KR860008282A - 재조합 사람 레닌의 제조방법 - Google Patents
재조합 사람 레닌의 제조방법 Download PDFInfo
- Publication number
- KR860008282A KR860008282A KR1019860002529A KR860002529A KR860008282A KR 860008282 A KR860008282 A KR 860008282A KR 1019860002529 A KR1019860002529 A KR 1019860002529A KR 860002529 A KR860002529 A KR 860002529A KR 860008282 A KR860008282 A KR 860008282A
- Authority
- KR
- South Korea
- Prior art keywords
- renin
- prorenin
- chromatography
- expression
- recombinant host
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6478—Aspartic endopeptidases (3.4.23)
- C12N9/6486—Renin (3.4.23.15)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/23—Aspartic endopeptidases (3.4.23)
- C12Y304/23015—Renin (3.4.23.15)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 전프로레닌 암호화 DNA의 완전한 누클레오티드 서열 및 추론되는 아미노산 서열이고,
제2도는 전프로레닌을 암호화하고, 포유류 세포 배양물로부터 프로레닌의 분비를 초래하는 발현 벡터의 구성 이며,
제3a도 및 제3b도는 레닌-관련 단백질용 세균성 발현 벡터의 구성이고,
제6도는 AtT20세포의 배지로부터 항-레닌으로 면역 침전된35S-메티오닌 표지 단백질의 SDS-폴리아크릴아마이드 겔 이다.
Claims (7)
- 재조합 숙주 세포에 부합하고, 전기 재조합 숙주세포에서 후술하는 DNA서열의 발현을 수행할 수 있는 조정서열(Contr01 Sequence)에 실시 가능하게 연결된 사람레닌, 프로레닌, 또는 전프로레닌(prepro renin)을 암호화하는 DNA서열로 이루어진 발현 시스템.
- 제1항에 있어서, 조정 서열이 포유류 숙주와 부합하는 시스템.
- 제1항에 있어서, 조정 서열이 세균성 숙주와 부합하는 시스템.
- 레닌, 프로레닌, 또는 전프로레닌을 암호화하는 재조합 DNA서열로 형질전환된 재조합 숙주 세포를 배양하고, 배양물로부터 레닌 또는 프로레닌을 회수함을 특징으로 하여, 사람 레닌 또는 프로레닌을 제조하는 방법.
- 본래 조절 분비 회로를 사용하는 포유류 세포를 처리하여, 전프로레닌용 발현 시스템을 함유하도록 변형시키고, 분비 촉진제로 전프로레닌 유전자를 발현하도록 유도시킴을 특징으로 하여, 분비, 배양된 완전재조합 레닌을 제조하는 방법.
- 소수성 상호작용 크로마토그라피(HIC), 펩스타틴(pepstatin) 크로마토그라피, 및 음이온 교환으로 이루어진, 세포 배양 상등액으로부터 레닌을 정제하는 방법.
