WO2000049039A3 - Glucose-dehydrogenase-fusionsproteine und ihre verwendung in expressionssystemen - Google Patents

Glucose-dehydrogenase-fusionsproteine und ihre verwendung in expressionssystemen Download PDF

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Publication number
WO2000049039A3
WO2000049039A3 PCT/EP2000/000978 EP0000978W WO0049039A3 WO 2000049039 A3 WO2000049039 A3 WO 2000049039A3 EP 0000978 W EP0000978 W EP 0000978W WO 0049039 A3 WO0049039 A3 WO 0049039A3
Authority
WO
WIPO (PCT)
Prior art keywords
utilization
expression systems
fusion proteins
glucose dehydrogenase
dehydrogenase fusion
Prior art date
Application number
PCT/EP2000/000978
Other languages
English (en)
French (fr)
Other versions
WO2000049039A2 (de
Inventor
Winfried Linxweiler
Christa Burger
Oliver Poeschke
Uwe Hofmann
Andrea Wolf
Original Assignee
Merck Patent Gmbh
Winfried Linxweiler
Christa Burger
Oliver Poeschke
Uwe Hofmann
Andrea Wolf
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent Gmbh, Winfried Linxweiler, Christa Burger, Oliver Poeschke, Uwe Hofmann, Andrea Wolf filed Critical Merck Patent Gmbh
Priority to BR0008370-4A priority Critical patent/BR0008370A/pt
Priority to JP2000599776A priority patent/JP2002538782A/ja
Priority to CA002368461A priority patent/CA2368461A1/en
Priority to AU25468/00A priority patent/AU771320B2/en
Priority to SK1174-2001A priority patent/SK11742001A3/sk
Priority to KR1020017010567A priority patent/KR20010103017A/ko
Priority to EP00903672A priority patent/EP1155130A2/de
Publication of WO2000049039A2 publication Critical patent/WO2000049039A2/de
Publication of WO2000049039A3 publication Critical patent/WO2000049039A3/de
Priority to NO20014011A priority patent/NO20014011L/no
Priority to US10/681,207 priority patent/US20050112744A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Die Erfindung betrifft neue rekombinante Fusionsproteine, welche als ein Bestandteil eine Proteinsequenz mit der biologischen Aktivität von Glucose-Dehydrogenase enthalten sowie ihre Verwendung zum einfachen und effizienten Nachweis von beliebigen Proteinen/Polypeptiden in SDS-PAGE-Gelen und zur raschen Optimierung von Expressionsystemen, welche besagte Proteine/Polypeptide zu exprimieren in der Lage sind.
PCT/EP2000/000978 1999-02-19 2000-02-08 Glucose-dehydrogenase-fusionsproteine und ihre verwendung in expressionssystemen WO2000049039A2 (de)

Priority Applications (9)

Application Number Priority Date Filing Date Title
BR0008370-4A BR0008370A (pt) 1999-02-19 2000-02-08 Proteìnas de fusão de glicose deshidrogenase e uso das mesmas em sistemas de expressão
JP2000599776A JP2002538782A (ja) 1999-02-19 2000-02-08 グルコースデヒドロゲナーゼ融合蛋白質及び発現システムにおけるそれらの使用
CA002368461A CA2368461A1 (en) 1999-02-19 2000-02-08 Glucose dehydrogenase fusion proteins and their utilization in expression systems
AU25468/00A AU771320B2 (en) 1999-02-19 2000-02-08 Glucose dehydrogenase fusion proteins and their utilization in expression systems
SK1174-2001A SK11742001A3 (sk) 1999-02-19 2000-02-08 Glukózové dehydrogenázové fúzne proteíny a ich použitie v expresných systémoch
KR1020017010567A KR20010103017A (ko) 1999-02-19 2000-02-08 글루코스 데히드로게나제 융합 단백질 및 발현계에서의그의 용도
EP00903672A EP1155130A2 (de) 1999-02-19 2000-02-08 Glucose-dehydrogenase-fusionsproteine und ihre verwendung in expressionssystemen
NO20014011A NO20014011L (no) 1999-02-19 2001-08-17 Glukosedehydrogenasefusjonsproteiner og deres anvendelse i ekspresjonssystemer
US10/681,207 US20050112744A1 (en) 1999-02-19 2003-10-09 Glucose dehydrogenase fusion proteins and their use in expression systems

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19906920.4 1999-02-19
DE19906920 1999-02-19

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/681,207 Division US20050112744A1 (en) 1999-02-19 2003-10-09 Glucose dehydrogenase fusion proteins and their use in expression systems

Publications (2)

Publication Number Publication Date
WO2000049039A2 WO2000049039A2 (de) 2000-08-24
WO2000049039A3 true WO2000049039A3 (de) 2000-12-14

Family

ID=7897979

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/000978 WO2000049039A2 (de) 1999-02-19 2000-02-08 Glucose-dehydrogenase-fusionsproteine und ihre verwendung in expressionssystemen

Country Status (16)

