AR012499A1 - Una secuencia quimerica de acido nucleico que codifica una proteina de fusion, un vector de expresion que comprende dicha secuencia quimerica, una celula huesped transformada que contiene dicho vector, la proteina de fusion obtenida y un metodo in vitro para la preparacion de un polipeptido recombin - Google Patents

Una secuencia quimerica de acido nucleico que codifica una proteina de fusion, un vector de expresion que comprende dicha secuencia quimerica, una celula huesped transformada que contiene dicho vector, la proteina de fusion obtenida y un metodo in vitro para la preparacion de un polipeptido recombin

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Publication number
AR012499A1
AR012499A1 ARP980101873A ARP980101873A AR012499A1 AR 012499 A1 AR012499 A1 AR 012499A1 AR P980101873 A ARP980101873 A AR P980101873A AR P980101873 A ARP980101873 A AR P980101873A AR 012499 A1 AR012499 A1 AR 012499A1
Authority
AR
Argentina
Prior art keywords
fusion protein
vector
chemical sequence
repared
vito
Prior art date
Application number
ARP980101873A
Other languages
English (en)
Original Assignee
Sembiosys Genetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sembiosys Genetics Inc filed Critical Sembiosys Genetics Inc
Publication of AR012499A1 publication Critical patent/AR012499A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/02—Nutrients, e.g. vitamins, minerals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormones [GH] (Somatotropin)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Abstract

Se describe un método mejorado para recuperar polipéptidos producidos de manera recombinante. El método incluye expresar el polipéptido recombinantecomo una proteína de fusion con un pro-péptido. La proteína de fusion pro-péptido-polipép tido puede clivarse y el polipéptido recombinante puede serliberado bajo condiciones apropiadas
ARP980101873A 1997-04-25 1998-04-23 Una secuencia quimerica de acido nucleico que codifica una proteina de fusion, un vector de expresion que comprende dicha secuencia quimerica, una celula huesped transformada que contiene dicho vector, la proteina de fusion obtenida y un metodo in vitro para la preparacion de un polipeptido recombin AR012499A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US4425497P 1997-04-25 1997-04-25

Publications (1)

Publication Number Publication Date
AR012499A1 true AR012499A1 (es) 2000-10-18

Family

ID=21931348

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP980101873A AR012499A1 (es) 1997-04-25 1998-04-23 Una secuencia quimerica de acido nucleico que codifica una proteina de fusion, un vector de expresion que comprende dicha secuencia quimerica, una celula huesped transformada que contiene dicho vector, la proteina de fusion obtenida y un metodo in vitro para la preparacion de un polipeptido recombin

Country Status (18)

