KR900014594A - 핵산 분리방법 - Google Patents

핵산 분리방법 Download PDF

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KR900014594A
KR900014594A KR1019900004012A KR900004012A KR900014594A KR 900014594 A KR900014594 A KR 900014594A KR 1019900004012 A KR1019900004012 A KR 1019900004012A KR 900004012 A KR900004012 A KR 900004012A KR 900014594 A KR900014594 A KR 900014594A
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nucleic acid
solid
complex
silica particles
washed
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KR1019900004012A
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KR0148693B1 (ko
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마리아 알레이다 헨리에트
레느 부움 빌렘
키에비츠 팀
프랭클린 렌스 피터
Original Assignee
에프.쥐.엠.헤르만스, 에이.쥐.제이.베르메렌
악조 엔.브이.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

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  • Chemical & Material Sciences (AREA)
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  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
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  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Hard Magnetic Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

내용 없음

Description

핵산 분리방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (15)

  1. 출발물질, 카오트로픽 물질 및 핵산 결합 고상을 혼합하고, 자체에 핵산이 결합된 고상을 액으로부터 분리한 후, 수득된 고상-핵산 복합체를 세정하고, 이어서 필요하다면 상기 복합체로부터 핵산을 용리시키는 것을 특징으로 하는 핵산-함유 출발물질로부터 핵산을 분리하는 방법.
  2. 제1항에 있어서, 사용하는 출발물질이 전혈, 혈청, 연막뇨, 분변, 뇌척수액, 정자, 타액, 조직 및 세포 배양물과 같은 핵산-함유 생물학적 물질인 것을 특징으로 하는 방법.
  3. 제1항 또는 제2항에 있어서, 사용하는 카오트로픽 물질이 구아니디늄염, 요오드화나트륨, 요오드화칼륨, 소듐(이소)티오시아네이트, 우레아 및 이들 상호간의 조합물들로 구성된 군으로부터 선택되는 것을 특징으로 하는 방법.
  4. 제3항에 있어서, 사용하는 구아니디늄염이 구아니디늄(이소)티오시아네이트인 것을 특징으로 하는 방법.
  5. 제1항에 있어서, 사용하는 핵산-결합 고상이 실리카입자, 중합물질, 필터물질, 폴리스티렌 비이드 및 니트로셀룰로오스 종이로 구성된 군으로부터 선택되는 것을 특징으로 하는 방법.
  6. 제1항 내지 제5항중 어느 한 항에 있어서, DNA 및/또는 RNA를 분리하는 것을 특징으로 하는 방법.
  7. 제1항 내지 제6항중 어느 한 항에 있어서, 주로 0.05㎛내지500㎛범위내의 입자크기를 갖는 실리카 입자들을 사용하는 것을 특징으로 하는 방법.
  8. 제1항 내지 제6항중 어느 한 항에 있어서, 주로 0.1내지200㎛범위내의 입자크기를 갖는 실리카 입자들을 사용하는 것을 특징으로 하는 방법.
  9. 제1항 내지 제8항중 어느 한 항에 있어서, 주로 1내지200㎛범위내의 입자크기를 갖는 실리카 입자들을 사용하는 것을 특징으로 하는 방법.
  10. 제1항 내지 제9항중 어느 한 항에 있어서, 생성된 고상-핵산 복합체를 침강 및 상층액 제거처리하여 액으로부터 분리한후, 복합체를 카오트로픽 물질-함유 세정 완층액으로 세정하는 것을 특징으로 하는 방법.
  11. 제10항에 있어서, 세정 완충액으로 세정한 고상-핵산 복합체를 1종 또는 그 이상의 유기용매로 연속적으로 다시 세정한후, 건조시키는 것을 특징으로 하는 방법.
  12. 제11항에 있어서, 세정 및 건조시킨 고상-핵산 복합체내에 존재하는 핵산을 용출 완충액을 사용하여 용리시키는 것을 특징으로 하는 방법.
  13. 제1항에 있어서, 수득된 고상-핵산 복합체를 상기 고상에 결합되거나 또는 그로부터 용리된 핵산을 확장시키기 위한 목적으로 존재하는 성분들의 혼합물과 접촉시키는 것을 특징으로 하는 방법.
