ATE236259T1 - Herstellung einzelner genprodukte eines chimären fusionsproteins durch spaltung mit überall vorkommenden endoproteasen - Google Patents
Herstellung einzelner genprodukte eines chimären fusionsproteins durch spaltung mit überall vorkommenden endoproteasenInfo
- Publication number
- ATE236259T1 ATE236259T1 AT99923745T AT99923745T ATE236259T1 AT E236259 T1 ATE236259 T1 AT E236259T1 AT 99923745 T AT99923745 T AT 99923745T AT 99923745 T AT99923745 T AT 99923745T AT E236259 T1 ATE236259 T1 AT E236259T1
- Authority
- AT
- Austria
- Prior art keywords
- expression
- gene products
- fusion protein
- sequence encoding
- endoproteases
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 5
- 108020001507 fusion proteins Proteins 0.000 title abstract 2
- 229920001184 polypeptide Polymers 0.000 abstract 3
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 3
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 3
- 102000002067 Protein Subunits Human genes 0.000 abstract 2
- 108010001267 Protein Subunits Proteins 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 101710118538 Protease Proteins 0.000 abstract 1
- 238000003776 cleavage reaction Methods 0.000 abstract 1
- 239000000470 constituent Substances 0.000 abstract 1
- 102000037865 fusion proteins Human genes 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 230000007017 scission Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/55—IL-2
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4721—Lipocortins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/5406—IL-4
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/5425—IL-9
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/5434—IL-12
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/515—Animal cells
- A61K2039/5152—Tumor cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Physics & Mathematics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Sorting Of Articles (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9810999.4A GB9810999D0 (en) | 1998-05-21 | 1998-05-21 | Materials and methods relating to the expression of genes |
| PCT/GB1999/001609 WO1999060135A1 (en) | 1998-05-21 | 1999-05-21 | Generation of multiple gene products from chimeric fusion proteins by cleavage with ubiquitous endoproteases |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE236259T1 true ATE236259T1 (de) | 2003-04-15 |
Family
ID=10832516
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT99923745T ATE236259T1 (de) | 1998-05-21 | 1999-05-21 | Herstellung einzelner genprodukte eines chimären fusionsproteins durch spaltung mit überall vorkommenden endoproteasen |
Country Status (13)
| Country | Link |
|---|---|
| EP (1) | EP1080206B1 (de) |
| JP (1) | JP2002515251A (de) |
| KR (1) | KR20010052383A (de) |
| CN (1) | CN1308679A (de) |
| AT (1) | ATE236259T1 (de) |
| AU (1) | AU753785B2 (de) |
| CA (1) | CA2333144A1 (de) |
| DE (1) | DE69906509D1 (de) |
| ES (1) | ES2201718T3 (de) |
| GB (1) | GB9810999D0 (de) |
| ID (1) | ID27206A (de) |
| NZ (1) | NZ508863A (de) |
| WO (1) | WO1999060135A1 (de) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6586411B1 (en) * | 2000-08-16 | 2003-07-01 | Mayo Foundation For Medical Education And Research | System for monitoring the location of transgenes |
| AU7593001A (en) | 2000-07-14 | 2002-01-30 | Zycos Inc | Alpha-msh related compounds and methods of use |
| EP1369128A1 (de) * | 2002-06-07 | 2003-12-10 | Procorde GmbH | Glycoprotein VI Inhibitoren und deren therapeutische Verwendung |
| US7423123B2 (en) * | 2003-05-14 | 2008-09-09 | Aerovance, Inc. | Interleukin-9 antagonist muteins and their pharmacological methods of use |
| US7485291B2 (en) * | 2003-06-03 | 2009-02-03 | Cell Genesys, Inc. | Compositions and methods for generating multiple polypeptides from a single vector using a virus derived peptide cleavage site, and uses thereof |
| EP1636360A4 (de) * | 2003-06-03 | 2006-11-08 | Cell Genesys Inc | Zusammensetzungen und verfahren zur verstärkten expression rekombinanter polypeptide von einem vektor unter verwendung einer peptidspaltstelle |
