ATE554166T1 - Verfahren und zusammensetzungen zur herstellung von rna aus einer fixierten probe - Google Patents
Verfahren und zusammensetzungen zur herstellung von rna aus einer fixierten probeInfo
- Publication number
- ATE554166T1 ATE554166T1 AT04820001T AT04820001T ATE554166T1 AT E554166 T1 ATE554166 T1 AT E554166T1 AT 04820001 T AT04820001 T AT 04820001T AT 04820001 T AT04820001 T AT 04820001T AT E554166 T1 ATE554166 T1 AT E554166T1
- Authority
- AT
- Austria
- Prior art keywords
- compositions
- methods
- rna
- present
- fixed sample
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Sampling And Sample Adjustment (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US49032503P | 2003-07-25 | 2003-07-25 | |
| PCT/US2004/024155 WO2005054466A2 (en) | 2003-07-25 | 2004-07-26 | Methods and compositions for preparing rna from a fixed sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE554166T1 true ATE554166T1 (de) | 2012-05-15 |
Family
ID=34652241
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04820001T ATE554166T1 (de) | 2003-07-25 | 2004-07-26 | Verfahren und zusammensetzungen zur herstellung von rna aus einer fixierten probe |
Country Status (6)
| Country | Link |
|---|---|
| US (5) | US20050059054A1 (de) |
| EP (2) | EP2295569A1 (de) |
| JP (1) | JP4871722B2 (de) |
| CN (2) | CN1878866B (de) |
| AT (1) | ATE554166T1 (de) |
| WO (1) | WO2005054466A2 (de) |
Families Citing this family (45)
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| JP4579234B2 (ja) * | 2003-03-10 | 2010-11-10 | エクスプレッション、パソロジー、インコーポレイテッド | 組織病理学的に加工処理された生物サンプル、組織および細胞からの液体組織調製物 |
| US20050059024A1 (en) * | 2003-07-25 | 2005-03-17 | Ambion, Inc. | Methods and compositions for isolating small RNA molecules |
| AU2005305012C1 (en) * | 2004-11-05 | 2012-07-19 | Qiagen North American Holdings, Inc. | Compositions and methods for purifying nucleic acids from stabilization reagents |
| US7541144B2 (en) | 2005-01-31 | 2009-06-02 | Agilent Technologies, Inc. | RNA labeling method |
| US8076064B2 (en) | 2005-07-09 | 2011-12-13 | Agilent Technologies, Inc. | Method of treatment of RNA sample |
| US7718365B2 (en) | 2005-07-09 | 2010-05-18 | Agilent Technologies, Inc. | Microarray analysis of RNA |
| US8758990B2 (en) * | 2005-07-28 | 2014-06-24 | Id-Fish Technology, Inc. | Methods for detecting and differentiating Mycobacterium genus and Mycobacterium avium complex in a sample or culture |
| PT1924252E (pt) * | 2005-07-28 | 2011-12-16 | Id Fish Technology Inc | Método para melhorar a permeabilidade celular a partículas estranhas |
| US7544471B2 (en) | 2005-07-30 | 2009-06-09 | Agilent Technologies, Inc. | Preparing RNA from a wax-embedded tissue specimen |
| US7700289B2 (en) | 2006-04-03 | 2010-04-20 | Agilent Technologies, Inc. | Method for labeling RNA |
| DE102006031764B4 (de) * | 2006-07-06 | 2009-10-01 | Aj Innuscreen Gmbh | Verfahren zur parallelen Isolierung doppel- und einzelsträngiger Nukleinsäuren sowie zur selektiven Entfernung doppelsträngiger Nukleinsäuren aus einem Gemisch von doppel- und einzelsträngigen Nukleinsäuren |
| JPWO2008038813A1 (ja) * | 2006-09-28 | 2010-01-28 | 株式会社島津製作所 | パラフィン包埋標本の脱パラフィン法及びパラフィン包埋標本の解析法 |
| EP2173874A2 (de) | 2007-06-22 | 2010-04-14 | Response Genetics, Inc | Verfahren zur isolierung von rna in langen fragmenten aus fixierten proben |
| MY157651A (en) * | 2007-07-23 | 2016-07-15 | Yiap Beow Chin | A method, kit and apparatus for extracting biological materials |
