MXPA02001424A - Produccion de proteinas. - Google Patents
Produccion de proteinas.Info
- Publication number
- MXPA02001424A MXPA02001424A MXPA02001424A MXPA02001424A MXPA02001424A MX PA02001424 A MXPA02001424 A MX PA02001424A MX PA02001424 A MXPA02001424 A MX PA02001424A MX PA02001424 A MXPA02001424 A MX PA02001424A MX PA02001424 A MXPA02001424 A MX PA02001424A
- Authority
- MX
- Mexico
- Prior art keywords
- heterologous polypeptide
- host cell
- fusion protein
- inclusion bodies
- npro
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
- C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
La presente invencion se refiere a un proceso para la produccion recombinante de un polipeptido heterologo deseado con un termino N homogeneo claramente definido en una celula bacteriana hospedera, en donde inicialmente se forma una proteina de fusion que comprende un peptido con la actividad autoproteolitica de una autoproteasa Npro de un pestivirus y el polipeptido heterologo en la forma de cuerpos de inclusion citoplasmicos en la celula hospedera, y luego se aislan los cuerpos de inclusion y se tratan de tal manera que el polipeptido heterologo deseado se disocie autoproteoliticamente mediante la actividad de Npro, a partir de la proteina de fusion.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AT136799 | 1999-08-09 | ||
PCT/EP2000/007643 WO2001011057A1 (en) | 1999-08-09 | 2000-08-07 | Production of proteins |
Publications (1)
Publication Number | Publication Date |
---|---|
MXPA02001424A true MXPA02001424A (es) | 2002-08-12 |
Family
ID=3512357
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MXPA02001424A MXPA02001424A (es) | 1999-08-09 | 2000-08-07 | Produccion de proteinas. |
Country Status (21)
Country | Link |
---|---|
US (2) | US6936455B1 (es) |
EP (1) | EP1200604B1 (es) |
JP (1) | JP4820512B2 (es) |
KR (1) | KR20020026366A (es) |
CN (1) | CN1230543C (es) |
AT (1) | ATE335826T1 (es) |
AU (1) | AU775662B2 (es) |
CA (1) | CA2379571C (es) |
CZ (1) | CZ302366B6 (es) |
DE (1) | DE60029968T2 (es) |
ES (1) | ES2269173T3 (es) |
HK (1) | HK1047128B (es) |
HU (1) | HU230231B1 (es) |
IL (1) | IL147456A0 (es) |
MX (1) | MXPA02001424A (es) |
NO (1) | NO328339B1 (es) |
NZ (1) | NZ516869A (es) |
PL (1) | PL200225B1 (es) |
SK (1) | SK287488B6 (es) |
TR (1) | TR200200253T2 (es) |
WO (1) | WO2001011057A1 (es) |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
PL203708B1 (pl) * | 1999-08-09 | 2009-11-30 | Sandoz Ag | Cząsteczka kwasu nukleinowego kodująca białko fuzyjne, wektor ekspresyjny zawierający tę cząsteczkę oraz komórka gospodarza bakteryjnego zawierająca ten wektor |
ES2453048T3 (es) | 2004-03-30 | 2014-04-03 | Relypsa, Inc. | Polímeros de unión de ión y usos de los mismos |
WO2006113958A2 (en) * | 2005-04-26 | 2006-11-02 | Sandoz Ag | Affinity ligands |
GB0508435D0 (en) * | 2005-04-26 | 2005-06-01 | Sandoz Ag | Organic compounds |
GB0508434D0 (en) * | 2005-04-26 | 2005-06-01 | Sandoz Ag | Organic compounds |
AU2011253661B2 (en) * | 2005-04-26 | 2013-06-13 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Production of recombinant proteins by autoproteolytic cleavage of a fusion protein |
JP5985394B2 (ja) * | 2010-08-31 | 2016-09-06 | 天野エンザイム株式会社 | 酵素を用いた卵殻膜の可溶化方法 |
