ZA954983B - N-terminally extended proteins expressed in yeast - Google Patents

N-terminally extended proteins expressed in yeast

Info

Publication number
ZA954983B
ZA954983B ZA954983A ZA954983A ZA954983B ZA 954983 B ZA954983 B ZA 954983B ZA 954983 A ZA954983 A ZA 954983A ZA 954983 A ZA954983 A ZA 954983A ZA 954983 B ZA954983 B ZA 954983B
Authority
ZA
South Africa
Prior art keywords
yeast
proteins expressed
terminally extended
extended proteins
terminally
Prior art date
Application number
ZA954983A
Inventor
Knud Vad
Jakob Brandt
Thomas Borglum Kjeldsen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of ZA954983B publication Critical patent/ZA954983B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Diabetes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
ZA954983A 1994-06-17 1995-06-15 N-terminally extended proteins expressed in yeast ZA954983B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DK71294 1994-06-17

Publications (1)

Publication Number Publication Date
ZA954983B true ZA954983B (en) 1996-02-14

Family

ID=8096676

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA954983A ZA954983B (en) 1994-06-17 1995-06-15 N-terminally extended proteins expressed in yeast

Country Status (16)

Country Link
EP (1) EP0765395A1 (en)
JP (1) JP3730255B2 (en)
CN (1) CN1131312C (en)
AU (1) AU2733595A (en)
BR (1) BR9508053A (en)
CA (1) CA2192943A1 (en)
CZ (1) CZ363796A3 (en)
FI (1) FI965030A (en)
HU (1) HUT75840A (en)
IL (1) IL114160A (en)
MX (1) MX9606536A (en)
NO (1) NO965411L (en)
PL (1) PL317761A1 (en)
SG (1) SG47883A1 (en)
WO (1) WO1995035384A1 (en)
ZA (1) ZA954983B (en)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6500645B1 (en) 1994-06-17 2002-12-31 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
ZA9610456B (en) * 1995-12-20 1997-06-20 Novo Nordisk As N-terminally extended proteins expressed in yeast
WO1998025963A1 (en) 1996-12-13 1998-06-18 Chiron Corporation Analysis and separation of platelet-derived growth factor proteins
JP4187796B2 (en) * 1996-12-20 2008-11-26 ノボ ノルディスク アクティーゼルスカブ N-terminal extension protein expressed in yeast
JP2003525570A (en) 1998-01-23 2003-09-02 ノボ ノルディスク アクティーゼルスカブ Method for producing desired polypeptide in yeast
CN1415019A (en) 1999-12-29 2003-04-30 诺沃挪第克公司 Method for making insulin precursors and insulin precursor analogus having improved fermentation yield in yeast
AU2002355160A1 (en) * 2001-07-24 2003-02-17 Novo Nordisk A/S Method for making acylated polypeptides
US7595172B2 (en) 2001-07-24 2009-09-29 Novo Nordisk A/S Method for making acylated polypeptides
US20030082671A1 (en) 2001-07-24 2003-05-01 Thomas Hoeg-Jensen Method for making acylated polypeptides
WO2004085472A1 (en) * 2003-03-27 2004-10-07 Novo Nordisk A/S Method for making human insulin precursors and human insulin
WO2005054291A1 (en) 2003-12-03 2005-06-16 Novo Nordisk A/S Single-chain insulin
ES2368500T3 (en) 2005-08-16 2011-11-17 Novo Nordisk A/S METHOD FOR PRODUCING MATURE INSULIN POLYPEPTIDES.
EP2049149B1 (en) 2006-07-31 2015-04-15 Novo Nordisk A/S Pegylated extended insulins
CN101541830A (en) 2006-09-22 2009-09-23 诺沃-诺迪斯克有限公司 Protease resistant insulin analogues
JP5503968B2 (en) 2006-09-27 2014-05-28 ノボ・ノルデイスク・エー／エス Method for producing mature insulin polypeptide
ES2425413T3 (en) 2006-11-22 2013-10-15 Novo Nordisk A/S Method for preparing activated carboxypeptidase
WO2009010428A1 (en) 2007-07-16 2009-01-22 Novo Nordisk A/S Protease stabilized, pegylated insulin analogues
ES2558930T3 (en) 2007-08-13 2016-02-09 Novo Nordisk A/S Fast-acting insulin analogs
WO2009022013A1 (en) 2007-08-15 2009-02-19 Novo Nordisk A/S Insulin analogues with an acyl and aklylene glycol moiety
JP5721432B2 (en) 2007-08-15 2015-05-20 ノボ・ノルデイスク・エー／エス Insulin having an acyl moiety containing an amino acid-containing alkylene glycol repeating unit
EP2910571B1 (en) 2008-03-18 2016-10-05 Novo Nordisk A/S Protease stabilized, acylated insulin analogues