  14. 제1항에 기재한 방법을 수행하기 위한 장치들의 조합체.
  15. 제13항에 기재한 방법을 수행하기 위한 시험키트.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019900004012A 1989-03-23 1990-03-22 핵산 분리방법 KR0148693B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL8900725A NL8900725A (nl) 1989-03-23 1989-03-23 Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
NL8900725 1989-03-23

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KR900014594A true KR900014594A (ko) 1990-10-24
KR0148693B1 KR0148693B1 (ko) 1998-08-01

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EP (2) EP0819696A3 (ko)
JP (3) JP2680462B2 (ko)
KR (1) KR0148693B1 (ko)
AT (1) ATE156830T1 (ko)
AU (1) AU641641B2 (ko)
CA (2) CA2271603A1 (ko)
DE (2) DE389063T1 (ko)
DK (1) DK0389063T4 (ko)
ES (1) ES2085245T5 (ko)
GR (2) GR960300019T1 (ko)
NL (1) NL8900725A (ko)
ZA (1) ZA902190B (ko)

Families Citing this family (181)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992018514A1 (en) * 1991-04-12 1992-10-29 Minnesota Mining And Manufacturing Company Purification of nucleic acids using metal oxide supports
US5329000A (en) * 1991-10-31 1994-07-12 Becton, Dickinson And Company Purification of DNA with silicon tetrahydrazide
DE4143639C2 (de) * 1991-12-02 2002-10-24 Qiagen Gmbh Verfahren zur Isolierung und Reinigung von Nukleinsäuren
DE4321904B4 (de) * 1993-07-01 2013-05-16 Qiagen Gmbh Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen
GB9314249D0 (en) * 1993-07-09 1993-08-18 Proofname Ltd Purification method and apparatus
AU7375794A (en) * 1993-07-28 1995-02-28 Akzo Nobel N.V. Process for isolating nucleic acid from gram positive microorganisms
JP3451667B2 (ja) * 1993-08-24 2003-09-29 東ソー株式会社 核酸抽出及び特定核酸配列の検出方法
AU689815B2 (en) * 1993-08-30 1998-04-09 Promega Corporation Nucleic acid purification compositions and methods
US6043032A (en) * 1993-09-22 2000-03-28 Tosoh Corporation Method of extracting nucleic acids and method of detecting specified nucleic acid sequences
GB2282138B (en) * 1993-09-28 1997-09-03 Tosoh Corp Method of extracting nucleic acids and method of detecting specified nucleic acid sequences
GB9323305D0 (en) * 1993-11-11 1994-01-05 Medinnova Sf Isoaltion of nucleic acid
ATE302208T1 (de) 1994-02-11 2005-09-15 Qiagen Gmbh Verfahren zur trennung von doppelstrang/einzelstrangnukleinsäurestrukturen
WO1995034569A1 (de) * 1994-06-14 1995-12-21 Invitek Gmbh Universelles verfahren zur isolierung und reinigung von nukleinsäuren aus extrem geringen mengen sowie sehr stark verunreinigten unterschiedlichsten ausgangsmaterialien
DE4420900A1 (de) 1994-06-15 1995-12-21 Boehringer Mannheim Gmbh Verfahren zur mehrmaligen Entnahme von Flüssigkeiten
DE4420732A1 (de) 1994-06-15 1995-12-21 Boehringer Mannheim Gmbh Vorrichtung zur Behandlung von Nukleinsäuren aus einer Probe
US6110428A (en) * 1994-08-29 2000-08-29 Akzo Nobel N.V. Device for use in the isolation of a biological material such as nucleic acid
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
NL9402122A (nl) * 1994-12-14 1996-07-01 Univ Nijmegen Werkwijze voor het bereiden van aan kunststof gekoppelde niet-eiwitachtige macrobiomoleculen welke sterk negatief geladen zijn, aan kunststof gekoppelde negatief geladen niet-eiwitachtige macrobiomoleculen alsmede een microtiterplaat waarvan de putjes zijn bekleed met negatief geladen niet-eiwitachtige macrobiomoleculen.