| US20090035748A1 (en) * | 2007-08-01 | 2009-02-05 | Lizzi Tiffiny Marie | Bromelain as a clinical sample pre-treatment, lysis agent and nuclease inhibitor |
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| EP2193831A1 (de) | 2008-12-05 | 2010-06-09 | Qiagen GmbH | Parallel-Extraktion von unterschiedlichen Biomolekülen aus Formalin-fixiertem Gewebe |
| EP2208785A1 (de) | 2009-01-15 | 2010-07-21 | Newbiotechnic, S.A. | Verfahren und Kits zum Erzeugen von miRNA- und smallRNA-Expressionsvektoren und ihre Anwendung zur Entwicklung von lentiviralen Expressionsbibliotheken |
| DE102009025574A1 (de) * | 2009-06-19 | 2010-12-23 | Leica Biosystems Nussloch Gmbh | Verfahren zum automatischen Bearbeiten von Gewebeproben in einem Gewebeprozessor |
| WO2011008553A1 (en) * | 2009-06-29 | 2011-01-20 | Life Technologies Corporation | Rna sample stabilization in the presence of a transitional metal |
| DK2539449T3 (en) * | 2010-02-26 | 2018-08-06 | Qiagen Gmbh | PROCEDURE FOR PARALLEL ISOLATION AND CLEANING RNA AND DNA |
| CN101914523B (zh) * | 2010-07-08 | 2013-04-24 | 浙江理工大学 | 从人血清/血浆样本中分离rna方法及其pcr验证法 |
| CN101935648B (zh) * | 2010-09-13 | 2013-04-17 | 原平皓(天津)生物技术有限公司 | 一种提取rna的方法和试剂盒 |
| EP2646551B1 (de) | 2010-11-30 | 2017-06-07 | Life Technologies Corporation | Alkylenglykole sowie polymere und copolymere daraus zur direkten isolierung von nukleinsäuren aus eingebetteten proben |
| EP2505664B1 (de) * | 2011-03-30 | 2014-12-03 | Universität Leipzig | Verfahren und Mittel zur Unterscheidung von gut- und bösartigen Tumorproben, insbesondere bei der FNAB |
| US20140243216A1 (en) | 2011-09-26 | 2014-08-28 | Qiagen Gmbh | Methods for separating nucleic acids by size |
| EP2811281B1 (de) * | 2012-01-31 | 2017-10-25 | National Cancer Center | Zusammensetzung zur Aggregation biologischer Proben, Verfahren zur Herstellung von Paraffinblöcken sowie Verfahren zur Analyse von Paraffinblöcken durch Mikroskopie |
| EP2888354B1 (de) | 2012-08-21 | 2020-04-01 | Qiagen GmbH | Stabilisierung von viruspartikeln und viralen nukleinsäuren in einer biologischen probe |
| WO2014029791A1 (en) * | 2012-08-21 | 2014-02-27 | Qiagen Gmbh | Method for isolating nucleic acids from a formaldehyde releaser stabilized sample |
| AU2013310861B2 (en) | 2012-09-03 | 2019-02-14 | Qiagen Gmbh | Method for isolating RNA including small RNA with high yield |
| GB201217405D0 (en) | 2012-09-28 | 2012-11-14 | Fermentas Uab | Protein removal agent |
| US9771571B2 (en) | 2014-02-28 | 2017-09-26 | Gen-Probe Incorporated | Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde |
| US9771572B2 (en) | 2014-02-28 | 2017-09-26 | Gen-Probe Incorporated | Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde |
| JP6322292B2 (ja) | 2014-02-28 | 2018-05-09 | ジェン−プローブ・インコーポレーテッド | ホルムアルデヒドを含有する液体系細胞診用保存剤中検体から核酸を単離する方法 |
| GB2528344B (en) | 2014-02-28 | 2018-10-31 | Gen Probe Inc | Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde |
| WO2016009059A1 (en) | 2014-07-17 | 2016-01-21 | Qiagen Gmbh | Method for isolating rna with high yield |
| SG10201505563XA (en) * | 2015-07-15 | 2017-02-27 | Delta Electronics Int’L Singapore Pte Ltd | Nucleic acid extraction method |
| CN105063014A (zh) * | 2015-07-28 | 2015-11-18 | 福建师范大学 | 一种利用吸附柱提取植物MicroRNA的方法 |
| PL3568475T3 (pl) | 2017-01-16 | 2023-06-19 | Spectrum Solutions L.L.C. | Roztwór do konserwacji kwasu nukleinowego i sposoby zastosowania |