Country Link
US (1) US20050112744A1 (de)
EP (1) EP1155130A2 (de)
JP (1) JP2002538782A (de)
KR (1) KR20010103017A (de)
CN (1) CN1340104A (de)
AR (1) AR022630A1 (de)
AU (1) AU771320B2 (de)
BR (1) BR0008370A (de)
CA (1) CA2368461A1 (de)
CZ (1) CZ20012739A3 (de)
HU (1) HUP0200285A2 (de)
NO (1) NO20014011L (de)
PL (1) PL350574A1 (de)
SK (1) SK11742001A3 (de)
WO (1) WO2000049039A2 (de)
ZA (1) ZA200107686B (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE390481T1 (de) * 2001-12-21 2008-04-15 Curacyte Ag Modifizierte tridegine, ihre herstellung und verwendung als transglutaminase-inhibitoren
MXPA06001719A (es) 2003-08-11 2006-05-19 Codexis Inc Polipeptidos de glucosa deshidrogenasa mejorados, y polinucleotidos relacionados.
HUE032804T2 (en) * 2006-04-13 2017-11-28 Hoffmann La Roche Enhanced mutants of pyrroloquinoline-quinone-dependent soluble glucose dehydrogenase
CN110894504A (zh) * 2019-12-20 2020-03-20 武汉茵慕生物科技有限公司 强化表达葡萄糖6-磷酸脱氢酶的地衣芽胞杆菌在异源蛋白生产中的应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63230098A (ja) * 1987-03-18 1988-09-26 Fujitsu Ltd 酵素の分析方法
EP0290768B1 (de) * 1987-04-08 1994-01-19 MERCK PATENT GmbH Verfahren zur Herstellung von Glucosedehydrogenase aus Bacillus megaterium
WO1998035025A1 (fr) * 1997-02-07 1998-08-13 Kaneka Corporation Nouvelle carbonyle reductase, gene codant celle-ci et procede d'utilisation

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60141299A (ja) * 1983-12-28 1985-07-26 Wako Pure Chem Ind Ltd 脱水素酵素の活性度測定法
US6399859B1 (en) * 1997-12-10 2002-06-04 Pioneer Hi-Bred International, Inc. Plant uridine diphosphate-glucose dehydrogenase genes, proteins, and uses thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63230098A (ja) * 1987-03-18 1988-09-26 Fujitsu Ltd 酵素の分析方法
EP0290768B1 (de) * 1987-04-08 1994-01-19 MERCK PATENT GmbH Verfahren zur Herstellung von Glucosedehydrogenase aus Bacillus megaterium
WO1998035025A1 (fr) * 1997-02-07 1998-08-13 Kaneka Corporation Nouvelle carbonyle reductase, gene codant celle-ci et procede d'utilisation
EP0967271A1 (de) * 1997-02-07 1999-12-29 Kaneka Corporation Neue carbonyl-reduktase, gene welche diese kodieren und methoden zu deren verwendung

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Section Ch Week 198844, Derwent World Patents Index; Class D16, AN 1988-311973, XP002144192 *
DATABASE WPI Section Ch Week 199838, Derwent World Patents Index; Class B04, AN 1998-447222, XP002144211 *
FUJITA Y ET AL: "LOCATION AND PROPERTIES OF GLUCOSE DEHYDROGENASE EC-1.1.1.47 IN SPORULATING CELLS AND SPORES OF BACILLUS-SUBTILIS", JOURNAL OF BACTERIOLOGY, vol. 132, no. 1, 1977, EN, pages 282 - 293, XP000933845, ISSN: 0021-9193 *
HOPP T P ET AL: "A SHORT POLYPEPTIDE MARKER SEQUENCE USEFUL FOR RECOMBINANT PROTEIN IDENTIFICATION AND PURIFICATION", BIO/TECHNOLOGY, vol. 6, 1 October 1988 (1988-10-01), pages 1204 - 1210, XP002046115 *
NAGAO T ET AL: "CLONING NUCLEOTIDE SEQUENCES AND ENZYMATIC PROPERTIES OF GLUCOSE DEHYDROGENASE ISOZYMES FROM BACILLUS-MEGATERIUM IAM1030", JOURNAL OF BACTERIOLOGY, vol. 174, no. 15, 1992, pages 5013 - 5020, XP000933848, ISSN: 0021-9193 *
RYGUS T ET AL: "INDUCIBLE HIGH-LEVEL EXPRESSION OF HETEROLOGOUS GENES IN BACILLUS-MEGATERIUM USING THE REGULATORY ELEMENTS OF THE XYLOSE-UTILIZATION OPERON", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 35, no. 5, 1991, pages 594 - 599, XP000933839, ISSN: 0175-7598 *
SINHA P ET AL: "USE OF PHYSIOLOGICAL SUBSTRATES FOR ZYMOGRAMS OF DISACCHARIDASES AFTER SEPARATION IN IMMOBILIZED PH GRADIENTS", JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, vol. 18, no. 3, 1989, pages 195 - 208, XP000933838, ISSN: 0165-022X *

Also Published As

Publication number Publication date
ZA200107686B (en) 2002-12-18
AU2546800A (en) 2000-09-04
AU771320B2 (en) 2004-03-18
JP2002538782A (ja) 2002-11-19
NO20014011D0 (no) 2001-08-17
CA2368461A1 (en) 2000-08-24
CN1340104A (zh) 2002-03-13
EP1155130A2 (de) 2001-11-21
US20050112744A1 (en) 2005-05-26
NO20014011L (no) 2001-10-02
KR20010103017A (ko) 2001-11-17
HUP0200285A2 (hu) 2002-05-29
BR0008370A (pt) 2001-11-06
WO2000049039A2 (de) 2000-08-24
CZ20012739A3 (cs) 2001-11-14
SK11742001A3 (sk) 2002-03-05
PL350574A1 (en) 2002-12-30
AR022630A1 (es) 2002-09-04

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