UY27021A1 - Métodos para la producción a gran escala de proteínas en procariotas - Google Patents
Métodos para la producción a gran escala de proteínas en procariotasInfo
- Publication number
- UY27021A1 UY27021A1 UY27021A UY27021A UY27021A1 UY 27021 A1 UY27021 A1 UY 27021A1 UY 27021 A UY27021 A UY 27021A UY 27021 A UY27021 A UY 27021A UY 27021 A1 UY27021 A1 UY 27021A1
- Authority
- UY
- Uruguay
- Prior art keywords
- methods
- protein
- procariots
- proteins
- scale production
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
El invento pertenece al sector de la producción de proteínas en células procarióticas. El invento se refiere a métodos para la producción de una proteína heterologa que se deriva de un ADN recombinante en células procarióticas, en el que dicha proteína heterologa se segrega extracelularmente como una proteína activa y correctamente plegada, y la célula procariótica contiene y expresa un vector que comprende el ADN que codifica dicha proteína heterologa, unido operativamente con el ADN que codifica el péptido de senal OmpA.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB0027782.2A GB0027782D0 (en) | 2000-11-14 | 2000-11-14 | Methods fro large scale protein productio in prokaryotes |
Publications (1)
Publication Number | Publication Date |
---|---|
UY27021A1 true UY27021A1 (es) | 2002-07-31 |
Family
ID=9903155
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
UY27021A UY27021A1 (es) | 2000-11-14 | 2001-11-12 | Métodos para la producción a gran escala de proteínas en procariotas |
Country Status (18)
Country | Link |
---|---|
EP (1) | EP1343909B1 (es) |
JP (1) | JP2004513648A (es) |
KR (1) | KR100857402B1 (es) |
CN (1) | CN100457909C (es) |
AR (1) | AR031336A1 (es) |
AT (1) | ATE440145T1 (es) |
AU (1) | AU2002221824A1 (es) |
CA (1) | CA2428643A1 (es) |
CY (1) | CY1110546T1 (es) |
DE (1) | DE60139635D1 (es) |
DK (1) | DK1343909T3 (es) |
ES (1) | ES2332124T3 (es) |
GB (1) | GB0027782D0 (es) |
HK (1) | HK1077848A1 (es) |
MX (1) | MXPA03004162A (es) |
PT (1) | PT1343909E (es) |
UY (1) | UY27021A1 (es) |
WO (1) | WO2002040696A2 (es) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7087412B2 (en) | 2000-11-14 | 2006-08-08 | Boehringer Ingelheim International Gmbh | Methods for large scale protein production in prokaryotes |
GB0027779D0 (en) * | 2000-11-14 | 2000-12-27 | Boehringer Ingelheim Int | Methods for large scale production of recombinant dna-derived tpa or k2s molecules |
US6955910B2 (en) | 2000-11-14 | 2005-10-18 | Boehringer Ingelheim International Gmbh | Method for large scale production of recombinant DNA-derived TPA or K2S molecules |
US9453251B2 (en) | 2002-10-08 | 2016-09-27 | Pfenex Inc. | Expression of mammalian proteins in Pseudomonas fluorescens |
EP2336153B1 (en) | 2003-11-21 | 2016-03-30 | Pfenex Inc. | Improved expression systems with SEC-system secretion |
JP2008507294A (ja) | 2004-07-26 | 2008-03-13 | ダウ グローバル テクノロジーズ インコーポレイティド | 菌株遺伝子操作による改善されたタンパク質発現のための方法 |
KR101491867B1 (ko) | 2007-01-31 | 2015-02-10 | 피페넥스 인크. | 증가된 발현을 위한 박테리아 리더 서열 |
US9580719B2 (en) | 2007-04-27 | 2017-02-28 | Pfenex, Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
