity columns
US5928906A (en) 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification
KR100471341B1 (ko) 1996-05-23 2005-07-21 제네시스 테크놀로지 가부시키가이샤 콘택트프로브및그것을구비한프로브장치
EP0912761A4 (fr) 1996-05-29 2004-06-09 Cornell Res Foundation Inc Detection de differences dans des sequences d'acides nucleiques utilisant une combinaison de la detection par ligase et de reactions d'amplification en chaine par polymerase
US20050003431A1 (en) 1996-08-16 2005-01-06 Wucherpfennig Kai W. Monovalent, multivalent, and multimeric MHC binding domain fusion proteins and conjugates, and uses therefor
US5965443A (en) 1996-09-09 1999-10-12 Wisconsin Alumni Research Foundation System for in vitro transposition
US5925545A (en) 1996-09-09 1999-07-20 Wisconsin Alumni Research Foundation System for in vitro transposition
US6221654B1 (en) 1996-09-25 2001-04-24 California Institute Of Technology Method and apparatus for analysis and sorting of polynucleotides based on size
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
DE19639673A1 (de) 1996-09-27 1998-04-09 Daimler Benz Ag In einem Kraftfahrzeug im Bereich der Frontscheibe angeordnetes Display
US6083761A (en) 1996-12-02 2000-07-04 Glaxo Wellcome Inc. Method and apparatus for transferring and combining reagents
US6060240A (en) 1996-12-13 2000-05-09 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
US5837466A (en) 1996-12-16 1998-11-17 Vysis, Inc. Devices and methods for detecting nucleic acid analytes in samples
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US6737236B1 (en) 1997-01-08 2004-05-18 Proligo, Llc Bioconjugation of macromolecules
US6309824B1 (en) 1997-01-16 2001-10-30 Hyseq, Inc. Methods for analyzing a target nucleic acid using immobilized heterogeneous mixtures of oligonucleotide probes
US8207093B2 (en) 1997-01-21 2012-06-26 The General Hospital Corporation Selection of proteins using RNA-protein fusions
DK0971946T3 (da) 1997-01-21 2006-10-30 Gen Hospital Corp Selektion af proteiner ved anvendelse af RNA-protein-fusioner
US6261804B1 (en) 1997-01-21 2001-07-17 The General Hospital Corporation Selection of proteins using RNA-protein fusions
US5837860A (en) 1997-03-05 1998-11-17 Molecular Tool, Inc. Covalent attachment of nucleic acid molecules onto solid-phases via disulfide bonds
US6327410B1 (en) 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
US6023540A (en) 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
KR20010005544A (ko) 1997-03-21 2001-01-15 그레그 펄쓰 Vntr 대립 유전자의 추출 및 이용방법
EP3034626A1 (fr) 1997-04-01 2016-06-22 Illumina Cambridge Limited Methode de séquencage d'acide nucléique
AU6846798A (en) 1997-04-01 1998-10-22 Glaxo Group Limited Method of nucleic acid sequencing
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
US5919626A (en) 1997-06-06 1999-07-06 Orchid Bio Computer, Inc. Attachment of unmodified nucleic acids to silanized solid phase surfaces
WO1999005295A1 (fr) 1997-07-25 1999-02-04 Thomas Jefferson University Composition et procede d'integration ciblee dans des cellules
WO1999009217A1 (fr) 1997-08-15 1999-02-25 Hyseq, Inc. Procedes et compositions de detection ou de quantification d'especes d'acides nucleiques
GB9718455D0 (en) 1997-09-02 1997-11-05 Mcgregor Duncan P Chimeric binding peptide library screening method
JP2001519538A (ja) 1997-10-10 2001-10-23 プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ 核酸アレイのレプリカ増幅
US6054274A (en) 1997-11-12 2000-04-25 Hewlett-Packard Company Method of amplifying the signal of target nucleic acid sequence analyte
US6242246B1 (en) 1997-12-15 2001-06-05 Somalogic, Inc. Nucleic acid ligand diagnostic Biochip
AU2003200718B2 (en) 1997-12-15 2006-10-19 Somalogic, Inc. Nucleic acid ligand diagnostic biochip
EP1600512B1 (fr) 1997-12-22 2008-05-28 Hitachi Chemical Co., Ltd. Purification d'ARNm sur microplaques
US6844158B1 (en) 1997-12-22 2005-01-18 Hitachi Chemical Co., Ltd. Direct RT-PCR on oligonucleotide-immobilized PCR microplates
US7427678B2 (en) 1998-01-08 2008-09-23 Sigma-Aldrich Co. Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method
DE69942272D1 (de) 1998-02-23 2010-06-02 Wisconsin Alumni Res Found Deranordnungen
CA2318789C (fr) 1998-02-25 2011-05-10 The United States Of America As Represented By The Secretary Department Of Health And Human Services Arrangements cellulaires permettant une definition de profil moleculaire rapide
US6699710B1 (en) 1998-02-25 2004-03-02 The United States Of America As Represented By The Department Of Health And Human Services Tumor tissue microarrays for rapid molecular profiling
WO1999044063A2 (fr) 1998-02-25 1999-09-02 The United States Of America, Represented By The Secretary, Department Of Health And Human Services Micro-arrangements de tissus tumoraux permettant une definition de profil moleculaire rapide
WO1999053102A1 (fr) 1998-04-16 1999-10-21 Packard Bioscience Company Analyse de sequence de polynucleotides
US6906245B1 (en) 1998-04-30 2005-06-14 Sumitomo Chemical Company, Limited Method for producing transgenic plants resistant to weed control compounds which disrupt the porphyrin pathways of plants
US6358475B1 (en) 1998-05-27 2002-03-19 Becton, Dickinson And Company Device for preparing thin liquid for microscopic analysis
US6295153B1 (en) 1998-06-04 2001-09-25 Board Of Regents, The University Of Texas System Digital optical chemistry micromirror imager
AU754952B2 (en) 1998-06-24 2002-11-28 Illumina, Inc. Decoding of array sensors with microspheres
WO2000000637A2 (fr) 1998-06-26 2000-01-06 Visible Genetics Inc. Procede de sequençage d'acides nucleiques, avec un taux reduit d'erreurs
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US20040106110A1 (en) 1998-07-30 2004-06-03 Solexa, Ltd. Preparation of polynucleotide arrays
US20030022207A1 (en) 1998-10-16 2003-01-30 Solexa, Ltd. Arrayed polynucleotides and their use in genome analysis
CA2343359C (fr) 1998-09-18 2008-12-23 Micromet Ag Amplification d'une unique cellule
US6159736A (en) 1998-09-23 2000-12-12 Wisconsin Alumni Research Foundation Method for making insertional mutations using a Tn5 synaptic complex
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6573043B1 (en) 1998-10-07 2003-06-03 Genentech, Inc. Tissue analysis and kits therefor
US6337472B1 (en) 1998-10-19 2002-01-08 The University Of Texas System Board Of Regents Light imaging microscope having spatially resolved images
JP5213202B2 (ja) 1998-10-28 2013-06-19 バイシス・インコーポレーテツド 細胞アレイならびに遺伝障害マーカーの検出および使用方法
US6391937B1 (en) 1998-11-25 2002-05-21 Motorola, Inc. Polyacrylamide hydrogels and hydrogel arrays made from polyacrylamide reactive prepolymers
WO2000032823A1 (fr) 1998-12-02 2000-06-08 Phylos, Inc. Fusions de proteine-adn et leurs utilisations
US6818418B1 (en) 1998-12-10 2004-11-16 Compound Therapeutics, Inc. Protein scaffolds for antibody mimics and other binding proteins
CA2355816C (fr) 1998-12-14 2007-10-30 Li-Cor, Inc. Systeme et procede de sequencage d'acides nucleiques mono-moleculaires par synthese de polymerase
US6830884B1 (en) 1998-12-15 2004-12-14 Molecular Staging Inc. Method of amplification
EP1144684B1 (fr) 1999-01-06 2009-08-19 Callida Genomics, Inc. Sequencage par hybridation, ameliore, utilisant des groupes de sondes
GB9901475D0 (en) 1999-01-22 1999-03-17 Pyrosequencing Ab A method of DNA sequencing
US6565727B1 (en) 1999-01-25 2003-05-20 Nanolytics, Inc. Actuators for microfluidics without moving parts
EP1024201B1 (fr) 1999-01-27 2003-11-26 Commissariat A L'energie Atomique Microéssais pour l'analyse en série de l'expression des gènes et leur applications
US20020150909A1 (en) 1999-02-09 2002-10-17 Stuelpnagel John R. Automated information processing in randomly ordered arrays
US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
US6153389A (en) 1999-02-22 2000-11-28 Haarer; Brian K. DNA additives as a mechanism for unambiguously marking biological samples
CA2370976C (fr) 1999-04-20 2009-10-20 Illumina, Inc. Detection de reactions d'acide nucleique sur microsupports de billes en reseau
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US20050191698A1 (en) 1999-04-20 2005-09-01 Illumina, Inc. Nucleic acid sequencing using microsphere arrays
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
US6673620B1 (en) 1999-04-20 2004-01-06 Cytologix Corporation Fluid exchange in a chamber on a microscope slide
EP1171636A2 (fr) 1999-04-22 2002-01-16 Albert Einstein College Of Medicine Of Yeshiva University Recherche de compositions d'expression genique par la methode multi-fluor fish
US7276336B1 (en) 1999-07-22 2007-10-02 Agilent Technologies, Inc. Methods of fabricating an addressable array of biopolymer probes
US20010055764A1 (en) 1999-05-07 2001-12-27 Empedocles Stephen A. Microarray methods utilizing semiconductor nanocrystals
US6544732B1 (en) 1999-05-20 2003-04-08 Illumina, Inc. Encoding and decoding of array sensors utilizing nanocrystals
US6620584B1 (en) 1999-05-20 2003-09-16 Illumina Combinatorial decoding of random nucleic acid arrays
US6136592A (en) 1999-06-25 2000-10-24 Leighton; Stephen B. Multiple micro-arrays
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6465183B2 (en) 1999-07-01 2002-10-15 Agilent Technologies, Inc. Multidentate arrays
CA2379132C (fr) 1999-07-14 2011-05-31 Packard Bioscience Company Procede d'analyse multiplexee, dans un meme echantillon, de plusieurs sequences d'acides nucleiques cibles
WO2001007915A2 (fr) 1999-07-26 2001-02-01 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health & Human Services, The National Institutes Of Health Methode et dispositif d'analyse de specimens biologiques
WO2001009363A1 (fr) 1999-08-02 2001-02-08 Wisconsin Alumni Research Foundation Enzymes tn5 transposases mutantes et procede d'utilisation de ces enzymes
ATE318932T1 (de) 1999-08-13 2006-03-15 Univ Yale Binär kodierte sequenzmarker
WO2001012862A2 (fr) 1999-08-18 2001-02-22 Illumina, Inc. Compositions et procedes de preparation de solutions d'oligonucleotides
AU2246601A (en) 1999-08-30 2001-04-10 Illumina, Inc. Methods for improving signal detection from an array
KR100527265B1 (ko) 1999-09-13 2005-11-09 뉴젠 테크놀로지스 인코포레이티드 폴리뉴클레오티드 서열의 선형 등온 증폭을 위한 방법 및조성물
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6544790B1 (en) 1999-09-17 2003-04-08 Whitehead Institute For Biomedical Research Reverse transfection method
US6291180B1 (en) 1999-09-29 2001-09-18 American Registry Of Pathology Ultrasound-mediated high-speed biological reaction and tissue processing
US6677160B1 (en) 1999-09-29 2004-01-13 Pharmacia & Upjohn Company Methods for creating a compound library and identifying lead chemical templates and ligands for target molecules
WO2001023610A2 (fr) 1999-09-29 2001-04-05 Solexa Ltd. Sequençage de polynucleotides
ATE432993T1 (de) 1999-10-04 2009-06-15 Univ New Jersey Med Tar rna bindende peptide
CA2386791A1 (fr) 1999-10-08 2001-04-19 Protogene Laboratories, Inc. Procede et appareil destines a produire un grand nombre de reactions au moyen d'une plaque matrice
KR20020071853A (ko) 1999-10-13 2002-09-13 시그나츄어 바이오사이언스 인코포레이티드 테스트 샘플에서 분자 이벤트를 탐지 및 규명하는 시스템및 방법
US7585632B2 (en) 1999-10-29 2009-09-08 Hologic, Inc. Compositions and methods for the detection of a nucleic acid using a cleavage reaction
US6569674B1 (en) 1999-12-15 2003-05-27 Amersham Biosciences Ab Method and apparatus for performing biological reactions on a substrate surface
CA2394358A1 (fr) 1999-12-13 2001-06-14 The Government Of The United States Of America, As Represented By The Se Cretary, Department Of Health & Human Services, The National Institutes Technologie de micro-matrice de tissus a haut debit et applications
US6248535B1 (en) 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
US6485926B2 (en) 1999-12-22 2002-11-26 Fuji Photo Film Co., Ltd. Method for measuring protease activity
US6713298B2 (en) 2000-01-31 2004-03-30 Board Of Regents, The University Of Texas System Method and apparatus for the delivery of samples to a chemical sensor array
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US8076063B2 (en) 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
ATE411397T1 (de) 2000-02-07 2008-10-15 Illumina Inc Nukleinsäure-nachweisverfahren mit universellem priming
US7361488B2 (en) 2000-02-07 2008-04-22 Illumina, Inc. Nucleic acid detection methods using universal priming
DE60127939T2 (de) 2000-02-07 2008-01-24 Illumina, Inc., San Diego Nukleinsäure-Nachweisverfahren mit universellem Priming