Country Link
US (1) US7501265B1 (es)
EP (1) EP0977873B1 (es)
JP (1) JP4120972B2 (es)
KR (2) KR100635623B1 (es)
AR (1) AR012499A1 (es)
AT (1) ATE341633T1 (es)
AU (1) AU742794B2 (es)
BR (1) BR9809416A (es)
CA (1) CA2286861A1 (es)
DE (1) DE69836075T2 (es)
DK (1) DK0977873T3 (es)
ES (1) ES2273414T3 (es)
IL (2) IL132259A0 (es)
MX (1) MX219629B (es)
NZ (1) NZ500736A (es)
TW (1) TW505697B (es)
WO (1) WO1998049326A1 (es)
ZA (1) ZA983471B (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003531567A (ja) * 1999-04-30 2003-10-28 オーソ−マクニール・フアーマシユーチカル・インコーポレーテツド チモーゲン活性化系
US6420157B1 (en) * 1999-04-30 2002-07-16 Ortho-Mcneil Pharmaceutical, Inc. Zymogen activation system
TR200200048T2 (tr) * 1999-08-09 2002-04-22 Biochemie Gesellschaft M.B.H. Otoproteolitik klevaj vasıtasıyla proteinlerin üretimi.
TR200200253T2 (tr) * 1999-08-09 2002-05-21 Bio-Chemie Gesellschaft M.B.H. Proteinlerin üretimi
CA2381438C (en) * 1999-08-23 2010-10-05 Sembiosys Genetics Inc. Commercial production of chymosin in plants
US7390936B1 (en) 1999-08-23 2008-06-24 Sembiosys Genetics Inc. Commercial production of chymosin in plants
US6750034B1 (en) 2000-06-30 2004-06-15 Ortho-Mcneil Pharmaceutical, Inc. DNA encoding human serine protease D-G
US6737262B1 (en) 2000-07-11 2004-05-18 Robert I. Bolla Animal feed containing polypeptides
AU2004247762B2 (en) 2003-06-17 2010-05-27 Sembiosys Genetics Inc. Methods for the production of insulin in plants
CN101454452B (zh) * 2003-08-06 2014-01-01 马里兰大学生物技术研究所 用于亲和纯化的工程蛋白酶及融合蛋白的加工
CN102389575B (zh) * 2011-11-18 2013-01-23 武汉凯肽来生物科技有限公司 一种基因工程口服dna疫苗及制备方法和应用
WO2013142859A2 (en) * 2012-03-23 2013-09-26 Wuhan Peptech Pharmaceutical Co., Ltd. Fusion proteins of superfolder green fluorescent protein and use thereof
IL301396A (en) 2020-09-30 2023-05-01 Nobell Foods Inc Recombinant milk proteins and food compositions containing them
US10894812B1 (en) 2020-09-30 2021-01-19 Alpine Roads, Inc. Recombinant milk proteins
US10947552B1 (en) 2020-09-30 2021-03-16 Alpine Roads, Inc. Recombinant fusion proteins for producing milk proteins in plants

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4180559A (en) * 1978-01-05 1979-12-25 Richardson-Merrell Inc. Coated 1-(2-chlorodibenzo[b,f]oxepin-10-yl)-4-methylpiperazine compositions
US4774183A (en) * 1983-06-29 1988-09-27 Kansas State University Research Foundation Process for producing fructose
AU3036684A (en) * 1983-07-07 1985-01-10 Genex Corp. Production of bovine calf chymosin
US4743679A (en) 1986-02-24 1988-05-10 Creative Biomolecules, Inc. Process for producing human epidermal growth factor and analogs thereof
DE69101486T2 (de) 1990-01-24 1994-08-04 Upjohn Co Verfahren zur reinigung rekombinanter polypeptide.
US5650554A (en) * 1991-02-22 1997-07-22 Sembiosys Genetics Inc. Oil-body proteins as carriers of high-value peptides in plants
CA2153254C (en) * 1993-01-15 2002-05-21 Edward R. Lavallie Cloning of enterokinase and method of use
US6265204B1 (en) * 1997-01-17 2001-07-24 Genencor International, Inc. DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi

Also Published As

Publication number Publication date
MX9909801A (es) 2000-03-31
DE69836075D1 (de) 2006-11-16
CA2286861A1 (en) 1998-11-05
DK0977873T3 (da) 2007-02-05
US7501265B1 (en) 2009-03-10
AU7024098A (en) 1998-11-24
DE69836075T2 (de) 2007-06-06
KR20010006506A (ko) 2001-01-26
EP0977873B1 (en) 2006-10-04
ATE341633T1 (de) 2006-10-15
ZA983471B (en) 1998-10-27
EP0977873A1 (en) 2000-02-09
KR100635623B1 (ko) 2006-10-18
KR100609886B1 (ko) 2006-08-18
NZ500736A (en) 2005-01-28
TW505697B (en) 2002-10-11
JP4120972B2 (ja) 2008-07-16
WO1998049326A1 (en) 1998-11-05
KR20050100712A (ko) 2005-10-19
JP2001527400A (ja) 2001-12-25
BR9809416A (pt) 2000-06-13
IL132259A0 (en) 2001-03-19
AU742794B2 (en) 2002-01-10
MX219629B (es) 2004-03-30
IL132259A (en) 2007-05-15
ES2273414T3 (es) 2007-05-01

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