KR840004781A - 인체의 조직 플라스미노겐 활성화제 - Google Patents

인체의 조직 플라스미노겐 활성화제 Download PDF

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KR840004781A
KR840004781A KR1019830001902A KR830001902A KR840004781A KR 840004781 A KR840004781 A KR 840004781A KR 1019830001902 A KR1019830001902 A KR 1019830001902A KR 830001902 A KR830001902 A KR 830001902A KR 840004781 A KR840004781 A KR 840004781A
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tissue plasminogen
plasminogen activator
human body
human
host cell
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KR1019830001902A
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반노만 괴델 데이비드 (외 3)
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월터 에이치·드레거
제넨테크 인코오포레이티드
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Application filed by 월터 에이치·드레거, 제넨테크 인코오포레이티드 filed Critical 월터 에이치·드레거
Publication of KR840004781A publication Critical patent/KR840004781A/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • Wood Science & Technology (AREA)
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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
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  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)

Abstract

내용 없음

Description

인체의 조직 플라스미노겐 활성화제
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 프로테아제 방해제를 갖고 있는 흑종세포 및 이것을 갖고 있지 않는 흑종세포로부터 추출된 항t-PA IgA와 함께 침전 가능한 35S 라벨을 붙인 단백질의 10% SDS 파지(PAGE)이다.
제6도는 표현 플라스미드 PΔPIPA˚의 조립 방식을 도식한 것이다.
제12도는 본 명세서의 실시예 1에 예시한 방법에 의해서 제조된 것으로서 인체의 조식 플라스미노겐 활성화제의 모형도이다.

Claims (15)

  1. 인체에 출처를 둔 다른 단백질은 실질적으로 함유하지 않는 인체의 조직 플라스미노겐 활성화제.
  2. 관련 천연 글리코실화 반응을 수반하지 않는 인체의 조직 플라스미노겐 활성화제.
  3. 조환형 숙주 세포에 의해서 제조된 인체의 조직 플라스미노겐.
  4. 실질적으로 순수한 형태로 있는 인체의 조직 플라스미노겐 활성화제.
  5. 위의 인체의 조직 플라스미노겐 활성화제의 통상의 제1아미노산의 N말단에서부터 계속되는 폴리에티드의 결합 서열을 함유하는 제1―4항에 따르는 인체의 조직 플라스미노겐 활성화제.
  6. 인체의 조직 플라스미노겐 활성화제의 결합서열로 구성된 DNA 결합 서열.
  7. 제6항에 따르는 DNA 결합서열을 변성체 미생물 또는 세포 배지에서 표현할 수 있는 복제 가능한 표현 벡터.
  8. 플라스미드 PΔPIPA˚또는 pt-PAtrrp12.
  9. 제7항 또는 제8항에 따르는 담체로 변성시킨 미생물 또는 세포 배지.
  10. 제9항에 따르는 E. coli종을 변성시킴으로써 얻은 미생물체.
  11. 약리학적으로 허용되는 담체와의 혼합물의 상태로 있는 제1―5항에 따르는 인체의 조직 플라스미노겐 활성화제의 약리학적으로 유효한 양으로 구성된 조성물.
  12. 제11항에 따르는 비경구 투여용으로 적합한 조성물.
  13. 맥관 질병의 치료를 위한 또는 이와같은 치료에 사용되는 약리학적 조성물을 제조하기 위한 제1―5항에 따르는 인체의 조직 플라스미노겐 활성화제.
  14. 조환형 숙주 세포 내에서 인체의 조직 플라스미노겐 활성화제를 암호화하는 DNA를 표현하는 것으로 구성되는 방법.
  15. 적절한 숙주 세포내에서 인체의 t-PA를 암호화하는 DNA 결합 서열을 표현할 수 있는 복제 가능한 표현매체를 제조하는 제1단계, 숙주 세포 배지를 변성시켜 조환형 숙주세포를 얻는 제2단계, DNA 결합서열을 암호화하는 위의 t-PA를 표현함으로써 인체의 t-PA를 제조할 수 있는 조건하에서 위의 조환형 숙주 세포를 배양하는 제3단계, 그리고 위의 인체의 t-PA를 회수하는 제4단계로 구성됨을 특징으로 하는 인체의 t-PA의 제조방법.
    ※참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019830001902A 1982-05-05 1983-05-04 인체의 조직 플라스미노겐 활성화제 KR840004781A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37486082A 1982-05-05 1982-05-05
US374860 1982-05-05

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Country Status (8)

Country Link
US (1) US5763253A (ko)
JP (2) JPS5942321A (ko)
KR (1) KR840004781A (ko)
GT (1) GT198302091A (ko)
HU (1) HU200615B (ko)
MX (1) MX197183A (ko)
SU (1) SU1662352A3 (ko)
ZA (1) ZA833174B (ko)

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AU554862B2 (en) * 1982-12-14 1986-09-04 Implico B.V. Plasminogen activator isolated by affinity chromatography
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DE3588149T2 (de) * 1984-10-01 1997-10-09 Genzyme Corp Rekombinante DNS-Verfahren und dadurch hergestellte Produkte
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Publication number Publication date
JPH0134596B2 (ko) 1989-07-20
ZA833174B (en) 1984-08-29
GT198302091A (es) 1984-10-25
JPS5942321A (ja) 1984-03-08
US5763253A (en) 1998-06-09
JPS6291187A (ja) 1987-04-25
HU200615B (en) 1990-07-28
JPS6216931B2 (ko) 1987-04-15
MX197183A (es) 1994-02-28
SU1662352A3 (ru) 1991-07-07

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