NO965411L - N-terminally extended proteins expressed in yeast - Google Patents

N-terminally extended proteins expressed in yeast

Info

Publication number
NO965411L
NO965411L NO965411A NO965411A NO965411L NO 965411 L NO965411 L NO 965411L NO 965411 A NO965411 A NO 965411A NO 965411 A NO965411 A NO 965411A NO 965411 L NO965411 L NO 965411L
Authority
NO
Norway
Prior art keywords
glu
asp
yeast
arg
amino acids
Prior art date
Application number
NO965411A
Other languages
Norwegian (no)
Other versions
NO965411D0 (en
Inventor
Knud Vad
Jakob Brandt
Thomas Boerglum Kjeldsen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of NO965411D0 publication Critical patent/NO965411D0/en
Publication of NO965411L publication Critical patent/NO965411L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Abstract

Foreliggende oppfinnelse ved-rører polypeptider uttrykt og be-arbeidet i gjær, en DNA-konstruksjon som omfatter en DNA-sekvens som koder for et polypeptid med den følgende struktur: signalpeptid-lederpeptid-XXXheterologt protein, hvor Xer Lys eller Arg; Xer Lys eller Arg;ogdefinerer til sammen et gjærprosesseringssete; Xer Glu eller Asp; Xer en sekvens av aminosyrer med den følgende struktur:. (A-B)hvor A er Glu eller Asp,. B er Ala, Val, Leu eller Pro, og. n er 0 eller et helt tall fra 1 til 5, og når n>2, er hver A og B like eller forskjellige fra den eller de øvrige A (er) og B(er), eller Xer en sekvens av aminosyrer med den følgende struktur: (C)hvor C er Glu eller Asp, og m er 0 eller et helt tall fra 1 til 5;er en peptidbinding eller er én eller flere aminosyrer som kan være like eller forskjellige; Xer en peptidbinding eller en aminosyrerest valgt fra gruppen bestående av Pro, Asp, Thr, Ser, Glu, Ala og Gly; oger Lys eller Arg.The present invention relates to polypeptides expressed and processed in yeast, a DNA construct comprising a DNA sequence encoding a polypeptide having the following structure: signal peptide leader peptide XXX heterologous protein, wherein X is Lys or Arg; Xer Light or Arg; and together define a yeast processing site; Xer Glu or Asp; X is a sequence of amino acids having the following structure:. (A-B) where A is Glu or Asp ,. B is Ala, Val, Leu or Pro, and. n is 0 or an integer from 1 to 5, and when n> 2, each A and B is the same or different from the other A (s) and B (s), or X is a sequence of amino acids having the following structure: (C) wherein C is Glu or Asp, and m is 0 or an integer from 1 to 5, is a peptide bond or is one or more amino acids which may be the same or different; X is a peptide bond or an amino acid residue selected from the group consisting of Pro, Asp, Thr, Ser, Glu, Ala and Gly; and Light or Arg.

NO965411A 1994-06-17 1996-12-16 N-terminally extended proteins expressed in yeast NO965411L (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK71294 1994-06-17
PCT/DK1995/000250 WO1995035384A1 (en) 1994-06-17 1995-06-16 N-terminally extended proteins expressed in yeast

Publications (2)

Publication Number Publication Date
NO965411D0 NO965411D0 (en) 1996-12-16
NO965411L true NO965411L (en) 1997-02-14

Family

ID=8096676

Family Applications (1)

Application Number Title Priority Date Filing Date
NO965411A NO965411L (en) 1994-06-17 1996-12-16 N-terminally extended proteins expressed in yeast

Country Status (16)