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US20010026919A1 (en) 2000-02-08 2001-10-04 Alex Chenchik Nucleic acid assays employing universal arrays
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
AU3839101A (en) 2000-02-15 2001-08-27 Lynx Therapeutics, Inc. Data analysis and display system for ligation-based dna sequencing
US7306904B2 (en) 2000-02-18 2007-12-11 Olink Ab Methods and kits for proximity probing
JP2001284267A (ja) 2000-04-03 2001-10-12 Canon Inc 排気処理方法、プラズマ処理方法及びプラズマ処理装置
WO2001077684A2 (fr) 2000-04-10 2001-10-18 The Scripps Research Institute Analyse proteomique
US6368801B1 (en) 2000-04-12 2002-04-09 Molecular Staging, Inc. Detection and amplification of RNA using target-mediated ligation of DNA by RNA ligase
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
US6291187B1 (en) 2000-05-12 2001-09-18 Molecular Staging, Inc. Poly-primed amplification of nucleic acid sequences
AU2001274869A1 (en) 2000-05-20 2001-12-03 The Regents Of The University Of Michigan Method of producing a dna library using positional amplification
US6511809B2 (en) 2000-06-13 2003-01-28 E. I. Du Pont De Nemours And Company Method for the detection of an analyte by means of a nucleic acid reporter
US7439016B1 (en) 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
JP4744778B2 (ja) 2000-06-21 2011-08-10 バイオアレイ ソルーションズ リミテッド 特定のランダム粒子アレイを適用した複数の被検体分子の分析方法
US8278034B2 (en) 2000-06-22 2012-10-02 Nuclea Biotechnologies, Inc. Methods of making frozen tissue microarrays
US6323009B1 (en) 2000-06-28 2001-11-27 Molecular Staging, Inc. Multiply-primed amplification of nucleic acid sequences
US6581627B2 (en) 2000-06-30 2003-06-24 Jack R. Dillon Dialysis wall station
DE60131194T2 (de) 2000-07-07 2008-08-07 Visigen Biotechnologies, Inc., Bellaire Sequenzbestimmung in echtzeit
GB0018120D0 (en) 2000-07-24 2000-09-13 Fermentas Ab Nuclease
WO2002007503A1 (fr) 2000-07-25 2002-01-31 The Regents Of The University Of California Micropompage par electromouillage
US7613571B2 (en) 2000-07-28 2009-11-03 Doyle Michael D Method and system for the multidimensional morphological reconstruction of genome expression activity
ATE330224T1 (de) 2000-08-15 2006-07-15 Discerna Ltd Funktionelles protein array
SI1918298T1 (sl) 2000-08-21 2011-01-31 Apitope Technology Bristol Ltd Peptid
US6713257B2 (en) 2000-08-25 2004-03-30 Rosetta Inpharmatics Llc Gene discovery using microarrays
US6773566B2 (en) 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
US6942970B2 (en) 2000-09-14 2005-09-13 Zymed Laboratories, Inc. Identifying subjects suitable for topoisomerase II inhibitor treatment
US20020168639A1 (en) 2000-09-22 2002-11-14 Muraca Patrick J. Profile array substrates
AU2001293163A1 (en) 2000-09-27 2002-04-08 Lynx Therapeutics, Inc. Method for determining relative abundance of nucleic acid sequences
AT411066B (de) 2000-10-24 2003-09-25 Steiner Georg E Verfahren und anordnung zur untersuchung von zellen
EP1348034B1 (fr) 2000-11-15 2016-07-20 Minerva Biotechnologies Corporation Identifieurs d'oligonucleotides
EP2381116A1 (fr) 2000-11-16 2011-10-26 California Institute of Technology Appareil et procédés pour effectuer des analyses et criblage à haut rendement
WO2002044425A2 (fr) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Synthese d'acides nucleiques d'enzymes, et compositions et methodes modifiant la fidelite d'incorporation de monomeres
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US20030215936A1 (en) 2000-12-13 2003-11-20 Olli Kallioniemi High-throughput tissue microarray technology and applications
US20030017451A1 (en) 2000-12-21 2003-01-23 Hui Wang Methods for detecting transcripts
US7135296B2 (en) 2000-12-28 2006-11-14 Mds Inc. Elemental analysis of tagged biologically active materials
JP4061043B2 (ja) 2000-12-28 2008-03-12 株式会社ポストゲノム研究所 invitro転写/翻訳系によるペプチド等の製造方法
ATE538380T1 (de) 2001-01-23 2012-01-15 Harvard College Nukleinsäure-programmierbare protein-arrays
EP1356117A2 (fr) 2001-01-25 2003-10-29 TM Bioscience Corporation Polynucleotides pouvant etre utilises comme marqueurs ou complements de marqueurs, production et utilisation desdits polynucleotides
US20030087232A1 (en) 2001-01-25 2003-05-08 Fred Christians Methods for screening polypeptides
KR20020063359A (ko) 2001-01-27 2002-08-03 일렉트론 바이오 (주) 핵산 및 올리거뉴클레오티드의 상보적 이중결합의 특정서열에 특이적으로 반응하는 절단기법을 이용한 핵산 혼성분석 방법 및 장치
EP1379549A2 (fr) 2001-02-07 2004-01-14 Institut Pasteur Sequence du genome de photorhabdus luminescens souche tto1 et utilisations
US6573051B2 (en) 2001-03-09 2003-06-03 Molecular Staging, Inc. Open circle probes with intramolecular stem structures
WO2002072892A1 (fr) 2001-03-12 2002-09-19 California Institute Of Technology Procedes et appareil d'analyse de sequences de polynucleotide par extension de base asynchrone
AU785425B2 (en) 2001-03-30 2007-05-17 Genetic Technologies Limited Methods of genomic analysis
ES2400448T3 (es) 2001-04-30 2013-04-10 Ventana Medical Systems, Inc. Reactivos y métodos para la hibridación automatizada in situ o para microarray
WO2003002979A2 (fr) 2001-06-28 2003-01-09 Illumina, Inc. Decodage multiplex de capteurs d'un ensemble presentant des microspheres
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
WO2003008968A2 (fr) 2001-07-19 2003-01-30 Signet Laboratories, Inc. Procede de criblage de medicaments specifique pour le tissu humain
US20040091857A1 (en) 2001-07-20 2004-05-13 Nallur Girish N. Gene expression profiling
GB0118031D0 (en) 2001-07-24 2001-09-19 Oxford Glycosciences Uk Ltd Self assembled protein nucleic acid complexes and self assembled protein arrays
US7297778B2 (en) 2001-07-25 2007-11-20 Affymetrix, Inc. Complexity management of genomic DNA
DE60210014T2 (de) 2001-07-31 2006-09-21 Pfizer Products Inc., Groton Auf Zellen gegründetes Phosphodiesterase-10A-Assay und Sequenzen
US6696271B2 (en) 2001-08-23 2004-02-24 The Regents Of The University Of California Frozen tissue microarray technology for analysis of RNA, DNA, and proteins
WO2003022028A2 (fr) 2001-09-10 2003-03-20 Meso Scale Technologies, Llc Methodes, reactifs, kits et appareil d'analyse de fonction proteique
US20060188875A1 (en) 2001-09-18 2006-08-24 Perlegen Sciences, Inc. Human genomic polymorphisms
US20040019005A1 (en) 2001-10-01 2004-01-29 Jeffrey Van Ness Methods for parallel measurement of genetic variations
US7195913B2 (en) 2001-10-05 2007-03-27 Surmodics, Inc. Randomly ordered arrays and methods of making and using
US20030148335A1 (en) 2001-10-10 2003-08-07 Li Shen Detecting targets by unique identifier nucleotide tags
US6942972B2 (en) 2001-10-24 2005-09-13 Beckman Coulter, Inc. Efficient synthesis of protein-oligonucleotide conjugates
US20030175947A1 (en) 2001-11-05 2003-09-18 Liu Robin Hui Enhanced mixing in microfluidic devices
AU2002365271B2 (en) 2001-11-06 2008-12-11 Agilix Corporation Sensitive coded detection systems
EP1451365A4 (fr) 2001-11-13 2006-09-13 Rubicon Genomics Inc Amplification et sequencage d'adn au moyen de molecules d'adn produite par fragmentation aleatoire
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
EP1450956A2 (fr) 2001-11-26 2004-09-01 Keck Graduate Institute Procede, appareil et article de regulation microfluidique par electromouillage destines a des analyses chimiques, biochimiques, biologiques et analogues
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US7098041B2 (en) 2001-12-11 2006-08-29 Kimberly-Clark Worldwide, Inc. Methods to view and analyze the results from diffraction-based diagnostics
US20030153850A1 (en) 2002-01-16 2003-08-14 Davis Brian J. Method and apparatus for image-guided therapy
US7731909B1 (en) 2002-01-22 2010-06-08 Grace Bio-Labs, Inc. Reaction surface array diagnostic apparatus
WO2003069333A1 (fr) 2002-02-14 2003-08-21 Illumina, Inc. Traitement des donnees automatique dans des matrices organisees de façon aleatoire
US20040002090A1 (en) 2002-03-05 2004-01-01 Pascal Mayer Methods for detecting genome-wide sequence variations associated with a phenotype
US20030170637A1 (en) 2002-03-06 2003-09-11 Pirrung Michael C. Method of analyzing mRNA splice variants
US7166441B2 (en) 2002-03-12 2007-01-23 Perseptive Biosystems Inc. Method and apparatus for the identification and quantification of biomolecules
US7223371B2 (en) 2002-03-14 2007-05-29 Micronics, Inc. Microfluidic channel network device
AU2003228531A1 (en) 2002-04-15 2003-11-03 The Regents Of The University Of California Screening and therapeutic methods for treating circadian rhythm disorders
US20030235851A1 (en) 2002-04-19 2003-12-25 Roberts Richard W. Methods of using sense and/or nonsense suppression to make nucleic acid-peptide display libraries containing peptides with unnatural amino acid residues
PT2151250E (pt) 2002-05-06 2013-12-09 Endocyte Inc Agentes de formação de imagem direccionados para vitamina
EP1540017A4 (fr) 2002-05-09 2006-05-24 Us Genomics Inc Methodes d'analyse d'un acide nucleique
ES2346646T5 (es) 2002-05-30 2018-02-15 The Scripps Research Institute Ligación catalizada con cobre de azidas y acetilenos
WO2003102233A1 (fr) 2002-06-03 2003-12-11 Pamgene B.V. Nouveaux ensembles ordonnés d'échantillons à haute densité et technique d'analyse d'analysat
US7108976B2 (en) 2002-06-17 2006-09-19 Affymetrix, Inc. Complexity management of genomic DNA by locus specific amplification
FR2841063B1 (fr) 2002-06-18 2004-09-17 Commissariat Energie Atomique Dispositif de deplacement de petits volumes de liquide le long d'un micro-catenaire par des forces electrostatiques
JP2005530151A (ja) 2002-06-18 2005-10-06 インヴィトロジェン コーポレーション プレキャスト水和可能分離媒体の低抵抗電気泳動のための方法および装置
US20050019776A1 (en) 2002-06-28 2005-01-27 Callow Matthew James Universal selective genome amplification and universal genotyping system
US20050118616A1 (en) 2002-08-16 2005-06-02 Kawashima Tadashi R. Amplification of target nucleotide sequence without polymerase chain reaction
US7205128B2 (en) 2002-08-16 2007-04-17 Agilent Technologies, Inc. Method for synthesis of the second strand of cDNA
EP1530578B1 (fr) 2002-08-23 2013-03-13 Illumina Cambridge Limited Nucleotides modifies
US20070166725A1 (en) 2006-01-18 2007-07-19 The Regents Of The University Of California Multiplexed diagnostic platform for point-of care pathogen detection
US20040038385A1 (en) 2002-08-26 2004-02-26 Langlois Richard G. System for autonomous monitoring of bioagents
CA2498362C (fr) 2002-09-11 2012-11-06 Temple University - Of The Commonwealth System Of Higher Education Systeme automatise pour separations par electrophorese a haut rendement
US7595883B1 (en) 2002-09-16 2009-09-29 The Board Of Trustees Of The Leland Stanford Junior University Biological analysis arrangement and approach therefor
JP4632788B2 (ja) 2002-09-20 2011-02-16 ニュー・イングランド・バイオラブズ・インコーポレイティッド 核酸のヘリカーゼ依存性増幅
US7662594B2 (en) 2002-09-20 2010-02-16 New England Biolabs, Inc. Helicase-dependent amplification of RNA
US6911132B2 (en) 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
WO2004028955A2 (fr) 2002-09-25 2004-04-08 California Institute Of Technology Integration microfluidique a grande echelle
US20040259105A1 (en) 2002-10-03 2004-12-23 Jian-Bing Fan Multiplex nucleic acid analysis using archived or fixed samples
AU2003291632A1 (en) 2002-10-03 2004-04-23 Epimmune Inc Hla binding peptides and their uses
US20040067492A1 (en) 2002-10-04 2004-04-08 Allan Peng Reverse transcription on microarrays
WO2004041994A1 (fr) 2002-10-31 2004-05-21 University Of Massachusetts Procede et appareil d'enrobage rapide des blocs cellulaires
US7122384B2 (en) 2002-11-06 2006-10-17 E. I. Du Pont De Nemours And Company Resonant light scattering microparticle methods
US7132233B2 (en) 2002-12-12 2006-11-07 Novartis Vaccines And Diagnostics, Inc. Identification of oligonucleotides for the capture, detection and quantitation of West Nile virus
AU2003301061A1 (en) 2002-12-18 2004-07-22 West Virginia University Research Corporation Apparatus and method for edman degradation using a microfluidic system
KR20040062847A (ko) 2003-01-03 2004-07-09 삼성전자주식회사 핵산 어레이의 복제방법
US7547380B2 (en) 2003-01-13 2009-06-16 North Carolina State University Droplet transportation devices and methods having a fluid surface
GB0302058D0 (en) 2003-01-29 2003-02-26 Univ Cranfield Replication of nucleic acid arrays
ES2342665T3 (es) 2003-01-29 2010-07-12 454 Corporation Secuenciacion desde dos extremos.