US5576196A (en) * 1995-01-13 1996-11-19 Vical Incorporated Process for reducing RNA concentration in a mixture of biological material using diatomaceous earth
US6919175B1 (en) 1995-04-01 2005-07-19 Roche Diagnostics Gmbh System for releasing and isolating nucleic acids
DE19512368A1 (de) * 1995-04-01 1996-10-02 Boehringer Mannheim Gmbh System zur Freisetzung und Isolierung von Nukleinsäuren
KR100463475B1 (ko) 1995-06-08 2005-06-22 로셰 디아그노스틱스 게엠베하 자기성피그먼트
DE19520398B4 (de) * 1995-06-08 2009-04-16 Roche Diagnostics Gmbh Magnetisches Pigment
JP2965131B2 (ja) 1995-07-07 1999-10-18 東洋紡績株式会社 核酸結合用磁性担体およびそれを用いる核酸単離方法
EP1260595B1 (en) * 1995-07-07 2006-09-13 Toyo Boseki Kabushiki Kaisha Nucleic acid-bondable magnetic carrier and method for isolating nucleic acid using the same
DE19530132C2 (de) * 1995-08-16 1998-07-16 Max Planck Gesellschaft Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien
US5783686A (en) * 1995-09-15 1998-07-21 Beckman Instruments, Inc. Method for purifying nucleic acids from heterogenous mixtures
WO1997030062A1 (en) * 1996-02-14 1997-08-21 Akzo Nobel N.V. Isolation and amplification of nucleic acid materials
DE19607202A1 (de) * 1996-02-26 1997-08-28 Uwe Dr Michel Reagenzienkit zur Präparation von Nukleinsäuren
NL1002781C1 (nl) * 1996-04-03 1997-10-06 Amsterdam Support Diagnostics Isolatie en/of amplificatie van hepatitis-C-virus-(HCV) -nucleïnezuren uit monsters waarvan vermoed wordt dat zij HCV bevatten.
EP0814156B1 (en) * 1996-06-18 2003-03-05 The Institute Of Physical & Chemical Research Method for the purification of DNA
EP0818461B1 (en) * 1996-07-12 2005-09-28 Toyo Boseki Kabushiki Kaisha Method for isolating ribonucleic acid
US6210881B1 (en) 1996-12-30 2001-04-03 Becton, Dickinson And Company Method for reducing inhibitors of nucleic acid hybridization
US5763185A (en) * 1996-12-30 1998-06-09 Becton Dickinson And Company Method for reducing inhibitors of nucleic acid hybridization
GB9709728D0 (en) 1997-05-13 1997-07-02 Dynal As Single step method
ATE248631T1 (de) * 1997-06-27 2003-09-15 Life Technologies Inc Einstufige vorrichtung und verfahren zur konzentration und säuberung biologischer moleküle
US6881537B1 (en) 1997-08-08 2005-04-19 Biomerieux, B.V. Nucleic acid sequences that can be used as primers and probes in the amplification and detection of all subtypes of HIV-1
JP2001516731A (ja) 1997-09-17 2001-10-02 ジエントラ・システムズ・インコーポレーテツド 核酸を単離するための装置および方法
JP4304348B2 (ja) * 1997-09-22 2009-07-29 独立行政法人理化学研究所 Ｄｎａの単離方法
DE19746874A1 (de) * 1997-10-23 1999-04-29 Qiagen Gmbh Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen
JP4025399B2 (ja) * 1997-10-28 2007-12-19 株式会社日立製作所 核酸の回収方法及び装置
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
WO1999032616A2 (de) * 1997-12-18 1999-07-01 Invitek Gmbh Verfahren zur isolierung von kurz- und langkettigen nukleinsäuren
ZA99493B (en) * 1998-01-30 1999-07-22 Akzo Nobel Nv Method for the isolation of nucleic acid.