Country Link
EP (1) EP0765395A1 (en)
JP (1) JP3730255B2 (en)
CN (1) CN1131312C (en)
AU (1) AU2733595A (en)
BR (1) BR9508053A (en)
CA (1) CA2192943A1 (en)
CZ (1) CZ363796A3 (en)
FI (1) FI965030A (en)
HU (1) HUT75840A (en)
IL (1) IL114160A (en)
MX (1) MX9606536A (en)
NO (1) NO965411L (en)
PL (1) PL317761A1 (en)
SG (1) SG47883A1 (en)
WO (1) WO1995035384A1 (en)
ZA (1) ZA954983B (en)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6500645B1 (en) 1994-06-17 2002-12-31 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
ZA9610456B (en) * 1995-12-20 1997-06-20 Novo Nordisk As N-terminally extended proteins expressed in yeast
US6083910A (en) 1996-12-13 2000-07-04 Chiron Corporation Therapeutic uses of resolved intact or clipped native-sequence PDGF-BB dimers
WO1998028429A1 (en) * 1996-12-20 1998-07-02 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
EP1049790A1 (en) * 1998-01-23 2000-11-08 Novo Nordisk A/S Process for making desired polypeptides in yeast
KR20030004315A (en) 1999-12-29 2003-01-14 노보 노르디스크 에이/에스 Method for making insulin precursors and insulin precursor analogues having improved fermentation yield in yeast
JP2005503141A (en) * 2001-07-24 2005-02-03 ノボ　ノルディスク　アクティーゼルスカブ Methods for making acylated polypeptides
US20030082671A1 (en) 2001-07-24 2003-05-01 Thomas Hoeg-Jensen Method for making acylated polypeptides
US7595172B2 (en) 2001-07-24 2009-09-29 Novo Nordisk A/S Method for making acylated polypeptides
WO2004085472A1 (en) * 2003-03-27 2004-10-07 Novo Nordisk A/S Method for making human insulin precursors and human insulin
ATE517119T1 (en) 2003-12-03 2011-08-15 Novo Nordisk As SINGLE CHAIN INSULIN
US9056921B2 (en) 2005-08-16 2015-06-16 Novo Nordisk A/S Method for making mature insulin polypeptides
CN101573133B (en) * 2006-07-31 2014-08-27 诺沃-诺迪斯克有限公司 PEGylated, extended insulins
EP2074141B1 (en) 2006-09-22 2016-08-10 Novo Nordisk A/S Protease resistant insulin analogues
WO2008037735A1 (en) 2006-09-27 2008-04-03 Novo Nordisk A/S Method for making maturated insulin polypeptides
WO2008062010A2 (en) 2006-11-22 2008-05-29 Novo Nordisk A/S Method for making activated carboxypeptidases
JP5688969B2 (en) 2007-07-16 2015-03-25 ノボ・ノルデイスク・エー／エス Pegylated insulin analogues are stable against proteases
ES2558930T3 (en) 2007-08-13 2016-02-09 Novo Nordisk A/S Fast-acting insulin analogs
CA2695970A1 (en) 2007-08-15 2009-02-19 Novo Nordisk A\S Insulin analogues with an acyl and aklylene glycol moiety
EP2178910B1 (en) 2007-08-15 2014-10-08 Novo Nordisk A/S Insulins with an acyl moiety comprising repeating units of alkylene glycol containing amino acids
EP2910571B1 (en) 2008-03-18 2016-10-05 Novo Nordisk A/S Protease stabilized, acylated insulin analogues
WO2009121884A1 (en) 2008-04-01 2009-10-08 Novo Nordisk A/S Insulin albumin conjugates
WO2010149772A1 (en) 2009-06-26 2010-12-29 Novo Nordisk A/S Preparation comprising insulin, nicotinamide and an amino acid
EP2504355B1 (en) 2009-11-25 2015-07-22 Novo Nordisk A/S Method for making polypeptides
WO2011161125A1 (en) 2010-06-23 2011-12-29 Novo Nordisk A/S Insulin derivatives containing additional disulfide bonds
CN102933599A (en) 2010-06-23 2013-02-13 诺沃—诺迪斯克有限公司 Human insulin containing additional disulfide bonds