EP2365095A1 (fr) 2003-02-26 2011-09-14 Callida Genomics, Inc. Analyse de réseau d'ADN aléatoire par hybridation
US7718403B2 (en) 2003-03-07 2010-05-18 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
EP1601450B1 (fr) 2003-03-10 2013-06-05 Expression Pathology, Inc. Preparation liquide de tissus a partir d'echantillons, de tissus et de cellules biologiques issus d'un procede histopathologique
FR2852317B1 (fr) 2003-03-13 2006-08-04 Biopuces a sondes et leurs methodes d'utilisation
CN1300333C (zh) 2003-04-17 2007-02-14 中国人民解放军军事医学科学院放射与辐射医学研究所 炭疽菌诊断基因芯片的制备及应用
US7083980B2 (en) 2003-04-17 2006-08-01 Wisconsin Alumni Research Foundation Tn5 transposase mutants and the use thereof
US7267994B2 (en) 2003-04-28 2007-09-11 Regents Of The University Of California Element-coded affinity tags
US20040219588A1 (en) 2003-04-30 2004-11-04 Masaru Furuta Method for dispensing reagents onto biological samples and method for analyzing biological samples
US7666612B2 (en) 2003-05-23 2010-02-23 Epfl-Ecole Polytechnique Federale De Lausanne Methods for protein labeling based on acyl carrier protein
US20050026188A1 (en) 2003-05-30 2005-02-03 Van Kessel Andrew G. Methods of identifying, characterizing and comparing organism communities
KR100706464B1 (ko) 2003-05-30 2007-04-10 애플라 코포레이션 혼성화 및 에스피알 검출을 위한 장치 및 방법
US7255994B2 (en) 2003-06-10 2007-08-14 Applera Corporation Ligation assay
US20060216775A1 (en) 2003-06-17 2006-09-28 The Regents Of The University Of Califoenia Compositions and methods for analysis and manipulation of enzymes in biosynthetic proteomes
US20050053980A1 (en) 2003-06-20 2005-03-10 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US20070128656A1 (en) 2003-06-26 2007-06-07 University Of South Florida Direct Fluorescent Label Incorporation Via 1st Strand cDNA Synthesis
US7601492B2 (en) 2003-07-03 2009-10-13 The Regents Of The University Of California Genome mapping of functional DNA elements and cellular proteins
JP5183063B2 (ja) 2003-07-05 2013-04-17 ザ ジョンズ ホプキンス ユニバーシティ 遺伝的変異の検出および列挙のための方法ならびに組成物
US20050079520A1 (en) 2003-07-21 2005-04-14 Jie Wu Multiplexed analyte detection
AU2004265635A1 (en) 2003-08-08 2005-02-24 Thomas Jefferson University Method for rapid identification of alternative splicing
US20050031176A1 (en) 2003-08-08 2005-02-10 Hertel Sarah R. Method and apparatus of multi-modality image fusion
US20050037362A1 (en) 2003-08-11 2005-02-17 Eppendorf Array Technologies, S.A. Detection and quantification of siRNA on microarrays
WO2005021794A2 (fr) 2003-09-02 2005-03-10 Keygene N.V. Procedes bases sur l'amplification ou le dosage d'une ligation d'oligonucleotide (ola) permettant de detecter des sequences d'acide nucleique cibles
US20050095627A1 (en) 2003-09-03 2005-05-05 The Salk Institute For Biological Studies Multiple antigen detection assays and reagents
US7824856B2 (en) 2003-09-10 2010-11-02 Althea Technologies, Inc. Expression profiling using microarrays
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
US20050266417A1 (en) 2003-09-12 2005-12-01 Francis Barany Methods for identifying target nucleic acid molecules
DK1670939T3 (da) 2003-09-18 2010-03-01 Nuevolution As Fremgangsmåde til opnåelse af strukturel information om et kodet molekyle og fremgangsmåde til udvælgelse af forbindelser
US7541166B2 (en) 2003-09-19 2009-06-02 Microfluidic Systems, Inc. Sonication to selectively lyse different cell types
US20050064435A1 (en) 2003-09-24 2005-03-24 Xing Su Programmable molecular barcodes
US7534338B2 (en) 2003-10-10 2009-05-19 Protein Discovery, Inc. Methods and devices for concentration and purification of analytes for chemical analysis including matrix-assisted laser desorption/ionization(MALDI) mass spectrometry (MS)
US7328979B2 (en) 2003-11-17 2008-02-12 Koninklijke Philips Electronics N.V. System for manipulation of a body of fluid
WO2005051984A2 (fr) 2003-11-21 2005-06-09 Arena Pharmaceuticals, Inc. Procedes de production de recepteurs couples a la proteine g olfactifs
CA2545006C (fr) 2003-12-12 2013-09-17 Saint Louis University Biocapteurs permettant de detecter des macromolecules et d'autres analytes
US7259258B2 (en) 2003-12-17 2007-08-21 Illumina, Inc. Methods of attaching biological compounds to solid supports using triazine
US20050136414A1 (en) 2003-12-23 2005-06-23 Kevin Gunderson Methods and compositions for making locus-specific arrays
US20050147976A1 (en) 2003-12-29 2005-07-07 Xing Su Methods for determining nucleotide sequence information
US20070014810A1 (en) 2003-12-31 2007-01-18 Denise Baker Inducing cellular immune responses to human papillomavirus using peptide and nucleic acid compositions
EP3175914A1 (fr) 2004-01-07 2017-06-07 Illumina Cambridge Limited Perfectionnements apportés ou se rapportant à des réseaux moléculaires
US7569392B2 (en) 2004-01-08 2009-08-04 Vanderbilt University Multiplex spatial profiling of gene expression
WO2005067648A2 (fr) 2004-01-08 2005-07-28 Vanderbilt University Profilage spatial multiplex d'expression genique
WO2005071110A2 (fr) 2004-01-23 2005-08-04 Lingvitae As Amelioration des reactions de ligature de polynucleotides
FR2866493B1 (fr) 2004-02-16 2010-08-20 Commissariat Energie Atomique Dispositif de controle du deplacement d'une goutte entre deux ou plusieurs substrats solides
US7378242B2 (en) 2004-03-18 2008-05-27 Atom Sciences, Inc. DNA sequence detection by limited primer extension
FR2868638B1 (fr) 2004-03-30 2006-05-19 Sagem Procede d'echange d'informations entre deux reseaux fonctionnant sous des protocoles de routages differents
KR100624420B1 (ko) 2004-04-10 2006-09-19 삼성전자주식회사 마이크로어레이에 대한 정보가 스팟의 형태로 저장되어있는 마이크로어레이, 그를 제조하는 방법 및 그를이용하는 방법
US20050260653A1 (en) 2004-04-14 2005-11-24 Joshua Labaer Nucleic-acid programmable protein arrays
JP4592060B2 (ja) 2004-04-26 2010-12-01 キヤノン株式会社 Pcr増幅反応装置、ならびに、該装置を利用するpcr増幅反応方法
RU2270254C2 (ru) 2004-04-30 2006-02-20 Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук Способ идентификации трансгенных последовательностей днк в растительном материале и продуктах на его основе, набор олигонуклеотидов и биочип для осуществления этого способа
DE102004022263A1 (de) 2004-05-06 2005-12-15 Clondiag Chip Technologies Gmbh Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen
EP1756307A1 (fr) 2004-05-20 2007-02-28 Trillion Genomics Limited Utilisation de sondes a etiquettes de masse aux fins de detection d'acides nucleiques cibles au moyen de la spectrometrie de masse
US7906276B2 (en) 2004-06-30 2011-03-15 Kimberly-Clark Worldwide, Inc. Enzymatic detection techniques
FR2872715B1 (fr) 2004-07-08 2006-11-17 Commissariat Energie Atomique Microreacteur goutte
FR2872809B1 (fr) 2004-07-09 2006-09-15 Commissariat Energie Atomique Methode d'adressage d'electrodes
US7608434B2 (en) 2004-08-04 2009-10-27 Wisconsin Alumni Research Foundation Mutated Tn5 transposase proteins and the use thereof
WO2006073504A2 (fr) 2004-08-04 2006-07-13 President And Fellows Of Harvard College Sequençage des oscillations dans l'anticodon
JP2006058031A (ja) 2004-08-17 2006-03-02 Hitachi High-Technologies Corp 化学分析装置
US7776547B2 (en) 2004-08-26 2010-08-17 Intel Corporation Cellular analysis using Raman surface scanning
US20060228758A1 (en) 2004-09-13 2006-10-12 Xencor, Inc. Analysis of MHC-peptide binding interactions
GB2423819B (en) 2004-09-17 2008-02-06 Pacific Biosciences California Apparatus and method for analysis of molecules
CN100396790C (zh) 2004-09-17 2008-06-25 北京大学 溶液识别、表面寻址蛋白质芯片及其制备和检测方法
US7524672B2 (en) 2004-09-22 2009-04-28 Sandia Corporation Microfluidic microarray systems and methods thereof
US7527970B2 (en) 2004-10-15 2009-05-05 The United States Of America As Represented By The Department Of Health And Human Services Method of identifying active chromatin domains
EP1816192B1 (fr) 2004-10-15 2012-12-12 National Institute of Advanced Industrial Science and Technology Lieur de construction de conjugue arnm-puromycine-proteine
EP2322616A1 (fr) 2004-11-12 2011-05-18 Asuragen, Inc. Procédés et compositions impliquant l'ARNmi et des molécules inhibitrices de l'ARNmi
US7183119B2 (en) 2004-11-15 2007-02-27 Eastman Kodak Company Method for sensitive detection of multiple biological analytes
US7745143B2 (en) 2004-11-19 2010-06-29 Plexera, Llc Plasmon resonance biosensor and method
DK1828381T3 (da) 2004-11-22 2009-02-23 Peter Birk Rasmussen Templatedirigeret split-and-mix-syntese af småmolekylebiblioteker
ITPD20040301A1 (it) 2004-11-26 2005-02-26 Dimensional Srl P Metodo ed apparato per la separazione simultanea di molecole biologiche mediante elettroforesi bidimensionale
GB0427236D0 (en) 2004-12-13 2005-01-12 Solexa Ltd Improved method of nucleotide detection
WO2006064199A1 (fr) 2004-12-13 2006-06-22 Solexa Limited Procede ameliore de detection de nucleotides
US20060292586A1 (en) 2004-12-17 2006-12-28 Schroth Gary P ID-tag complexes, arrays, and methods of use thereof
WO2006074351A2 (fr) 2005-01-05 2006-07-13 Agencourt Personal Genomics Terminateurs nucleotidiques reversibles et leurs utilisations
KR100682920B1 (ko) 2005-01-20 2007-02-15 삼성전자주식회사 다중 바이오어세이용 미세유체 칩 및 그 제조방법
US7458661B2 (en) 2005-01-25 2008-12-02 The Regents Of The University Of California Method and apparatus for promoting the complete transfer of liquid drops from a nozzle
WO2006081222A2 (fr) 2005-01-25 2006-08-03 Compass Genetics, Llc. Amplification isotherme d'adn
CN101916359B (zh) 2005-01-27 2012-06-20 剑桥研究和仪器设备股份有限公司 把样本的不同区域分类到相应类别的方法和设备
EP1859330B1 (fr) 2005-01-28 2012-07-04 Duke University Appareils et procedes de manipulation de gouttelettes sur une carte de circuits imprimes
EP2230315A1 (fr) 2005-02-01 2010-09-22 AB Advanced Genetic Analysis Corporation Sequencage nucléotidique comprenant des cycles sucessifs de fromation duplex
JP2008529035A (ja) 2005-02-03 2008-07-31 パーキンエルマー エルエーエス,インク. 多次元シグナルを用いる超高感度検出システム
US7407757B2 (en) 2005-02-10 2008-08-05 Population Genetics Technologies Genetic analysis by sequence-specific sorting
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
US20060199207A1 (en) 2005-02-24 2006-09-07 Matysiak Stefan M Self-assembly of molecules using combinatorial hybridization
US7727721B2 (en) 2005-03-08 2010-06-01 California Institute Of Technology Hybridization chain reaction amplification for in situ imaging
GB0504774D0 (en) 2005-03-08 2005-04-13 Lingvitae As Method
US7601498B2 (en) 2005-03-17 2009-10-13 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection and associated technology
US7776567B2 (en) 2005-03-17 2010-08-17 Biotium, Inc. Dimeric and trimeric nucleic acid dyes, and associated systems and methods
US7303880B2 (en) 2005-03-18 2007-12-04 Wisconsin Alumni Research Foundation Microdissection-based methods for determining genomic features of single chromosomes
US7229769B2 (en) 2005-03-25 2007-06-12 Illumina, Inc. Compositions and methods for detecting protease activity
ES2313143T3 (es) 2005-04-06 2009-03-01 Maurice Stroun Metodo para el diagnostico de cancer mediante la deteccion de adn y arn circulantes.
GB0508983D0 (en) 2005-05-03 2005-06-08 Oxford Gene Tech Ip Ltd Cell analyser
JP4990886B2 (ja) 2005-05-10 2012-08-01 ソレックサ リミテッド 改良ポリメラーゼ
ATE502120T1 (de) 2005-05-12 2011-04-15 Affymetrix Inc Multiplex-assays für verzweigtkettige dna
WO2006124644A2 (fr) 2005-05-12 2006-11-23 Board Of Regents, The University Of Texas System Etablissement de profils de proteines et d'anticorps au moyen de microreseaux de petites molecules
GB0509833D0 (en) 2005-05-16 2005-06-22 Isis Innovation Cell analysis
CA2608751A1 (fr) 2005-05-18 2006-11-23 Novartis Ag Methodes de diagnostic et de traitement des maladies proliferatives mediees par la signalisation cd40
US20060263789A1 (en) 2005-05-19 2006-11-23 Robert Kincaid Unique identifiers for indicating properties associated with entities to which they are attached, and methods for using
CN100526453C (zh) 2005-05-20 2009-08-12 麦克奥迪实业集团有限公司 激光显微切割后细胞收集方法
CA2609328A1 (fr) 2005-05-26 2006-11-30 The Trustees Of Boston University Quantification d'acides nucleiques et de proteines au moyen d'etiquettes de masse d'oligonucleotides
US8486629B2 (en) 2005-06-01 2013-07-16 Bioarray Solutions, Ltd. Creation of functionalized microparticle libraries by oligonucleotide ligation or elongation
EP1910537A1 (fr) 2005-06-06 2008-04-16 454 Life Sciences Corporation Séquençage d'extrémités appariées
EP1889047B1 (fr) 2005-06-07 2011-08-10 Centre National De La Recherche Scientifique -Cnrs- UTILISATION DE CONJUGUES AVEC DES LIEURS CLIVABLES PAR PHOTODISSOCIATION OU PAR FRAGMENTATION A DES FINS D'ANALYSE PAR SPECTROMETRIE DE MASSE DES coupes de tissu
GB0511717D0 (en) 2005-06-09 2005-07-13 Babraham Inst Repeatable protein arrays
CA2611671C (fr) 2005-06-15 2013-10-08 Callida Genomics, Inc. Reseaux de molecules simples pour analyse genetique et chimique
US7709198B2 (en) 2005-06-20 2010-05-04 Advanced Cell Diagnostics, Inc. Multiplex detection of nucleic acids
US20070087360A1 (en) 2005-06-20 2007-04-19 Boyd Victoria L Methods and compositions for detecting nucleotides
US7873193B2 (en) 2005-06-21 2011-01-18 Carl Zeiss Microimaging Gmbh Serial section analysis for computer-controlled microscopic imaging
DE602006018744D1 (de) 2005-06-23 2011-01-20 Keygene Nv Strategien mit hohem durchsatz zur identifizierung und zum nachweis von polymorphismen
US20070026430A1 (en) 2005-06-30 2007-02-01 Applera Corporation Proximity probing of target proteins comprising restriction and/or extension
US7883848B2 (en) 2005-07-08 2011-02-08 Olink Ab Regulation analysis by cis reactivity, RACR
JP4822753B2 (ja) * 2005-07-11 2011-11-24 一般社団法人オンチップ・セロミクス・コンソーシアム 細胞構成物質分取チップならびに細胞構成物質解析システム及びこれらを用いる細胞構成物質解析法
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US20070020640A1 (en) 2005-07-21 2007-01-25 Mccloskey Megan L Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis
US20070023292A1 (en) 2005-07-26 2007-02-01 The Regents Of The University Of California Small object moving on printed circuit board
US7805081B2 (en) 2005-08-11 2010-09-28 Pacific Biosciences Of California, Inc. Methods and systems for monitoring multiple optical signals from a single source
WO2007027653A1 (fr) 2005-09-01 2007-03-08 Promega Corporation Dosages cellulaires du protéasome luminogène et non luminogène
WO2007030373A2 (fr) 2005-09-07 2007-03-15 St. Jude Children's Research Hospital Procede d'analyse d'hybridation in situ
JP2007074967A (ja) * 2005-09-13 2007-03-29 Canon Inc 識別子プローブ及びそれを用いた核酸増幅方法
CA2623539C (fr) 2005-09-29 2015-12-15 Keygene N.V. Criblage a haut debit de populations mutagenisees
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
