KR960010863A - 재조합 머사시딘 및 이의 제조방법 - Google Patents

재조합 머사시딘 및 이의 제조방법 Download PDF

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Publication number
KR960010863A
KR960010863A KR1019950029599A KR19950029599A KR960010863A KR 960010863 A KR960010863 A KR 960010863A KR 1019950029599 A KR1019950029599 A KR 1019950029599A KR 19950029599 A KR19950029599 A KR 19950029599A KR 960010863 A KR960010863 A KR 960010863A
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KR
South Korea
Prior art keywords
premersacidin
acid sequence
amino acid
dna encoding
preparation
Prior art date
Application number
KR1019950029599A
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English (en)
Other versions
KR100394289B1 (ko
Inventor
콜러 클라우스-페터
게오르그 샬 한스
비르바움 가브릴레
Original Assignee
차우너, 루츠
훽스트 아크티엔게젤샤프트
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by 차우너, 루츠, 훽스트 아크티엔게젤샤프트 filed Critical 차우너, 루츠
Publication of KR960010863A publication Critical patent/KR960010863A/ko
Application granted granted Critical
Publication of KR100394289B1 publication Critical patent/KR100394289B1/ko

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/50—Cyclic peptides containing at least one abnormal peptide link
    • C07K7/54—Cyclic peptides containing at least one abnormal peptide link with at least one abnormal peptide link in the ring
    • C07K7/56—Cyclic peptides containing at least one abnormal peptide link with at least one abnormal peptide link in the ring the cyclisation not occurring through 2,4-diamino-butanoic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)

Abstract

본 발명은 특히 펩타이드 항생물질 머사시딘의 구조적 유전자 서열에 관한 것이다. 이의 서열분석은 프레머사시딘이 특수한 48개 아미노산의 길이의 리더 서열과 생합성 과정중 성숙한 란티바이오틱으로 변형되는 20개 아미노산의 프로펩타이드 부분으로 이루어져 있음을 나타낸다.

Description

재조합 머사시딘 및 이의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (10)

  1. 제2도에 나타낸 바와 같은 제1번 재지 제68번의 아미노산 서열을 지닌 프레머사시딘(premersacidin).
  2. 제2도에 나타낸 바와 같은 제49번 내지 제68번의 아미노산 서열을 지닌 프로머사시딘(promersacidin).
  3. 제1항에 따른 프레머사시딘을 암호화하는 DNA.
  4. 제2도에 나타낸 바와 같은 제22번 내지 제225번의 핵산 서열을 지닌 프레머사시딘을 암호화하는 DNA.
  5. 제2항에 따른 프로머사시딘을 암호화하는 DNA.
  6. 제2도에 나타낸 바와 같은 제166번 내지 제225번의 핵산 서열을 지닌 프로머사시딘을 암호화하는 DNA.
  7. 제3항 재지 제6항 중 어느 한 항에 따른 DNA를 포함하는 벡터.
  8. 제7항에 따른 벡터를 포함하는 숙주 세포.
  9. (a)제3항 재지 제6항 중 어느 한 항에 따른 DNA 서열을 포함하는 적합한 숙주 세포를 적합한 조건하에서 배양하는 단계; 및 (b) 프레머사시딘, 프로머사시딘 또는 성숙한 머사시딘을 분리하는 단계를 포함하는, 프레머사시딘, 프로머사시딘 또는 성숙한 머사시딘의 제조방법.
  10. 성숙한 머사시디늘 제조하기 위한 제1항 또는 제2항에 따른 프레머사시딘 또는 프로머사시딘의 용도.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019950029599A 1994-09-12 1995-09-12 재조합 머사시딘 및 이의 제조방법 KR100394289B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP94114298A EP0700998B1 (en) 1994-09-12 1994-09-12 Recombinant mersacidin and a method for production
EP94114298.6 1994-09-12

Publications (2)

Publication Number Publication Date
KR960010863A true KR960010863A (ko) 1996-04-20
KR100394289B1 KR100394289B1 (ko) 2003-10-22

Family

ID=8216276

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019950029599A KR100394289B1 (ko) 1994-09-12 1995-09-12 재조합 머사시딘 및 이의 제조방법

Country Status (19)