- 펩스타틴 크로마토그라피, 크기별 제외(size exclusion), 및 음이온 교환으로 이루어진, 세포 배양 상등액으로부터 프로레닌을 정제하는 방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US71941485A | 1985-04-03 | 1985-04-03 | |
US719414 | 1985-04-03 |
Publications (1)
Publication Number | Publication Date |
---|---|
KR860008282A true KR860008282A (ko) | 1986-11-14 |
Family
ID=24889982
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019860002529A KR860008282A (ko) | 1985-04-03 | 1986-04-03 | 재조합 사람 레닌의 제조방법 |
Country Status (13)
Country | Link |
---|---|
EP (1) | EP0196921A3 (ko) |
JP (1) | JPS62282A (ko) |
KR (1) | KR860008282A (ko) |
AU (1) | AU5559786A (ko) |
DK (1) | DK152886A (ko) |
ES (1) | ES8800723A1 (ko) |
FI (1) | FI861428A (ko) |
GR (1) | GR860847B (ko) |
HU (1) | HUT40698A (ko) |
IL (1) | IL78314A0 (ko) |
NO (1) | NO861302L (ko) |
PT (1) | PT82311B (ko) |
ZA (1) | ZA862479B (ko) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0161866B1 (en) * | 1984-05-03 | 1992-07-01 | Kazuo Murakami | Human renin genomic dna |
DE3438545A1 (de) * | 1984-10-20 | 1986-04-24 | Merck Patent Gmbh, 6100 Darmstadt | Peptide |
JPS63502397A (ja) * | 1986-02-20 | 1988-09-14 | カロビオ エービー | ステロイドレセプタ−タンパクの真核細胞における発現 |
GB2200118A (en) * | 1987-01-23 | 1988-07-27 | Allelix Inc | Synthetic chymosin-and prochymosin-encoding DNA segments |
CA2020720A1 (en) * | 1989-07-13 | 1991-01-14 | Shigeko Yamazaki | Method for the purification of therapeutically active recombinant acidic fibroblast growth factor |
DE4136443A1 (de) * | 1991-11-06 | 1993-05-13 | Boehringer Ingelheim Int | Expression der reifen proteinase 2a, ihre partielle reinigung und bereitstellung kompetitiv wirkender substrate |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1983001783A1 (en) * | 1981-11-23 | 1983-05-26 | University Patents Inc | Control of dna sequence transcription |
JPH0683670B2 (ja) * | 1983-08-12 | 1994-10-26 | 第一製薬株式会社 | デオキシリボ核酸 |
EP0161866B1 (en) * | 1984-05-03 | 1992-07-01 | Kazuo Murakami | Human renin genomic dna |
WO1986004920A1 (en) * | 1985-02-13 | 1986-08-28 | Biotechnology Research Partners, Limited | Human metallothionein-ii promoter in mammalian expression system |
-
1986
- 1986-03-28 IL IL78314A patent/IL78314A0/xx unknown
- 1986-03-31 GR GR860847A patent/GR860847B/el unknown
- 1986-04-01 PT PT82311A patent/PT82311B/pt unknown
- 1986-04-02 NO NO861302A patent/NO861302L/no unknown
- 1986-04-02 FI FI861428A patent/FI861428A/fi not_active IP Right Cessation
- 1986-04-02 ES ES553644A patent/ES8800723A1/es not_active Expired
- 1986-04-02 EP EP86302432A patent/EP0196921A3/en not_active Withdrawn
- 1986-04-02 AU AU55597/86A patent/AU5559786A/en not_active Abandoned
- 1986-04-02 HU HU861355A patent/HUT40698A/hu unknown
- 1986-04-03 JP JP61077502A patent/JPS62282A/ja active Pending
- 1986-04-03 ZA ZA862479A patent/ZA862479B/xx unknown
- 1986-04-03 KR KR1019860002529A patent/KR860008282A/ko not_active Application Discontinuation
- 1986-04-03 DK DK152886A patent/DK152886A/da not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
IL78314A0 (en) | 1986-07-31 |
HUT40698A (en) | 1987-01-28 |
DK152886A (da) | 1986-10-04 |
PT82311A (en) | 1986-05-01 |
ES8800723A1 (es) | 1987-11-16 |
DK152886D0 (da) | 1986-04-03 |
FI861428A0 (fi) | 1986-04-02 |
ES553644A0 (es) | 1987-11-16 |
PT82311B (en) | 1987-09-08 |
EP0196921A2 (en) | 1986-10-08 |
JPS62282A (ja) | 1987-01-06 |
GR860847B (en) | 1986-07-31 |
EP0196921A3 (en) | 1987-12-02 |
FI861428A (fi) | 1986-10-04 |
NO861302L (no) | 1986-10-06 |
AU5559786A (en) | 1986-10-09 |
ZA862479B (en) | 1986-12-30 |
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