TR200200048T2 - Otoproteolitik klevaj vasıtasıyla proteinlerin üretimi. - Google Patents
Otoproteolitik klevaj vasıtasıyla proteinlerin üretimi.Info
- Publication number
- TR200200048T2 TR200200048T2 TR2002/00048T TR200200048T TR200200048T2 TR 200200048 T2 TR200200048 T2 TR 200200048T2 TR 2002/00048 T TR2002/00048 T TR 2002/00048T TR 200200048 T TR200200048 T TR 200200048T TR 200200048 T2 TR200200048 T2 TR 200200048T2
- Authority
- TR
- Turkey
- Prior art keywords
- production
- proteins
- heterologous polypeptide
- autoproteolytic cleavage
- polypeptide
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
- C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Mevcut bulus bir bakteriyel konakçi hücrede açikça tanimli bir homojen N-terminusa sahip arzu edilen bir heterolog polipeptidin üretimi için bir proses ile ilgili olup, içerisinde arzu edilen heterolog polipeptit, Npro otoproteolitik aktivite vasitasiyla heterolog polipeptiti ve bir pestivirüsün bir otoproteaz Npro 'nun otoproteolitik aktivitesine sahip bir polipeptiti içeren bir ilkin ifade edilen füzyon proteinden otoproteolitikal olarak klavlanir. (Sekil yok)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AT136899 | 1999-08-09 |
Publications (1)
Publication Number | Publication Date |
---|---|
TR200200048T2 true TR200200048T2 (tr) | 2002-04-22 |
Family
ID=3512383
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
TR2002/00048T TR200200048T2 (tr) | 1999-08-09 | 2000-08-07 | Otoproteolitik klevaj vasıtasıyla proteinlerin üretimi. |
Country Status (17)
Country | Link |
---|---|
US (1) | US7378512B2 (tr) |
EP (1) | EP1200603B1 (tr) |
JP (1) | JP5480458B2 (tr) |
KR (1) | KR100735791B1 (tr) |
CN (1) | CN1230542C (tr) |
AU (1) | AU775681B2 (tr) |
CA (1) | CA2380302C (tr) |
CZ (1) | CZ303341B6 (tr) |
HK (1) | HK1047127B (tr) |
HU (1) | HU230117B1 (tr) |
IL (2) | IL147411A0 (tr) |
MX (1) | MXPA02001425A (tr) |
NO (1) | NO329246B1 (tr) |
PL (1) | PL203708B1 (tr) |
SK (1) | SK288290B6 (tr) |
TR (1) | TR200200048T2 (tr) |
WO (1) | WO2001011056A1 (tr) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2823220B1 (fr) | 2001-04-04 | 2003-12-12 | Genodyssee | Nouveaux polynucleotides et polypeptides de l'erythropoietine (epo) |
CN100392087C (zh) * | 2001-11-26 | 2008-06-04 | 昆士兰大学 | 黄病毒疫苗输送系统 |
GB0508434D0 (en) * | 2005-04-26 | 2005-06-01 | Sandoz Ag | Organic compounds |
GB0508435D0 (en) * | 2005-04-26 | 2005-06-01 | Sandoz Ag | Organic compounds |
EP2348053A3 (en) * | 2005-04-26 | 2011-11-02 | Sandoz AG | Oligopeptide ligands |
AU2011253661B2 (en) * | 2005-04-26 | 2013-06-13 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Production of recombinant proteins by autoproteolytic cleavage of a fusion protein |
US20100137563A1 (en) * | 2008-12-03 | 2010-06-03 | Northwestern University | Cysteine Protease Autoprocessing of Fusion Proteins |
EP2684951A1 (en) | 2012-07-13 | 2014-01-15 | Sandoz Ag | Method for producing a recombinant protein of interest |
EP2746390A1 (en) * | 2012-12-19 | 2014-06-25 | Sandoz Ag | Method for producing a recombinant protein of interest |
EP2746391A1 (en) * | 2012-12-19 | 2014-06-25 | Sandoz Ag | Method for producing a recombinant protein of interest |
UY35874A (es) | 2013-12-12 | 2015-07-31 | Novartis Ag | Un proceso para la preparación de una composición de proteínas pegiladas |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4743679A (en) * | 1986-02-24 | 1988-05-10 | Creative Biomolecules, Inc. | Process for producing human epidermal growth factor and analogs thereof |