WO2007041689A2 (fr) 2005-10-04 2007-04-12 President And Fellows Of Harvard College Procédés de marquage spécifique à un site de molécules et molécules produites par ceux-ci
US7320600B2 (en) 2005-10-25 2008-01-22 Research In Motion Limited Device opener and vibration mechanism
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
US20070116612A1 (en) 2005-11-02 2007-05-24 Biopath Automation, L.L.C. Prefix tissue cassette
EP1951905B1 (fr) 2005-11-10 2012-04-11 Affymetrix, Inc. Détection d'acides nucléiques par l'amplification d'acides nucléiques de substitut
WO2007120208A2 (fr) 2005-11-14 2007-10-25 President And Fellows Of Harvard College Séquençage de l'adn en cercle tournant sur nanogrille
ATE527383T1 (de) 2005-11-22 2011-10-15 Stichting Dienst Landbouwkundi Multiplex-nukleinsäurenachweis
EP1957979A1 (fr) 2005-11-25 2008-08-20 Koninklijke Philips Electronics N.V. Biocapteur magnetique pour la determination d' une activite enzymatique
US9056291B2 (en) * 2005-11-30 2015-06-16 Micronics, Inc. Microfluidic reactor system
WO2007100392A2 (fr) 2005-11-30 2007-09-07 Biotium, Inc. Substrat d'enzyme comprenant un colorant fonctionnel, technologie et procédés associés
US20070161029A1 (en) 2005-12-05 2007-07-12 Panomics, Inc. High throughput profiling of methylation status of promoter regions of genes
US7803751B2 (en) 2005-12-09 2010-09-28 Illumina, Inc. Compositions and methods for detecting phosphomonoester
DE102005060738A1 (de) 2005-12-16 2007-06-21 Qiagen Gmbh Verfahren zur Extraktion von Biomolekülen aus fixierten Geweben
US20070178503A1 (en) 2005-12-19 2007-08-02 Feng Jiang In-situ genomic DNA chip for detection of cancer
US20070141718A1 (en) 2005-12-19 2007-06-21 Bui Huy A Reduction of scan time in imaging mass spectrometry
KR101489804B1 (ko) 2005-12-21 2015-02-05 메소 스케일 테크놀러지즈, 엘엘시 분석 시약을 갖는 분석 모듈 및 그것의 제조 및 사용 방법
ES2394633T3 (es) 2005-12-22 2013-02-04 Keygene N.V. Estrategias mejoradas para elaboración de perfiles de transcritos usando tecnologías de secuenciación de alto rendimiento
DK3404114T3 (da) 2005-12-22 2021-06-28 Keygene Nv Fremgangsmåde til detektering af AFLP-baseret polymorfisme med højt gennemløb
EP1985092B1 (fr) 2005-12-23 2011-07-06 Telefonaktiebolaget LM Ericsson (publ) Procede et appareil de resolution de la congestion du trafic de paquets de donnees
AU2006330834B2 (en) 2005-12-23 2013-09-12 Nanostring Technologies, Inc. Compositions comprising oriented, immobilized macromolecules and methods for their preparation
US20100015607A1 (en) 2005-12-23 2010-01-21 Nanostring Technologies, Inc. Nanoreporters and methods of manufacturing and use thereof
WO2007076726A1 (fr) 2006-01-04 2007-07-12 Si Lok Méthodes pour la cartographie d'acides nucléiques et l'identification de variations structurales fines dans des acides nucléiques et leurs utilisations
US7544473B2 (en) 2006-01-23 2009-06-09 Population Genetics Technologies Ltd. Nucleic acid analysis using sequence tokens
WO2007087312A2 (fr) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Décompte moléculaire
WO2007087339A2 (fr) 2006-01-24 2007-08-02 Perkinelmer Las, Inc. Quantification multiplexée d’un analyte par électrochromatographie planaire en 2d
WO2007092538A2 (fr) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Procédés de confection de sondes nucléotidiques pour séquençage et synthèse
JP5180845B2 (ja) 2006-02-24 2013-04-10 カリダ・ジェノミックス・インコーポレイテッド Dnaアレイ上でのハイスループットゲノム配列決定
SG10201405158QA (en) 2006-02-24 2014-10-30 Callida Genomics Inc High throughput genome sequencing on dna arrays
CN101395280A (zh) 2006-03-01 2009-03-25 凯津公司 基于测序的高通量SNPs连接检测技术
JP2007248396A (ja) 2006-03-17 2007-09-27 Toshiba Corp 核酸検出用デバイスおよび核酸検出装置
EP2021503A1 (fr) 2006-03-17 2009-02-11 Solexa Ltd. Procédés isothermiques pour créer des réseaux moléculaires clonales simples
GB0605584D0 (en) 2006-03-20 2006-04-26 Olink Ab Method for analyte detection using proximity probes
US20070231823A1 (en) 2006-03-23 2007-10-04 Mckernan Kevin J Directed enrichment of genomic DNA for high-throughput sequencing
US8975216B2 (en) 2006-03-30 2015-03-10 Pacific Biosciences Of California Articles having localized molecules disposed thereon and methods of producing same
CN101460953B (zh) 2006-03-31 2012-05-30 索雷克萨公司 用于合成分析的序列的系统和装置
DK3239304T3 (da) 2006-04-04 2020-10-26 Keygene Nv Højgennemløbsdetektering af molekylære markører baseret på AFLP og sekventering med højt gennemløb
JP5054096B2 (ja) 2006-04-18 2012-10-24 アドヴァンスト リキッド ロジック インコーポレイテッド 液滴に基づく生化学
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
US8383338B2 (en) 2006-04-24 2013-02-26 Roche Nimblegen, Inc. Methods and systems for uniform enrichment of genomic regions
US20070254305A1 (en) 2006-04-28 2007-11-01 Nsabp Foundation, Inc. Methods of whole genome or microarray expression profiling using nucleic acids prepared from formalin fixed paraffin embedded tissue
EP2677039B8 (fr) 2006-05-10 2022-10-05 DxTerity Diagnostics Incorporated Détection de cibles d'acides nucléiques au moyen de sondes oligonucléotidiques chimiquement réactives
JP2007304043A (ja) 2006-05-15 2007-11-22 Canon Inc プローブ固定担体の製造方法
CA2653095C (fr) 2006-05-22 2013-07-16 Nanostring Technologies, Inc. Systeme et procedes pour analyser des nanoreporteurs
US20080132429A1 (en) 2006-05-23 2008-06-05 Uchicago Argonne Biological microarrays with enhanced signal yield
US9012239B2 (en) 2006-05-27 2015-04-21 Fluidigm Canada Inc. Polymer backbone element tags
US9149564B2 (en) 2006-06-23 2015-10-06 The Regents Of The University Of California Articles comprising large-surface-area bio-compatible materials and methods for making and using them
EP2032686B1 (fr) * 2006-06-23 2022-01-12 Illumina, Inc. Dispositif et méthode pour la création d'amas ordonnés d'adn
US20090253582A1 (en) 2006-06-29 2009-10-08 Ge Healthcare Bio-Sciences Ab Chamber apparatus
US8178316B2 (en) 2006-06-29 2012-05-15 President And Fellows Of Harvard College Evaluating proteins
US7312029B1 (en) 2006-06-30 2007-12-25 Searete Llc Method of combing an elongated molecule
US8362242B2 (en) 2006-06-30 2013-01-29 Dh Technologies Development Pte. Ltd. Analyte determination utilizing mass tagging reagents comprising a non-encoded detectable label
AT503862B1 (de) 2006-07-05 2010-11-15 Arc Austrian Res Centers Gmbh Pathogen-identifizierung anhand eines 16s oder 18s-rrna mikroarray
CN101490562B (zh) 2006-07-10 2012-12-19 株式会社日立高新技术 液体输送设备
EP2081869B1 (fr) 2006-07-10 2020-11-04 California Institute of Technology Procédé permettant d'ancrer de façon sélective un grand nombre de structures à l'échelle nanométrique
EP1878502A1 (fr) 2006-07-14 2008-01-16 Roche Diagnostics GmbH Appareil pour le chauffage et le refroidissement
CN101522915A (zh) 2006-08-02 2009-09-02 加州理工学院 用于检测和/或分选靶标的方法和系统
US9012390B2 (en) 2006-08-07 2015-04-21 Raindance Technologies, Inc. Fluorocarbon emulsion stabilizing surfactants
WO2008022332A2 (fr) 2006-08-18 2008-02-21 Board Of Regents, The University Of Texas System Système, procédé et kit pour répliquer une puce à adn
US20080047835A1 (en) 2006-08-22 2008-02-28 Macconnell William P Genomic DNA Purifier
EP3936857B1 (fr) 2006-09-01 2023-06-21 Pacific Biosciences Of California, Inc. Substrats, systèmes et procédés d'analyse de matériaux
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
EP2076609A1 (fr) 2006-10-10 2009-07-08 Illumina Inc. Compositions et procédés pour une sélection représentative d'acides nucléiques à partir de mélanges complexes
US8343746B2 (en) 2006-10-23 2013-01-01 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US20080108804A1 (en) 2006-11-02 2008-05-08 Kabushiki Kaisha Dnaform Method for modifying RNAS and preparing DNAS from RNAS
US9266076B2 (en) 2006-11-02 2016-02-23 The Regents Of The University Of California Method and apparatus for real-time feedback control of electrical manipulation of droplets on chip
WO2008069906A2 (fr) 2006-11-14 2008-06-12 The Regents Of The University Of California Expression numérisée de l'analyse génétique
US9201063B2 (en) 2006-11-16 2015-12-01 General Electric Company Sequential analysis of biological samples
US7655898B2 (en) 2006-11-30 2010-02-02 Cambridge Research & Instrumentation, Inc. Optical filter assembly with selectable bandwidth and rejection
KR101433208B1 (ko) 2006-12-13 2014-08-22 루미넥스 코포레이션 실시간으로 pcr의 다중 분석을 위한 시스템과 방법
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
JP5622392B2 (ja) 2006-12-14 2014-11-12 ライフ テクノロジーズ コーポレーション 大規模fetアレイを用いた分析物測定のための方法および装置
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
CN101221182A (zh) 2007-01-08 2008-07-16 山东司马特生物芯片有限公司 一种荧光蛋白质芯片血清肿瘤系列诊断新方法
WO2008093098A2 (fr) 2007-02-02 2008-08-07 Illumina Cambridge Limited Procedes pour indexer des echantillons et sequencer de multiples matrices nucleotidiques
US20080220434A1 (en) 2007-02-07 2008-09-11 Perscitus Biosciences, Llc Detection Of Molecule Proximity
JP2010517583A (ja) 2007-02-12 2010-05-27 プロテオノヴァ、 インコーポレイテッド mRNAに連結された可溶性ランダムポリペプチドのライブラリーの生成
KR100819006B1 (ko) 2007-02-13 2008-04-03 삼성전자주식회사 마이크로 어레이용 마스크 세트, 이의 제조 방법, 및마스크 세트를 이용한 마이크로 어레이의 제조 방법
US8153782B2 (en) 2007-02-14 2012-04-10 Eastman Chemical Company Reformation of ionic liquids
KR101017808B1 (ko) 2007-04-04 2011-02-28 엔에이치엔(주) 편집 파일 자동 생성 방법 및 그 장치
JP5555157B2 (ja) 2007-04-10 2014-07-23 ナノストリング テクノロジーズ, インコーポレイテッド ナノレポーターにおける使用のための標的特異的配列を同定するための方法およびコンピュータシステム
US20090006002A1 (en) 2007-04-13 2009-01-01 Sequenom, Inc. Comparative sequence analysis processes and systems
WO2008147899A1 (fr) 2007-05-23 2008-12-04 Oregon Health & Science University Systèmes de puce à adn et procédés permettant d'identifier des protéines de liaison à l'adn
US20090105959A1 (en) 2007-06-01 2009-04-23 Braverman Michael S System and method for identification of individual samples from a multiplex mixture
WO2008151127A1 (fr) 2007-06-04 2008-12-11 President And Fellows Of Harvard College Composés et procédés pour effectuer une ligature chimique
EP2191897B1 (fr) 2007-06-21 2014-02-26 Gen-Probe Incorporated Instrument et réceptacles pour effectuer les procédés
US8331387B2 (en) 2007-06-22 2012-12-11 Broadcom Corporation Data switching flow control with virtual output queuing
CN101679932A (zh) 2007-06-27 2010-03-24 数字化生物系统 用于热交换化学过程的基于数字微流体的装置
US7934568B2 (en) 2007-06-27 2011-05-03 Myrick Thomas M Rock core removal method and apparatus
US7635566B2 (en) 2007-06-29 2009-12-22 Population Genetics Technologies Ltd. Methods and compositions for isolating nucleic acid sequence variants
US7534991B2 (en) 2007-07-10 2009-05-19 Cambridge Research & Instrumentation, Inc. Athermalized birefringent filter apparatus and method
US20100112590A1 (en) 2007-07-23 2010-05-06 The Chinese University Of Hong Kong Diagnosing Fetal Chromosomal Aneuploidy Using Genomic Sequencing With Enrichment
US20100159446A1 (en) 2007-07-27 2010-06-24 Haff Lawrence A Detection Assays and Use Thereof
JP2009036694A (ja) * 2007-08-03 2009-02-19 Tokyo Medical & Dental Univ 空間分布を保った細胞内生体物質の解析方法
WO2009032167A1 (fr) 2007-08-29 2009-03-12 Illumina Cambridge Procédé de séquençage d'une matrice de polynucléotides
ITBO20070627A1 (it) 2007-09-14 2009-03-15 Twof Inc Metodo per la preparazione di dna microarray con sonde ad alta densita' lineare
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
CA2697640C (fr) 2007-09-21 2016-06-21 Katholieke Universiteit Leuven Outils et procedes pour tests genetiques ayant recours a un sequencage de derniere generation
US8330087B2 (en) 2007-10-16 2012-12-11 Cambridge Research & Instrumentation, Inc. Spectral imaging system with dynamic optical correction
EP2053132A1 (fr) 2007-10-23 2009-04-29 Roche Diagnostics GmbH Enrichissement et analyse de séquence de régions génomiques
US8518640B2 (en) 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
CN101205560A (zh) * 2007-11-30 2008-06-25 李松 基于磁性纳米粒子与通用标签技术的高通量单核苷酸多态性检测方法
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
EP3699291A1 (fr) 2008-01-17 2020-08-26 Sequenom, Inc. Procédés d'analyse de séquence d'acide nucléique à molécule unique et compositions
KR20090081260A (ko) 2008-01-23 2009-07-28 삼성전자주식회사 마이크로 어레이 혼성화 검출 방법
JP5395098B2 (ja) 2008-02-15 2014-01-22 バイオ−ラッド ラボラトリーズ,インコーポレイティド 自動調整する蓋を有するサーマルサイクラー
DE102008014687A1 (de) 2008-03-18 2009-09-24 Smartrac Ip B.V. Lagenverbund für einen Kartenkörper und Verfahren zur Herstellung des Lagenverbunds
US7846666B2 (en) 2008-03-21 2010-12-07 Nugen Technologies, Inc. Methods of RNA amplification in the presence of DNA
US20090253163A1 (en) 2008-04-02 2009-10-08 General Electric Company Iterative staining of biological samples
WO2009132028A1 (fr) * 2008-04-21 2009-10-29 Complete Genomics, Inc. Structures pour puces de détection d'acides nucléiques
DE102008023438B4 (de) 2008-05-14 2011-06-30 Bruker Daltonik GmbH, 28359 Verfahren zur Analyse von Gewebeschnitten
US8093064B2 (en) 2008-05-15 2012-01-10 The Regents Of The University Of California Method for using magnetic particles in droplet microfluidics
DE102008025656B4 (de) 2008-05-28 2016-07-28 Genxpro Gmbh Verfahren zur quantitativen Analyse von Nukleinsäuren, Marker dafür und deren Verwendung
WO2009148560A2 (fr) 2008-05-30 2009-12-10 Board Of Regents, The Universtiy Of Texas System Procédés et compositions pour un séquençage d'acide nucléique
US8199999B2 (en) 2008-06-17 2012-06-12 Cambridge Research & Instrumentation, Inc. Image classifier training
GB0811574D0 (en) 2008-06-24 2008-07-30 Trillion Genomics Ltd Characterising planar samples by mass spectrometry
US8198028B2 (en) 2008-07-02 2012-06-12 Illumina Cambridge Limited Using populations of beads for the fabrication of arrays on surfaces
US20100035249A1 (en) 2008-08-05 2010-02-11 Kabushiki Kaisha Dnaform Rna sequencing and analysis using solid support
US8519115B2 (en) 2008-08-14 2013-08-27 Nanostring Technologies, Inc. Stable nanoreporters
EP2326732A4 (fr) 2008-08-26 2012-11-14 Fluidigm Corp Procédés de dosage pour un débit amélioré d'échantillons et/ou de cibles
US20100055733A1 (en) 2008-09-04 2010-03-04 Lutolf Matthias P Manufacture and uses of reactive microcontact printing of biomolecules on soft hydrogels
EP2163900A1 (fr) 2008-09-04 2010-03-17 Commissariat A L'energie Atomique Nouveau procédé d'imagerie par spectrométrie de masse et nouveaux dérivés de trityle associés à des marqueurs de masse
US8586310B2 (en) 2008-09-05 2013-11-19 Washington University Method for multiplexed nucleic acid patch polymerase chain reaction
US20110244448A1 (en) 2008-09-08 2011-10-06 Masataka Shirai Dna detecting apparatus, dna detecting device and dna detecting method
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
BRPI0823076A2 (pt) 2008-09-19 2015-06-16 Prysmian Spa Cabo de telecomunicação, fibra óptica microestruturada tamponada.