EP2684951A1 (en) | 2012-07-13 | 2014-01-15 | Sandoz Ag | Method for producing a recombinant protein of interest |
EP2746391A1 (en) | 2012-12-19 | 2014-06-25 | Sandoz Ag | Method for producing a recombinant protein of interest |
EP2746390A1 (en) * | 2012-12-19 | 2014-06-25 | Sandoz Ag | Method for producing a recombinant protein of interest |
UY35874A (es) | 2013-12-12 | 2015-07-31 | Novartis Ag | Un proceso para la preparación de una composición de proteínas pegiladas |
RU2619217C1 (ru) * | 2015-12-04 | 2017-05-12 | Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГБУ "ГосНИИгенетика") | Температурочувствительный мутантный интеин для нерастворимой экспрессии предшественника целевого белка |
CN114539425B (zh) * | 2022-02-25 | 2023-04-28 | 湖南中晟全肽生化有限公司 | 一种提高线性多肽生物表达的方法 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4743679A (en) | 1986-02-24 | 1988-05-10 | Creative Biomolecules, Inc. | Process for producing human epidermal growth factor and analogs thereof |
EP0321973B1 (de) * | 1987-12-23 | 1993-11-03 | BOEHRINGER INGELHEIM INTERNATIONAL GmbH | Expression der viral kodierten Protease P2A des HRV2 |
DE69836075T2 (de) | 1997-04-25 | 2007-06-06 | SemBioSys Genetics, Inc., Calgary | Verfahren zur spaltung von fusionproteinen |
WO1999010483A2 (de) | 1997-08-22 | 1999-03-04 | Roche Diagnostics Gmbh | Autokatalytisch aktivierbare vorstufen von proteasen und deren verwendung |
AU9260598A (en) * | 1997-08-22 | 1999-03-16 | Boehringer Mannheim Gmbh | Zymogenic protease precursors that can be autocatalytically activated and their use |
-
2000
- 2000-08-07 US US10/048,882 patent/US6936455B1/en not_active Expired - Lifetime
- 2000-08-07 IL IL14745600A patent/IL147456A0/xx unknown
- 2000-08-07 JP JP2001515842A patent/JP4820512B2/ja not_active Expired - Lifetime
- 2000-08-07 NZ NZ516869A patent/NZ516869A/en not_active IP Right Cessation
- 2000-08-07 TR TR2002/00253T patent/TR200200253T2/xx unknown
- 2000-08-07 WO PCT/EP2000/007643 patent/WO2001011057A1/en active IP Right Grant
- 2000-08-07 AT AT00958389T patent/ATE335826T1/de active
- 2000-08-07 ES ES00958389T patent/ES2269173T3/es not_active Expired - Lifetime
- 2000-08-07 HU HU0202519A patent/HU230231B1/hu unknown
- 2000-08-07 CA CA2379571A patent/CA2379571C/en not_active Expired - Lifetime
- 2000-08-07 AU AU69933/00A patent/AU775662B2/en not_active Expired
- 2000-08-07 PL PL353101A patent/PL200225B1/pl unknown
- 2000-08-07 KR KR1020027001666A patent/KR20020026366A/ko not_active Application Discontinuation
- 2000-08-07 CZ CZ20020481A patent/CZ302366B6/cs not_active IP Right Cessation
- 2000-08-07 EP EP00958389A patent/EP1200604B1/en not_active Expired - Lifetime
- 2000-08-07 CN CNB008114595A patent/CN1230543C/zh not_active Expired - Lifetime
- 2000-08-07 SK SK185-2002A patent/SK287488B6/sk not_active IP Right Cessation
- 2000-08-07 MX MXPA02001424A patent/MXPA02001424A/es unknown
- 2000-08-07 DE DE60029968T patent/DE60029968T2/de not_active Expired - Lifetime
-
2002
- 2002-02-08 NO NO20020633A patent/NO328339B1/no not_active IP Right Cessation
- 2002-09-25 HK HK02107014.5A patent/HK1047128B/zh not_active IP Right Cessation
-
2005
- 2005-04-05 US US11/099,302 patent/US20050186564A1/en not_active Abandoned
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