KR900700618A - 인터루킨-1억제제 - Google Patents

인터루킨-1억제제

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KR900700618A
KR900700618A KR1019890702466A KR890702466A KR900700618A KR 900700618 A KR900700618 A KR 900700618A KR 1019890702466 A KR1019890702466 A KR 1019890702466A KR 890702466 A KR890702466 A KR 890702466A KR 900700618 A KR900700618 A KR 900700618A
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interleukin
inhibitor
dna
sequence
dna sequence
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KR1019890702466A
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KR0148009B1 (ko
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에이취. 하논 챨스
피. 아이젠버그 스티븐
씨. 톰슨 로버트
피. 아렌드 윌리암
지. 조슬린 페니크
소머 안드레아스
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원본미기재
씨네겐 인코포레이티드
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Priority to KR1019960703435A priority Critical patent/KR970002917B1/ko
Priority claimed from PCT/US1989/002275 external-priority patent/WO1989011540A1/en
Publication of KR900700618A publication Critical patent/KR900700618A/ko
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Publication of KR0148009B1 publication Critical patent/KR0148009B1/ko

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Abstract

내용 없음

Description

인터루킨-1억제제
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1a도와 제1b도는 2개의 대사적으로-표지시킨 단구 부유물을 Mono Q 크로마토그라피하여 단백질 프로필을 도시한 것이다. 이 세포들은 IgC(제1a도) 또는 태아송아지혈청(제1b도)이 도포된 플레이트에서 배양시킨 것이다.
제2a도는 제1b도에서 지적된 부분의 각 분획들을 전개시킨 겔을 은-염색하여 나타낸 것이다.
제2b도는 제2a도에서 나타낸 겔의 방사선사진이다.
제3a도 및 제3b도 및 제3c도는 실시예 1에서 얻어진 정제된 IL-li에 대한 자료로서, 제3a도는 2중 방사선 패턴을 갖는 크로마 토그라피 자료이고, 제3b도는 제3a도에서 지적된 분획의 시료를 전개시킨 겔을 은-염색하여 나타낸 것이며, 제3c도는 제3b도에 나타낸 겔의 방사선 사진이다.

Claims (25)

