ES8605296A1 - Procedimiento de producir factor viii-r humano - Google Patents

Procedimiento de producir factor viii-r humano

Info

Publication number
ES8605296A1
ES8605296A1 ES546110A ES546110A ES8605296A1 ES 8605296 A1 ES8605296 A1 ES 8605296A1 ES 546110 A ES546110 A ES 546110A ES 546110 A ES546110 A ES 546110A ES 8605296 A1 ES8605296 A1 ES 8605296A1
Authority
ES
Spain
Prior art keywords
factor viii
protein
self
recominant
human
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES546110A
Other languages
English (en)
Other versions
ES546110A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Meloy Laboratories Inc
Original Assignee
Meloy Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Meloy Laboratories Inc filed Critical Meloy Laboratories Inc
Publication of ES8605296A1 publication Critical patent/ES8605296A1/es
Publication of ES546110A0 publication Critical patent/ES546110A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Abstract

PROCEIDMIENTO DE PRODUCTOR FACTOR VIII-R HUMANO. COMPRENDE: A) PREPARAR UN DS-CDNA A PARTIR DE UNA POBLACION DE RNA MENSAJERO QUE CONTIENE RNA MENSAJERO Y CODIFICA AL FACTOR VIII-R; B) INCORPORAR EL DNA DEL DS-CDNA, EN UN VECTOR DE EXPRESION REPLICABLE, PARA OBTENER UN VECTOR DE EXPRESION REPLICABLE QUE EXPRESA LA SECUENCIA DNA QUE CODIFICA AL FACTOR VIII-R HUMANO EN UN SISTEMA HUESPED RECOMBINANTE; C) TRANSFORMAR AL SISTEMA HUESPED EN UN SISTEMA RECOMBINANTE; D) MANTENER AL SISTEMA HUESPED RECOMBINANTE EN CONDICIONES ADECUADAS, PARA PRODUCIR FACTOR VIII-R; E) HACER REACCIONAR A LAS PROTEINAS EXPRESADAS POR EL SISTEMA HUESPED RECOMBINANTE CON UNA COMPOSICION REACTIVA; Y F) OBSERVAR TODA RESPUESTA DETECTABLE DE LA REACCION DE E) Y AISLAR EL FACTOR VIII-R DETECTADO DEL SISTEMA HUESPED. EL VECTOR DE EXPRESION ES UN BACTERIOFAGO LAMBDA GT10 O LAMBDA GT11 Y LA COMPOSICION REACTIVA ESTA FORMADA POR ANTICUERPO PRIMARIO ESPECIFICO PARA VIII-R Y UN ANTICUERPO SECUNDARIO ASOCIADO A UNA SUSTANCIA DE RESPUESTA DETECTABLE QUE TIENE UN MARCADOR ISOTOPICO O NO, UNA ENZIMA, UN SUSTRATO Y UN REACTIVO DE INTERACCION DE LA ENZIMA.
ES546110A 1984-07-27 1985-07-26 Procedimiento de producir factor viii-r humano Expired ES8605296A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US63493384A 1984-07-27 1984-07-27

Publications (2)

Publication Number Publication Date
ES8605296A1 true ES8605296A1 (es) 1986-04-01
ES546110A0 ES546110A0 (es) 1986-04-01

Family

ID=24545739

Family Applications (1)

Application Number Title Priority Date Filing Date
ES546110A Expired ES8605296A1 (es) 1984-07-27 1985-07-26 Procedimiento de producir factor viii-r humano

Country Status (10)

Country Link
EP (1) EP0169562A1 (es)
JP (1) JPS6143121A (es)
KR (1) KR860001189A (es)
AU (1) AU4538285A (es)
DK (1) DK341485A (es)
ES (1) ES8605296A1 (es)
FI (1) FI852866L (es)
IL (1) IL75919A0 (es)
NO (1) NO852974L (es)
ZA (1) ZA855648B (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965199A (en) * 1984-04-20 1990-10-23 Genentech, Inc. Preparation of functional human factor VIII in mammalian cells using methotrexate based selection
DK525384D0 (da) * 1984-11-05 1984-11-05 Nordisk Insulinlab Praeparat paa basis af faktor viii til behandling af haemofili a inhibitorpatienter samt fremgangsmaade til fremstilling af et saadan praeparat
AU591671B2 (en) * 1985-04-11 1989-12-14 Children's Medical Center Corporation Von willebrand factor
US8597910B1 (en) 1985-04-11 2013-12-03 Children's Medical Center Corporation DNA encoding Von Willebrand Factor (VWF) and methods and cells for producing VFW, and VFW produced by the DNA, methods and cells
JPS63500144A (ja) * 1985-05-16 1988-01-21 メロイ　ラボラトリ−ズ　インコ−ポレ−テツド ヒト因子８Ｒ（フオンウイルブランド因子）にｃＤＮＡを分子クロ−ン化する方法
US5198349A (en) * 1986-01-03 1993-03-30 Genetics Institute, Inc. Method for producing factor VIII:C and analogs
US5849536A (en) * 1990-03-02 1998-12-15 Bio-Technology General Corp. Cloning and production of human von willebrand factor GPIb binding domain polypeptides and methods of using same
US6008193A (en) * 1990-03-02 1999-12-28 Bio-Technology General Corp. Methods of using human von Willebrand factor GPIb binding domain polypeptides

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4361509A (en) * 1981-12-14 1982-11-30 Scripps Clinic And Research Foundation Ultrapurification of factor VIII using monoclonal antibodies

Also Published As

Publication number Publication date
NO852974L (no) 1986-01-28
FI852866L (fi) 1986-01-28
DK341485D0 (da) 1985-07-26
ZA855648B (en) 1986-03-26
EP0169562A1 (en) 1986-01-29
DK341485A (da) 1986-01-28
FI852866A0 (fi) 1985-07-23
KR860001189A (ko) 1986-02-24
AU4538285A (en) 1986-01-30
JPS6143121A (ja) 1986-03-01
ES546110A0 (es) 1986-04-01
IL75919A0 (en) 1985-12-31

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