NO965411L - N-terminalt forlengede proteiner uttrykt i gjær - Google Patents

N-terminalt forlengede proteiner uttrykt i gjær

Info

Publication number
NO965411L
NO965411L NO965411A NO965411A NO965411L NO 965411 L NO965411 L NO 965411L NO 965411 A NO965411 A NO 965411A NO 965411 A NO965411 A NO 965411A NO 965411 L NO965411 L NO 965411L
Authority
NO
Norway
Prior art keywords
glu
asp
yeast
arg
amino acids
Prior art date
Application number
NO965411A
Other languages
English (en)
Other versions
NO965411D0 (no
Inventor
Knud Vad
Jakob Brandt
Thomas Boerglum Kjeldsen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of NO965411D0 publication Critical patent/NO965411D0/no
Publication of NO965411L publication Critical patent/NO965411L/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Foreliggende oppfinnelse ved-rører polypeptider uttrykt og be-arbeidet i gjær, en DNA-konstruksjon som omfatter en DNA-sekvens som koder for et polypeptid med den følgende struktur: signalpeptid-lederpeptid-XXXheterologt protein, hvor Xer Lys eller Arg; Xer Lys eller Arg;ogdefinerer til sammen et gjærprosesseringssete; Xer Glu eller Asp; Xer en sekvens av aminosyrer med den følgende struktur:. (A-B)hvor A er Glu eller Asp,. B er Ala, Val, Leu eller Pro, og. n er 0 eller et helt tall fra 1 til 5, og når n>2, er hver A og B like eller forskjellige fra den eller de øvrige A (er) og B(er), eller Xer en sekvens av aminosyrer med den følgende struktur: (C)hvor C er Glu eller Asp, og m er 0 eller et helt tall fra 1 til 5;er en peptidbinding eller er én eller flere aminosyrer som kan være like eller forskjellige; Xer en peptidbinding eller en aminosyrerest valgt fra gruppen bestående av Pro, Asp, Thr, Ser, Glu, Ala og Gly; oger Lys eller Arg.
NO965411A 1994-06-17 1996-12-16 N-terminalt forlengede proteiner uttrykt i gjær NO965411L (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK71294 1994-06-17
PCT/DK1995/000250 WO1995035384A1 (en) 1994-06-17 1995-06-16 N-terminally extended proteins expressed in yeast

Publications (2)

Publication Number Publication Date
NO965411D0 NO965411D0 (no) 1996-12-16
NO965411L true NO965411L (no) 1997-02-14

Family

ID=8096676

Family Applications (1)

Application Number Title Priority Date Filing Date
NO965411A NO965411L (no) 1994-06-17 1996-12-16 N-terminalt forlengede proteiner uttrykt i gjær

Country Status (16)

