ATE295890T1 - Vektor zur expression von n-terminal verlängerten proteinen in hefezellen - Google Patents
Vektor zur expression von n-terminal verlängerten proteinen in hefezellenInfo
- Publication number
- ATE295890T1 ATE295890T1 AT96941597T AT96941597T ATE295890T1 AT E295890 T1 ATE295890 T1 AT E295890T1 AT 96941597 T AT96941597 T AT 96941597T AT 96941597 T AT96941597 T AT 96941597T AT E295890 T1 ATE295890 T1 AT E295890T1
- Authority
- AT
- Austria
- Prior art keywords
- yeast cells
- expressing
- vector
- terminally extended
- extended proteins
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Mycology (AREA)
- Diabetes (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK144995 | 1995-12-20 | ||
| PCT/DK1996/000542 WO1997022706A1 (en) | 1995-12-20 | 1996-12-18 | Vector for expression of n-terminally extended proteins in yeast cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE295890T1 true ATE295890T1 (de) | 2005-06-15 |
Family
ID=8104944
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT96941597T ATE295890T1 (de) | 1995-12-20 | 1996-12-18 | Vektor zur expression von n-terminal verlängerten proteinen in hefezellen |
Country Status (11)
| Country | Link |
|---|---|
| EP (1) | EP0868523B1 (de) |
| JP (1) | JP4180112B2 (de) |
| CN (1) | CN1125181C (de) |
| AT (1) | ATE295890T1 (de) |
| AU (1) | AU1093597A (de) |
| CA (1) | CA2241367C (de) |
| DE (1) | DE69634757T2 (de) |
| ES (1) | ES2242969T3 (de) |
| IL (1) | IL124939A (de) |
| WO (1) | WO1997022706A1 (de) |
| ZA (1) | ZA9610456B (de) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE253120T1 (de) * | 1996-12-13 | 2003-11-15 | Chiron Corp | Verfahren zur expression von heterologen proteinen in hefe |
| AU9162398A (en) * | 1997-08-22 | 1999-03-16 | Boehringer Mannheim Gmbh | Process for producing insulin precursor proteins |
| PT2270030E (pt) * | 2000-02-28 | 2012-07-24 | Novartis Vaccines & Diagnostic | Expressão heteróloga de proteínas de neisseria |
| WO2002085932A2 (en) * | 2001-04-24 | 2002-10-31 | Innogenetics N.V. | Constructs and methods for expression of recombinant hcv envelope proteins |
| US7595172B2 (en) | 2001-07-24 | 2009-09-29 | Novo Nordisk A/S | Method for making acylated polypeptides |
| DE602005017034D1 (de) * | 2004-06-14 | 2009-11-19 | Novozymes As | Signalpeptid zur herstellung eines polypeptids |
| GB201008682D0 (en) | 2010-05-25 | 2010-07-07 | Vib Vzw | Epitope tag for affinity based applications |
| CN115927360B (zh) * | 2020-05-26 | 2023-07-14 | 北京惠之衡生物科技有限公司 | 密码子优化的门冬胰岛素前体基因、重组载体、基因工程菌及其应用 |
| CN113249241B (zh) * | 2021-06-21 | 2021-10-15 | 奥锐特药业(天津)有限公司 | 一种酿酒酵母蛋白酶缺失菌株的构建及其应用 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3382547D1 (de) * | 1983-01-12 | 1992-05-27 | Chiron Corp | Sekretorische expression in eukaryoten. |
| FR2593518B1 (fr) * | 1985-05-02 | 1989-09-08 | Transgene Sa | Vecteurs d'expression et de secretion de l'hirudine par les levures transformees |
| CA1340772C (en) * | 1987-12-30 | 1999-09-28 | Patricia Tekamp-Olson | Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences |
| DK105489D0 (da) * | 1989-03-03 | 1989-03-03 | Novo Nordisk As | Polypeptid |
| DK82893D0 (da) * | 1993-07-08 | 1993-07-08 | Novo Nordisk As | Peptid |
| US5639642A (en) * | 1994-06-16 | 1997-06-17 | Novo Nordisk A/S | Synthetic leader peptide sequences |
| ZA954983B (en) * | 1994-06-17 | 1996-02-14 | Novo Nordisk As | N-terminally extended proteins expressed in yeast |
-
1996
- 1996-12-12 ZA ZA9610456A patent/ZA9610456B/xx unknown
- 1996-12-18 IL IL124939A patent/IL124939A/en not_active IP Right Cessation
- 1996-12-18 DE DE69634757T patent/DE69634757T2/de not_active Expired - Lifetime
- 1996-12-18 WO PCT/DK1996/000542 patent/WO1997022706A1/en active IP Right Grant
- 1996-12-18 CA CA002241367A patent/CA2241367C/en not_active Expired - Fee Related
- 1996-12-18 AT AT96941597T patent/ATE295890T1/de not_active IP Right Cessation
- 1996-12-18 CN CN96199732A patent/CN1125181C/zh not_active Expired - Lifetime
- 1996-12-18 ES ES96941597T patent/ES2242969T3/es not_active Expired - Lifetime
- 1996-12-18 AU AU10935/97A patent/AU1093597A/en not_active Abandoned
- 1996-12-18 JP JP52231597A patent/JP4180112B2/ja not_active Expired - Lifetime
- 1996-12-18 EP EP96941597A patent/EP0868523B1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| AU1093597A (en) | 1997-07-14 |
| WO1997022706A1 (en) | 1997-06-26 |
| EP0868523B1 (de) | 2005-05-18 |
| EP0868523A1 (de) | 1998-10-07 |
| CN1125181C (zh) | 2003-10-22 |
| CN1207773A (zh) | 1999-02-10 |
| ES2242969T3 (es) | 2005-11-16 |
| ZA9610456B (en) | 1997-06-20 |
| CA2241367A1 (en) | 1997-06-26 |
| IL124939A0 (en) | 1999-01-26 |
| DE69634757D1 (de) | 2005-06-23 |
| CA2241367C (en) | 2007-02-06 |
| JP2000501617A (ja) | 2000-02-15 |
| IL124939A (en) | 2007-06-03 |
| JP4180112B2 (ja) | 2008-11-12 |
| DE69634757T2 (de) | 2006-02-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |