AR022630A1 - Proteina de fusion recombinante con glucosa-deshidrogenasa; dna que condifica para esta proteina de fusion; un vector de expresion que contiene este dna;la celula huesped que contiene este vector de expresion; el empleo de la glucosa deshidrogenasa para detectar una proteina/un polipeptido recombina - Google Patents
Proteina de fusion recombinante con glucosa-deshidrogenasa; dna que condifica para esta proteina de fusion; un vector de expresion que contiene este dna;la celula huesped que contiene este vector de expresion; el empleo de la glucosa deshidrogenasa para detectar una proteina/un polipeptido recombinaInfo
- Publication number
- AR022630A1 AR022630A1 ARP000100695A ARP000100695A AR022630A1 AR 022630 A1 AR022630 A1 AR 022630A1 AR P000100695 A ARP000100695 A AR P000100695A AR P000100695 A ARP000100695 A AR P000100695A AR 022630 A1 AR022630 A1 AR 022630A1
- Authority
- AR
- Argentina
- Prior art keywords
- protein
- recombinant
- polypeptide
- fusion protein
- expression vector
- Prior art date
Links
- 229920001184 polypeptide Polymers 0.000 title abstract 9
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 9
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 9
- 108010050375 Glucose 1-Dehydrogenase Proteins 0.000 title abstract 5
- 239000013604 expression vector Substances 0.000 title abstract 5
- 108020001507 fusion proteins Proteins 0.000 title abstract 5
- 102000004169 proteins and genes Human genes 0.000 title abstract 5
- 108090000623 proteins and genes Proteins 0.000 title abstract 5
- 238000004064 recycling Methods 0.000 title 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 6
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 6
- 238000001514 detection method Methods 0.000 abstract 3
- 102000037865 fusion proteins Human genes 0.000 abstract 3
- 101710088194 Dehydrogenase Proteins 0.000 abstract 2
- 230000004071 biological effect Effects 0.000 abstract 2
- 230000002255 enzymatic effect Effects 0.000 abstract 2
- 239000000499 gel Substances 0.000 abstract 2
- 238000001502 gel electrophoresis Methods 0.000 abstract 2
- 239000003550 marker Substances 0.000 abstract 2
- 238000002360 preparation method Methods 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 101710086987 X protein Proteins 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 238000005457 optimization Methods 0.000 abstract 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2799/00—Uses of viruses
- C12N2799/02—Uses of viruses as vector
- C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Gastroenterology & Hepatology (AREA)
- General Engineering & Computer Science (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Una proteína de fusion recombinante compuesta por al menos una primera y una segunda secuencia de aminoácidos, en donde la primera secuencia presenta laactividad biologica de la glucosa deshidrogenasa. En particular, la segunda secuencia es una proteína/un polipéptido X recombinante cualquiera o presentapartes de la/el misma/o. La proteína de fusion también puede presentar adicionalmente al menos una secuencia de reconocimiento diferente (secuencia-Tag) quesea adecuada para la deteccion. El DNAque codifica para una proteína de fusion como la descripta más arriba, el vector de expresion que contiene dicho DNA yla célula huésped para la expresion de proteínas/polipéptidos recombinantes que contiene dicho vector de expresion. El empleo de laglucosa deshidrogenasa parala determinacion simple y eficiente de una proteína/polipéptido recombinante cualquiera en geles de SDS-PAGE y para la optimacion rápida de sistemas deexpresion que pueden expresar la proteína/el polipéptido mencionado. Elempleo de un vector de expresion como el mencionado más arriba para optimar laexpresion de una proteína/polipéptido X recombinante en un procedimiento recombinante de preparacion. El empleo de una célula huésped como la mencionada másarriba para optimar la expresion de una proteína/un polipéptido X recombinante en un procedimiento recombinante de preparacion. Un procedimiento paradeterminar rápidamente una proteína/un polipéptido recombinante cualquiera por medio de la electroforesis en gel, enel cual se prepara una proteína de fusioncomo la mencionada más arriba, se la separa por medio de la electroforesis en gel y se visualiza la proteína/el polipéptido recombinante a ser identificada/oen el gel a través de la actividad enzimática de laglucosa deshidrogenasa. En este caso la GlcDH, o la secuencia que exhibe la actividad biologica de la GlcDHadopta el papel de una proteína marcadora o de deteccion. Esta enzima tiene la particularidad de poseer una estabilidad excepcional frente a los agentesdesnaturalizantes tales como el SDS. La GlcDH misma no exhibe disminucion alguna en su actividad enzimática cuando actua como proteína marcadora o de deteccion
