FI965030A0 - N-terminally extended proteins expressed in yeast - Google Patents

N-terminally extended proteins expressed in yeast

Info

Publication number
FI965030A0
FI965030A0 FI965030A FI965030A FI965030A0 FI 965030 A0 FI965030 A0 FI 965030A0 FI 965030 A FI965030 A FI 965030A FI 965030 A FI965030 A FI 965030A FI 965030 A0 FI965030 A0 FI 965030A0
Authority
FI
Finland
Prior art keywords
yeast
proteins expressed
terminally extended
extended proteins
terminally
Prior art date
Application number
FI965030A
Other languages
Finnish (fi)
Swedish (sv)
Other versions
FI965030A (en
Inventor
Knud Vad
Jakob Brandt
Thomas Boerglum Kjeldsen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of FI965030A0 publication Critical patent/FI965030A0/en
Publication of FI965030A publication Critical patent/FI965030A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
FI965030A 1994-06-17 1996-12-16 N-terminally elongated proteins expressed in yeast FI965030A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK71294 1994-06-17
PCT/DK1995/000250 WO1995035384A1 (en) 1994-06-17 1995-06-16 N-terminally extended proteins expressed in yeast

Publications (2)

Publication Number Publication Date
FI965030A0 true FI965030A0 (en) 1996-12-16
FI965030A FI965030A (en) 1997-02-14

Family

ID=8096676

Family Applications (1)

Application Number Title Priority Date Filing Date
FI965030A FI965030A (en) 1994-06-17 1996-12-16 N-terminally elongated proteins expressed in yeast

Country Status (16)