EP2585484A1 (en) 2010-06-23 2013-05-01 Novo Nordisk A/S Insulin analogues containing additional disulfide bonds
CN103249427A (en) 2010-12-14 2013-08-14 诺沃—诺迪斯克有限公司 Fast-acting insulin in combination with long-acting insulin
RU2013130374A (en) 2010-12-14 2015-01-20 Ново Нордиск А/С THE PRODUCT CONTAINING INSULIN, NICOTINAMIDE AND AMINO ACID
US9260503B2 (en) 2011-06-15 2016-02-16 Novo Nordisk A/S Multi-substituted insulins
WO2013186138A1 (en) 2012-06-14 2013-12-19 Novo Nordisk A/S Preparation comprising insulin, nicotinamide and arginine
WO2014088836A1 (en) 2012-12-03 2014-06-12 Merck Sharp & Dohme Corp. O-glycosylated carboxy terminal portion (ctp) peptide-based insulin and insulin analogues
EP3004156A1 (en) 2013-06-07 2016-04-13 Novo Nordisk A/S Method for making mature insulin polypeptides
ES2784603T3 (en) 2015-06-02 2020-09-29 Novo Nordisk As Insulins with recombinant polar extensions
JP2018531900A (en) 2015-08-25 2018-11-01 ノヴォ ノルディスク アー／エス Novel insulin derivatives and their medical use
US20190010206A1 (en) 2015-08-25 2019-01-10 Novo Nordisk A/S Novel Insulin Derivatives and the Medical Uses Hereof
JP2018531899A (en) 2015-08-25 2018-11-01 ノヴォ ノルディスク アー／エス Novel insulin derivatives and their medical use
US20190248852A1 (en) * 2016-05-24 2019-08-15 Novo Nordisk A/S MIC-1 Compounds and Use Thereof
AU2018317810A1 (en) 2017-08-17 2020-03-19 Novo Nordisk A/S Novel acylated insulin analogues and uses thereof
US20210230659A1 (en) * 2018-06-18 2021-07-29 Unichem Laboratories Ltd Leader Sequence for Higher Expression of Recombinant Proteins
EP4073097A1 (en) 2019-12-11 2022-10-19 Novo Nordisk A/S Novel insulin analogues and uses thereof
WO2023144240A1 (en) 2022-01-26 2023-08-03 Novo Nordisk Research Centre Oxford Limited Glucose sensitive insulin derivatives and uses thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2593518B1 (en) * 1985-05-02 1989-09-08 Transgene Sa VECTORS FOR THE EXPRESSION AND SECRETION OF HIRUDIN BY TRANSFORMED YEASTS
US5010182A (en) * 1987-07-28 1991-04-23 Chiron Corporation DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
CA1340772C (en) * 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
DK105489D0 (en) * 1989-03-03 1989-03-03 Novo Nordisk As POLYPEPTIDE
DK300090D0 (en) * 1990-12-19 1990-12-19 Novo Nordisk As PROCEDURE FOR PREPARING LEADER SEQUENCES
WO1992013951A1 (en) * 1991-02-04 1992-08-20 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of human serum albumin in methylotrophic yeast cells

Also Published As

Publication number Publication date
IL114160A0 (en) 1995-10-31
ZA954983B (en) 1996-02-14
BR9508053A (en) 1997-08-12
JP3730255B2 (en) 2005-12-21
JPH10501695A (en) 1998-02-17
PL317761A1 (en) 1997-04-28
HUT75840A (en) 1997-05-28
HU9603476D0 (en) 1997-02-28
FI965030A (en) 1997-02-14
SG47883A1 (en) 1998-04-17
NO965411D0 (en) 1996-12-16
CZ363796A3 (en) 1997-05-14
CA2192943A1 (en) 1995-12-28
WO1995035384A1 (en) 1995-12-28
CN1131312C (en) 2003-12-17
EP0765395A1 (en) 1997-04-02
CN1152942A (en) 1997-06-25
AU2733595A (en) 1996-01-15
FI965030A0 (en) 1996-12-16
MX9606536A (en) 1997-03-29
IL114160A (en) 2006-12-31

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