ATE440145T1 - Verfahren zur produktion von plasminogen-kringle 2 plus serine protease domäne (k2s) im grossmassstab in prokaryonten - Google Patents

Verfahren zur produktion von plasminogen-kringle 2 plus serine protease domäne (k2s) im grossmassstab in prokaryonten

Info

Publication number
ATE440145T1
ATE440145T1 AT01996619T AT01996619T ATE440145T1 AT E440145 T1 ATE440145 T1 AT E440145T1 AT 01996619 T AT01996619 T AT 01996619T AT 01996619 T AT01996619 T AT 01996619T AT E440145 T1 ATE440145 T1 AT E440145T1
Authority
AT
Austria
Prior art keywords
prokaryonts
kringle
plasminogen
large scale
scale production
Prior art date
Application number
AT01996619T
Other languages
English (en)
Inventor
Rolf-Gunther Werner
Friedrich Goetz
Chatchai Tayapiwatana
Jiradej Manosroi
Aranya Manosroi
Original Assignee
Boehringer Ingelheim Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Int filed Critical Boehringer Ingelheim Int
Application granted granted Critical
Publication of ATE440145T1 publication Critical patent/ATE440145T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
AT01996619T 2000-11-14 2001-11-08 Verfahren zur produktion von plasminogen-kringle 2 plus serine protease domäne (k2s) im grossmassstab in prokaryonten ATE440145T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0027782.2A GB0027782D0 (en) 2000-11-14 2000-11-14 Methods fro large scale protein productio in prokaryotes
PCT/EP2001/012920 WO2002040696A2 (en) 2000-11-14 2001-11-08 Methods for large scale protein production in prokaryotes

Publications (1)

Publication Number Publication Date
ATE440145T1 true ATE440145T1 (de) 2009-09-15

Family

ID=9903155

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01996619T ATE440145T1 (de) 2000-11-14 2001-11-08 Verfahren zur produktion von plasminogen-kringle 2 plus serine protease domäne (k2s) im grossmassstab in prokaryonten

Country Status (18)

Country Link
EP (1) EP1343909B1 (de)
JP (1) JP2004513648A (de)
KR (1) KR100857402B1 (de)
CN (1) CN100457909C (de)
AR (1) AR031336A1 (de)
AT (1) ATE440145T1 (de)
AU (1) AU2002221824A1 (de)
CA (1) CA2428643A1 (de)
CY (1) CY1110546T1 (de)
DE (1) DE60139635D1 (de)
DK (1) DK1343909T3 (de)
ES (1) ES2332124T3 (de)
GB (1) GB0027782D0 (de)
HK (1) HK1077848A1 (de)
MX (1) MXPA03004162A (de)
PT (1) PT1343909E (de)
UY (1) UY27021A1 (de)
WO (1) WO2002040696A2 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0027779D0 (en) * 2000-11-14 2000-12-27 Boehringer Ingelheim Int Methods for large scale production of recombinant dna-derived tpa or k2s molecules
US7087412B2 (en) 2000-11-14 2006-08-08 Boehringer Ingelheim International Gmbh Methods for large scale protein production in prokaryotes
US6955910B2 (en) 2000-11-14 2005-10-18 Boehringer Ingelheim International Gmbh Method for large scale production of recombinant DNA-derived TPA or K2S molecules
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
EP1687324B1 (de) 2003-11-21 2012-08-22 Pfenex, Inc. Verbessertes expressionssystem mit sec-system-sekretion
EP2412816B1 (de) 2004-07-26 2014-12-03 Pfenex Inc. Verfahren zur verbesserten Proteinexpression durch Strain-Engineering
CA2677179C (en) 2007-01-31 2016-02-16 Dow Global Technologies Inc. Bacterial leader sequences for increased expression
US9394571B2 (en) 2007-04-27 2016-07-19 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
CN102770555B (zh) * 2009-11-17 2015-01-28 詹森生物科技公司 改善的细菌膜蛋白分泌
CN102924602B (zh) * 2011-08-08 2015-06-17 深圳市艾派格生物技术有限公司 金离子吸附蛋白表面展示系统及富集并回收金离子的方法
CN106188311B (zh) * 2016-07-18 2020-08-04 山东大学齐鲁医院 一种重组肿瘤坏死因子相关细胞凋亡诱导配体蛋白的制备方法与应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5223407A (en) * 1988-08-31 1993-06-29 Allelix Inc. Excretion of heterologous proteins from e. coli
EP0467676A3 (en) * 1990-07-18 1992-11-19 Applied Immunesciences, Inc. Temperature-inducible secretion expression vectors and their use in prokaryotic cells
WO1997038123A1 (en) * 1996-04-05 1997-10-16 Board Of Regents, The University Of Texas System Methods for producing soluble, biologically-active disulfide bond-containing eukaryotic proteins in bacterial cells
EP1048732A1 (de) * 1999-04-26 2000-11-02 F. Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen

Also Published As

Publication number Publication date
MXPA03004162A (es) 2004-04-20
DE60139635D1 (de) 2009-10-01
UY27021A1 (es) 2002-07-31
EP1343909B1 (de) 2009-08-19
CY1110546T1 (el) 2015-04-29
HK1077848A1 (en) 2006-02-24
JP2004513648A (ja) 2004-05-13
KR20030051821A (ko) 2003-06-25
AR031336A1 (es) 2003-09-17
WO2002040696A2 (en) 2002-05-23
WO2002040696A3 (en) 2002-09-19
GB0027782D0 (en) 2000-12-27
CN1628176A (zh) 2005-06-15
CN100457909C (zh) 2009-02-04
KR100857402B1 (ko) 2008-09-08
CA2428643A1 (en) 2002-05-23
DK1343909T3 (da) 2009-12-21
EP1343909A2 (de) 2003-09-17
PT1343909E (pt) 2009-09-11
AU2002221824A1 (en) 2002-05-27
ES2332124T3 (es) 2010-01-27

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