ES2626903T3 (es) 2008-09-22 2017-07-26 Ventana Medical Systems, Inc. Procesamiento selectivo de material biológico en un sustrato de micromatriz
MX2011003380A (es) 2008-09-30 2011-04-21 Abbott Lab Genotecas de anticuerpo mejoradas.
EP2347252B1 (fr) 2008-10-08 2014-07-16 Sage Science, Inc. Système d'électrophorèse préparatoire à canaux multiples
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US9751084B2 (en) 2008-10-28 2017-09-05 Emd Millipore Corporation Biological culture assembly
EP2356259A4 (fr) 2008-10-30 2012-11-14 Sequenom Inc Produits et procédés pour l identification d acide nucléique multiplexée
US9394567B2 (en) 2008-11-07 2016-07-19 Adaptive Biotechnologies Corporation Detection and quantification of sample contamination in immune repertoire analysis
US9365901B2 (en) 2008-11-07 2016-06-14 Adaptive Biotechnologies Corp. Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
JP5798926B2 (ja) 2008-11-07 2015-10-21 シーケンタ インコーポレイテッド 配列解析によって病態をモニターする方法
EP2370803A2 (fr) 2008-12-03 2011-10-05 The United States Government As Represented By The Department Of Veterans Affairs Récupération moléculaire assistée par pression (pamr) de biomolécules, extraction d'antigène assistée par pression (paar) et histologie de tissu assistée par pression (path)
US20110275077A1 (en) 2009-01-12 2011-11-10 William James Oligonucleotide-Coated Affinity Membranes and Uses Thereof
US8790873B2 (en) 2009-01-16 2014-07-29 Affymetrix, Inc. DNA ligation on RNA template
KR101059565B1 (ko) 2009-02-11 2011-08-26 어플라이드 프레시젼, 인코포레이티드 밝은 기준점 표지를 갖는 마이크로어레이 및 그로부터 광 데이터를 수집하는 방법
US8481698B2 (en) 2009-03-19 2013-07-09 The President And Fellows Of Harvard College Parallel proximity ligation event analysis
CA3018687C (fr) 2009-04-02 2021-07-13 Fluidigm Corporation Procede d'amplification a amorce multiple pour codage a barres d'acides nucleiques cibles
CN102625850B (zh) * 2009-04-03 2014-11-26 蒂莫西·Z·刘 多重核酸检测方法和系统
WO2010127186A1 (fr) 2009-04-30 2010-11-04 Prognosys Biosciences, Inc. Produits de construction d'acide nucléique et leurs procédés d'utilisation
AU2010242073C1 (en) 2009-04-30 2015-12-24 Good Start Genetics, Inc. Methods and compositions for evaluating genetic markers
JP5829606B2 (ja) 2009-06-29 2015-12-09 カリフォルニア・インスティテュート・オブ・テクノロジーCalifornia Institute Oftechnology 単一細胞からの未知の再配列されたt細胞受容体の単離
RU2410439C1 (ru) 2009-07-06 2011-01-27 Российская Федерация, От Имени Которой Выступает Министерство Образования И Науки Российской Федерации Способ абляции целевой днк с поверхности днк-биочипов
WO2011008831A2 (fr) 2009-07-14 2011-01-20 University Of Florida Research Foundation, Inc. Marqueurs de masse pour l’analyse spectrométrique d’immunoglobulines
GB0912909D0 (en) 2009-07-23 2009-08-26 Olink Genomics Ab Probes for specific analysis of nucleic acids
KR101029343B1 (ko) 2009-07-30 2011-04-13 한국과학기술연구원 면역분석 기반의 항원 검출용 키트 및 항원 검출 방법
JP2013500725A (ja) 2009-07-31 2013-01-10 プログノシス バイオサイエンシズ インコーポレイテッド アッセイツールおよびその使用方法
WO2011014811A1 (fr) 2009-07-31 2011-02-03 Ibis Biosciences, Inc. Amorces de capture et supports solides liés à une séquence de capture pour tests diagnostiques moléculaires
EP2467479B1 (fr) 2009-08-20 2016-01-06 Population Genetics Technologies Ltd Compositions et procédés de réarrangement d'acide nucléique intramoléculaire
US8496798B2 (en) 2009-09-01 2013-07-30 Oregon Health & Science University Reversible current gel electrophoresis device for separating biological macromolecules
SG169918A1 (en) 2009-10-02 2011-04-29 Fluidigm Corp Microfluidic devices with removable cover and methods of fabrication and application
MX2012004105A (es) 2009-10-09 2012-09-07 Invisible Sentinel Inc Dispositivo para deteccion de antigenos y usos del mismo.
CA2775613C (fr) 2009-10-13 2018-06-12 Nanostring Technologies, Inc. Detection de proteine par l'intermediaire de nanorapporteurs
US9005891B2 (en) 2009-11-10 2015-04-14 Genomic Health, Inc. Methods for depleting RNA from nucleic acid samples
BR112012011181A2 (pt) 2009-11-13 2020-10-13 Ventana Medical Systems, Inc. ''estação de processsamento de peças corrediça automatizada e método de processamento de uma amostra''
US20120277113A1 (en) 2009-11-18 2012-11-01 Ruo-Pan Huang Array-based proximity ligation association assays
CN106701739A (zh) * 2009-12-04 2017-05-24 株式会社日立制作所 基因表达解析用解析装置及设备
SG10201407883PA (en) 2009-12-07 2015-01-29 Illumina Inc Multi-sample indexing for multiplex genotyping
EP2510127B1 (fr) 2009-12-07 2015-06-10 Prognosys Biosciences, Inc. Matrices de présentation de peptide
WO2011075083A1 (fr) 2009-12-15 2011-06-23 Agency For Science, Technology And Research Traitement de fragments d'adn amplifies pour sequencage
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
FR2954907B1 (fr) 2010-01-04 2012-02-24 Oreal Composition cosmetique, procede de traitement cosmetique et kit
US8889416B2 (en) 2010-01-21 2014-11-18 California Institute Of Technology Methods and devices for micro-isolation, extraction, and/or analysis of microscale components
WO2011094669A1 (fr) 2010-01-29 2011-08-04 Advanced Cell Diagnostics, Inc. Procédés de détection in situ d'acides nucléiques
EP2354242A1 (fr) 2010-02-03 2011-08-10 Epiontis GmbH Analyse pour déterminer le type et/ou le statut d'une cellule en fonction du motif épigénétique et la structure chromatique
US9714446B2 (en) 2010-02-11 2017-07-25 Nanostring Technologies, Inc. Compositions and methods for the detection of small RNAs
EP2536818B1 (fr) 2010-02-18 2018-10-03 Bima Limited Dosage immunomultiplex à billes immobilisées
US10266876B2 (en) 2010-03-08 2019-04-23 California Institute Of Technology Multiplex detection of molecular species in cells by super-resolution imaging and combinatorial labeling
JP5665021B2 (ja) 2010-03-08 2015-02-04 国立大学法人東京農工大学 融合mhc分子連結磁気微粒子、抗原ペプチドのスクリーニング方法、組換えベクター、及び磁性細菌の形質転換体
US10267808B2 (en) 2010-03-08 2019-04-23 California Institute Of Technology Molecular indicia of cellular constituents and resolving the same by super-resolution technologies in single cells
WO2011127099A1 (fr) 2010-04-05 2011-10-13 Prognosys Biosciences, Inc. Tests biologiques à codage spatial
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US20110245101A1 (en) 2010-04-05 2011-10-06 Prognosys Biosciences, Inc. Co-localization affinity assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US8462981B2 (en) 2010-04-07 2013-06-11 Cambridge Research & Instrumentation, Inc. Spectral unmixing for visualization of samples
US10240194B2 (en) 2010-05-13 2019-03-26 Gen9, Inc. Methods for nucleotide sequencing and high fidelity polynucleotide synthesis
US20130059741A1 (en) 2010-05-13 2013-03-07 Illumina, Inc. Binding assays for markers
US8828688B2 (en) 2010-05-27 2014-09-09 Affymetrix, Inc. Multiplex amplification methods
EP2580351B1 (fr) 2010-06-09 2018-08-29 Keygene N.V. Code-barres de séquences combinatoires pour criblage à haut débit
ES2676183T3 (es) 2010-07-02 2018-07-17 Ventana Medical Systems, Inc. Detección de dianas usando marcas de masa y espectrometría de masas
WO2012012772A2 (fr) 2010-07-22 2012-01-26 The Johns Hopkins University Hydrogels à élution de médicaments destinés à être administrés par cathéter
WO2012019133A1 (fr) 2010-08-05 2012-02-09 Cambridge Research & Instrumentation, Inc. Amélioration de l'évaluation visuelle d'échantillons
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
GB201013767D0 (en) 2010-08-17 2010-09-29 Isis Innovation Identification of ligands and their use
DK2623613T3 (en) 2010-09-21 2016-10-03 Population Genetics Tech Ltd Increasing the reliability of the allele-indications by molecular counting
KR20130113447A (ko) * 2010-09-24 2013-10-15 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 고정된 프라이머들을 이용하여 표적 dna의 직접적인 캡쳐, 증폭 및 서열화
US9110079B2 (en) 2010-09-29 2015-08-18 Biomerieux Method and kit for establishing an in vitro prognosis on a patient exhibiting SIRS
WO2012048341A1 (fr) 2010-10-08 2012-04-12 President And Fellows Of Harvard College Établissement à haut débit d'un code-barres de cellules simples
US10669569B2 (en) 2010-10-15 2020-06-02 Navinci Diagnostics Ab Dynamic range methods
EP3034625B1 (fr) 2010-10-21 2017-10-04 Advanced Cell Diagnostics, Inc. Procédé ultrasensible de détection in situ d'acides nucléiques
CA2815076C (fr) 2010-10-22 2021-01-12 Cold Spring Harbor Laboratory Comptage varietal d'acides nucleiques pour obtenir des informations sur le nombre de copies genomiques
EP2632593B1 (fr) 2010-10-27 2021-09-29 Illumina, Inc. Cellules d'écoulement pour analyse biologique ou chimique
US10024796B2 (en) 2010-10-29 2018-07-17 President And Fellows Of Harvard College Nucleic acid nanostructure barcode probes
WO2012071428A2 (fr) 2010-11-22 2012-05-31 Solulink, Inc. Procédés et/ou utilisation de conjugués d'oligonucléotide pour des essais et des détections
US20140121118A1 (en) 2010-11-23 2014-05-01 Opx Biotechnologies, Inc. Methods, systems and compositions regarding multiplex construction protein amino-acid substitutions and identification of sequence-activity relationships, to provide gene replacement such as with tagged mutant genes, such as via efficient homologous recombination
EP2652155B1 (fr) 2010-12-16 2016-11-16 Gigagen, Inc. Procédés pour l'analyse parallèle massive des acides nucléiques contenus dans des cellules individuelles
CN103370425B (zh) 2010-12-17 2019-03-19 生命技术公司 用于核酸扩增的方法、组合物、系统、仪器和试剂盒
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
EP3564395A1 (fr) 2010-12-30 2019-11-06 Foundation Medicine, Inc. Optimisation d'une analyse multigénique d'échantillons tumoraux
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
CN202390428U (zh) * 2011-01-10 2012-08-22 伊鲁米那股份有限公司 射流器件支架
EA201391074A1 (ru) 2011-01-25 2013-12-30 Олмак Дайэгностикс Лимитед Профили экспрессии генов рака толстой кишки и способы применения
AU2012219132B2 (en) 2011-02-15 2016-05-12 Mats Nilsson Bernitz Method for localized in situ detection of mRNA
EP2689028B1 (fr) 2011-03-23 2017-08-30 Pacific Biosciences Of California, Inc. Isolement de complexes polymérase-acide nucléique et charge sur des substrats
US9260753B2 (en) 2011-03-24 2016-02-16 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
US20120258871A1 (en) 2011-04-08 2012-10-11 Prognosys Biosciences, Inc. Peptide constructs and assay systems
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
AU2012242847B2 (en) 2011-04-15 2017-01-19 The Johns Hopkins University Safe sequencing system
US8946389B2 (en) 2011-04-25 2015-02-03 University Of Washington Compositions and methods for multiplex biomarker profiling
CA2834976C (fr) 2011-05-04 2016-03-15 Htg Molecular Diagnostics, Inc. Perfectionnements apportes a un dosage quantitatif (qnpa) et a un sequencage (qnps) avec protection contre la nuclease
SG194722A1 (en) * 2011-05-09 2013-12-30 Fluidigm Corp Probe based nucleic acid detection
DK2710145T3 (en) 2011-05-17 2016-02-22 Dxterity Diagnostics Inc METHOD AND COMPOSITIONS FOR THE DETECTION OF TARGET NUCLEIC ACIDS
WO2012159089A1 (fr) 2011-05-19 2012-11-22 Sequenom, Inc. Produits et procédés d'identification d'acides nucléiques multiplexés
US9005935B2 (en) 2011-05-23 2015-04-14 Agilent Technologies, Inc. Methods and compositions for DNA fragmentation and tagging by transposases
GB201108678D0 (en) 2011-05-24 2011-07-06 Olink Ab Multiplexed proximity ligation assay
WO2012168003A1 (fr) 2011-06-06 2012-12-13 Biocartis S.A. Lyse sélective des cellules par des tensioactifs ioniques selective lysis of cells by ionic surfactants
JP6121409B2 (ja) 2011-06-17 2017-04-26 ロッシュ ダイアグノスティクス ヘマトロジー インコーポレイテッド 生体試料の組織処理のための溶液
WO2013019960A1 (fr) 2011-08-03 2013-02-07 Bio-Rad Laboratories, Inc. Filtration de petits acides nucléiques à l'aide de cellules perméabilisées
WO2013033271A2 (fr) 2011-08-29 2013-03-07 Derren Barken Méthode permettant d'améliorer le système immunitaire en réaction à une maladie ou une lésion
US10385475B2 (en) 2011-09-12 2019-08-20 Adaptive Biotechnologies Corp. Random array sequencing of low-complexity libraries
SI3623481T1 (sl) 2011-09-23 2022-01-31 Illumina, Inc. Sestavki za sekvenciranje nukleinske kisline
US10501791B2 (en) 2011-10-14 2019-12-10 President And Fellows Of Harvard College Sequencing by structure assembly
EP2771103B1 (fr) 2011-10-28 2017-08-16 Illumina, Inc. Système et procédé de fabrication de micropuces
US8987174B2 (en) 2011-10-28 2015-03-24 Prognosys Biosciences, Inc. Methods for manufacturing molecular arrays