ES2563553T3 (en) 2008-04-01 2016-03-15 Novo Nordisk A/S Insulin-albumin conjugates
ES2543705T3 (en) 2009-06-26 2015-08-21 Novo Nordisk A/S Preparation containing insulin, nicotinamide and arginine
WO2011064282A1 (en) 2009-11-25 2011-06-03 Novo Nordisk A/S Method for making polypeptides
WO2011161083A1 (en) 2010-06-23 2011-12-29 Novo Nordisk A/S Human insulin containing additional disulfide bonds
US8815798B2 (en) 2010-06-23 2014-08-26 Novo Nordisk A/S Insulin analogues containing additional disulfide bonds
RU2598273C2 (en) 2010-06-23 2016-09-20 Ново Нордиск А/С Insulin derivatives containing additional disulfide bonds
RU2013130374A (en) 2010-12-14 2015-01-20 Ново Нордиск А/С THE PRODUCT CONTAINING INSULIN, NICOTINAMIDE AND AMINO ACID
JP2013545782A (en) 2010-12-14 2013-12-26 ノヴォ ノルディスク アー／エス Fast-acting insulin combined with long-acting insulin
WO2012171994A1 (en) 2011-06-15 2012-12-20 Novo Nordisk A/S Multi substituted insulins
WO2013186138A1 (en) 2012-06-14 2013-12-19 Novo Nordisk A/S Preparation comprising insulin, nicotinamide and arginine
JP6735561B2 (en) 2012-12-03 2020-08-05 メルク・シャープ・アンド・ドーム・コーポレーションＭｅｒｃｋ Ｓｈａｒｐ ＆ Ｄｏｈｍｅ Ｃｏｒｐ． O-glycosylated carboxy-terminal portion (CTP) peptide-based insulin and insulin analogs
JP2016521701A (en) 2013-06-07 2016-07-25 ノヴォ ノルディスク アー／エス Methods for making mature insulin polypeptides
EP3110834B1 (en) 2014-02-28 2018-11-07 Novo Nordisk A/S Mating factor alpha pro-peptide variants
US11208452B2 (en) 2015-06-02 2021-12-28 Novo Nordisk A/S Insulins with polar recombinant extensions
US20190010206A1 (en) 2015-08-25 2019-01-10 Novo Nordisk A/S Novel Insulin Derivatives and the Medical Uses Hereof
TW201717998A (en) 2015-08-25 2017-06-01 諾佛 儂迪克股份有限公司 Novel insulin derivatives and the medical uses hereof
US20180244743A1 (en) 2015-08-25 2018-08-30 Novo Nordisk A/S Novel Insulin Derivatives and the Medical Uses Hereof
MA45113A (en) * 2016-05-24 2019-04-10 Novo Nordisk As MIC-1 COMPOUNDS AND THEIR USE
MX2020001434A (en) 2017-08-17 2020-03-20 Novo Nordisk As Novel acylated insulin analogues and uses thereof.
CN112105635A (en) * 2018-06-18 2020-12-18 联合化学实验室有限公司 Leader sequences for higher expression of recombinant proteins
BR112022009510A2 (en) 2019-12-11 2022-08-16 Novo Nordisk As INSULIN ANALOG, PHARMACEUTICAL COMPOSITION, AND, METHOD OF TREATMENT, PREVENTION, OR ALLIEF OF A DISEASE OR DISORDER OR CONDITION
WO2023144240A1 (en) 2022-01-26 2023-08-03 Novo Nordisk Research Centre Oxford Limited Glucose sensitive insulin derivatives and uses thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2593518B1 (en) * 1985-05-02 1989-09-08 Transgene Sa VECTORS FOR THE EXPRESSION AND SECRETION OF HIRUDIN BY TRANSFORMED YEASTS
US5010182A (en) * 1987-07-28 1991-04-23 Chiron Corporation DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
CA1340772C (en) * 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
DK105489D0 (en) * 1989-03-03 1989-03-03 Novo Nordisk As POLYPEPTIDE
DK300090D0 (en) * 1990-12-19 1990-12-19 Novo Nordisk As PROCEDURE FOR PREPARING LEADER SEQUENCES
WO1992013951A1 (en) * 1991-02-04 1992-08-20 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of human serum albumin in methylotrophic yeast cells

Also Published As

Publication number Publication date
CN1152942A (en) 1997-06-25
WO1995035384A1 (en) 1995-12-28
EP0765395A1 (en) 1997-04-02
FI965030A0 (en) 1996-12-16
HUT75840A (en) 1997-05-28
AU2733595A (en) 1996-01-15
FI965030A (en) 1997-02-14
BR9508053A (en) 1997-08-12
PL317761A1 (en) 1997-04-28
JPH10501695A (en) 1998-02-17
IL114160A (en) 2006-12-31
NO965411L (en) 1997-02-14
JP3730255B2 (en) 2005-12-21
CA2192943A1 (en) 1995-12-28
NO965411D0 (en) 1996-12-16
CZ363796A3 (en) 1997-05-14
HU9603476D0 (en) 1997-02-28
SG47883A1 (en) 1998-04-17
IL114160A0 (en) 1995-10-31
CN1131312C (en) 2003-12-17
MX9606536A (en) 1997-03-29

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