EP1053311B1 (en) * 1998-02-02 2007-11-28 QIAGEN North American Holdings, Inc. Compositions and methods for using a lysing matrix for isolating dna
DE69938774D1 (de) 1998-02-02 2008-07-03 Qiagen North American Holdings Verfahren zur isolierung, amplifizierung und charakterisierung von dns
WO1999039009A1 (en) * 1998-02-02 1999-08-05 Gentra Systems, Inc. Processes for isolating, amplifying and characterizing dna
AU2574299A (en) * 1998-02-02 1999-08-16 Gentra Systems, Inc. Eluting reagents, methods and kits for isolating dna
JPH11266864A (ja) * 1998-03-19 1999-10-05 Hitachi Ltd 核酸の精製方法および精製用装置
DE19818485B4 (de) * 1998-04-24 2005-03-24 IBL Gesellschaft für Immunchemie und Immunbiologie mbH Reaktionsgefäße, beschichtet mit einem cis-Diol-spezifischen Affinitätsmedium, sowie Verfahren zu deren Herstellung
DE19836559A1 (de) 1998-08-12 2000-03-23 Antigen Gmbh Gefäß zur Entnahme von Blut
EP1109899B1 (en) * 1998-09-10 2007-02-21 WHATMAN plc Product and method for separation of a sample containing multiple sources of genetic material using a solid medium
US6958392B2 (en) * 1998-10-09 2005-10-25 Whatman, Inc. Methods for the isolation of nucleic acids and for quantitative DNA extraction and detection for leukocyte evaluation in blood products
JP2000166556A (ja) * 1998-12-10 2000-06-20 Hitachi Ltd 核酸の回収方法及び装置
US6492162B1 (en) 1998-10-27 2002-12-10 Hitachi, Ltd. Apparatus for the recovery of nucleic acids
US7790865B1 (en) 1999-02-02 2010-09-07 Qiagen North American Holdings, Inc Eluting reagents, methods and kits for isolating DNA
CA2270106C (en) * 1999-04-23 2006-03-14 Yousef Haj-Ahmad Nucleic acid purification and process
WO2001010554A2 (de) * 1999-08-09 2001-02-15 Bilatec Ag Labor-roboter und verfahren und reagenzienkit zur isolierung von nukleinsäuren
DE19943374A1 (de) 1999-09-10 2001-03-29 Max Planck Gesellschaft Verfahren zum Anbinden von Nukleinsäuren an eine Festphase
US6383783B1 (en) 1999-09-21 2002-05-07 3M Innovative Properties Company Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant
DE19946597A1 (de) * 1999-09-29 2001-04-12 Nigu Chemie Gmbh Stabilisiertes Guanidinlthiocyanat und dessen Verwendung
CA2392009C (en) 1999-11-17 2009-07-14 Roche Diagnostics Gmbh Magnetic glass particles, method for their preparation and uses thereof
GB0001450D0 (en) 2000-01-21 2000-03-08 Genpoint As Cell isolation method
DE10004927C2 (de) * 2000-02-04 2003-02-06 Qiagen Gmbh Nukleinsäure-Isolierung aus Stuhlproben und anderen biologischen Materialien, die reich an Inhibitoren sind
DE10006662A1 (de) * 2000-02-15 2001-08-23 Antigen Produktions Gmbh Gefäß zur Nukleinsäureanalytik
US6815215B2 (en) 2000-02-28 2004-11-09 Hitachi, Ltd. Method of recovering a plurality of nucleic acids by an identical stationary phase and an apparatus thereof
WO2001071732A2 (en) 2000-03-24 2001-09-27 Qiagen Gmbh Porous ferro- or ferrimagnetic glass particles for isolating molecules
GB0009771D0 (en) 2000-04-19 2000-06-07 Angiomed Ag Method for linking nucleic acids
GB0013658D0 (en) 2000-06-05 2000-07-26 Dynal Asa Nucleic acid isolation
WO2002078847A1 (fr) 2001-03-28 2002-10-10 Hitachi, Ltd. Instrument et procede permettant de recuperer de l'acide nucleique