CA2854023A1 (fr) 2011-11-07 2013-05-16 Illumina, Inc. Appareils de sequencage integre et procedes d'utilisation
DK2788499T3 (en) 2011-12-09 2016-03-21 Illumina Inc Enhanced root for polymer tags
US20150031553A1 (en) 2011-12-13 2015-01-29 Sequenta, Inc. Method of measuring immune activation
US11021737B2 (en) 2011-12-22 2021-06-01 President And Fellows Of Harvard College Compositions and methods for analyte detection
ES2953308T3 (es) 2011-12-22 2023-11-10 Harvard College Composiciones y métodos para la detección de analitos
US9803188B2 (en) 2011-12-22 2017-10-31 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids
EP2814986B1 (fr) * 2012-02-14 2017-10-25 Cornell University Méthode de quantification relative d'une séquence d'acides nucléiques, d'une expression ou de modifications de copie, en utilisant des réactions combinées de nucléase, ligature, et polymérase
JP5889665B2 (ja) * 2012-02-14 2016-03-22 シャープ株式会社 静電荷現像用トナー、及びそれを用いる画像形成装置、並びに画像形成方法
US10011871B2 (en) 2012-02-17 2018-07-03 Fred Hutchinson Cancer Research Center Compositions and methods for accurately identifying mutations
NO2694769T3 (fr) 2012-03-06 2018-03-03
SG11201405669XA (en) 2012-03-13 2014-10-30 Swift Biosciences Inc Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase
CA2867293C (fr) 2012-03-13 2020-09-01 Abhijit Ajit PATEL Mesure des variants d'acide nucleique au moyen du sequencage hautement multiplexe, a tres haut debit et a suppression d'erreur
ES2828661T3 (es) 2012-03-20 2021-05-27 Univ Washington Through Its Center For Commercialization Métodos para reducir la tasa de error de la secuenciación de ADN masiva en paralelo mediante el uso de la secuenciación de secuencia consenso bicatenaria
FI2831279T3 (fi) 2012-03-26 2023-05-23 Univ Johns Hopkins Nopea aneuploidian havaitseminen
EP2647426A1 (fr) * 2012-04-03 2013-10-09 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Réplication de molécules d'acide nucléique distribuées avec conservation de leur distribution relative par liaison à base d'hybridation
US9193996B2 (en) 2012-04-03 2015-11-24 Illumina, Inc. Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing
AU2013246050B2 (en) 2012-04-13 2017-03-16 Adaptive Biotechnologies Corp. Detection and quantitation of sample contamination in immune repertoire analysis
WO2013158936A1 (fr) 2012-04-20 2013-10-24 Sequenta, Inc Surveillance de l'évolution des chaînes lourdes d'immunoglobuline dans le cas d'une leucémie lymphoblastique aiguë dans le lymphocyte b
US8900529B2 (en) * 2012-04-27 2014-12-02 General Electric Company Microfluidic chamber device and fabrication
EP2659977B1 (fr) 2012-05-02 2019-04-24 IMEC vzw Système microfluidique pour le séquençage
AU2013266419B2 (en) 2012-05-22 2018-09-27 British Columbia Cancer Agency Branch NANO46 genes and methods to predict breast cancer outcome
WO2013184754A2 (fr) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Séquençage spatial d'acides nucléiques à l'aide de sondes d'origami d'adn
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
US20150219618A1 (en) 2012-07-18 2015-08-06 Biological Dynamics, Inc. Manipulation of microparticles in low field dielectrophoretic regions
JP5997278B2 (ja) 2012-07-30 2016-09-28 株式会社日立製作所 タグ配列付き2次元cDNAライブラリーデバイス、並びにこれを用いた遺伝子発現解析方法及び遺伝子発現解析装置
US10545075B2 (en) 2012-08-09 2020-01-28 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for preparing biological specimens for microscopic analysis
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20140378345A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
AU2013302756C1 (en) 2012-08-14 2018-05-17 10X Genomics, Inc. Microcapsule compositions and methods
WO2014031735A1 (fr) 2012-08-21 2014-02-27 Cambridge Research & Instrumentation, Inc. Visualisation et mesure de compartiments cellulaires
WO2014044724A1 (fr) 2012-09-18 2014-03-27 Qiagen Gmbh Procédé et trousse de préparation d'un échantillon cible appauvri en arn
US9732390B2 (en) 2012-09-20 2017-08-15 The Chinese University Of Hong Kong Non-invasive determination of methylome of fetus or tumor from plasma
ES2660027T3 (es) 2012-10-01 2018-03-20 Adaptive Biotechnologies Corporation Evaluación de la inmunocompetencia por la diversidad de los receptores de inmunidad adaptativa y caracterización de la clonalidad
US9783841B2 (en) 2012-10-04 2017-10-10 The Board Of Trustees Of The Leland Stanford Junior University Detection of target nucleic acids in a cellular sample
US9518980B2 (en) 2012-10-10 2016-12-13 Howard Hughes Medical Institute Genetically encoded calcium indicators
EP3511423B1 (fr) * 2012-10-17 2021-03-24 Spatial Transcriptomics AB Procédés et produits pour optimiser la détection localisée ou spatiale de l'expression génique dans un échantillon de tissu
EP2722105A1 (fr) 2012-10-22 2014-04-23 Universität Wien Procédé de synthèse in situ de microréseaux
US10174366B2 (en) 2012-11-14 2019-01-08 Olink Bioscience Ab Localised RCA-based amplification method
EP2925822A4 (fr) 2012-11-27 2016-10-12 Univ Tufts Encres à base de biopolymères et leur utilisation
CN117845337A (zh) 2012-12-10 2024-04-09 分析生物科学有限公司 靶向基因组分析的方法
WO2014110027A1 (fr) 2013-01-10 2014-07-17 Caliper Life Sciences, Inc. Système et procédés d'imagerie multispectrale plein champ
EP2943932B1 (fr) 2013-01-10 2019-09-11 Akoya Biosciences, Inc. Systèmes et procédés d'imagerie multispectrale plein champ
US9758828B2 (en) 2013-01-31 2017-09-12 Cornell University Methods to detect, treat and prevent acute cellular rejection in kidney allografts
KR102190198B1 (ko) 2013-02-08 2020-12-14 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
WO2014130576A1 (fr) 2013-02-19 2014-08-28 Biodot, Inc. Analyse fish automatique d'échantillons de tissus et de cellules à l'aide d'une barrière d'isolation pour la distribution précise d'une sonde et d'autres réactifs sur des régions d'intérêt
CA2901641C (fr) 2013-02-25 2021-02-09 Biocartis N.V. Isolement d'acides nucleiques
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
US10138509B2 (en) 2013-03-12 2018-11-27 President And Fellows Of Harvard College Method for generating a three-dimensional nucleic acid containing matrix
CA2898453C (fr) 2013-03-13 2021-07-27 Illumina, Inc. Dispositifs fluidiques multicouches et leurs procedes de fabrication
WO2014152397A2 (fr) 2013-03-14 2014-09-25 The Broad Institute, Inc. Purification sélective d'arn et de complexes moléculaires liés à l'arn
US9273349B2 (en) 2013-03-14 2016-03-01 Affymetrix, Inc. Detection of nucleic acids
EP3415626B1 (fr) 2013-03-15 2020-01-22 Lineage Biosciences, Inc. Procédés et compositions d'étiquetage et d'analyse d'échantillons
US10656149B2 (en) 2013-03-15 2020-05-19 The Trustees Of Princeton University Analyte detection enhancement by targeted immobilization, surface amplification, and pixelated reading and analysis
US9330295B2 (en) 2013-03-15 2016-05-03 Brown University Spatial sequencing/gene expression camera
WO2014149629A1 (fr) 2013-03-15 2014-09-25 Htg Molecular Diagnostics, Inc. Sous-typage des cancers du poumon
US11441196B2 (en) 2013-03-15 2022-09-13 The Broad Institute, Inc. Ribosomal ribonucleic acid hybridization for organism identification
EP2971152B1 (fr) 2013-03-15 2018-08-01 The Board Of Trustees Of The Leland Stanford Junior University Identification et utilisation de marqueurs tumoraux acides nucléiques circulants
US8808989B1 (en) 2013-04-02 2014-08-19 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
WO2014176435A2 (fr) 2013-04-25 2014-10-30 Bergo Vladislav B Compositions de microréseaux et leurs procédés d'utilisation
US10510435B2 (en) 2013-04-30 2019-12-17 California Institute Of Technology Error correction of multiplex imaging analysis by sequential hybridization
US20160369329A1 (en) 2013-04-30 2016-12-22 California Institute Of Technology Multiplex labeling of molecules by sequential hybridization barcoding using probes with cleavable linkers
JP6605452B2 (ja) 2013-04-30 2019-11-13 カリフォルニア インスティチュート オブ テクノロジー 逐次ハイブリダイゼーションバーコーディングによる分子の多重標識化
EP4321628A3 (fr) 2013-05-23 2024-04-24 The Board of Trustees of the Leland Stanford Junior University Transposition en chromatine native pour l'épigénomique personnelle
WO2014200767A1 (fr) 2013-06-12 2014-12-18 The General Hospital Corporation Procédés, nécessaires et systèmes pour détection multiplexe de molécules cibles et leurs utilisations
WO2014210225A1 (fr) 2013-06-25 2014-12-31 Prognosys Biosciences, Inc. Procédés et systèmes pour déterminer des motifs spatiales de cibles biologiques dans un échantillon
US20150000854A1 (en) 2013-06-27 2015-01-01 The Procter & Gamble Company Sheet products bearing designs that vary among successive sheets, and apparatus and methods for producing the same
ES2700529T3 (es) 2013-07-01 2019-02-18 Illumina Inc Funcionalización de superficie sin catalizador e injerto de polímero
AU2014312208B2 (en) 2013-08-28 2019-07-25 Becton, Dickinson And Company Massively parallel single cell analysis
EP3570037A1 (fr) 2013-09-13 2019-11-20 The Board of Trustees of the Leland Stanford Junior University Imagerie multiplexée de tissus à l'aide d'étiquettes de masse et procédé de spectrométrie de masse ionique secondaire
JP2016538569A (ja) 2013-09-20 2016-12-08 カリフォルニア インスティチュート オブ テクノロジー 無傷全組織の表現型分類のための方法
US10794802B2 (en) 2013-09-20 2020-10-06 California Institute Of Technology Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high resolution intact circuit mapping and phenotyping
WO2015054292A1 (fr) 2013-10-07 2015-04-16 Cellular Research, Inc. Procédés et systèmes de comptage numérique de zones caractéristiques sur des jeux ordonnés d'échantillons
US10540783B2 (en) 2013-11-01 2020-01-21 Illumina, Inc. Image analysis useful for patterned objects
WO2015069374A1 (fr) 2013-11-07 2015-05-14 Agilent Technologies, Inc. Pluralité d'adaptateurs de transposase destinés à des manipulations d'adn
US9834814B2 (en) 2013-11-22 2017-12-05 Agilent Technologies, Inc. Spatial molecular barcoding of in situ nucleic acids
GB2520765A (en) 2013-12-02 2015-06-03 Vanadis Diagnostics Ab Multiplex detection of nucleic acids
CN106460032B (zh) 2013-12-05 2019-12-24 生捷科技控股公司 图案化阵列的制备
WO2015117163A2 (fr) 2014-02-03 2015-08-06 Integrated Dna Technologies, Inc. Procédés pour capturer et/ou éliminer des arn très abondants dans un échantillon d'arn hétérogène
GB201401885D0 (en) 2014-02-04 2014-03-19 Olink Ab Proximity assay with detection based on hybridisation chain reaction (HCR)
US10309879B2 (en) 2014-02-21 2019-06-04 Massachusetts Institute Of Technology Expansion microscopy
CA2940764C (fr) 2014-02-26 2019-10-29 Nelson Alexander Procede photo-selectif utilisable dans le domaine de l'analyse d'echantillons biologiques
WO2015148606A2 (fr) 2014-03-25 2015-10-01 President And Fellows Of Harvard College Puce à protéines à code-barres pour le profilage d'une interaction monomoléculaire multiplex
DE202015009494U1 (de) 2014-04-10 2018-02-08 10X Genomics, Inc. Fluidische Vorrichtungen und Systeme zur Einkapselung und Partitionierung von Reagenzien, und deren Anwendungen
WO2015154334A1 (fr) 2014-04-11 2015-10-15 珠海美路得企业发展有限公司 Système de détection sur biopuce
AU2015247779B2 (en) 2014-04-15 2021-06-24 Illumina, Inc. Modified transposases for improved insertion sequence bias and increased DNA input tolerance
WO2015161173A1 (fr) 2014-04-18 2015-10-22 William Marsh Rice University Compositions compétitives de molécules d'acides nucléiques destinées à enrichir les espèces porteuses d'allèles rares
AU2015266894A1 (en) 2014-05-30 2016-08-25 The Board Of Trustees Of The Leland Stanford Junior University Methods and devices for imaging large intact tissue samples
US10408842B2 (en) 2014-05-30 2019-09-10 The Regents Of The University Of California Subcellular western blotting of single cells
EP3152232B1 (fr) 2014-06-06 2019-11-13 Herlev Hospital Détermination de reconnaissance d'antigène par l'intermédiaire d'un marquage par code-barres de multimères du cmh
EP3155426B1 (fr) 2014-06-13 2023-07-19 Immudex ApS Détection générale et isolement de cellules spécifiques par liaison de molécules marquées
US9909167B2 (en) 2014-06-23 2018-03-06 The Board Of Trustees Of The Leland Stanford Junior University On-slide staining by primer extension
MX2016016902A (es) 2014-06-26 2017-03-27 10X Genomics Inc Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas.