  1. 인터루킨-1α 및 인터루킨-1β 중에서 선택된 적어도 하나 이상의 물질에 대해 활성을 갖는 실질적으로 정제된 인터루킨-1 억제제.
  2. 제1항에 있어서의 인터루킨-1 억제제는 포유동물세포에서 유도된 것임을 특징으로 하는 인터루킨-1억제제.
  3. 제1항에 있어서의 인터루킨-1 억제제는 단구로부터 분리된 것임을 특징으로 하는 인터루킨-1억제제.
  4. 제3항에 있어서의 인터루킨-1 억제제는 인간단구로부터 분리된 것임을 특징으로 하는 인터루킨-1억제제.
  5. 제1항에 있어서의 인터루킨-1 억제제는 재조합-DNA 방법에 의해 생산된 것임을 특징으로 하는 인터루킨-1 억제제.
  6. 인터루킨-1 억제제를 제조하는데 있어서, (가) 인터루킨-1 억제 활성을 갖는 단백질을 생산하도록 숙주세포를 조종할 수 있는 DNA시퀀스를 준비한 후, (나) 상기 DNA 시퀀스를 그 DNA시퀀스를 발현시키는데 필요한 작동요소가 포함되어있고 숙주세포로 전이되어 복제될 수 있는 벡터에 클로닝시키고, (다) 상기 합성 DNA시퀀스와 작동요소가 포함되어 있는 벡터를 인터루킨-1 억제제를 코드화하고 있는 DNA를 발현시킬 수 있는 숙주세포로 전이시킨 다음, (라) 상기 숙주세포를 벡터를 증폭시키고 억제제를 발현시키기에 적당한 조건하에서 배양하고, (마) 상기 억제제를 수확하여, (바) 상기 억제제가 활성적인 3차구를 취하도록 하며 인터루킨-1 억제활성을 갖도록 한 것임을 특징으로 하는 DNA-재조합 방법에 의한 인터루킨-1 억제제의 제조방법.
  7. 제7항에 있어서, 상기 DNA 시퀀스는 상보 DNA인 것임을 특징으로 하는 인터루킨-1 억제제의 제조방법.
  8. 제6항에 있어서, 상기 DNA 시퀀스는 제놈 폴리뉴클레오티드 시퀀스인 것임을 특징으로 하는 인터루킨-1 억제제의 제조방법.
  9. 제6항에 있어서, 상기 DNA 시퀀스는 포유동물등을 세포로부터 유도된 것임을 특징으로 하는 인터루킨-1 억제제의 제조방법.
  10. 제9항에 있어서, 상기 DNA 시퀀스는 인간 단구 세포로부터 유도된 것임을 특징으로 하는 인터루킨-1 억제제의 제조방법.
  11. 제6항에 있어서, 상기 숙주 세포는 미생물인 것임을 특징으로 하는 인터루킨-1 억제제의 제조방법.
  12. 제11항에 있어서, 상기 미생물은 E. coli인 것임을 특징으로 하는 인터루킨-1 억제제의 제조방법.
  13. 제6항에 있어서, 상기 숙주세포는 포유동물 세포인 것임을 특징으로 하는 인터루킨-1 억제제의 제조방법.
  14. 제13항에 있어서, 상기 포유동물 세포는 CHO세포인 것임을 특징으로 하는 인터루킨-1 억제제의 제조방법.
  15. 제6항에 있어서, 상기 DNA 시퀀스는 상보 DNA 뉴클레오티드 시퀀스인 것임을 특징으로 하는 인터루킨-1 억제제의 제조방법.
  16. 제6항에 있어서, 상기 DNA 시퀀스는 제놈 뉴클레오티드 시퀀스인 것임을 특징으로 하는 인터루킨-1 억제제의 제조방법.
  17. 인터루킨-1 억제제와 충분히 반복적인 아미노산 시퀀스를 함유하는 적어도 하나이상의 인터루킨-1 억제제의 활성을 갖도록 되어진 폴리펩타이드를 코드화하고 있는 DNA 시퀀스로 이루어진 것임을 특징으로 하는 생리학적 기능을 갖는 인터루킨-1 억제제를 코드화 하고 있는 분리된 DNA 시퀀스.
  18. 제17항에 있어서의 분리된 DNA 시퀀스는 그 DNA 염기 시퀀스가 다음 시퀀스의 99위치와 557위치 사이의 핵산들을 함유하는 것임을 특징으로 하는 분리된 DNA 시퀀스.
  19. 재조합. DNA 분자 GT10-ILIi-2A(ATCC 49488).
  20. 인터루킨-1 억제제 X와 인터루킨-1 억제제 α 및 인터루킨-1β 중 적어도 하나 이상으로 이루어진 것임을 특징으로 하는 실질적으로 정제된 인터루킨-1 억제제.
  21. 제20항에 있어서의 인터루킨-1 억제제는 인터루킨-1 억제제 X인 것임을 특징으로 하는 인터루킨-1 억제제.
  22. 제20항에 있어서의 인터루킨-1 억제제는 인터루킨-1 억제제 α 인 것임을 특징으로 하는 인터루킨-1 억제제.
  23. 제20항에 있어서의 인터루킨-1 억제제는 인터루킨-1 억제제 β인 것임을 특징으로 하는 인터루킨-1 억제제.
  24. 제17항에 있어서의 분리된 DNA 시퀀스는 그 DNA 염기 시퀀스.
  25. 다음의 시퀀스를 갖는 실질적으로 정제된 인터루킨-1 억제제.
    여기서, X는 시스테인, 세린 또는 알라닌이며, Z는 아르기닌 또는 프톨린이다.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019890702466A 1988-05-27 1989-05-25 인터루킨-1 억제제 KR0148009B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019960703435A KR970002917B1 (ko) 1988-05-27 1989-05-25 인터루킨-i 억제제