Country Link
US (1) US5667991A (ko)
EP (1) EP0700998B1 (ko)
JP (1) JPH0898695A (ko)
KR (1) KR100394289B1 (ko)
CN (1) CN1149287C (ko)
AT (1) ATE255165T1 (ko)
AU (1) AU696450B2 (ko)
CA (1) CA2157975C (ko)
CZ (1) CZ285741B6 (ko)
DE (1) DE69433357T2 (ko)
DK (1) DK0700998T3 (ko)
ES (1) ES2207636T3 (ko)
FI (1) FI120100B (ko)
HU (1) HU216619B (ko)
IL (1) IL115242A0 (ko)
NZ (1) NZ272960A (ko)
PT (1) PT700998E (ko)
RU (1) RU2198924C2 (ko)
SI (1) SI9500270B (ko)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1694020B1 (en) * 1999-03-12 2008-05-28 Aware, Inc. Multicarrier modulation system and method
US6775320B1 (en) 1999-03-12 2004-08-10 Aware, Inc. Method and a multi-carrier transceiver supporting dynamic switching between active application sets
US6861236B2 (en) 2002-05-24 2005-03-01 Applied Nanosystems B.V. Export and modification of (poly)peptides in the lantibiotic way
GB0406870D0 (en) * 2004-03-26 2004-04-28 Novacta Biosystems Ltd Improvements relating to the production of lantibiotics
US7592308B2 (en) 2004-03-26 2009-09-22 Novacta Biosystems Limited F3W variants of the lantibiotic mersacidin and its use
JP2009509519A (ja) * 2005-09-27 2009-03-12 ノヴァクタ バイオシステムズ リミティッド ランチビオティックメルサシジンの変種およびそれらの使用
GB0600928D0 (en) 2006-01-17 2006-02-22 Novacta Biosystems Ltd Improvements relating to lantibiotics
GB0714029D0 (en) 2007-07-18 2007-08-29 Novacta Biosystems Ltd Lantibiotic-based compounds having antimicrobial activity
GB0714030D0 (en) 2007-07-18 2007-08-29 Novacta Biosystems Ltd The use of type-B lantibiotic-based compounds having antimicrobial activity
CN102046652B (zh) * 2008-04-02 2014-11-05 赛诺菲-安万特 来自Actinomadura namibiensis的高度桥联的肽
WO2010058238A1 (en) * 2008-11-24 2010-05-27 Sentinella Pharmaceuticals, Inc. ("Sentinella") Lantibiotic carboxyamide derivatives with enhanced antibacterial activity
US8283371B2 (en) 2009-01-14 2012-10-09 Novacta Biosystems Limited Compounds
EP2393829A1 (en) 2009-02-04 2011-12-14 Novacta Biosystems Limited Actagardine derivatives
GB201001688D0 (en) 2010-02-02 2010-03-17 Novacta Biosystems Ltd Compounds
GB201013513D0 (en) 2010-08-11 2010-09-22 Novacta Biosystems Ltd Formulations
CN106188253B (zh) * 2016-08-26 2020-08-18 上海交通大学 抗菌肽Lexapeptide及其制备方法和用途
EP3921424A1 (en) 2019-02-05 2021-12-15 Elanco Us Inc. A genetically modified lactobacillus and uses thereof
CN111235119B (zh) * 2020-03-05 2021-11-23 苏州十一方生物科技有限公司 一种融合抗菌蛋白的制备及应用
CN114457102B (zh) * 2022-02-24 2023-12-26 重庆市畜牧科学院 用于编码分泌型Mersacidin的基因表达盒及其制备方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5218101A (en) * 1988-07-05 1993-06-08 The University Of Maryland Leader sequence inducing a post-translational modification of polypeptides in bacteria, and gene therefor
IN167138B (ko) * 1988-08-17 1990-09-01 Hoechst India

Also Published As

Publication number Publication date
KR100394289B1 (ko) 2003-10-22
PT700998E (pt) 2004-03-31
DE69433357T2 (de) 2004-09-09
HU9502643D0 (en) 1995-11-28
JPH0898695A (ja) 1996-04-16
CA2157975A1 (en) 1996-03-13
SI9500270A (en) 1996-04-30
CZ285741B6 (cs) 1999-10-13
AU3055495A (en) 1996-03-28
RU2198924C2 (ru) 2003-02-20
HU216619B (hu) 1999-07-28
CZ231895A3 (en) 1996-03-13
CA2157975C (en) 2008-11-18
CN1131192A (zh) 1996-09-18
US5667991A (en) 1997-09-16
SI9500270B (sl) 2002-02-28
DE69433357D1 (de) 2004-01-08
ATE255165T1 (de) 2003-12-15
CN1149287C (zh) 2004-05-12
EP0700998B1 (en) 2003-11-26
FI954220A0 (fi) 1995-09-08
FI120100B (fi) 2009-06-30
FI954220A (fi) 1996-03-13
IL115242A0 (en) 1995-12-31
ES2207636T3 (es) 2004-06-01
AU696450B2 (en) 1998-09-10
EP0700998A1 (en) 1996-03-13
DK0700998T3 (da) 2004-03-29
HUT73800A (en) 1996-09-30
NZ272960A (en) 1996-05-28

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