JP5444553B2 (ja) | 2007-04-27 | 2014-03-19 | フェネックス インコーポレイテッド | 微生物宿主を迅速にスクリーニングして、異種タンパク質発現の収率および/または質が改善されている特定の株を同定する方法 |
CN102770555B (zh) * | 2009-11-17 | 2015-01-28 | 詹森生物科技公司 | 改善的细菌膜蛋白分泌 |
CN102924602B (zh) * | 2011-08-08 | 2015-06-17 | 深圳市艾派格生物技术有限公司 | 金离子吸附蛋白表面展示系统及富集并回收金离子的方法 |
CN106188311B (zh) * | 2016-07-18 | 2020-08-04 | 山东大学齐鲁医院 | 一种重组肿瘤坏死因子相关细胞凋亡诱导配体蛋白的制备方法与应用 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5223407A (en) * | 1988-08-31 | 1993-06-29 | Allelix Inc. | Excretion of heterologous proteins from e. coli |
JPH05219963A (ja) * | 1990-07-18 | 1993-08-31 | Applied Immune Sci Inc | 温度誘導分泌発現ベクターおよびその原核細胞での使用 |
US6027888A (en) * | 1996-04-05 | 2000-02-22 | Board Of Regents, The University Of Texas System | Methods for producing soluble, biologically-active disulfide-bond containing eukaryotic proteins in bacterial cells |
EP1048732A1 (de) * | 1999-04-26 | 2000-11-02 | F. Hoffmann-La Roche Ag | Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen |
-
2000
- 2000-11-14 GB GBGB0027782.2A patent/GB0027782D0/en not_active Ceased
-
2001
- 2001-11-08 DE DE60139635T patent/DE60139635D1/de not_active Expired - Lifetime
- 2001-11-08 MX MXPA03004162A patent/MXPA03004162A/es active IP Right Grant
- 2001-11-08 PT PT01996619T patent/PT1343909E/pt unknown
- 2001-11-08 WO PCT/EP2001/012920 patent/WO2002040696A2/en active Application Filing
- 2001-11-08 DK DK01996619T patent/DK1343909T3/da active
- 2001-11-08 CN CNB018188974A patent/CN100457909C/zh not_active Expired - Fee Related
- 2001-11-08 AT AT01996619T patent/ATE440145T1/de active
- 2001-11-08 ES ES01996619T patent/ES2332124T3/es not_active Expired - Lifetime
- 2001-11-08 JP JP2002543008A patent/JP2004513648A/ja not_active Withdrawn
- 2001-11-08 CA CA002428643A patent/CA2428643A1/en not_active Abandoned
- 2001-11-08 AU AU2002221824A patent/AU2002221824A1/en not_active Abandoned
- 2001-11-08 KR KR1020037006564A patent/KR100857402B1/ko not_active IP Right Cessation
- 2001-11-08 EP EP01996619A patent/EP1343909B1/en not_active Expired - Lifetime
- 2001-11-12 UY UY27021A patent/UY27021A1/es not_active Application Discontinuation
- 2001-11-14 AR ARP010105300A patent/AR031336A1/es unknown
-
2005
- 2005-11-04 HK HK05109852.3A patent/HK1077848A1/xx not_active IP Right Cessation
-
2009
- 2009-11-12 CY CY20091101181T patent/CY1110546T1/el unknown
Also Published As
Publication number | Publication date |
---|---|
EP1343909A2 (en) | 2003-09-17 |
WO2002040696A2 (en) | 2002-05-23 |
ES2332124T3 (es) | 2010-01-27 |
CA2428643A1 (en) | 2002-05-23 |
DE60139635D1 (de) | 2009-10-01 |
JP2004513648A (ja) | 2004-05-13 |
AR031336A1 (es) | 2003-09-17 |
KR100857402B1 (ko) | 2008-09-08 |
DK1343909T3 (da) | 2009-12-21 |
WO2002040696A3 (en) | 2002-09-19 |
CN100457909C (zh) | 2009-02-04 |
KR20030051821A (ko) | 2003-06-25 |
CY1110546T1 (el) | 2015-04-29 |
HK1077848A1 (en) | 2006-02-24 |
GB0027782D0 (en) | 2000-12-27 |
ATE440145T1 (de) | 2009-09-15 |
PT1343909E (pt) | 2009-09-11 |
AU2002221824A1 (en) | 2002-05-27 |
CN1628176A (zh) | 2005-06-15 |
MXPA03004162A (es) | 2004-04-20 |
EP1343909B1 (en) | 2009-08-19 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
109 | Application deemed to be withdrawn |
Effective date: 20150126 |