JP6652512B2 (ja) 2014-06-30 2020-02-26 イラミーナ インコーポレーテッド 片側転移を用いた方法および組成物
EP3167278B1 (fr) 2014-07-09 2019-06-26 Caliper Life Sciences, Inc. Extraction de spectres purs d'échantillons biologiques
US10179932B2 (en) 2014-07-11 2019-01-15 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
WO2016011429A1 (fr) 2014-07-17 2016-01-21 California Institute Of Technology Analyse multiplex de molécules dans des organismes unicellulaires par corrélation d'images
EP3175023A4 (fr) 2014-07-30 2018-03-07 President and Fellows of Harvard College Systèmes et méthodes permettant de déterminer des acides nucléiques
CN107075546B (zh) 2014-08-19 2021-08-31 哈佛学院董事及会员团体 用于对核酸探测并作图的rna-引导的系统
US11091810B2 (en) 2015-01-27 2021-08-17 BioSpyder Technologies, Inc. Focal gene expression profiling of stained FFPE tissues with spatial correlation to morphology
US9957550B2 (en) 2014-09-08 2018-05-01 BioSpyder Technologies, Inc. Attenuators
US9938566B2 (en) 2014-09-08 2018-04-10 BioSpyder Technologies, Inc. Profiling expression at transcriptome scale
US9856521B2 (en) 2015-01-27 2018-01-02 BioSpyder Technologies, Inc. Ligation assays in liquid phase
EP4105337A1 (fr) 2014-09-09 2022-12-21 The Broad Institute, Inc. Procédé et appareil à base de gouttelettes pour l'analyse composite d'acide nucléique de cellules uniques
WO2016044313A1 (fr) 2014-09-16 2016-03-24 The Board Of Trustees Of The Leland Stanford Junior University Procédés et compositions pour l'élimination de produits d'addition et de réticulation d'aldéhyde de molécules biologiques
US20170349940A1 (en) 2014-09-26 2017-12-07 Two Pore Guys, Inc. Targeted Sequence Detection by Nanopore Sensing of Synthetic Probes
US10405750B2 (en) 2014-09-30 2019-09-10 Washington University Nonradiative voltage-sensitive dye for imaging neuronal action potential
US20160108458A1 (en) 2014-10-06 2016-04-21 The Board Of Trustees Of The Leland Stanford Junior University Multiplexed detection and quantification of nucleic acids in single-cells
CN107002128A (zh) 2014-10-29 2017-08-01 10X 基因组学有限公司 用于靶核酸测序的方法和组合物
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
EP3218467A4 (fr) 2014-11-13 2018-04-11 The Board of Trustees of the University of Illinois "super" hélicases hyper-fonctionnelle bio-modifiées
CN107208144B (zh) 2014-11-21 2021-06-08 纳米线科技公司 无酶且无扩增的测序
JP7144142B2 (ja) 2014-11-24 2022-09-29 ナノストリング テクノロジーズ,インコーポレイティド 遺伝子精製および画像化のための方法および装置
CN104513785B (zh) 2014-12-17 2017-07-18 华中科技大学 一种细胞寻址微流控芯片、细胞分析装置及方法
EP3234192B1 (fr) 2014-12-19 2021-07-14 The Broad Institute, Inc. Identification non biaisée de cassures bicaténaires et réarrangement génomique par séquençage de capture d'insert à l'échelle du génome
EP3271480B8 (fr) 2015-01-06 2022-09-28 Molecular Loop Biosciences, Inc. Criblage de variants structuraux
WO2016118915A1 (fr) 2015-01-22 2016-07-28 Becton, Dickinson And Company Dispositifs et systèmes permettant le codage moléculaire à barres d'acides nucléiques cibles dans des cellules individuelles
CA2974306A1 (fr) 2015-02-04 2016-08-11 The Regents Of The University Of California Sequencage d'acides nucleiques contenus dans des entites individuelles par barcoding
CA2975852A1 (fr) 2015-02-04 2016-08-11 Twist Bioscience Corporation Procedes et dispositifs pour assemblage de novo d'acide oligonucleique
CN107208158B (zh) 2015-02-27 2022-01-28 贝克顿迪金森公司 空间上可寻址的分子条形编码
CN107208157B (zh) * 2015-02-27 2022-04-05 贝克顿迪金森公司 用于条形编码核酸以用于测序的方法和组合物
WO2016154302A1 (fr) 2015-03-23 2016-09-29 The University Of North Carolina At Chapel Hill Processeur moléculaire universel pour la médecine de précision
EP3835431B1 (fr) 2015-03-30 2022-11-02 Becton, Dickinson and Company Procédés pour codage à barres combinatoire
US20180095067A1 (en) 2015-04-03 2018-04-05 Abbott Laboratories Devices and methods for sample analysis
CN112892628B (zh) 2015-04-03 2023-08-15 雅培制药有限公司 用于样品分析的装置和方法
EP3530752B1 (fr) 2015-04-10 2021-03-24 Spatial Transcriptomics AB Analyse de plusieurs acides nucléiques spatialement différenciés de spécimens biologiques
RU2733545C2 (ru) 2015-04-14 2020-10-05 Конинклейке Филипс Н.В. Пространственное картирование молекулярных профилей образцов биологических тканей
US10526649B2 (en) 2015-04-14 2020-01-07 Massachusetts Institute Of Technology Augmenting in situ nucleic acid sequencing of expanded biological samples with in vitro sequence information
US11408890B2 (en) 2015-04-14 2022-08-09 Massachusetts Institute Of Technology Iterative expansion microscopy
US10059990B2 (en) 2015-04-14 2018-08-28 Massachusetts Institute Of Technology In situ nucleic acid sequencing of expanded biological samples
US20180057873A1 (en) 2015-04-17 2018-03-01 Centrillion Technology Holdings Corporation Methods for performing spatial profiling of biological materials
CA3184363C (fr) 2015-04-21 2024-01-02 Isolation Bio Inc. Systemes a haute resolution, trousses, appareil, et procedes pour applications de microbiologie a rendement eleve
US10767220B2 (en) 2015-05-21 2020-09-08 Becton, Dickinson And Company Methods of amplifying nucleic acids and compositions for practicing the same
WO2017015099A1 (fr) 2015-07-17 2017-01-26 Nanostring Technologies, Inc. Quantification simultanée de l'expression génique dans une région définie par l'utilisateur d'un tissu en coupe transversale
CA2992480A1 (fr) 2015-07-17 2017-01-26 Nanostring Technologies, Inc. Quantification simultanee d'une pluralite de proteines dans une region definie par l'utilisateur d'un tissu en coupe transversale
ES2959190T3 (es) 2015-07-24 2024-02-21 Univ Johns Hopkins Composiciones y métodos de análisis de ARN
ES2888626T3 (es) 2015-07-27 2022-01-05 Illumina Inc Cartografiado espacial de información de secuencia de ácidos nucleicos
WO2017027367A1 (fr) 2015-08-07 2017-02-16 Massachusetts Institute Of Technology Imagerie à l'échelle nanométrique de protéines et d'acides nucléiques par microscopie d'expansion
US10317321B2 (en) 2015-08-07 2019-06-11 Massachusetts Institute Of Technology Protein retention expansion microscopy
WO2017027549A1 (fr) 2015-08-10 2017-02-16 Duke University Réseaux monocellulaires magnétiques pour la vérification de communication cellule-cellule et cellule-médicament
US11118216B2 (en) 2015-09-08 2021-09-14 Affymetrix, Inc. Nucleic acid analysis by joining barcoded polynucleotide probes
WO2017044614A1 (fr) 2015-09-11 2017-03-16 The Regents Of The University Of California Réseaux de focalisation isoélectrique et leurs procédés d'utilisation
MY194887A (en) 2015-09-14 2022-12-21 Essenlix Corp Device and System for Analyzing a Sample, Particularly Blood, as Well as Methods of Using The Same
US11214661B2 (en) 2015-09-17 2022-01-04 Massachusetts Institute Of Technology Three-dimensional nanofabrication by patterning of hydrogels
ES2893100T3 (es) 2015-10-12 2022-02-08 Advanced Cell Diagnostics Inc Detección in situ de variantes de nucleótidos en muestras con un elevado nivel de ruido, y composiciones y métodos relacionados con ésta
WO2017075293A1 (fr) 2015-10-28 2017-05-04 Silicon Valley Scientific, Inc. Procédé et appareil pour encoder des informations de position spatiale cellulaire
WO2017075294A1 (fr) 2015-10-28 2017-05-04 The Board Institute Inc. Dosages utilisés pour le profilage de perturbation massivement combinatoire et la reconstruction de circuit cellulaire
US11092607B2 (en) 2015-10-28 2021-08-17 The Board Institute, Inc. Multiplex analysis of single cell constituents
US10802262B2 (en) 2015-10-29 2020-10-13 The Board Of Trustees Of The Leland Stanford Junior University Methods and systems for imaging a biological sample
JP6882282B2 (ja) 2015-11-03 2021-06-02 プレジデント アンド フェローズ オブ ハーバード カレッジ 三次元核酸含有マトリックスの立体撮像のための方法と装置
US20190155835A1 (en) 2015-11-03 2019-05-23 President And Fellows Of Harvard College Systems and Methods for Processing Spatially Related Sequence Data Received from a Sequencing Device
IL290309B2 (en) 2015-11-06 2024-04-01 Ventana Med Syst Inc Representative diagnoses
DK3882357T3 (da) 2015-12-04 2022-08-29 10X Genomics Inc Fremgangsmåder og sammensætninger til analyse af nukleinsyrer
US10281369B2 (en) 2015-12-23 2019-05-07 VOR, Inc. Dual-image based bioimaging devices and techniques
US10049770B2 (en) 2015-12-30 2018-08-14 Case Western Reserve University Prediction of recurrence of non-small cell lung cancer
US20190144936A1 (en) 2016-01-15 2019-05-16 Massachusetts Institute Of Technology Semi-permeable arrays for analyzing biological systems and methods of using same
US11254974B2 (en) 2016-02-10 2022-02-22 The Board Of Trustees Of The Leland Stanford Junior University RNA fixation and detection in clarity-based hydrogel tissue
US10633648B2 (en) 2016-02-12 2020-04-28 University Of Washington Combinatorial photo-controlled spatial sequencing and labeling
US20170241911A1 (en) 2016-02-22 2017-08-24 Miltenyi Biotec Gmbh Automated analysis tool for biological specimens
US11360025B2 (en) 2016-02-22 2022-06-14 Miltenyi Biotec B.V. & Co. KG Automated analysis tool for biological specimens
EP4015647B1 (fr) 2016-02-26 2023-08-30 The Board of Trustees of the Leland Stanford Junior University Visualisation de molécule simple multiplexée d'arn à l'aide d'un système de ligature de proximité à deux sondes
US20170253918A1 (en) 2016-03-01 2017-09-07 Expansion Technologies Combining protein barcoding with expansion microscopy for in-situ, spatially-resolved proteomics
SG11201807444PA (en) 2016-03-10 2018-09-27 Univ Leland Stanford Junior Transposase-mediated imaging of the accessible genome
WO2017156521A1 (fr) 2016-03-11 2017-09-14 The Johns Hopkins University Procédé et appareil de fabrication de fibres électrofilées alignées ou torsadées et dispositifs
US10844426B2 (en) 2016-03-17 2020-11-24 President And Fellows Of Harvard College Methods for detecting and identifying genomic nucleic acids
WO2017164936A1 (fr) 2016-03-21 2017-09-28 The Broad Institute, Inc. Procédés de détermination de la dynamique d'expression génique spatiale et temporelle dans des cellules uniques
WO2017172860A1 (fr) 2016-03-31 2017-10-05 President And Fellows Of Harvard College Procédés et compositions pour la préparation en tube unique de bibliothèques de séquençage à l'aide de cas9
CA3020814A1 (fr) 2016-04-15 2017-10-19 University Health Network Sequencage de capture hybride pour determiner la clonalite de cellules immunitaires
US20190135774A1 (en) 2016-04-21 2019-05-09 Cell Data Sciences, Inc. Biomolecule processing from fixed biological samples
CN109415761B (zh) 2016-04-25 2022-09-20 哈佛学院董事及会员团体 用于原位分子检测的杂交链反应方法
KR20230047506A (ko) 2016-05-02 2023-04-07 엔코디아, 인코포레이티드 암호화 핵산을 사용한 거대분자 분석
US20180052081A1 (en) 2016-05-11 2018-02-22 Expansion Technologies Combining modified antibodies with expansion microscopy for in-situ, spatially-resolved proteomics
EP4324929A1 (fr) 2016-05-16 2024-02-21 Nanostring Technologies, Inc. Procédés pour détecter des acides nucléiques dans un échantillon
US10894990B2 (en) 2016-05-17 2021-01-19 Shoreline Biome, Llc High throughput method for identification and sequencing of unknown microbial and eukaryotic genomes from complex mixtures
US10495554B2 (en) 2016-05-25 2019-12-03 The Board Of Trustees Of The Leland Stanford Junior University Method and system for imaging and analysis of a biological specimen
EP3472359B1 (fr) 2016-06-21 2022-03-16 10X Genomics, Inc. Séquençage d'acide nucléique
US10450599B2 (en) 2016-07-05 2019-10-22 California Institute Of Technology Fractional initiator hybridization chain reaction
EP3490616B1 (fr) 2016-07-27 2022-09-28 The Board of Trustees of the Leland Stanford Junior University Imagerie fluorescente hautement multiplexée
WO2018023068A1 (fr) 2016-07-29 2018-02-01 New England Biolabs, Inc. Procédés et compositions destinés à prévenir la concatémérisation pendant la commutation de matrice
US20210017587A1 (en) 2016-08-01 2021-01-21 California Institute Of Technology Sequential probing of molecular targets based on pseudo-color barcodes with embedded error correction mechanism
CA3034617A1 (fr) 2016-08-30 2018-03-08 California Institute Of Technology Immunohistochimie par reaction en chaine d'hybridation
CN109923216A (zh) 2016-08-31 2019-06-21 哈佛学院董事及会员团体 将生物分子的检测组合到使用荧光原位测序的单个试验的方法
CN109983125A (zh) 2016-08-31 2019-07-05 哈佛学院董事及会员团体 生成用于通过荧光原位测序检测的核酸序列文库的方法
US11505819B2 (en) 2016-09-22 2022-11-22 William Marsh Rice University Molecular hybridization probes for complex sequence capture and analysis
CN110114520B (zh) 2016-10-01 2023-08-08 伯克利之光生命科技公司 Dna条形码组合物和在微流体装置中原位识别的方法
JP2018062131A (ja) 2016-10-13 2018-04-19 コニカミノルタ株式会社 光書き込み装置及び画像形成装置
WO2018075436A1 (fr) 2016-10-17 2018-04-26 West Jason A A Analyse génomique spatiale haute résolution de tissus et d'agrégats de cellules
CN114875125A (zh) 2016-10-19 2022-08-09 10X基因组学有限公司 用于条形码化单个细胞或细胞群的核酸分子的方法和系统
US10515450B2 (en) 2016-10-20 2019-12-24 University Of Florida Research Foundation, Inc. Systems and methods for using a single-cell to create chromosomal spreads
WO2018085599A2 (fr) 2016-11-02 2018-05-11 ArcherDX, Inc. Procédés de préparation d'échantillon d'acide nucléique pour le séquençage de répertoire immunitaire
WO2018089445A1 (fr) 2016-11-08 2018-05-17 President And Fellows Of Harvard College Impression et élimination de matrices
WO2018089438A1 (fr) 2016-11-08 2018-05-17 President And Fellows Of Harvard College Imagerie multiplexée utilisant la technique merfish, la microscopie à expansion et les technologies associées
GB201619458D0 (en) 2016-11-17 2017-01-04 Spatial Transcriptomics Ab Method for spatial tagging and analysing nucleic acids in a biological specimen