Country Link
EP (1) EP0765395A1 (no)
JP (1) JP3730255B2 (no)
CN (1) CN1131312C (no)
AU (1) AU2733595A (no)
BR (1) BR9508053A (no)
CA (1) CA2192943A1 (no)
CZ (1) CZ363796A3 (no)
FI (1) FI965030A7 (no)
HU (1) HUT75840A (no)
IL (1) IL114160A (no)
MX (1) MX9606536A (no)
NO (1) NO965411L (no)
PL (1) PL317761A1 (no)
SG (1) SG47883A1 (no)
WO (1) WO1995035384A1 (no)
ZA (1) ZA954983B (no)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6500645B1 (en) 1994-06-17 2002-12-31 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
ZA9610456B (en) * 1995-12-20 1997-06-20 Novo Nordisk As N-terminally extended proteins expressed in yeast
WO1998025963A1 (en) 1996-12-13 1998-06-18 Chiron Corporation Analysis and separation of platelet-derived growth factor proteins
JP4187796B2 (ja) * 1996-12-20 2008-11-26 ノボ ノルディスク アクティーゼルスカブ 酵母で発現されたｎ末端伸長タンパク質
JP2003525570A (ja) * 1998-01-23 2003-09-02 ノボ ノルディスク アクティーゼルスカブ 酵母において所望のポリペプチドを産生する方法
KR20030004315A (ko) 1999-12-29 2003-01-14 노보 노르디스크 에이/에스 효모에서 개선된 발효 수율을 갖는 인슐린 선구물질 및인슐린 선구물질 유사체의 제조 방법
EP1421102A2 (en) * 2001-07-24 2004-05-26 Novo Nordisk A/S Method for making acylated polypeptides
US20030082671A1 (en) 2001-07-24 2003-05-01 Thomas Hoeg-Jensen Method for making acylated polypeptides
US7595172B2 (en) 2001-07-24 2009-09-29 Novo Nordisk A/S Method for making acylated polypeptides
WO2004085472A1 (en) * 2003-03-27 2004-10-07 Novo Nordisk A/S Method for making human insulin precursors and human insulin
JP5697831B2 (ja) 2003-12-03 2015-04-08 ノヴォ ノルディスク アー／エス 単鎖インシュリン
CN101243190B (zh) 2005-08-16 2015-05-13 诺沃-诺迪斯克有限公司 制备成熟胰岛素多肽的方法
EP2049149B1 (en) 2006-07-31 2015-04-15 Novo Nordisk A/S Pegylated extended insulins
EP2404934A1 (en) 2006-09-22 2012-01-11 Novo Nordisk A/S Protease resistant insulin analogues
US8518668B2 (en) 2006-09-27 2013-08-27 Novo Nordisk A/S Method for making maturated insulin polypeptides in a fungal cell
WO2008062010A2 (en) 2006-11-22 2008-05-29 Novo Nordisk A/S Method for making activated carboxypeptidases
JP5688969B2 (ja) 2007-07-16 2015-03-25 ノボ・ノルデイスク・エー／エス プロテアーゼに対して安定しているペグ化インスリンアナログ
ES2558930T3 (es) 2007-08-13 2016-02-09 Novo Nordisk A/S Análogos de la insulina de acción rápida
KR20100053561A (ko) 2007-08-15 2010-05-20 노보 노르디스크 에이/에스 아실 및 알킬렌 글리콜 부분을 갖는 인슐린 유사체
CN101784563B (zh) 2007-08-15 2015-02-04 诺沃-诺迪斯克有限公司 具有包括含亚烷基二醇的氨基酸的重复单位的酰基部分的胰岛素
MX2010009850A (es) 2008-03-18 2010-09-30 Novo Nordisk As Analogos de insulina acilados y etabilizados contra proteasas.
ES2563553T3 (es) 2008-04-01 2016-03-15 Novo Nordisk A/S Conjugados de insulina-albúmina
KR101775000B1 (ko) 2009-06-26 2017-09-04 노보 노르디스크 에이/에스 인슐린, 니코틴아미드 및 아미노산을 포함하는 제제
CN102639559B (zh) 2009-11-25 2015-04-29 诺沃—诺迪斯克有限公司 用于制备多肽的方法
WO2011161125A1 (en) 2010-06-23 2011-12-29 Novo Nordisk A/S Insulin derivatives containing additional disulfide bonds
EP2585484A1 (en) 2010-06-23 2013-05-01 Novo Nordisk A/S Insulin analogues containing additional disulfide bonds
EP2585483A1 (en) 2010-06-23 2013-05-01 Novo Nordisk A/S Human insulin containing additional disulfide bonds
US20130331320A1 (en) 2010-12-14 2013-12-12 Novo Nordisk A/S Fast-acting insulin in combination with long-acting insulin
CN103328006A (zh) 2010-12-14 2013-09-25 诺沃—诺迪斯克有限公司 包含胰岛素、烟酰胺和氨基酸的制剂
CN103596584B (zh) 2011-06-15 2016-12-14 诺沃—诺迪斯克有限公司 多取代的胰岛素
WO2013186138A1 (en) 2012-06-14 2013-12-19 Novo Nordisk A/S Preparation comprising insulin, nicotinamide and arginine
JP6735561B2 (ja) 2012-12-03 2020-08-05 メルク・シャープ・アンド・ドーム・コーポレーションＭｅｒｃｋ Ｓｈａｒｐ ＆ Ｄｏｈｍｅ Ｃｏｒｐ． Ｏ−グリコシル化カルボキシ末端部分（ｃｔｐ）ペプチド系のインスリンおよびインスリン類似体
JP2016521701A (ja) 2013-06-07 2016-07-25 ノヴォ ノルディスク アー／エス 成熟インスリンポリペプチドを作製するための方法
CN120060321A (zh) 2014-02-28 2025-05-30 诺和诺德股份有限公司 交配因子α原肽变体
ES2784603T3 (es) 2015-06-02 2020-09-29 Novo Nordisk As Insulinas con extensiones recombinantes polares
WO2017032795A1 (en) 2015-08-25 2017-03-02 Novo Nordisk A/S Novel insulin derivatives and the medical uses hereof
CA2996455A1 (en) 2015-08-25 2017-03-02 Novo Nordisk A/S Novel insulin derivatives and the medical uses hereof
EP3341402A1 (en) 2015-08-25 2018-07-04 Novo Nordisk A/S Novel insulin derivatives and the medical uses hereof
MA45113A (fr) * 2016-05-24 2019-04-10 Novo Nordisk As Composés mic-1 et leur utilisation
PE20211264A1 (es) 2017-08-17 2021-07-15 Novo Nordisk As Analogos de insulina acilados novedosos y usos de estos
US20210230659A1 (en) * 2018-06-18 2021-07-29 Unichem Laboratories Ltd Leader Sequence for Higher Expression of Recombinant Proteins
CN110305223B (zh) * 2019-06-26 2022-05-13 重庆派金生物科技有限公司 重组串联融合蛋白制备目标多肽的方法
FI4073097T3 (fi) 2019-12-11 2024-08-13 Novo Nordisk As Uusia insuliinianalogeja ja niiden käyttötapoja
WO2023144240A1 (en) 2022-01-26 2023-08-03 Novo Nordisk Research Centre Oxford Limited Glucose sensitive insulin derivatives and uses thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2593518B1 (fr) * 1985-05-02 1989-09-08 Transgene Sa Vecteurs d'expression et de secretion de l'hirudine par les levures transformees
US5010182A (en) * 1987-07-28 1991-04-23 Chiron Corporation DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
CA1340772C (en) * 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
DK105489D0 (da) * 1989-03-03 1989-03-03 Novo Nordisk As Polypeptid
DK300090D0 (da) * 1990-12-19 1990-12-19 Novo Nordisk As Fremgangsmaade til fremstilling af leadersekvenser
WO1992013951A1 (en) * 1991-02-04 1992-08-20 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of human serum albumin in methylotrophic yeast cells

Also Published As

Publication number Publication date
JP3730255B2 (ja) 2005-12-21
SG47883A1 (en) 1998-04-17
JPH10501695A (ja) 1998-02-17
EP0765395A1 (en) 1997-04-02
AU2733595A (en) 1996-01-15
HUT75840A (en) 1997-05-28
CZ363796A3 (en) 1997-05-14
MX9606536A (es) 1997-03-29
HU9603476D0 (en) 1997-02-28
NO965411D0 (no) 1996-12-16
CN1131312C (zh) 2003-12-17
IL114160A (en) 2006-12-31
FI965030A0 (fi) 1996-12-16
FI965030L (fi) 1997-02-14
WO1995035384A1 (en) 1995-12-28
CA2192943A1 (en) 1995-12-28
FI965030A7 (fi) 1997-02-14
IL114160A0 (en) 1995-10-31
BR9508053A (pt) 1997-08-12
ZA954983B (en) 1996-02-14
PL317761A1 (en) 1997-04-28
CN1152942A (zh) 1997-06-25

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