| WO2019063071A1 (en) | 2017-09-27 | 2019-04-04 | Qiagen Gmbh | METHOD FOR HIGH-PERFORMANCE RNA ISOLATION |
| CN112522792B (zh) * | 2020-01-22 | 2022-12-20 | 微岩医学科技(北京)有限公司 | 一种rna测序文库的构建方法 |
| EP4193137A4 (de) * | 2020-08-10 | 2024-05-22 | Leica Biosystems Nussloch GmbH | Xylolfreie gewebeaufbereitungsvorrichtung und verfahren zur verarbeitung von gewebe |
| KR102291738B1 (ko) * | 2021-01-15 | 2021-08-20 | (주)진이어스 | 컬럼 방식의 단일튜브 pcr용 핵산 분리를 위한 버퍼 조성물 및 이의 용도 |
| CN114426970A (zh) * | 2022-03-17 | 2022-05-03 | 黑龙江省科学院高技术研究院 | 一种适合杨树rna的提取方法 |
| CN115261368A (zh) * | 2022-08-16 | 2022-11-01 | 北京思尔成生物技术有限公司 | 一种即用型胃蛋白酶工作液及其制备方法和应用 |
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| WO2003046146A2 (en) * | 2001-11-28 | 2003-06-05 | Applera Corporation | Compositions and methods of selective nucleic acid isolation |
| ATE374837T1 (de) * | 2002-01-08 | 2007-10-15 | Hoffmann La Roche | Verwendung eines silikamaterials bei der amplifikation |
| WO2003078662A1 (en) † | 2002-03-13 | 2003-09-25 | Genomic Health | Gene expression profiling in biopsied tumor tissues |
| US7364846B2 (en) † | 2002-10-11 | 2008-04-29 | Molecular Devices Corporation | Gene expression profiling from FFPE samples |
| WO2005012487A2 (en) * | 2003-08-01 | 2005-02-10 | Invitrogen Corporation | Compositions and methods for preparing short rna molecules and other nucleic acids |
| US8312249B1 (en) | 2008-10-10 | 2012-11-13 | Apple Inc. | Dynamic trampoline and structured code generation in a signed code environment |
| FR2957821B1 (fr) | 2010-03-24 | 2014-08-29 | Inst Francais Du Petrole | Nouvelle zone de regeneration du catalyseur divisee en secteurs pour unites catalytiques regeneratives |
-
2004
- 2004-07-26 AT AT04820001T patent/ATE554166T1/de active
- 2004-07-26 EP EP10184549A patent/EP2295569A1/de not_active Ceased
- 2004-07-26 CN CN2004800251339A patent/CN1878866B/zh not_active Expired - Fee Related
- 2004-07-26 WO PCT/US2004/024155 patent/WO2005054466A2/en not_active Ceased
- 2004-07-26 US US10/899,386 patent/US20050059054A1/en not_active Abandoned
- 2004-07-26 JP JP2006521996A patent/JP4871722B2/ja not_active Expired - Lifetime
- 2004-07-26 EP EP04820001.8A patent/EP1654361B2/de not_active Expired - Lifetime
- 2004-07-26 CN CN200480025190.7A patent/CN1882688B/zh not_active Expired - Fee Related
-
2009
- 2009-08-05 US US12/536,073 patent/US20100035770A1/en not_active Abandoned
-
2014
- 2014-03-12 US US14/206,642 patent/US20140329703A1/en not_active Abandoned
-
2016
- 2016-05-23 US US15/162,373 patent/US20160333394A1/en not_active Abandoned
-
2019
- 2019-12-12 US US16/712,102 patent/US20200109440A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20160333394A1 (en) | 2016-11-17 |
| US20140329703A1 (en) | 2014-11-06 |
| CN1878866B (zh) | 2012-08-01 |
| US20200109440A1 (en) | 2020-04-09 |
| CN1878866A (zh) | 2006-12-13 |
| EP1654361B2 (de) | 2023-01-18 |
| CN1882688B (zh) | 2014-07-16 |
| EP2295569A1 (de) | 2011-03-16 |
| WO2005054466A2 (en) | 2005-06-16 |
| EP1654361A2 (de) | 2006-05-10 |
| CN1882688A (zh) | 2006-12-20 |
| EP1654361B1 (de) | 2012-04-18 |
| WO2005054466A3 (en) | 2005-12-15 |
| JP2007500009A (ja) | 2007-01-11 |
| US20100035770A1 (en) | 2010-02-11 |
| JP4871722B2 (ja) | 2012-02-08 |
| US20050059054A1 (en) | 2005-03-17 |
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