CA2175068A1 - The recombinant production of proteins in yeast - Google Patents

The recombinant production of proteins in yeast

Info

Publication number
CA2175068A1
CA2175068A1 CA002175068A CA2175068A CA2175068A1 CA 2175068 A1 CA2175068 A1 CA 2175068A1 CA 002175068 A CA002175068 A CA 002175068A CA 2175068 A CA2175068 A CA 2175068A CA 2175068 A1 CA2175068 A1 CA 2175068A1
Authority
CA
Canada
Prior art keywords
yeast
proteins
recombinant production
gen
neurohormone
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002175068A
Other languages
French (fr)
Other versions
CA2175068C (en
Inventor
Jurgen Schweden
Claus Bollschweiler
Michael Piontek
Ulrike Weydemann
Gerd Gellissen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF SE
Dynavax GmbH
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE4336810A external-priority patent/DE4336810A1/en
Application filed by Individual filed Critical Individual
Publication of CA2175068A1 publication Critical patent/CA2175068A1/en
Application granted granted Critical
Publication of CA2175068C publication Critical patent/CA2175068C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mycology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A process for the recombinant production of proteins in yeast in which the yeast is transformed with an expression cassette containing the coded structural components: L-A-P-GEN, in which L is a leader sequence of an animal peptidic neurohormone, A is an adaptor generating an alpha helix structure, P is a processing signal and GEN is a structure gene for the desired protein.
CA002175068A 1993-10-28 1994-10-17 The recombinant production of proteins in yeast Expired - Fee Related CA2175068C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE4336810A DE4336810A1 (en) 1993-10-28 1993-10-28 Process for the recombinant production of proteins in yeast
DEP4336810.7 1993-10-28
PCT/EP1994/003409 WO1995011976A1 (en) 1993-10-28 1994-10-17 Process for the recombinant production of proteins in yeast

Publications (2)

Publication Number Publication Date
CA2175068A1 true CA2175068A1 (en) 1995-05-04
CA2175068C CA2175068C (en) 2006-07-18

Family

ID=36694012

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002175068A Expired - Fee Related CA2175068C (en) 1993-10-28 1994-10-17 The recombinant production of proteins in yeast

Country Status (1)

Country Link
CA (1) CA2175068C (en)

Also Published As

Publication number Publication date
CA2175068C (en) 2006-07-18

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