US6818762B2 (en) 2001-05-25 2004-11-16 Maine Molecular Quality Controls, Inc. Compositions and methods relating to nucleic acid reference standards
JP2002191351A (ja) * 2001-10-19 2002-07-09 Hitachi Ltd 核酸の精製用装置および核酸捕捉用チップ
AU2002359522A1 (en) 2001-11-28 2003-06-10 Applera Corporation Compositions and methods of selective nucleic acid isolation
EP2913407B1 (en) 2002-01-07 2017-11-01 Pretect AS Method for detecting human papillomavirus mrna
AU2003235767A1 (en) 2002-01-08 2003-07-24 Roche Diagnostics Gmbh Use of silica material in an amplification reaction
WO2003062462A2 (en) 2002-01-16 2003-07-31 Dynal Biotech Asa Method for isolating nucleic acids and protein from a single sample
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
DE10231659B4 (de) * 2002-07-12 2006-01-19 Preanalytix Gmbh Zusammensetzung zum Binden von Nukleinsäure an eine Festphase
US9394332B2 (en) 2002-08-29 2016-07-19 Epigenomics Ag Method for bisulfite treatment
WO2004079333A2 (en) * 2003-01-15 2004-09-16 Ic & G Co., Ltd. Method and kit for extracting rna from sputum
WO2004067545A1 (en) 2003-01-29 2004-08-12 Roche Diagnostics Gmbh Improved method for bisulfite treatment
US7601491B2 (en) 2003-02-06 2009-10-13 Becton, Dickinson And Company Pretreatment method for extraction of nucleic acid from biological samples and kits therefor
ATE451617T1 (de) 2003-03-19 2009-12-15 Hamamatsu Found Sci & Tech Pro Verfahren zur erkennung eines kolonkrebsmarkers
EP1641944B1 (en) * 2003-07-09 2011-06-29 Genvault Corporation Room temperature elution of nucleic acids
US20060186055A1 (en) * 2003-07-16 2006-08-24 Toyo Boseki Kabushiki Kaisha Device for separation of biological components, and method of separation of biological components using the device
JP4871722B2 (ja) † 2003-07-25 2012-02-08 アプライド バイオシステムズ リミテッド ライアビリティー カンパニー 固定試料からｒｎａを調製するための方法および組成物
US20050059024A1 (en) 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
GB0319671D0 (en) * 2003-08-21 2003-09-24 Secr Defence Apparatus for processing a fluid sample
EP1692315A1 (en) 2003-12-02 2006-08-23 Roche Diagnostics GmbH Improved method for bisulfite treatment
US20050130177A1 (en) 2003-12-12 2005-06-16 3M Innovative Properties Company Variable valve apparatus and methods
DE10358137A1 (de) * 2003-12-12 2005-07-07 Merck Patent Gmbh Verfahren und Kit zur Isolierung von RNA
WO2005064010A1 (de) * 2003-12-19 2005-07-14 Preanalytix Gmbh Zusammensetzung zum binden von nukleinsäure an eine festphase
US7939249B2 (en) 2003-12-24 2011-05-10 3M Innovative Properties Company Methods for nucleic acid isolation and kits using a microfluidic device and concentration step
US7727710B2 (en) 2003-12-24 2010-06-01 3M Innovative Properties Company Materials, methods, and kits for reducing nonspecific binding of molecules to a surface
EP1719816B1 (en) 2004-02-12 2011-06-01 GL Sciences Incorporated Mechanism of separating and purifying dna
AU2005200670B2 (en) 2004-02-20 2007-05-03 F. Hoffmann-La Roche Ag Adsorption of nucleic acids to a solid phase
JP4539125B2 (ja) * 2004-03-08 2010-09-08 ブラザー工業株式会社 核酸単離部材
US20050208501A1 (en) * 2004-03-16 2005-09-22 Ambion, Inc. Process and reagents for extraction of RNA from fractionated blood leukocytes