CN116334202A (zh) 2016-11-21 2023-06-27 纳米线科技公司 化学组合物及其使用方法
US11385236B2 (en) 2016-11-30 2022-07-12 Howard Hughes Medical Institute Chemigenetic voltage indicators
US10656144B2 (en) 2016-12-02 2020-05-19 The Charlotte Mecklenburg Hospital Authority Immune profiling and minimal residue disease following stem cell transplantation in multiple myeloma
WO2018107054A1 (fr) 2016-12-09 2018-06-14 Ultivue, Inc. Procédés améliorés d'imagerie multiplex utilisant des agents d'imagerie d'acides nucléiques marqués
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20190177800A1 (en) 2017-12-08 2019-06-13 10X Genomics, Inc. Methods and compositions for labeling cells
US10711269B2 (en) 2017-01-18 2020-07-14 Agilent Technologies, Inc. Method for making an asymmetrically-tagged sequencing library
WO2018136856A1 (fr) 2017-01-23 2018-07-26 Massachusetts Institute Of Technology Hybridation in situ en fluorescence par multiplexage à signal amplifié par l'intermédiaire d'une amplification et d'un séquençage de ligature par épissage
EP4029939B1 (fr) 2017-01-30 2023-06-28 10X Genomics, Inc. Procédés et systèmes de codage à barres de cellules individuelles sur la base de gouttelettes
GB201701691D0 (en) 2017-02-01 2017-03-15 Illumina Inc System and method with reflective fiducials
EP4324931A2 (fr) 2017-02-02 2024-02-21 New York Genome Center, Inc. Procédés et compositions pour identifier ou quantifier des cibles dans un échantillon biologique
CN116794819A (zh) 2017-02-08 2023-09-22 Essenlix 公司 用于测定的光学器件、装置和系统
US11723907B2 (en) 2017-02-23 2023-08-15 The Cleveland Clinic Foundation Anti-aging compounds
JP7248368B2 (ja) 2017-03-01 2023-03-29 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 高度に特異的な環状近接ライゲーションアッセイ
US10549279B2 (en) 2017-08-22 2020-02-04 10X Genomics, Inc. Devices having a plurality of droplet formation regions
CN111263819A (zh) 2017-10-06 2020-06-09 卡特阿纳公司 Rna模板化连接
US11753676B2 (en) 2017-10-11 2023-09-12 Expansion Technologies Multiplexed in situ hybridization of tissue sections for spatially resolved transcriptomics with expansion microscopy
WO2019157529A1 (fr) 2018-02-12 2019-08-15 10X Genomics, Inc. Procédés de caractérisation d'analytes multiples à partir de cellules individuelles ou de populations cellulaires
EP3775271A1 (fr) 2018-04-06 2021-02-17 10X Genomics, Inc. Systèmes et procédés de contrôle de qualité dans un traitement de cellules uniques
SG11202102029TA (en) 2018-08-28 2021-03-30 10X Genomics Inc Methods for generating spatially barcoded arrays
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
US20210317524A1 (en) 2018-08-28 2021-10-14 10X Genomics, Inc. Resolving spatial arrays
US20210324457A1 (en) 2018-08-28 2021-10-21 Eswar Prasad Ramachandran Iyer Methods for Generating Spatially Barcoded Arrays
TWI816881B (zh) 2018-09-13 2023-10-01 大陸商恒翼生物醫藥(上海)股份有限公司 用於治療三陰性乳癌之組合療法
WO2020061064A1 (fr) 2018-09-17 2020-03-26 Piggy Llc Systèmes, procédés et programmes informatiques destinés à procurer à des utilisateurs un maximum de profit dans le commerce électronique
US11694779B2 (en) 2018-09-17 2023-07-04 Labsavvy Health, Llc Systems and methods for automated reporting and education for laboratory test results
EP3834401B1 (fr) 2018-09-17 2023-04-05 Schneider Electric Systems USA, Inc. Détection d'événement de système industriel et réponse correspondante
WO2020076979A1 (fr) 2018-10-10 2020-04-16 Readcoor, Inc. Capture de surface de cibles
US20220049293A1 (en) 2018-12-10 2022-02-17 10X Genomics, Inc. Methods for determining a location of a biological analyte in a biological sample
EP3894587A1 (fr) 2018-12-10 2021-10-20 10X Genomics, Inc. Résolution de réseaux spatiaux par déconvolution basée sur la proximité
US20210189475A1 (en) 2018-12-10 2021-06-24 10X Genomics, Inc. Imaging system hardware
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US20220267844A1 (en) 2019-11-27 2022-08-25 10X Genomics, Inc. Methods for determining a location of a biological analyte in a biological sample
WO2020167862A1 (fr) 2019-02-12 2020-08-20 10X Genomics, Inc. Systèmes et procédés pour le transfert de réactifs entre des gouttelettes
WO2020176882A1 (fr) 2019-02-28 2020-09-03 10X Genomics, Inc. Dispositifs, systèmes et procédés pour augmenter l'efficacité de formation de gouttelettes
US20230159995A1 (en) 2019-02-28 2023-05-25 10X Genomics, Inc. Profiling of biological analytes with spatially barcoded oligonucleotide arrays
EP3931354A1 (fr) 2019-02-28 2022-01-05 10X Genomics, Inc. Profilage d'analytes biologiques avec des réseaux d'oligonucléotides à codes-barres spatiaux
US11554031B2 (en) 2019-03-01 2023-01-17 Anclote Manufacturing Company, Inc. Attachment plate for a prosthetic socket
EP3938538A1 (fr) 2019-03-15 2022-01-19 10X Genomics, Inc. Méthodes d'utilisation de matrices spatiales pour le séquençage d'organismes unicellulaires
US20220145361A1 (en) 2019-03-15 2022-05-12 10X Genomics, Inc. Methods for using spatial arrays for single cell sequencing
US20220017951A1 (en) 2019-03-22 2022-01-20 10X Genomics, Inc. Three-dimensional spatial analysis
WO2020198071A1 (fr) 2019-03-22 2020-10-01 10X Genomics, Inc. Analyse spatiale tridimensionnelle
WO2020219901A1 (fr) 2019-04-26 2020-10-29 10X Genomics, Inc. Dispositifs de support en imagerie
WO2020243579A1 (fr) 2019-05-30 2020-12-03 10X Genomics, Inc. Procédés de détection de l'hétérogénéité spatiale d'un échantillon biologique
US20210140982A1 (en) 2019-10-18 2021-05-13 10X Genomics, Inc. Identification of spatial biomarkers of brain disorders and methods of using the same
WO2021091611A1 (fr) 2019-11-08 2021-05-14 10X Genomics, Inc. Agents de capture d'analytes marqués spatialement pour le multiplexage d'analytes
EP4025711A2 (fr) 2019-11-08 2022-07-13 10X Genomics, Inc. Amélioration de la spécificité de la liaison d'un analyte
US20230002812A1 (en) 2019-11-13 2023-01-05 10X Genomics, Inc. Generating capture probes for spatial analysis
US20210199660A1 (en) 2019-11-22 2021-07-01 10X Genomics, Inc. Biomarkers of breast cancer
WO2021133842A1 (fr) 2019-12-23 2021-07-01 10X Genomics, Inc. Compositions et méthodes d'utilisation d'échantillons biologiques fixés dans des dosages basés sur des compartiments
WO2021133845A1 (fr) 2019-12-23 2021-07-01 10X Genomics, Inc. Réactifs de fixation réversibles et leurs méthodes d'utilisation
EP3891300B1 (fr) 2019-12-23 2023-03-29 10X Genomics, Inc. Procédés d'analyse spatiale utilisant une ligature à matrice d'arn
US20210198741A1 (en) 2019-12-30 2021-07-01 10X Genomics, Inc. Identification of spatial biomarkers of heart disorders and methods of using the same
US20220348992A1 (en) 2020-01-10 2022-11-03 10X Genomics, Inc. Methods for determining a location of a target nucleic acid in a biological sample
CN115715329A (zh) 2020-01-10 2023-02-24 10X基因组学有限公司 确定生物样品中靶核酸位置的方法
US20210214785A1 (en) 2020-01-13 2021-07-15 Spatial Transcriptomics Ab Methods of decreasing background on a spatial array
US20210223227A1 (en) 2020-01-17 2021-07-22 Spatial Transcriptomics Ab Electrophoretic system and method for analyte capture
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210222253A1 (en) 2020-01-21 2021-07-22 10X Genomics, Inc. Identification of biomarkers of glioblastoma and methods of using the same
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US20210237022A1 (en) 2020-01-31 2021-08-05 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US20210238664A1 (en) 2020-02-03 2021-08-05 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US20230047782A1 (en) 2020-02-07 2023-02-16 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
WO2021168278A1 (fr) 2020-02-20 2021-08-26 10X Genomics, Inc. Procédés pour combiner les synthèses d'adnc des premier et de deuxième brins pour l'analyse spatiale
AU2021224760A1 (en) 2020-02-21 2022-09-15 10X Genomics, Inc. Capturing genetic targets using a hybridization approach
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
WO2021207610A1 (fr) 2020-04-10 2021-10-14 10X Genomics, Inc. Procédé de traitement de protéase à basse température pour la préparation d'échantillons biologiques
WO2021216708A1 (fr) 2020-04-22 2021-10-28 10X Genomics, Inc. Procédés d'analyse spatiale utilisant un appauvrissement d'arn ciblée
EP4146819A1 (fr) 2020-05-04 2023-03-15 10X Genomics, Inc. Modes de transfert transcriptomique spatial
US20230194469A1 (en) 2020-05-19 2023-06-22 10X Genomics, Inc. Electrophoresis cassettes and instrumentation
WO2021237056A1 (fr) 2020-05-22 2021-11-25 10X Genomics, Inc. Analyse d'intégrité d'arn dans un échantillon biologique
WO2021236929A1 (fr) 2020-05-22 2021-11-25 10X Genomics, Inc. Mesure spatio-temporelle simultanée de l'expression génique et de l'activité cellulaire
WO2021237087A1 (fr) 2020-05-22 2021-11-25 10X Genomics, Inc. Analyse spatiale pour détecter des variants de séquence
WO2021242834A1 (fr) 2020-05-26 2021-12-02 10X Genomics, Inc. Procédé de réinitialisation d'un réseau
WO2021247568A1 (fr) 2020-06-02 2021-12-09 10X Genomics, Inc. Trancriptomique spatiale pour les récepteurs d'antigènes
AU2021283174A1 (en) 2020-06-02 2023-01-05 10X Genomics, Inc. Nucleic acid library methods
EP4162074B1 (fr) 2020-06-08 2024-04-24 10X Genomics, Inc. Méthodes de détermination de marge chirurgicale et méthodes d'utilisation associées
US20230279474A1 (en) 2020-06-10 2023-09-07 10X Genomics, Inc. Methods for spatial analysis using blocker oligonucleotides
WO2021252591A1 (fr) 2020-06-10 2021-12-16 10X Genomics, Inc. Procédés de détermination d'un emplacement d'un analyte dans un échantillon biologique
CN116034166A (zh) 2020-06-25 2023-04-28 10X基因组学有限公司 Dna甲基化的空间分析
CN116323968A (zh) 2020-07-31 2023-06-23 10X基因组学有限公司 用于空间分析的去交联化合物和使用方法
EP4200441A1 (fr) 2020-09-15 2023-06-28 10X Genomics, Inc. Procédés de libération d'une sonde de capture étendue à partir d'un substrat et leurs utilisations
AU2021345133A1 (en) 2020-09-16 2023-03-30 10X Genomics, Inc. Methods of determining the location of an analyte in a biological sample using a plurality of wells
WO2022087273A1 (fr) 2020-10-22 2022-04-28 10X Genomics, Inc. Procédés d'analyse spatiale utilisant l'amplification par cercle roulant
WO2022098810A1 (fr) 2020-11-06 2022-05-12 10X Genomics, Inc. Dispositifs de support de dosage
EP4222283A1 (fr) 2020-11-06 2023-08-09 10X Genomics, Inc. Compositions et procédés de liaison d'un analyte à une sonde de capture
WO2022103712A1 (fr) 2020-11-13 2022-05-19 10X Genomics, Inc. Nano-séparations (enzymes de traitement d'acides nucléiques encapsulées) pour la lyse cellulaire et réactions multiples dans des dosages basés sur la séparation
AU2021385065A1 (en) 2020-11-18 2023-06-15 10X Genomics, Inc. Methods and compositions for analyzing immune infiltration in cancer stroma to predict clinical outcome
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US20240068017A1 (en) 2020-12-30 2024-02-29 10X Genomics, Inc. Methods for analyte capture determination
WO2022147296A1 (fr) 2020-12-30 2022-07-07 10X Genomics, Inc. Clivage de sondes de capture pour analyse spatiale
US20240093290A1 (en) 2021-01-29 2024-03-21 10X Genomics, Inc. Method for transposase mediated spatial tagging and analyzing genomic dna in a biological sample
EP4294571A2 (fr) 2021-02-19 2023-12-27 10X Genomics, Inc. Dispositifs de support de dosage modulaires
EP4301870A1 (fr) 2021-03-18 2024-01-10 10X Genomics, Inc. Capture multiplex de gène et expression de protéines à partir d'un échantillon biologique
EP4305196A1 (fr) 2021-04-14 2024-01-17 10X Genomics, Inc. Procédés de mesure d'une mauvaise localisation d'un analyte
WO2022226057A1 (fr) 2021-04-20 2022-10-27 10X Genomics, Inc. Procédés d'évaluation de la qualité d'un échantillon avant une analyse spatiale à l'aide d'une ligature modélisée
US20220333192A1 (en) 2021-04-20 2022-10-20 10X Genomics, Inc. Methods and devices for spatial assessment of rna quality
EP4320271A1 (fr) 2021-05-06 2024-02-14 10X Genomics, Inc. Procédés pour augmenter la résolution d'une analyse spatiale
WO2022256503A1 (fr) 2021-06-03 2022-12-08 10X Genomics, Inc. Procédés, compositions, kits et systèmes pour améliorer la capture d'analytes pour une analyse spatiale
WO2022271820A1 (fr) 2021-06-22 2022-12-29 10X Genomics, Inc. Détection spatiale de sars-cov-2 à l'aide d'une ligature à matrice
US20230014008A1 (en) 2021-07-13 2023-01-19 10X Genomics, Inc. Methods for improving spatial performance
WO2023287765A1 (fr) 2021-07-13 2023-01-19 10X Genomics, Inc. Procédés d'analyse spatiale utilisant un silençage de sonde ciblée
US20230034216A1 (en) 2021-07-28 2023-02-02 10X Genomics, Inc. Multiplexed spatial capture of analytes
US20230034039A1 (en) 2021-08-02 2023-02-02 10X Genomics, Inc. Methods of preserving a biological sample
US20230042817A1 (en) 2021-08-04 2023-02-09 10X Genomics, Inc. Analyte capture from an embedded biological sample
WO2023018799A1 (fr) 2021-08-12 2023-02-16 10X Genomics, Inc. Procédés, compositions et systèmes pour identifier des molécules de liaison à l'antigène
EP4196605A1 (fr) 2021-09-01 2023-06-21 10X Genomics, Inc. Procédés, compositions et kits pour bloquer une sonde de capture sur un réseau spatial
US20230135010A1 (en) 2021-11-03 2023-05-04 10X Genomics, Inc. Sequential analyte capture
WO2023086880A1 (fr) 2021-11-10 2023-05-19 10X Genomics, Inc. Procédés, compositions et kits pour déterminer l'emplacement d'un analyte dans un échantillon biologique

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