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US238,713 1981-02-27
US19991588A 1988-05-27 1988-05-27
US199,915 1988-05-27
US23871388A 1988-08-31 1988-08-31
US24852188A 1988-09-23 1988-09-23
US248,521 1988-09-23
US26653188A 1988-11-03 1988-11-03
US266,531 1988-11-03
PCT/US1989/002275 WO1989011540A1 (en) 1988-05-27 1989-05-25 Interleukin-1 inhibitors

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AT (2) ATE88502T1 (ko)
CA (1) CA1341322C (ko)
DE (4) DE68906077T2 (ko)
ES (2) ES2018750T3 (ko)
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US5837495A (en) * 1994-10-13 1998-11-17 Applied Research Systems Ars Holding N.V. DNA encoding interleukin-1 antagonist
US5981713A (en) * 1994-10-13 1999-11-09 Applied Research Systems Ars Holding N.V. Antibodies to intereleukin-1 antagonists
IT1270662B (it) * 1994-10-13 1997-05-07 Applied Research Systems Antagonista della interleuchina-1
HUP9900169A2 (hu) * 1995-09-13 2000-03-28 Takeda Chemical Industries, Ltd. Immunoszuppresszáns hatású kinolin- vagy kinozalinszármazékok és ezeket tartalmazó gyógyászati készítmények
TW555765B (en) 1996-07-09 2003-10-01 Amgen Inc Low molecular weight soluble tumor necrosis factor type-I and type-II proteins
US6294170B1 (en) 1997-08-08 2001-09-25 Amgen Inc. Composition and method for treating inflammatory diseases
US7205381B2 (en) 1997-04-21 2007-04-17 Schering Corporation Mammalian cytokines; related reagents and methods
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JP2002510492A (ja) 1998-04-03 2002-04-09 ハイセック，インコーポレーテッド インターロイキン−１レセプターアンタゴニストおよびその使用
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US6680380B1 (en) 1998-09-18 2004-01-20 Schering Corporation Nucleic acids encoding mammalian interleukin-1ζ, related reagents and methods
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Also Published As

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ES2018750A4 (es) 1991-05-16
DE68906077T2 (de) 1993-07-29
ATE215966T1 (de) 2002-04-15
EP0343684B1 (en) 1993-04-21
EP1133995A2 (en) 2001-09-19
EP0541920B1 (en) 2002-04-10
DE68929387T2 (de) 2002-08-14
DE10299011I1 (de) 2002-07-18
EP1133995A3 (en) 2001-09-26
HK1017821A1 (en) 1999-11-26
ATE88502T1 (de) 1993-05-15
CA1341322C (en) 2001-11-27
EP0343684A1 (en) 1989-11-29
IE950317L (en) 1989-11-27
DE68929387D1 (de) 2002-05-16
NL300091I1 (nl) 2002-07-01
DE343684T1 (de) 1990-11-08
NZ229326A (en) 1992-12-23
IE66712B1 (en) 1996-01-24
HK1039901A1 (zh) 2002-05-17
DE68906077D1 (de) 1993-05-27
NL300091I2 (nl) 2002-08-01
KR0148009B1 (ko) 1998-08-01
ES2018750T3 (es) 1994-10-16
ES2176181T3 (es) 2002-12-01
EP0541920A1 (en) 1993-05-19
DE10299011I2 (de) 2006-03-16
IE891781L (en) 1989-11-27
IL90423A0 (en) 1990-01-18
LU90943I2 (fr) 2002-09-30
SG59969A1 (en) 1999-02-22
GR900300115T1 (en) 1991-09-27
IL90423A (en) 2000-07-26

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