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19906920 | 1999-02-19 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR022630A1 true AR022630A1 (es) | 2002-09-04 |
Family
ID=7897979
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP000100695A AR022630A1 (es) | 1999-02-19 | 2000-02-18 | Proteina de fusion recombinante con glucosa-deshidrogenasa; dna que condifica para esta proteina de fusion; un vector de expresion que contiene este dna;la celula huesped que contiene este vector de expresion; el empleo de la glucosa deshidrogenasa para detectar una proteina/un polipeptido recombina |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US20050112744A1 (es) |
| EP (1) | EP1155130A2 (es) |
| JP (1) | JP2002538782A (es) |
| KR (1) | KR20010103017A (es) |
| CN (1) | CN1340104A (es) |
| AR (1) | AR022630A1 (es) |
| AU (1) | AU771320B2 (es) |
| BR (1) | BR0008370A (es) |
| CA (1) | CA2368461A1 (es) |
| CZ (1) | CZ20012739A3 (es) |
| HU (1) | HUP0200285A2 (es) |
| NO (1) | NO20014011L (es) |
| PL (1) | PL350574A1 (es) |
| SK (1) | SK11742001A3 (es) |
| WO (1) | WO2000049039A2 (es) |
| ZA (1) | ZA200107686B (es) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE390481T1 (de) * | 2001-12-21 | 2008-04-15 | Curacyte Ag | Modifizierte tridegine, ihre herstellung und verwendung als transglutaminase-inhibitoren |
| CA2535147A1 (en) | 2003-08-11 | 2005-05-19 | Codexis, Inc. | Improved glucose dehydrogenase polypeptides and related polynucleotides |
| CN101421396B (zh) * | 2006-04-13 | 2016-01-20 | 霍夫曼-拉罗奇有限公司 | 吡咯并喹啉醌依赖性可溶性葡萄糖脱氢酶的改良突变体 |
| CN110894504A (zh) * | 2019-12-20 | 2020-03-20 | 武汉茵慕生物科技有限公司 | 强化表达葡萄糖6-磷酸脱氢酶的地衣芽胞杆菌在异源蛋白生产中的应用 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60141299A (ja) * | 1983-12-28 | 1985-07-26 | Wako Pure Chem Ind Ltd | 脱水素酵素の活性度測定法 |
| JPS63230098A (ja) * | 1987-03-18 | 1988-09-26 | Fujitsu Ltd | 酵素の分析方法 |
| DE3711881A1 (de) * | 1987-04-08 | 1988-10-27 | Merck Patent Gmbh | Verfahren zur herstellung von glucosedehydrogenase aus bacillus megaterium |
| AU4032997A (en) * | 1997-02-07 | 1998-08-26 | Kaneka Corporation | Novel carbonyl reductase, gene that encodes the same, and method of utilizing these |
| US6399859B1 (en) * | 1997-12-10 | 2002-06-04 | Pioneer Hi-Bred International, Inc. | Plant uridine diphosphate-glucose dehydrogenase genes, proteins, and uses thereof |
-
2000
- 2000-02-08 HU HU0200285A patent/HUP0200285A2/hu unknown
- 2000-02-08 SK SK1174-2001A patent/SK11742001A3/sk unknown
- 2000-02-08 AU AU25468/00A patent/AU771320B2/en not_active Ceased
- 2000-02-08 EP EP00903672A patent/EP1155130A2/de not_active Withdrawn
- 2000-02-08 PL PL00350574A patent/PL350574A1/xx unknown
- 2000-02-08 CN CN00803879A patent/CN1340104A/zh active Pending
- 2000-02-08 CZ CZ20012739A patent/CZ20012739A3/cs unknown
- 2000-02-08 JP JP2000599776A patent/JP2002538782A/ja active Pending
- 2000-02-08 WO PCT/EP2000/000978 patent/WO2000049039A2/de not_active Application Discontinuation
- 2000-02-08 BR BR0008370-4A patent/BR0008370A/pt not_active Application Discontinuation
- 2000-02-08 KR KR1020017010567A patent/KR20010103017A/ko not_active Application Discontinuation
- 2000-02-08 CA CA002368461A patent/CA2368461A1/en not_active Abandoned
- 2000-02-18 AR ARP000100695A patent/AR022630A1/es unknown
-
2001
- 2001-08-17 NO NO20014011A patent/NO20014011L/no not_active Application Discontinuation
- 2001-09-18 ZA ZA200107686A patent/ZA200107686B/en unknown
-
2003
- 2003-10-09 US US10/681,207 patent/US20050112744A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2000049039A3 (de) | 2000-12-14 |
| AU771320B2 (en) | 2004-03-18 |
| CN1340104A (zh) | 2002-03-13 |
| CZ20012739A3 (cs) | 2001-11-14 |
| HUP0200285A2 (hu) | 2002-05-29 |
| ZA200107686B (en) | 2002-12-18 |
| JP2002538782A (ja) | 2002-11-19 |
| NO20014011L (no) | 2001-10-02 |
| PL350574A1 (en) | 2002-12-30 |
| NO20014011D0 (no) | 2001-08-17 |
| AU2546800A (en) | 2000-09-04 |
| CA2368461A1 (en) | 2000-08-24 |
| BR0008370A (pt) | 2001-11-06 |
| US20050112744A1 (en) | 2005-05-26 |
| KR20010103017A (ko) | 2001-11-17 |
| EP1155130A2 (de) | 2001-11-21 |
| WO2000049039A2 (de) | 2000-08-24 |
| SK11742001A3 (sk) | 2002-03-05 |
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