US7897378B2 (en) 2004-03-18 2011-03-01 Roche Molecular Systems, Inc. Method and device for purifying nucleic acids
JP4080462B2 (ja) * 2004-07-09 2008-04-23 株式会社日立製作所 核酸の回収方法
DE102004034433A1 (de) * 2004-07-15 2006-02-02 Qiagen Gmbh Verfahren zur Reinigung und Isolierung von Nukleinsäuren unter Verwendung kationischer Detergentien
EP1632578A1 (en) 2004-09-03 2006-03-08 Roche Diagnostics GmbH DNA decontamination method
JP2006166711A (ja) * 2004-12-13 2006-06-29 Okayama Univ 糞便由来遺伝子の簡便増幅方法
JP4764966B2 (ja) * 2005-03-30 2011-09-07 独立行政法人科学技術振興機構 メッセンジャーｒｎａの分離方法
DE102005040259A1 (de) 2005-08-24 2007-03-01 Qiagen Gmbh Verfahren zur Gewinnung von Nukleinsäuren aus Blut
JP4699868B2 (ja) * 2005-11-04 2011-06-15 株式会社日立ハイテクノロジーズ 核酸精製方法及び核酸精製器具
US7981606B2 (en) 2005-12-21 2011-07-19 Roche Molecular Systems, Inc. Control for nucleic acid testing
US20100178510A1 (en) 2006-06-20 2010-07-15 Hitachi Metals, Ltd. Fine metal particles and biomaterial-extracting magnetic beads, and their production methods
JP2008005781A (ja) * 2006-06-29 2008-01-17 Olympus Corp 試料処理方法
US20080113357A1 (en) * 2006-06-29 2008-05-15 Millipore Corporation Filter device for the isolation of a nucleic acid
US10131935B2 (en) * 2006-07-11 2018-11-20 Aj Innuscreen Gmbh Method for parallel isolation of viral nucleic acids
EP1911844A1 (en) 2006-10-10 2008-04-16 Qiagen GmbH Methods and kit for isolating nucleic acids
EP1932913B1 (en) 2006-12-11 2013-01-16 Roche Diagnostics GmbH Nucleic acid isolation using polidocanol and derivatives
JP5354894B2 (ja) 2006-12-11 2013-11-27 エフ．ホフマン−ラ ロシュ アーゲー ポリドカノールおよび誘導体を用いた核酸単離
WO2008079905A1 (en) * 2006-12-21 2008-07-03 Invitrogen Dynal As Particles and their use in a method for isolating nucleic acid or a method for isolating phosphoproteins
DE102007009347B4 (de) 2007-02-27 2009-11-26 Agowa Gmbh Verfahren zur Isolierung von Nukleinsäuren
DE102007016707A1 (de) 2007-04-04 2008-10-09 Qiagen Gmbh Verfahren zur Aufreinigung von Biomolekülen
CA2629589C (en) * 2007-04-20 2016-03-29 F.Hoffmann-La Roche Ag Isolation and purification of nucleic acid molecules with a solid phase
CA2629586C (en) * 2007-04-20 2016-05-24 F.Hoffmann-La Roche Ag Adsorption of nucleic acids to solid phases under low-salt conditions
DE102007035250A1 (de) * 2007-07-27 2009-01-29 Qiagen Gmbh Verfahren zum Abtrennen von nicht-proteinhaltigen Biomolekülen, insbesondere Nukleinsäuren aus proteinhaltigen Proben
EP2036988A1 (en) 2007-09-12 2009-03-18 Siemens Healthcare Diagnostics GmbH A method for predicting the response of a tumor in a patient suffering from or at risk of developing recurrent gynecologic cancer towards a chemotherapeutic agent
JP4097687B2 (ja) * 2007-09-21 2008-06-11 株式会社日立製作所 核酸の回収方法
EP2065399A1 (en) 2007-11-30 2009-06-03 Siemens Healthcare Diagnostics GmbH The present invention relates to methods for prediction of the therapeutic success of breast cancer therapy
EP2055385B1 (en) 2007-10-31 2010-05-26 Roche Diagnostics GmbH Method and device for purifying nucleic acids
EP2065474A1 (en) 2007-11-28 2009-06-03 Siemens Healthcare Diagnostics GmbH A method to assess prognosis and to predict therapeutic response to endocrine treatment
