ATE554166T1 - Verfahren und zusammensetzungen zur herstellung von rna aus einer fixierten probe - Google Patents
Verfahren und zusammensetzungen zur herstellung von rna aus einer fixierten probeInfo
- Publication number
- ATE554166T1 ATE554166T1 AT04820001T AT04820001T ATE554166T1 AT E554166 T1 ATE554166 T1 AT E554166T1 AT 04820001 T AT04820001 T AT 04820001T AT 04820001 T AT04820001 T AT 04820001T AT E554166 T1 ATE554166 T1 AT E554166T1
- Authority
- AT
- Austria
- Prior art keywords
- compositions
- methods
- rna
- present
- fixed sample
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Sampling And Sample Adjustment (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US49032503P | 2003-07-25 | 2003-07-25 | |
| PCT/US2004/024155 WO2005054466A2 (en) | 2003-07-25 | 2004-07-26 | Methods and compositions for preparing rna from a fixed sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE554166T1 true ATE554166T1 (de) | 2012-05-15 |
Family
ID=34652241
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04820001T ATE554166T1 (de) | 2003-07-25 | 2004-07-26 | Verfahren und zusammensetzungen zur herstellung von rna aus einer fixierten probe |
Country Status (6)
| Country | Link |
|---|---|
| US (5) | US20050059054A1 (de) |
| EP (2) | EP2295569A1 (de) |
| JP (1) | JP4871722B2 (de) |
| CN (2) | CN1878866B (de) |
| AT (1) | ATE554166T1 (de) |
| WO (1) | WO2005054466A2 (de) |
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| US8076064B2 (en) | 2005-07-09 | 2011-12-13 | Agilent Technologies, Inc. | Method of treatment of RNA sample |
| US7718365B2 (en) | 2005-07-09 | 2010-05-18 | Agilent Technologies, Inc. | Microarray analysis of RNA |
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| US7700289B2 (en) | 2006-04-03 | 2010-04-20 | Agilent Technologies, Inc. | Method for labeling RNA |
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| US10273470B2 (en) | 2010-02-26 | 2019-04-30 | Qiagen Gmbh | Method for isolating RNA from a RNA and DNA containing sample |
| CN101914523B (zh) * | 2010-07-08 | 2013-04-24 | 浙江理工大学 | 从人血清/血浆样本中分离rna方法及其pcr验证法 |
| CN101935648B (zh) * | 2010-09-13 | 2013-04-17 | 原平皓(天津)生物技术有限公司 | 一种提取rna的方法和试剂盒 |
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| EP2505664B1 (de) * | 2011-03-30 | 2014-12-03 | Universität Leipzig | Verfahren und Mittel zur Unterscheidung von gut- und bösartigen Tumorproben, insbesondere bei der FNAB |
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| EP2888354B1 (de) | 2012-08-21 | 2020-04-01 | Qiagen GmbH | Stabilisierung von viruspartikeln und viralen nukleinsäuren in einer biologischen probe |
| EP2888363B1 (de) * | 2012-08-21 | 2018-06-06 | Qiagen GmbH | Verfahren zur isolierung von nukleinsäuren aus einer stabilisierten probe mit formaldehydfreigabe |
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| DE102015017080B3 (de) | 2014-02-28 | 2024-01-04 | Gen-Probe Incorporated | Verfahren zur Isolierung von Nukleinsäure von zytologischen Proben in flüssigen Konservierungsmitteln, die Formaldehyd enthalten |
| FI3670663T3 (fi) | 2014-02-28 | 2024-10-23 | Gen Probe Inc | Menetelmä nukleiinihapon eristämiseksi formaldehydiä sisältävissä nestepohjaisissa sytologisissa säilöntaineissa olevista näytekappaleista |
