TR200200048T2 - Otoproteolitik klevaj vasıtasıyla proteinlerin üretimi. - Google Patents

Otoproteolitik klevaj vasıtasıyla proteinlerin üretimi.

Info

Publication number
TR200200048T2
TR200200048T2 TR2002/00048T TR200200048T TR200200048T2 TR 200200048 T2 TR200200048 T2 TR 200200048T2 TR 2002/00048 T TR2002/00048 T TR 2002/00048T TR 200200048 T TR200200048 T TR 200200048T TR 200200048 T2 TR200200048 T2 TR 200200048T2
Authority
TR
Turkey
Prior art keywords
production
proteins
heterologous polypeptide
autoproteolytic cleavage
polypeptide
Prior art date
Application number
TR2002/00048T
Other languages
English (en)
Inventor
R�Menapf Tillmann
Thiel Heinz-J�Rgen
Windissch J�Rg
Knauseder Franz
Original Assignee
Biochemie Gesellschaft M.B.H.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biochemie Gesellschaft M.B.H. filed Critical Biochemie Gesellschaft M.B.H.
Publication of TR200200048T2 publication Critical patent/TR200200048T2/tr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Mevcut bulus bir bakteriyel konakçi hücrede açikça tanimli bir homojen N-terminusa sahip arzu edilen bir heterolog polipeptidin üretimi için bir proses ile ilgili olup, içerisinde arzu edilen heterolog polipeptit, Npro otoproteolitik aktivite vasitasiyla heterolog polipeptiti ve bir pestivirüsün bir otoproteaz Npro 'nun otoproteolitik aktivitesine sahip bir polipeptiti içeren bir ilkin ifade edilen füzyon proteinden otoproteolitikal olarak klavlanir. (Sekil yok)
TR2002/00048T 1999-08-09 2000-08-07 Otoproteolitik klevaj vasıtasıyla proteinlerin üretimi. TR200200048T2 (tr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
AT136899 1999-08-09

Publications (1)

Publication Number Publication Date
TR200200048T2 true TR200200048T2 (tr) 2002-04-22

Family

ID=3512383

Family Applications (1)

Application Number Title Priority Date Filing Date
TR2002/00048T TR200200048T2 (tr) 1999-08-09 2000-08-07 Otoproteolitik klevaj vasıtasıyla proteinlerin üretimi.

Country Status (17)

Country Link
US (1) US7378512B2 (tr)
EP (1) EP1200603B1 (tr)
JP (1) JP5480458B2 (tr)
KR (1) KR100735791B1 (tr)
CN (1) CN1230542C (tr)
AU (1) AU775681B2 (tr)
CA (1) CA2380302C (tr)
CZ (1) CZ303341B6 (tr)
HK (1) HK1047127B (tr)
HU (1) HU230117B1 (tr)
IL (2) IL147411A0 (tr)
MX (1) MXPA02001425A (tr)
NO (1) NO329246B1 (tr)
PL (1) PL203708B1 (tr)
SK (1) SK288290B6 (tr)
TR (1) TR200200048T2 (tr)
WO (1) WO2001011056A1 (tr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2823220B1 (fr) 2001-04-04 2003-12-12 Genodyssee Nouveaux polynucleotides et polypeptides de l'erythropoietine (epo)
WO2003046189A1 (en) * 2001-11-26 2003-06-05 The University Of Queensland Flavivirus vaccine delivery system
US7829319B2 (en) * 2005-04-26 2010-11-09 Sandoz Ag Glutamic acid-modified classical swine fever virus autoproteases Npro
GB0508435D0 (en) * 2005-04-26 2005-06-01 Sandoz Ag Organic compounds
GB0508434D0 (en) * 2005-04-26 2005-06-01 Sandoz Ag Organic compounds
AU2011253661B2 (en) * 2005-04-26 2013-06-13 Boehringer Ingelheim Rcv Gmbh & Co Kg Production of recombinant proteins by autoproteolytic cleavage of a fusion protein
US20100137563A1 (en) * 2008-12-03 2010-06-03 Northwestern University Cysteine Protease Autoprocessing of Fusion Proteins
EP2684951A1 (en) 2012-07-13 2014-01-15 Sandoz Ag Method for producing a recombinant protein of interest
EP2746391A1 (en) 2012-12-19 2014-06-25 Sandoz Ag Method for producing a recombinant protein of interest
EP2746390A1 (en) * 2012-12-19 2014-06-25 Sandoz Ag Method for producing a recombinant protein of interest
UY35874A (es) 2013-12-12 2015-07-31 Novartis Ag Un proceso para la preparación de una composición de proteínas pegiladas
CN117362401A (zh) * 2023-09-27 2024-01-09 中国农业科学院兰州兽医研究所 一种牛病毒性腹泻病毒n蛋白及其作为自我切割蛋白的应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4743679A (en) * 1986-02-24 1988-05-10 Creative Biomolecules, Inc. Process for producing human epidermal growth factor and analogs thereof
ES2059482T3 (es) 1987-12-23 1994-11-16 Boehringer Ingelheim Int Expresion de la proteasa p2a de hrv2 codificada por virus.
US6077694A (en) * 1990-09-21 2000-06-20 The United States Of America As Represented By The Department Of Health And Human Services Method for over-expression and rapid purification of biosynthetic proteins
ES2273414T3 (es) * 1997-04-25 2007-05-01 Sembiosys Genetics Inc. Procedimiento para el corte de proteinas de fusion.
JP3947653B2 (ja) 1997-08-22 2007-07-25 ロシュ ダイアグノスティックス ゲーエムベーハー プロテアーゼの自己触媒的に活性化可能なチモーゲン前駆体及びそれらの使用
WO1999010483A2 (de) * 1997-08-22 1999-03-04 Roche Diagnostics Gmbh Autokatalytisch aktivierbare vorstufen von proteasen und deren verwendung
SK287488B6 (sk) * 1999-08-09 2010-11-08 Sandoz Ag Spôsob rekombinantnej produkcie heterológneho polypeptidu pomocou peptidu s autoproteolytickou aktivitou

Also Published As

Publication number Publication date
CN1230542C (zh) 2005-12-07
HK1047127A1 (en) 2003-02-07
SK288290B6 (sk) 2015-07-01
AU775681B2 (en) 2004-08-12
US20040215008A1 (en) 2004-10-28
EP1200603A1 (en) 2002-05-02
CA2380302A1 (en) 2001-02-15
HK1047127B (en) 2015-08-21
IL147411A (en) 2013-12-31
HU230117B1 (hu) 2015-08-28
CZ303341B6 (cs) 2012-08-08
WO2001011056A1 (en) 2001-02-15
CA2380302C (en) 2013-02-26
US7378512B2 (en) 2008-05-27
KR20020021175A (ko) 2002-03-18
NO20020507D0 (no) 2002-01-31
CN1367837A (zh) 2002-09-04
MXPA02001425A (es) 2002-08-12
KR100735791B1 (ko) 2007-07-06
SK1872002A3 (en) 2002-07-02
NO329246B1 (no) 2010-09-20
AU6699800A (en) 2001-03-05
JP2003506092A (ja) 2003-02-18
EP1200603B1 (en) 2014-12-24
JP5480458B2 (ja) 2014-04-23
NO20020507L (no) 2002-04-05
PL203708B1 (pl) 2009-11-30
IL147411A0 (en) 2002-08-14
PL353090A1 (en) 2003-10-06
HUP0203051A3 (en) 2003-12-29
HUP0203051A2 (hu) 2002-12-28
CZ2002480A3 (cs) 2002-05-15

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