WO2009068423A2 (en) 2007-11-30 2009-06-04 Siemens Healthcare Diagnostics Gmbh Method for predicting therapy responsiveness in basal like tumors
EP2065475A1 (en) 2007-11-30 2009-06-03 Siemens Healthcare Diagnostics GmbH Method for therapy prediction in tumors having irregularities in the expression of at least one VEGF ligand and/or at least one ErbB-receptor
EP2071034A1 (en) 2007-12-12 2009-06-17 bioMérieux Method for treating a solution in order to destroy any ribonucleic acid after amplification
DE102008010692A1 (de) 2008-02-22 2009-08-27 Qiagen Gmbh Neue Matrices, Verfahren zu ihrer Herstellung und ihre Verwendung in Verfahren zur Isolierung von Biomolekülen
DE102008010693A1 (de) 2008-02-22 2009-08-27 Qiagen Gmbh Neue Matrixmaterialien, Verfahren zu ihrer Herstellung und ihre Verwendung in Verfahren zur Isolierung von Biomolekülen
EP2128169A1 (de) 2008-05-30 2009-12-02 Qiagen GmbH Verfahren zur Isolierung von kurzkettigen Nukleinsäuren
DE102008029356A1 (de) * 2008-06-20 2009-12-24 Siemens Healthcare Diagnostics Gmbh Verfahren zur Aufreinigung von Nukleinsäuren, insbesondere aus fixiertem Gewebe
US8546127B2 (en) 2008-06-30 2013-10-01 General Electric Company Bacteria/RNA extraction device
US8158405B2 (en) 2008-06-30 2012-04-17 General Electric Company Process for concentrating and processing fluid samples
ES2539856T3 (es) 2008-08-01 2015-07-06 F. Hoffmann-La Roche Ag Lisis mejorada y transcripción reversa para la cuantificación de mRNA
EP2157181A1 (de) 2008-08-13 2010-02-24 AGOWA Gesellschaft für molekularbiologische Technologie mbH Verfahren zur Isolation von Nukleinsäuren und Testkit
DE102008047790A1 (de) 2008-09-17 2010-04-15 Qiagen Gmbh Verfahren zur Normierung des Gehalts von Biomolekülen in einer Probe
CA2682439A1 (en) 2008-10-17 2010-04-17 F. Hoffmann-La Roche Ag Cell monitoring and molecular analysis
DE102008061714A1 (de) 2008-12-12 2010-06-17 Siemens Healthcare Diagnostics Inc., Deerfield Verfahren zur Aufreinigung von Nukleinsäuren, inbesondere aus fixiertem Gewebe
FR2940805B1 (fr) 2009-01-05 2015-10-16 Biomerieux Sa Procede d'amplification et/ou de detection d'acides nucleiques, kits et utilisations de ce procede
JP5570422B2 (ja) 2009-01-16 2014-08-13 アークレイ株式会社 核酸試料の製造方法、および、それを用いた核酸増幅物の製造方法
DE102009022513A1 (de) 2009-05-25 2010-12-02 Qiagen Gmbh Verfahren zum Schutz von Membranen
BR112012002401A2 (pt) * 2009-08-04 2019-09-24 Du Pont processo para coletar ácidos nucleicos de micro-organismos a partir de uma amostra particulada
FR2959312B1 (fr) 2010-04-23 2023-01-20 Biomerieux Sa Dispositif de preparation et/ou de traitement d'un echantillon biologique
JP5454338B2 (ja) 2010-04-28 2014-03-26 株式会社島津製作所 液滴操作によるリアルタイム核酸増幅法
CN102276937B (zh) * 2010-06-12 2013-06-05 中国石油化工股份有限公司 一种抗菌聚苯乙烯组合物及其制备方法
GB201010237D0 (en) 2010-06-18 2010-07-21 Lgc Ltd Methods and apparatuses
US9725754B2 (en) 2010-07-29 2017-08-08 Sean F. Boyle Generic sample preparation
DE112011102524T5 (de) 2010-07-29 2013-07-11 Roche Diagnostics Gmbh Präparation generischer Proben
DE102010043015B4 (de) * 2010-10-27 2019-12-19 Robert Bosch Gmbh Verfahren zur Konzentration von Probenbestandteilen und Amplifikation von Nukleinsäuren
