GR890100542A - Συστηματα εκφρασεως για την παρασκευη πολυπεπτιδιων σε προκαρυοτικα κυτταρα. - Google Patents

Συστηματα εκφρασεως για την παρασκευη πολυπεπτιδιων σε προκαρυοτικα κυτταρα.

Info

Publication number
GR890100542A
GR890100542A GR890100542A GR890100542A GR890100542A GR 890100542 A GR890100542 A GR 890100542A GR 890100542 A GR890100542 A GR 890100542A GR 890100542 A GR890100542 A GR 890100542A GR 890100542 A GR890100542 A GR 890100542A
Authority
GR
Greece
Prior art keywords
polypeptide
interest
prokaryotic cells
polypeptides
preparation
Prior art date
Application number
GR890100542A
Other languages
English (en)
Other versions
GR1002113B (en
Inventor
A Gregory Bruce
J Thomas Franceschini
M Timothy Rose
Original Assignee
Oncogen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oncogen filed Critical Oncogen
Publication of GR890100542A publication Critical patent/GR890100542A/el
Publication of GR1002113B publication Critical patent/GR1002113B/el

Links

Landscapes

  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Παρέχονται κασέττες έκφρασης για αυξημένη έκφραση και παραγωγή ενός πολυπεπτιδίου που ενδιαφέρει σε προκαρυωτικά κύτταρα. Οι κασέττες έκφρασης δίνουν παραγωγή του πολυπεπτιδίου που ενδιαφέρει έτσι που ένα τέτοιο πολυπεπτίδιο να μπορεί είτε να εκκρίνεται από το κύτταρο ξενιστή σε μια δραστική διάταξη ή εύκολα να επεξεργάζεται και να επαναγεννάται σε μία λειτουργική κατάσταση. Κατά προτίμηση, το πολυπεπτίδιο που ενδιαφέρει εκφράζεται σαν μία πρωτεϊνη σύντηξης, ιδιαίτερα συντηγμένη προς μία οδηγό αλληλουχία από ένα υψηλά εκφραζόμενο βακτηριακό ή βακτηριοφάγο γονίδιο. Το πολυπεπτίδιο που ενδιαφέρει μπορεί στη συνέχεια να τεμαχίζεται από την οδηγό αλληλουχία και να επαναδιπλώνεται ή να χρησιμοποιείται σαν μία πρωτεϊνη σύντηξης. ω
GR890100542A 1988-09-02 1989-08-31 Expression systems for preparation of polypeptides in prokaryotic cells GR1002113B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US24076888A 1988-09-02 1988-09-02

Publications (2)

Publication Number Publication Date
GR890100542A true GR890100542A (el) 1991-12-30
GR1002113B GR1002113B (en) 1996-02-02

Family

ID=22907871

Family Applications (1)

Application Number Title Priority Date Filing Date
GR890100542A GR1002113B (en) 1988-09-02 1989-08-31 Expression systems for preparation of polypeptides in prokaryotic cells

Country Status (1)

Country Link
GR (1) GR1002113B (el)

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1985003522A1 (en) * 1984-02-08 1985-08-15 Cetus Corporation Monitoring and control systems for recombinant manipulations
US4543329A (en) * 1979-05-31 1985-09-24 Schering Aktiengesellschaft Process for the specific cleavage of protein sequences from proteins
US4565785A (en) * 1978-06-08 1986-01-21 The President And Fellows Of Harvard College Recombinant DNA molecule
EP0173149A1 (de) * 1984-08-21 1986-03-05 Hoechst Aktiengesellschaft Synthetische Regulationsregion
US4582800A (en) * 1982-07-12 1986-04-15 Hoffmann-La Roche Inc. Novel vectors and method for controlling interferon expression
EP0196864A2 (en) * 1985-03-25 1986-10-08 Cetus Corporation Alkaline phosphatase-mediated processing and secretion of recombinant proteins, DNA sequences for use therein and cells transformed using such sequences
EP0241446A2 (en) * 1986-03-27 1987-10-14 Monsanto Company Enhanced protein production in bacteria by employing a novel ribosome binding site
US4711845A (en) * 1984-08-31 1987-12-08 Cetus Corporation Portable temperature-sensitive control cassette

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4565785A (en) * 1978-06-08 1986-01-21 The President And Fellows Of Harvard College Recombinant DNA molecule
US4543329A (en) * 1979-05-31 1985-09-24 Schering Aktiengesellschaft Process for the specific cleavage of protein sequences from proteins
US4582800A (en) * 1982-07-12 1986-04-15 Hoffmann-La Roche Inc. Novel vectors and method for controlling interferon expression
WO1985003522A1 (en) * 1984-02-08 1985-08-15 Cetus Corporation Monitoring and control systems for recombinant manipulations
EP0173149A1 (de) * 1984-08-21 1986-03-05 Hoechst Aktiengesellschaft Synthetische Regulationsregion
US4711845A (en) * 1984-08-31 1987-12-08 Cetus Corporation Portable temperature-sensitive control cassette
EP0196864A2 (en) * 1985-03-25 1986-10-08 Cetus Corporation Alkaline phosphatase-mediated processing and secretion of recombinant proteins, DNA sequences for use therein and cells transformed using such sequences
EP0241446A2 (en) * 1986-03-27 1987-10-14 Monsanto Company Enhanced protein production in bacteria by employing a novel ribosome binding site

