HUP0203051A2 - Fehérjék előállítása autoproteolitikus hasítással - Google Patents

Fehérjék előállítása autoproteolitikus hasítással

Info

Publication number
HUP0203051A2
HUP0203051A2 HU0203051A HUP0203051A HUP0203051A2 HU P0203051 A2 HUP0203051 A2 HU P0203051A2 HU 0203051 A HU0203051 A HU 0203051A HU P0203051 A HUP0203051 A HU P0203051A HU P0203051 A2 HUP0203051 A2 HU P0203051A2
Authority
HU
Hungary
Prior art keywords
heterologous polypeptide
production
proteins
host cell
fusion protein
Prior art date
Application number
HU0203051A
Other languages
English (en)
Inventor
Franz Knauseder
Tillmann Rümenapf
Heinz-Jürgen Thiel
Jörg Windisch
Original Assignee
Biochemie Gesellschaft M.B.H.,
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biochemie Gesellschaft M.B.H., filed Critical Biochemie Gesellschaft M.B.H.,
Publication of HUP0203051A2 publication Critical patent/HUP0203051A2/hu
Publication of HUP0203051A3 publication Critical patent/HUP0203051A3/hu
Publication of HU230117B1 publication Critical patent/HU230117B1/hu

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

A jelen találmány egy kívánt, egyértelműen definiált homogén N-terminálissal rendelkező heterológ polipeptid előállítási eljárásáravonatkozik egy bakteriális gazdasejtben, amely abban áll, hogy akívánt heterológ polipeptidet autoproteolitikusan hasítják egyelőzetesen expresszált fúziós fehérjéből, amely tartalmaz egypestivírus Npro autoproteáz autoproteolitikus aktivitásával rendelkezőpeptidet és tartalmazza az Npro autoproteolitikus aktivitás általhasítható heterológ polipeptidet. A találmány a fúziós fehérjét kódolónukleinsav-molekulára, ezt tartalmazó vektorra és bakteriálisgazdasejtre is vonatkozik. Ó
HU0203051A 1999-08-09 2000-08-07 Fehérjék előállítása autoproteolitikus hasítással HU230117B1 (hu)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
ATA1368/99 1999-08-09
AT136899 1999-08-09
PCT/EP2000/007642 WO2001011056A1 (en) 1999-08-09 2000-08-07 Production of proteins by autoproteolytic cleavage

Publications (3)

Publication Number Publication Date
HUP0203051A2 true HUP0203051A2 (hu) 2002-12-28
HUP0203051A3 HUP0203051A3 (en) 2003-12-29
HU230117B1 HU230117B1 (hu) 2015-08-28

Family

ID=3512383

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0203051A HU230117B1 (hu) 1999-08-09 2000-08-07 Fehérjék előállítása autoproteolitikus hasítással

Country Status (17)

Country Link
US (1) US7378512B2 (hu)
EP (1) EP1200603B1 (hu)
JP (1) JP5480458B2 (hu)
KR (1) KR100735791B1 (hu)
CN (1) CN1230542C (hu)
AU (1) AU775681B2 (hu)
CA (1) CA2380302C (hu)
CZ (1) CZ303341B6 (hu)
HK (1) HK1047127B (hu)
HU (1) HU230117B1 (hu)
IL (2) IL147411A0 (hu)
MX (1) MXPA02001425A (hu)
NO (1) NO329246B1 (hu)
PL (1) PL203708B1 (hu)
SK (1) SK288290B6 (hu)
TR (1) TR200200048T2 (hu)
WO (1) WO2001011056A1 (hu)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2823220B1 (fr) 2001-04-04 2003-12-12 Genodyssee Nouveaux polynucleotides et polypeptides de l'erythropoietine (epo)
JP2005510244A (ja) * 2001-11-26 2005-04-21 ザ・ユニヴァーシティ・オヴ・クイーンズランド フラビウイルスワクチン送達系
CA2605145A1 (en) 2005-04-26 2006-11-02 Sandoz Ag Affinity ligands
AU2011253661B2 (en) * 2005-04-26 2013-06-13 Boehringer Ingelheim Rcv Gmbh & Co Kg Production of recombinant proteins by autoproteolytic cleavage of a fusion protein
GB0508434D0 (en) * 2005-04-26 2005-06-01 Sandoz Ag Organic compounds
GB0508435D0 (en) * 2005-04-26 2005-06-01 Sandoz Ag Organic compounds
US20100137563A1 (en) 2008-12-03 2010-06-03 Northwestern University Cysteine Protease Autoprocessing of Fusion Proteins
EP2684951A1 (en) 2012-07-13 2014-01-15 Sandoz Ag Method for producing a recombinant protein of interest
EP2746390A1 (en) * 2012-12-19 2014-06-25 Sandoz Ag Method for producing a recombinant protein of interest
EP2746391A1 (en) * 2012-12-19 2014-06-25 Sandoz Ag Method for producing a recombinant protein of interest
UY35874A (es) 2013-12-12 2015-07-31 Novartis Ag Un proceso para la preparación de una composición de proteínas pegiladas

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4743679A (en) * 1986-02-24 1988-05-10 Creative Biomolecules, Inc. Process for producing human epidermal growth factor and analogs thereof
ES2059482T3 (es) 1987-12-23 1994-11-16 Boehringer Ingelheim Int Expresion de la proteasa p2a de hrv2 codificada por virus.
US6077694A (en) * 1990-09-21 2000-06-20 The United States Of America As Represented By The Department Of Health And Human Services Method for over-expression and rapid purification of biosynthetic proteins
AU742794B2 (en) * 1997-04-25 2002-01-10 Sembiosys Genetics Inc. Method for cleavage of fusion proteins
DK1012307T3 (da) * 1997-08-22 2005-09-05 Roche Diagnostics Gmbh Autokatalytisk aktiverbare zymogene proteaseforstadier og deres anvendelse
AU9341398A (en) * 1997-08-22 1999-03-16 Boehringer Mannheim Gmbh Protease precursors that can be autocatalytically activated and their use
HU230231B1 (hu) * 1999-08-09 2015-10-28 Sandoz Ag Fehérjék termelése

Also Published As

Publication number Publication date
EP1200603B1 (en) 2014-12-24
WO2001011056A1 (en) 2001-02-15
KR100735791B1 (ko) 2007-07-06
KR20020021175A (ko) 2002-03-18
CA2380302A1 (en) 2001-02-15
HK1047127B (zh) 2015-08-21
CZ303341B6 (cs) 2012-08-08
EP1200603A1 (en) 2002-05-02
MXPA02001425A (es) 2002-08-12
US20040215008A1 (en) 2004-10-28
AU6699800A (en) 2001-03-05
TR200200048T2 (tr) 2002-04-22
JP2003506092A (ja) 2003-02-18
IL147411A0 (en) 2002-08-14
IL147411A (en) 2013-12-31
HK1047127A1 (en) 2003-02-07
CN1367837A (zh) 2002-09-04
JP5480458B2 (ja) 2014-04-23
HUP0203051A3 (en) 2003-12-29
NO329246B1 (no) 2010-09-20
AU775681B2 (en) 2004-08-12
PL203708B1 (pl) 2009-11-30
CA2380302C (en) 2013-02-26
PL353090A1 (en) 2003-10-06
SK288290B6 (sk) 2015-07-01
CZ2002480A3 (cs) 2002-05-15
NO20020507L (no) 2002-04-05
NO20020507D0 (no) 2002-01-31
US7378512B2 (en) 2008-05-27
SK1872002A3 (en) 2002-07-02
CN1230542C (zh) 2005-12-07
HU230117B1 (hu) 2015-08-28

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