Country Link
EP (1) EP0765395A1 (en)
JP (1) JP3730255B2 (en)
CN (1) CN1131312C (en)
AU (1) AU2733595A (en)
BR (1) BR9508053A (en)
CA (1) CA2192943A1 (en)
CZ (1) CZ363796A3 (en)
FI (1) FI965030A (en)
HU (1) HUT75840A (en)
IL (1) IL114160A (en)
MX (1) MX9606536A (en)
NO (1) NO965411L (en)
PL (1) PL317761A1 (en)
SG (1) SG47883A1 (en)
WO (1) WO1995035384A1 (en)
ZA (1) ZA954983B (en)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6500645B1 (en) 1994-06-17 2002-12-31 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
ZA9610456B (en) * 1995-12-20 1997-06-20 Novo Nordisk As N-terminally extended proteins expressed in yeast
EP1365028B1 (en) 1996-12-13 2009-03-18 Novartis Vaccines and Diagnostics, Inc. Method for expression of PDGF or IGF proteins in yeast
JP4187796B2 (en) * 1996-12-20 2008-11-26 ノボ ノルディスク アクティーゼルスカブ N-terminal extension protein expressed in yeast
KR20010034305A (en) * 1998-01-23 2001-04-25 한센 핀 베네드 Process for making desired polypeptides in yeast
AU1995701A (en) 1999-12-29 2001-07-16 Novo Nordisk A/S Method for making insulin precursors and insulin precursor analogues having improved fermentation yield in yeast
US20030082671A1 (en) 2001-07-24 2003-05-01 Thomas Hoeg-Jensen Method for making acylated polypeptides
WO2003010185A2 (en) * 2001-07-24 2003-02-06 Novo Nordisk A/S Method for making acylated polypeptides
US7595172B2 (en) 2001-07-24 2009-09-29 Novo Nordisk A/S Method for making acylated polypeptides
WO2004085472A1 (en) * 2003-03-27 2004-10-07 Novo Nordisk A/S Method for making human insulin precursors and human insulin
EP1692168B1 (en) 2003-12-03 2011-07-20 Novo Nordisk A/S Single-chain insulin
US9056921B2 (en) 2005-08-16 2015-06-16 Novo Nordisk A/S Method for making mature insulin polypeptides
CN101573133B (en) * 2006-07-31 2014-08-27 诺沃-诺迪斯克有限公司 PEGylated, extended insulins
BRPI0717098B8 (en) 2006-09-22 2021-05-25 Novo Nordisk As protease-resistant human insulin analogues, pharmaceutical composition and process for their preparation
JP5503968B2 (en) 2006-09-27 2014-05-28 ノボ・ノルデイスク・エー／エス Method for producing mature insulin polypeptide
WO2008062010A2 (en) 2006-11-22 2008-05-29 Novo Nordisk A/S Method for making activated carboxypeptidases
WO2009010428A1 (en) 2007-07-16 2009-01-22 Novo Nordisk A/S Protease stabilized, pegylated insulin analogues
US8575096B2 (en) 2007-08-13 2013-11-05 Novo Nordisk A/S Rapid acting insulin analogues
EP2708554A1 (en) 2007-08-15 2014-03-19 Novo Nordisk A/S Insulin analogues with an acyl and alkylene glycol moiety
EP2178910B1 (en) 2007-08-15 2014-10-08 Novo Nordisk A/S Insulins with an acyl moiety comprising repeating units of alkylene glycol containing amino acids
KR20110004366A (en) 2008-03-18 2011-01-13 노보 노르디스크 에이/에스 Protease stabilized, acylated insulin analogues
WO2009121884A1 (en) 2008-04-01 2009-10-08 Novo Nordisk A/S Insulin albumin conjugates
PL2612677T3 (en) 2009-06-26 2015-10-30 Novo Nordisk As Preparation comprising insulin, nicotinamide and arginine
US8815541B2 (en) 2009-11-25 2014-08-26 Novo Nordisk A/S Method for making polypeptides
WO2011161124A1 (en) 2010-06-23 2011-12-29 Novo Nordisk A/S Insulin analogues containing additional disulfide bonds
CA2802510A1 (en) 2010-06-23 2011-12-29 Novo Nordisk A/S Insulin derivatives containing additional disulfide bonds
EP2585483A1 (en) 2010-06-23 2013-05-01 Novo Nordisk A/S Human insulin containing additional disulfide bonds
EP2651446A1 (en) 2010-12-14 2013-10-23 Novo Nordisk A/S Preparation comprising insulin, nicotinamide and an amino acid
JP2013545782A (en) 2010-12-14 2013-12-26 ノヴォ ノルディスク アー／エス Fast-acting insulin combined with long-acting insulin
CN103596584B (en) 2011-06-15 2016-12-14 诺沃—诺迪斯克有限公司 Polysubstituted insulin
WO2013186138A1 (en) 2012-06-14 2013-12-19 Novo Nordisk A/S Preparation comprising insulin, nicotinamide and arginine
US9707276B2 (en) 2012-12-03 2017-07-18 Merck Sharp & Dohme Corp. O-glycosylated carboxy terminal portion (CTP) peptide-based insulin and insulin analogues
EP3004156A1 (en) 2013-06-07 2016-04-13 Novo Nordisk A/S Method for making mature insulin polypeptides
US20160376330A1 (en) 2014-02-28 2016-12-29 Novo Nordisk A/S Mating factor alpha pro-peptide variants
ES2784603T3 (en) 2015-06-02 2020-09-29 Novo Nordisk As Insulins with recombinant polar extensions
US20180244743A1 (en) 2015-08-25 2018-08-30 Novo Nordisk A/S Novel Insulin Derivatives and the Medical Uses Hereof
CN108026156A (en) 2015-08-25 2018-05-11 诺和诺德股份有限公司 Novel insulin derivates and its medical usage
WO2017032798A1 (en) 2015-08-25 2017-03-02 Novo Nordisk A/S Novel insulin derivatives and the medical uses hereof
CN109414471A (en) * 2016-05-24 2019-03-01 诺和诺德股份有限公司 MIC-1 compound and application thereof
MA49896A (en) 2017-08-17 2020-06-24 Novo Nordisk As NEW ACYLATED INSULIN ANALOGUES AND THEIR USES
US20210230659A1 (en) * 2018-06-18 2021-07-29 Unichem Laboratories Ltd Leader Sequence for Higher Expression of Recombinant Proteins
FI4073097T3 (en) 2019-12-11 2024-08-13 Novo Nordisk As Novel insulin analogues and uses thereof
WO2023144240A1 (en) 2022-01-26 2023-08-03 Novo Nordisk Research Centre Oxford Limited Glucose sensitive insulin derivatives and uses thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2593518B1 (en) * 1985-05-02 1989-09-08 Transgene Sa VECTORS FOR THE EXPRESSION AND SECRETION OF HIRUDIN BY TRANSFORMED YEASTS
US5010182A (en) * 1987-07-28 1991-04-23 Chiron Corporation DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
CA1340772C (en) * 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
DK105489D0 (en) * 1989-03-03 1989-03-03 Novo Nordisk As POLYPEPTIDE
DK300090D0 (en) * 1990-12-19 1990-12-19 Novo Nordisk As PROCEDURE FOR PREPARING LEADER SEQUENCES
WO1992013951A1 (en) * 1991-02-04 1992-08-20 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of human serum albumin in methylotrophic yeast cells

Also Published As

Publication number Publication date
AU2733595A (en) 1996-01-15
CN1131312C (en) 2003-12-17
EP0765395A1 (en) 1997-04-02
SG47883A1 (en) 1998-04-17
ZA954983B (en) 1996-02-14
JP3730255B2 (en) 2005-12-21
PL317761A1 (en) 1997-04-28
HUT75840A (en) 1997-05-28
NO965411L (en) 1997-02-14
CZ363796A3 (en) 1997-05-14
NO965411D0 (en) 1996-12-16
BR9508053A (en) 1997-08-12
WO1995035384A1 (en) 1995-12-28
FI965030A (en) 1997-02-14
IL114160A (en) 2006-12-31
MX9606536A (en) 1997-03-29
CN1152942A (en) 1997-06-25
HU9603476D0 (en) 1997-02-28
IL114160A0 (en) 1995-10-31
CA2192943A1 (en) 1995-12-28
JPH10501695A (en) 1998-02-17

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