ES8800723A1 - Un metodo para preparar renina humana. - Google Patents

Un metodo para preparar renina humana.

Info

Publication number
ES8800723A1
ES8800723A1 ES553644A ES553644A ES8800723A1 ES 8800723 A1 ES8800723 A1 ES 8800723A1 ES 553644 A ES553644 A ES 553644A ES 553644 A ES553644 A ES 553644A ES 8800723 A1 ES8800723 A1 ES 8800723A1
Authority
ES
Spain
Prior art keywords
renin
recombinant human
prorenin
disclosed
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES553644A
Other languages
English (en)
Other versions
ES553644A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BIOTECHNOLOGY RES ASS
Original Assignee
BIOTECHNOLOGY RES ASS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BIOTECHNOLOGY RES ASS filed Critical BIOTECHNOLOGY RES ASS
Publication of ES553644A0 publication Critical patent/ES553644A0/es
Publication of ES8800723A1 publication Critical patent/ES8800723A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6486—Renin (3.4.23.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23015—Renin (3.4.23.15)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

METODO PARA PRODUCIR RENINA HUMANA. COMPRENDE: A) TRANSFECTAR LAS CELULAS CON UN VECTOR QUE HA SIDO TRATADO POR INGENIERIA GENETICA PARA CONTENER UN ADN QUE TIENE LA SECUENCIA CODIFICADORA DE RENINA; B) CULTIVAR LAS CELULAS TRANSFECTADAS EN UN MEDIO DE CULTIVO QUE CONTIENEN LOS INGREDIENTES REQUERIDOS A UN PH ENTRE 6 Y 8 Y ENTRE 32J Y 39JC, PARA HACER CRECER A LAS CELULAS; C) INDUCIR EN EL CULTIVO LA UNION DE LAS SECUENCIAS DE CONTROL CON LA SECUENCIA CODIFICADORA DE RENINA; D) RECUPERAR LA RENINA DEL CULTIVO AL CONVERTIR PRORRENINA EN RENINA Y PURIFICAR LA RENINA OBTENIDA MEDIANTE CROMATOGRAFIA DE INTERACCION HIDROFOBA Y CROMATOGRAFIA DE INTERCAMBIO IONICO. EL CULTIVO CELULAR ES DE CELULAS DE MAMIFERO QUE TIENE UNA CONCENTRACION EFICAZ DE ION DE METAL PESADO DE 1-5 D 10C5 M DE ZNB2. SE UTILIZAN COMO VASOCONTRICTOR/DILATADOR PARA TRATAR LA HIPERTENSION.
ES553644A 1985-04-03 1986-04-02 Un metodo para preparar renina humana. Expired ES8800723A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US71941485A 1985-04-03 1985-04-03

Publications (2)

Publication Number Publication Date
ES553644A0 ES553644A0 (es) 1987-11-16
ES8800723A1 true ES8800723A1 (es) 1987-11-16

Family

ID=24889982

Family Applications (1)

Application Number Title Priority Date Filing Date
ES553644A Expired ES8800723A1 (es) 1985-04-03 1986-04-02 Un metodo para preparar renina humana.

Country Status (13)

Country Link
EP (1) EP0196921A3 (es)
JP (1) JPS62282A (es)
KR (1) KR860008282A (es)
AU (1) AU5559786A (es)
DK (1) DK152886A (es)
ES (1) ES8800723A1 (es)
FI (1) FI861428A (es)
GR (1) GR860847B (es)
HU (1) HUT40698A (es)
IL (1) IL78314A0 (es)
NO (1) NO861302L (es)
PT (1) PT82311B (es)
ZA (1) ZA862479B (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3586266T2 (de) * 1984-05-03 1993-02-18 Kazuo Murakami Genomische dns menschlichen renins.
DE3438545A1 (de) * 1984-10-20 1986-04-24 Merck Patent Gmbh, 6100 Darmstadt Peptide
WO1987005049A1 (en) * 1986-02-20 1987-08-27 California Biotechnology Inc. Eucaryotic expression of steroid receptor proteins
GB2200118A (en) * 1987-01-23 1988-07-27 Allelix Inc Synthetic chymosin-and prochymosin-encoding DNA segments
CA2020720A1 (en) * 1989-07-13 1991-01-14 Shigeko Yamazaki Method for the purification of therapeutically active recombinant acidic fibroblast growth factor
DE4136443A1 (de) * 1991-11-06 1993-05-13 Boehringer Ingelheim Int Expression der reifen proteinase 2a, ihre partielle reinigung und bereitstellung kompetitiv wirkender substrate

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0094428B1 (en) * 1981-11-23 1992-02-12 University Patents, Inc. Control of dna sequence transcription
JPH0683670B2 (ja) * 1983-08-12 1994-10-26 第一製薬株式会社 デオキシリボ核酸
DE3586266T2 (de) * 1984-05-03 1993-02-18 Kazuo Murakami Genomische dns menschlichen renins.
ATE89314T1 (de) * 1985-02-13 1993-05-15 Scios Nova Inc Menschlicher metallothionein ii-promotor in saeugetierexpressionssystemen.

Also Published As

Publication number Publication date
ES553644A0 (es) 1987-11-16
EP0196921A2 (en) 1986-10-08
PT82311B (en) 1987-09-08
FI861428A (fi) 1986-10-04
AU5559786A (en) 1986-10-09
DK152886A (da) 1986-10-04
IL78314A0 (en) 1986-07-31
PT82311A (en) 1986-05-01
JPS62282A (ja) 1987-01-06
DK152886D0 (da) 1986-04-03
NO861302L (no) 1986-10-06
GR860847B (en) 1986-07-31
HUT40698A (en) 1987-01-28
ZA862479B (en) 1986-12-30
FI861428A0 (fi) 1986-04-02
KR860008282A (ko) 1986-11-14
EP0196921A3 (en) 1987-12-02

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