EP0321973B1 (de) * | 1987-12-23 | 1993-11-03 | BOEHRINGER INGELHEIM INTERNATIONAL GmbH | Expression der viral kodierten Protease P2A des HRV2 |
US6077694A (en) * | 1990-09-21 | 2000-06-20 | The United States Of America As Represented By The Department Of Health And Human Services | Method for over-expression and rapid purification of biosynthetic proteins |
ES2273414T3 (es) * | 1997-04-25 | 2007-05-01 | Sembiosys Genetics Inc. | Procedimiento para el corte de proteinas de fusion. |
IL134653A0 (en) | 1997-08-22 | 2001-04-30 | Roche Diagnostics Gmbh | Zymogenic protease precursors that can be autocatalytically activated and their use |
AU9341398A (en) * | 1997-08-22 | 1999-03-16 | Boehringer Mannheim Gmbh | Protease precursors that can be autocatalytically activated and their use |
JP4820512B2 (ja) * | 1999-08-09 | 2011-11-24 | サンド・アクチエンゲゼルシヤフト | タンパク質の製造 |
-
2000
- 2000-08-07 JP JP2001515841A patent/JP5480458B2/ja not_active Expired - Lifetime
- 2000-08-07 TR TR2002/00048T patent/TR200200048T2/tr unknown
- 2000-08-07 CN CNB008110719A patent/CN1230542C/zh not_active Expired - Lifetime
- 2000-08-07 AU AU66998/00A patent/AU775681B2/en not_active Expired
- 2000-08-07 MX MXPA02001425A patent/MXPA02001425A/es active IP Right Grant
- 2000-08-07 SK SK187-2002A patent/SK288290B6/sk not_active IP Right Cessation
- 2000-08-07 IL IL14741100A patent/IL147411A0/xx unknown
- 2000-08-07 HU HU0203051A patent/HU230117B1/hu unknown
- 2000-08-07 KR KR1020027001667A patent/KR100735791B1/ko active IP Right Grant
- 2000-08-07 CZ CZ20020480A patent/CZ303341B6/cs not_active IP Right Cessation
- 2000-08-07 EP EP00954596.3A patent/EP1200603B1/en not_active Expired - Lifetime
- 2000-08-07 CA CA2380302A patent/CA2380302C/en not_active Expired - Lifetime
- 2000-08-07 WO PCT/EP2000/007642 patent/WO2001011056A1/en active IP Right Grant
- 2000-08-07 PL PL353090A patent/PL203708B1/pl unknown
-
2001
- 2001-12-31 IL IL147411A patent/IL147411A/en active IP Right Grant
-
2002
- 2002-01-31 NO NO20020507A patent/NO329246B1/no not_active IP Right Cessation
- 2002-09-24 HK HK02106953.0A patent/HK1047127B/zh not_active IP Right Cessation
-
2004
- 2004-01-23 US US10/763,619 patent/US7378512B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
HUP0203051A2 (hu) | 2002-12-28 |
IL147411A (en) | 2013-12-31 |
NO20020507D0 (no) | 2002-01-31 |
HUP0203051A3 (en) | 2003-12-29 |
HK1047127B (zh) | 2015-08-21 |
JP2003506092A (ja) | 2003-02-18 |
SK288290B6 (sk) | 2015-07-01 |
HU230117B1 (hu) | 2015-08-28 |
CA2380302C (en) | 2013-02-26 |
NO20020507L (no) | 2002-04-05 |
PL203708B1 (pl) | 2009-11-30 |
CN1367837A (zh) | 2002-09-04 |
EP1200603A1 (en) | 2002-05-02 |
EP1200603B1 (en) | 2014-12-24 |
WO2001011056A1 (en) | 2001-02-15 |
HK1047127A1 (en) | 2003-02-07 |
NO329246B1 (no) | 2010-09-20 |
JP5480458B2 (ja) | 2014-04-23 |
AU775681B2 (en) | 2004-08-12 |
KR100735791B1 (ko) | 2007-07-06 |
US7378512B2 (en) | 2008-05-27 |
CZ303341B6 (cs) | 2012-08-08 |
CN1230542C (zh) | 2005-12-07 |
CZ2002480A3 (cs) | 2002-05-15 |
SK1872002A3 (en) | 2002-07-02 |
AU6699800A (en) | 2001-03-05 |
IL147411A0 (en) | 2002-08-14 |
CA2380302A1 (en) | 2001-02-15 |
MXPA02001425A (es) | 2002-08-12 |
KR20020021175A (ko) | 2002-03-18 |
US20040215008A1 (en) | 2004-10-28 |
PL353090A1 (en) | 2003-10-06 |
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