| US9771572B2 (en) | 2014-02-28 | 2017-09-26 | Gen-Probe Incorporated | Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde |
| CN106574265B (zh) | 2014-07-17 | 2020-07-28 | 凯杰有限公司 | 高产量分离rna的方法 |
| SG10201505563XA (en) * | 2015-07-15 | 2017-02-27 | Delta Electronics Int’L Singapore Pte Ltd | Nucleic acid extraction method |
| CN105063014A (zh) * | 2015-07-28 | 2015-11-18 | 福建师范大学 | 一种利用吸附柱提取植物MicroRNA的方法 |
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| CN111148834B (zh) | 2017-09-27 | 2024-06-11 | 凯杰有限公司 | 以高收率分离rna的方法 |
| CN112522792B (zh) * | 2020-01-22 | 2022-12-20 | 微岩医学科技(北京)有限公司 | 一种rna测序文库的构建方法 |
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| CN114426970A (zh) * | 2022-03-17 | 2022-05-03 | 黑龙江省科学院高技术研究院 | 一种适合杨树rna的提取方法 |
| CN115261368A (zh) * | 2022-08-16 | 2022-11-01 | 北京思尔成生物技术有限公司 | 一种即用型胃蛋白酶工作液及其制备方法和应用 |
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| EP3115470B1 (de) † | 2002-03-13 | 2018-07-18 | Genomic Health, Inc. | Genexpressionsprofilierung in biopsierten tumorgeweben |
| US7364846B2 (en) † | 2002-10-11 | 2008-04-29 | Molecular Devices Corporation | Gene expression profiling from FFPE samples |
| EP1660631B1 (de) * | 2003-08-01 | 2013-04-24 | Life Technologies Corporation | Zusammensetzungen und verfahren zur aufreinigung kurzer rna-moleküle |
| US8312249B1 (en) | 2008-10-10 | 2012-11-13 | Apple Inc. | Dynamic trampoline and structured code generation in a signed code environment |
| FR2957821B1 (fr) | 2010-03-24 | 2014-08-29 | Inst Francais Du Petrole | Nouvelle zone de regeneration du catalyseur divisee en secteurs pour unites catalytiques regeneratives |
-
2004
- 2004-07-26 AT AT04820001T patent/ATE554166T1/de active
- 2004-07-26 WO PCT/US2004/024155 patent/WO2005054466A2/en not_active Ceased
- 2004-07-26 CN CN2004800251339A patent/CN1878866B/zh not_active Expired - Fee Related
- 2004-07-26 CN CN200480025190.7A patent/CN1882688B/zh not_active Expired - Fee Related
- 2004-07-26 EP EP10184549A patent/EP2295569A1/de not_active Ceased
- 2004-07-26 EP EP04820001.8A patent/EP1654361B2/de not_active Expired - Lifetime
- 2004-07-26 US US10/899,386 patent/US20050059054A1/en not_active Abandoned
- 2004-07-26 JP JP2006521996A patent/JP4871722B2/ja not_active Expired - Lifetime
-
2009
- 2009-08-05 US US12/536,073 patent/US20100035770A1/en not_active Abandoned
-
2014
- 2014-03-12 US US14/206,642 patent/US20140329703A1/en not_active Abandoned
-
2016
- 2016-05-23 US US15/162,373 patent/US20160333394A1/en not_active Abandoned
-
2019
- 2019-12-12 US US16/712,102 patent/US20200109440A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20200109440A1 (en) | 2020-04-09 |
| US20100035770A1 (en) | 2010-02-11 |
| EP1654361A2 (de) | 2006-05-10 |
| WO2005054466A3 (en) | 2005-12-15 |
| WO2005054466A2 (en) | 2005-06-16 |
| US20140329703A1 (en) | 2014-11-06 |
| EP1654361B2 (de) | 2023-01-18 |
| CN1882688A (zh) | 2006-12-20 |
| JP4871722B2 (ja) | 2012-02-08 |
| US20050059054A1 (en) | 2005-03-17 |
| EP1654361B1 (de) | 2012-04-18 |
| CN1878866B (zh) | 2012-08-01 |
| JP2007500009A (ja) | 2007-01-11 |
| CN1878866A (zh) | 2006-12-13 |
| CN1882688B (zh) | 2014-07-16 |
| EP2295569A1 (de) | 2011-03-16 |
| US20160333394A1 (en) | 2016-11-17 |
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