| FR2856069A1 (fr) * | 2003-06-10 | 2004-12-17 | Bionexis | Molecules de ciblage et de liberation de composes therapeutiques et leur utilisation |
| EP2468881A3 (de) | 2005-07-21 | 2012-08-15 | Abbott Laboratories | Mehrfache Genexpression mit sORF-Konstrukten und Verfahren mit Polyproteinen, Pro-Proteinen und Proteolyse |
| US8871906B2 (en) * | 2007-09-04 | 2014-10-28 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Deletions in domain II of pseudomonas exotoxin a that remove immunogenic epitopes |
| US8715964B2 (en) | 2008-05-11 | 2014-05-06 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Expression of IL-12 family heterodimers |
| WO2011042874A1 (en) * | 2009-10-06 | 2011-04-14 | Ecole Polytechnique Federale De Lausanne (Epfl) | Visualization of proprotein convertase activity in living cells and tissues |
| US9951117B2 (en) | 2010-09-02 | 2018-04-24 | Mayo Foundation For Medical Education And Research | Vesicular stomatitis viruses |
| CA2847413C (en) | 2010-09-02 | 2018-05-22 | Mayo Foundation For Medical Education And Research | Vesicular stomatitis viruses |
| EP3197911A4 (de) * | 2014-09-22 | 2018-06-20 | Intrexon Corporation | Verbesserter therapeutische kontrolle von heterodimeren und einkettigen formen von interleukin-12 |
| AU2016334401A1 (en) * | 2015-10-10 | 2018-04-26 | Intrexon Corporation | Improved therapeutic control of proteolytically sensitive, destabilized forms of interleukin-12 |
| BR112020015030A2 (pt) * | 2018-09-17 | 2021-03-16 | Gi Innovation, Inc. | Proteína de fusão compreendendo a proteína il-2 e a proteína cd80 e uso da mesma |
| EP3941531A4 (de) * | 2019-03-22 | 2023-01-18 | The Johns Hopkins University | Genfreisetzungspartikel zur induzierung von antigen-präsentierenden zellen aus tumoren |
| WO2022212896A1 (en) * | 2021-04-02 | 2022-10-06 | Krystal Biotech, Inc. | Viral vectors for cancer therapy |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2000859A6 (es) * | 1985-08-12 | 1988-03-16 | Syntex Inc | Un metodo para producir un vector de expresion bacteriana para la expresion de proteinas de fusion |
| ATE97958T1 (de) * | 1989-08-11 | 1993-12-15 | Zymogenetics Inc | Zellenzuchtverfahren fuer die produktion von aktiviertem protein-c. |
| JPH11513249A (ja) * | 1995-09-27 | 1999-11-16 | メディカル・リサーチ・カウンシル | プロテアーゼにより開裂可能なタンパク質を組込んだ組換えウイルス |
-
1998
- 1998-05-21 GB GBGB9810999.4A patent/GB9810999D0/en not_active Ceased
-
1999
- 1999-05-21 DE DE69906509T patent/DE69906509D1/de not_active Expired - Lifetime
- 1999-05-21 WO PCT/GB1999/001609 patent/WO1999060135A1/en not_active Application Discontinuation
- 1999-05-21 AU AU40508/99A patent/AU753785B2/en not_active Ceased
- 1999-05-21 AT AT99923745T patent/ATE236259T1/de not_active IP Right Cessation
- 1999-05-21 CA CA002333144A patent/CA2333144A1/en not_active Abandoned
- 1999-05-21 ID IDW20002666A patent/ID27206A/id unknown
- 1999-05-21 EP EP99923745A patent/EP1080206B1/de not_active Expired - Lifetime
- 1999-05-21 CN CN99808292A patent/CN1308679A/zh active Pending
- 1999-05-21 KR KR1020007013081A patent/KR20010052383A/ko not_active Application Discontinuation
- 1999-05-21 NZ NZ508863A patent/NZ508863A/xx unknown
- 1999-05-21 JP JP2000549741A patent/JP2002515251A/ja active Pending
- 1999-05-21 ES ES99923745T patent/ES2201718T3/es not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CN1308679A (zh) | 2001-08-15 |
| CA2333144A1 (en) | 1999-11-25 |
| DE69906509D1 (de) | 2003-05-08 |
| JP2002515251A (ja) | 2002-05-28 |
| GB9810999D0 (en) | 1998-07-22 |
| EP1080206A1 (de) | 2001-03-07 |
| KR20010052383A (ko) | 2001-06-25 |
| WO1999060135A8 (en) | 2000-01-13 |
| NZ508863A (en) | 2003-03-28 |
| AU4050899A (en) | 1999-12-06 |
| AU753785B2 (en) | 2002-10-31 |
| EP1080206B1 (de) | 2003-04-02 |
| ES2201718T3 (es) | 2004-03-16 |
| ID27206A (id) | 2001-03-08 |
| WO1999060135A1 (en) | 1999-11-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |