ES2085245T3 - Procedimiento de purificacion de acidos nucleicos. - Google Patents

Procedimiento de purificacion de acidos nucleicos.

Info

Publication number
ES2085245T3
ES2085245T3 ES90200678T ES90200678T ES2085245T3 ES 2085245 T3 ES2085245 T3 ES 2085245T3 ES 90200678 T ES90200678 T ES 90200678T ES 90200678 T ES90200678 T ES 90200678T ES 2085245 T3 ES2085245 T3 ES 2085245T3
Authority
ES
Spain
Prior art keywords
nucleic acid
purification procedure
acid purification
relates
isolating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90200678T
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English (en)
Other versions
ES2085245T5 (es
ES2085245T1 (es
Inventor
Willem Rene Boom
Henriette Maria Alei Adriaanse
Tim Kievits
Peter Franklin Lens
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Akzo Nobel NV
Original Assignee
Akzo Nobel NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=19854348&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2085245(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Akzo Nobel NV filed Critical Akzo Nobel NV
Publication of ES2085245T1 publication Critical patent/ES2085245T1/es
Publication of ES2085245T3 publication Critical patent/ES2085245T3/es
Application granted granted Critical
Publication of ES2085245T5 publication Critical patent/ES2085245T5/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Hard Magnetic Materials (AREA)

Abstract

Un proceso para aislar ácido nucleico a partir de un material de partida biológico complejo que contenga ácido nucleico, caracterizado por la mezcla del material de partida biológico complejo, una sustancia caotrópica y una fase sólida que se une al ácido nucleico conteniendo sílice o un derivado de la misma, separando la fase sólida con el ácido nucleico unido a ella del líquido, después de lo cual los complejos fase sólida-ácido nucleico así obtenidos son lavados y, si se requiere, el ácido nucleico es eluido de dichos complejos, donde se selecciona el material de partida biológica de: sangre completa, suero sanguíneo, capa amarillenta, orina, heces, líquido cerebroespinal, esperma, saliva, tejidos y cultivos celulares, productos alimenticios, vacunas, leche infectada con un virus o una bacteria, material vegetal, bacterias gram positivas, levaduras, mohos, fluidos corporales y material biológico posiblemente infectado con virus o bacterias.
ES90200678T 1989-03-23 1990-03-22 Procedimiento de purificacion de acidos nucleicos. Expired - Lifetime ES2085245T5 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL8900725A NL8900725A (nl) 1989-03-23 1989-03-23 Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
NL8900725 1989-03-23

Publications (3)

Publication Number Publication Date
ES2085245T1 ES2085245T1 (es) 1996-06-01
ES2085245T3 true ES2085245T3 (es) 1997-11-01
ES2085245T5 ES2085245T5 (es) 2007-06-01

Family

ID=19854348

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90200678T Expired - Lifetime ES2085245T5 (es) 1989-03-23 1990-03-22 Procedimiento de purificacion de acidos nucleicos.

Country Status (12)

Country Link
EP (2) EP0819696A3 (es)
JP (3) JP2680462B2 (es)
KR (1) KR0148693B1 (es)
AT (1) ATE156830T1 (es)
AU (1) AU641641B2 (es)
CA (2) CA2271603A1 (es)
DE (2) DE69031237T3 (es)
DK (1) DK0389063T4 (es)
ES (1) ES2085245T5 (es)
GR (2) GR960300019T1 (es)
NL (1) NL8900725A (es)
ZA (1) ZA902190B (es)

Families Citing this family (180)

* Cited by examiner, † Cited by third party
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DK0389063T3 (da) 1998-03-30
ZA902190B (en) 1991-12-24
ES2085245T5 (es) 2007-06-01
KR900014594A (ko) 1990-10-24
GR3025351T3 (en) 1998-02-27
AU641641B2 (en) 1993-09-30
DK0389063T4 (da) 2007-02-19
KR0148693B1 (ko) 1998-08-01
EP0389063B1 (en) 1997-08-13
NL8900725A (nl) 1990-10-16
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JP2680462B2 (ja) 1997-11-19
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CA2012777A1 (en) 1990-09-23
EP0819696A2 (en) 1998-01-21
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JPH02289596A (ja) 1990-11-29
EP0819696A3 (en) 1998-07-15
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DE69031237T3 (de) 2007-06-06
ES2085245T1 (es) 1996-06-01

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