US10767214B2 (en) 2010-10-28 2020-09-08 Clinical Genomics Pty Ltd Method of microvesicle enrichment
JP5455878B2 (ja) 2010-11-30 2014-03-26 シスメックス株式会社 被検物質の電気化学的検出方法
US9517472B2 (en) 2010-12-21 2016-12-13 Shimadzu Corporation Device and method for processing target component in tube
KR101193765B1 (ko) * 2011-05-16 2012-10-24 (주)나노헬릭스 초고속 핵산의 정제방법
EP2546199A1 (en) 2011-07-14 2013-01-16 Université Catholique De Louvain Solid iron oxide support for isolation of microorganisms
JP5807542B2 (ja) 2011-12-22 2015-11-10 株式会社島津製作所 対象成分を操作するためのチップデバイス及びそれを用いた方法
CN104350152B (zh) * 2012-06-01 2017-08-11 欧米伽生物技术公司 选择性核酸片段回收
DE102012012523B4 (de) 2012-06-26 2015-02-12 Magnamedics Gmbh Reinigung von Nukleinsäuren
JP6024266B2 (ja) * 2012-08-01 2016-11-16 セイコーエプソン株式会社 Ｄｎａの抽出方法
JP6093530B2 (ja) * 2012-09-24 2017-03-08 東芝メディカルシステムズ株式会社 乳中の微生物を濃縮および乳中の微生物の核酸を回収する方法
JP6255034B2 (ja) 2012-12-11 2017-12-27 キアゲン ゲーエムベーハー シリカ粒子の調製
WO2014122288A1 (en) 2013-02-08 2014-08-14 Qiagen Gmbh Method for separating dna by size
AU2013202805B2 (en) 2013-03-14 2015-07-16 Gen-Probe Incorporated System and method for extending the capabilities of a diagnostic analyzer
EP2969140B1 (en) 2013-03-15 2021-02-17 Abbott Molecular Inc. One-step procedure for the purification of nucleic acids
CN105358688B (zh) 2013-07-03 2019-01-18 株式会社岛津制作所 粒子操作方法以及粒子操作器件
JP6629185B2 (ja) * 2014-03-26 2020-01-15 ユニバーサル・バイオ・リサーチ株式会社 核酸抽出方法及び試薬
CN117402871A (zh) * 2014-04-25 2024-01-16 川斯勒佰尔公司 信使rna的纯化方法
WO2016016345A1 (en) 2014-07-30 2016-02-04 F. Hoffmann - La Roche Ag Automated system for processing particles
JP6437250B2 (ja) 2014-09-03 2018-12-12 株式会社ミズホメディー 前処理方法及びそれに用いられる核酸抽出キット
US20180291365A1 (en) 2015-06-05 2018-10-11 Qiagen Gmbh Method for separating dna by size
EP3307907B1 (en) 2015-06-09 2023-11-15 Biocartis NV Automatable method for nucleic acid isolation
JPWO2017043649A1 (ja) 2015-09-10 2018-07-19 株式会社カネカ 核酸を含有する検体から核酸を分離する方法及びそのための装置
BR112018013153A2 (pt) 2015-12-28 2018-12-11 Koninklijke Philips N.V. método para a purificação de ácidos nucleicos, kit para purificar ácidos nucleicos e cartucho para executar o método
TWI694146B (zh) * 2016-02-02 2020-05-21 日商美之賀美蒂股份有限公司 前處理方法及用於其之核酸萃取套組
EP3205722B1 (de) * 2016-02-11 2018-08-01 Sarstedt AG & Co. KG Vorrichtung und verfahren zur isolierung von nukleinsäuren aus vollblut
MD3445850T2 (ro) * 2016-04-22 2022-01-31 BioNTech SE Metode de obținere a ARN monocatenar
JP6979797B2 (ja) * 2017-05-31 2021-12-15 シスメックス株式会社 固液分離方法、固液分離装置およびそれに用いるピペットチップ、粒子およびキット
CN108130325A (zh) * 2018-02-02 2018-06-08 苏州优卡新材料科技有限公司 一种核糖核酸提取用无机材料滤芯的高效纯化柱
JP2020024202A (ja) * 2018-08-06 2020-02-13 積水化学工業株式会社 分析用具及び洗浄方法
CN113544281A (zh) 2018-11-19 2021-10-22 拜奥卡蒂斯生物股份有限公司 集成工作流中低拷贝数核酸的增强的检测
EP4010488A1 (en) 2019-08-08 2022-06-15 Biocartis NV Novel nucleic acid purification chemistry

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59227744A (ja) * 1983-06-08 1984-12-21 Rikaken Kk Ｄｎａ回収用ガラス粉粒物
US4900677A (en) * 1986-09-26 1990-02-13 E. I. Du Pont De Nemours And Company Process for rapid isolation of high molecular weight DNA

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