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
CHEMICAL ABSTRACTS, vol. 102, no. 3, 21st January 1985, page 188, abstract no. 18845p, Columbus, Ohio, US; L.H. TESSIER et al.: "The influence of mRNA primary and secondary structure on human IFN-gamma gene expression in E. coli", & NUCLEIC ACIDS RES. 1984, 12(20), 7663-75 *
CHEMICAL ABSTRACTS, vol. 103, no. 3, 22nd July 1985, pages 153-154, abstract no. 17753r, Columbus, Ohio, US; T. MIYAKE et al.: "Secretion of human interferon-alpha induced by using secretion vectors containing a promoter and signal sequence of alkaline phosphatase gene of Escherichia coli", & J. BIOCHEM. (TOKYO) 1985, 97(5), 1429-36 *
CHEMICAL ABSTRACTS, vol. 103, no. 7, 19th August 1985, page 616, abstract no. 54444m, Columbus, Ohio, US; F. MARCUS: "Preferential cleavage at aspartyl-prolyl peptide bonds in dilute acid", & INT. J. PEPT. PROTEIN RES. 1985, 25(5), 542-6 *
CHEMICAL ABSTRACTS, vol. 104, no. 7, 17th February 1986, page 149, abstract no. 46697b, Columbus, Ohio, US; & WO-A-85 03 522 (CETUS CORP.) 15-08-1985 *
CHEMICAL ABSTRACTS, vol. 106, no. 17, 27th April 1987, abstract no. 133062a, Columbus, Ohio, US; & EP-A-196 864 (CETUS CORP.) 08-10-1986 *
CHEMICAL ABSTRACTS, vol. 93, no. 20, 17th November 1980, pages 224-225, abstract no. 199455a, Columbus, Ohio, US; G.F.E. SCHERER et al.: "The ribosome binding sites recognized by E. coli ribosomes have regions with signal character in both the leader and protein coding segments", & NUCLEIC ACIDS RES. 1980, 8(17), 3895-907 *
CHEMICAL ABSTRACTS, vol. 99, no. 1, 4th July 1983, page 127, abstract no. 1142d, Columbus, Ohio, US; D.K. HAWLEY et al.: "Compilation and analysis of Escherichia coli promotor DNA sequences", & NUCLEIC ACIDS RES. 1983, 11(8), 2237-55 *
CLAIMS SEARCHED: ALL *
JOURNAL OF BIOTECHNOLOGY, vol. 6, no. 1, July 1987, pages 71-81, Amsterdam, NL; J. BOTTERMAN et al.: "High-level expression of genes under control of Cro translation initiation signals in Escherichia coli" *
THE EMBO JOURNAL, vol. 1, no. 10, December 1982, pages 1217-1224, Oxford, GB; M. ZABEAU et al.: "Enhanced expression of cro-beta-galactosidase fusion proteins under the control of the Pr promoter of bacteriophage lambda" *

Also Published As

Publication number Publication date
GR1002113B (en) 1996-02-02

Similar Documents

Publication Publication Date Title
DK38189A (da) Hidtil ukendte fusionsproteiner indeholdende glutation-s-transferase
FI811100L (fi) Proteinsyntes
ATE198754T1 (de) Antikörper gegen saugetier-interleukin-4 und peptide verwendbar als antigenen für deren herstellung
FI871908A0 (fi) Transformering av trichoderma-moegel.
ES2114539T3 (es) Expresion de polipeptidos recombinados con purificacion mejorada.
AU1575088A (en) Process for the purification of recombinant polypeptides
TR199501134A2 (tr) Memeli ve viral proteinleri asiri ifade edilmesi.
FI852547L (fi) Framstaellning av faktor viii och daertill hoerande produkter.
AU6801487A (en) Method of producing a recombinant protein complex having human factor VIII:C activity
SE9101433D0 (sv) Recombinant dna sequence and its use
ATE364703T1 (de) Herstellung von proteinen aus pro-proteinen durch fusionsproteine abgeleitet von furin oder furinanalogen
ATE79403T1 (de) Herstellung von thrombin-inhibitoren.
DK388789D0 (da) Hybridderivat af humant protein d, dets fremstilling og anvendelse samt dna, som koder derfor, og genstruktur og vaertscelle indeholdende denne dna
DK0434605T3 (da) Ny fremgangsmåde til fremstilling af ikke-fusioneret protein i E.coli
DE3881023D1 (de) Proteine, expressionsvektoren, die fuer solche proteine kodierende dna enthalten und zellen, die solche expressionsvektoren enthalten.
DK0646176T3 (da) Levering og ekspression af et hybridoverfladeprotein på overfladen af grampositive bakterier
NO911557L (no) Fremgangsmaate for fremstilling av fremmedproteiner i streptomyceter.
GR890100542A (el) Συστηματα εκφρασεως για την παρασκευη πολυπεπτιδιων σε προκαρυοτικα κυτταρα.
AU4833385A (en) A synthetic signal sequence for the transport of proteins in expression systems
FI870501A (fi) Menetelmä melanoomaan liittyvän p97-antigeenin antigeenisen sukulaispeptidin tai -proteiinin valmistamiseksi, sekä siinä käyttökelpoiset yhdistelmävirukset, yhdistelmävektorit ja isäntäsolut
Hand et al. Basic mechanisms of cellular secretion
WO1988009809A3 (en) Cell system for expression of recombinant protein without production of virus
DK0725822T3 (da) Fremgangsmåde til rekombinant fremstilling af proteiner i gær
CA2175068A1 (en) The recombinant production of proteins in yeast
SE8901715L (sv) En plasmid dna-konstruktion innehaallande dna-sekvenser