DK1625219T3 - Fremgangsmåder til fremstilling af secernerede polypeptider - Google Patents

Fremgangsmåder til fremstilling af secernerede polypeptider

Info

Publication number
DK1625219T3
DK1625219T3 DK04751203.3T DK04751203T DK1625219T3 DK 1625219 T3 DK1625219 T3 DK 1625219T3 DK 04751203 T DK04751203 T DK 04751203T DK 1625219 T3 DK1625219 T3 DK 1625219T3
Authority
DK
Denmark
Prior art keywords
nucleotide sequence
polypeptide
signal peptide
methods
fungal host
Prior art date
Application number
DK04751203.3T
Other languages
English (en)
Inventor
Suchindra Maiyuran
Ana Fidantsef
Howard Brody
Original Assignee
Novozymes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Inc filed Critical Novozymes Inc
Application granted granted Critical
Publication of DK1625219T3 publication Critical patent/DK1625219T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DK04751203.3T 2003-05-02 2004-04-30 Fremgangsmåder til fremstilling af secernerede polypeptider DK1625219T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46776603P 2003-05-02 2003-05-02
PCT/US2004/013711 WO2004111228A1 (en) 2003-05-02 2004-04-30 Methods for producing secreted polypeptides

Publications (1)

Publication Number Publication Date
DK1625219T3 true DK1625219T3 (da) 2010-11-01

Family

ID=33551405

Family Applications (1)

Application Number Title Priority Date Filing Date
DK04751203.3T DK1625219T3 (da) 2003-05-02 2004-04-30 Fremgangsmåder til fremstilling af secernerede polypeptider

Country Status (8)

Country Link
US (4) US7393664B2 (da)
EP (1) EP1625219B1 (da)
CN (1) CN1836038B (da)
AT (1) ATE478140T1 (da)
DE (1) DE602004028724D1 (da)
DK (1) DK1625219T3 (da)
ES (1) ES2350903T3 (da)
WO (1) WO2004111228A1 (da)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1902310B (zh) * 2003-10-28 2011-09-21 诺维信股份有限公司 具有β-葡糖苷酶活性的多肽和编码所述多肽的多核苷酸
WO2005100582A2 (en) 2004-03-25 2005-10-27 Novozymes Inc. Methods for degrading or converting plant cell wall polysaccharides
MX2007014229A (es) * 2005-05-19 2008-02-07 Novozymes North America Inc Produccion de enzimas.
EP2032703B1 (en) * 2006-06-26 2011-03-23 DSM IP Assets B.V. High throughput transfection of filamentous fungi
US8546106B2 (en) * 2006-07-21 2013-10-01 Novozymes, Inc. Methods of increasing secretion of polypeptides having biological activity
DK2195424T3 (da) * 2007-09-28 2019-01-07 Novozymes As Polypeptider med cellobiohydrolase II-aktivitet og polynukleotider, som koder for dem
US9012186B2 (en) 2009-04-27 2015-04-21 The Board Of Trustees Of The University Of Illinois Hemicellulose-degrading enzymes
EP2397491A1 (en) 2010-06-21 2011-12-21 Technische Universität Wien LeaA from Trichoderma reesei
WO2012060389A1 (ja) * 2010-11-05 2012-05-10 旭硝子株式会社 シゾサッカロミセス属酵母の形質転換体およびその製造方法
BR112014004186B1 (pt) 2011-08-24 2020-12-15 Novozymes, Inc. Composição enzimática, célula hospedeira de fungo filamentoso recombinante, método para produzir uma composição enzimática, processos para degradar um material celulósico, para sintetizar um produto de fermentação, e, para fermentar um material celulósico
CN103184159B (zh) * 2011-12-27 2016-09-21 丰益(上海)生物技术研发中心有限公司 紧密枝顶孢霉菌株及其特异性脂肪酶
EP2708553A1 (en) 2012-09-18 2014-03-19 Technische Universität Wien Modified fungal cell
CN105229141A (zh) 2013-03-15 2016-01-06 拉勒曼德匈牙利流动管理有限责任公司 用于木质纤维素和相关低聚物的水解的β-葡糖苷酶的表达
EP3074513A1 (en) 2013-11-26 2016-10-05 Novozymes A/S Enzyme compositions and uses thereof
WO2015187935A1 (en) 2014-06-06 2015-12-10 Novozymes A/S Enzyme compositions and uses thereof
CN107429255B (zh) 2015-03-09 2022-01-25 诺维信公司 将多种表达构建体引入真核细胞的方法
CN104975039B (zh) * 2015-06-24 2018-05-11 方诩 一种重组质粒及其在降解纤维素原料中的应用
US11268118B2 (en) 2015-10-30 2022-03-08 Suntory Holdings Limited Method for producing steviol and steviol glycoside using AOBGL1 homolog
EP3369823A4 (en) * 2015-10-30 2019-04-03 Suntory Holdings Limited PROCESS FOR PRODUCING STEVIOL AND STEVIOL GLYCOSIDE USING AOBGL3 APPROVAL
EP3541954A1 (en) 2016-11-21 2019-09-25 Novozymes A/S Yeast cell extract assisted construction of dna molecules
CN110114455A (zh) * 2016-12-27 2019-08-09 国立大学法人鹿儿岛大学 真菌中蛋白质的选择性分泌技术
EP3884027A1 (en) * 2018-11-19 2021-09-29 Basf Se Leader sequence for yeast
CN112048468B (zh) * 2019-09-03 2021-04-06 南京市妇幼保健院 一种卵母细胞体外成熟培养试剂
CN111378674B (zh) * 2020-03-25 2021-09-10 中国科学院天津工业生物技术研究所 异宗毁丝霉糖化酶MhglaA及其编码基因和在葡萄糖生产中的应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4220721A (en) * 1979-04-27 1980-09-02 University Of Arkansas Foundation Method for enzyme reutilization
DK187280A (da) * 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
WO1991017243A1 (en) * 1990-05-09 1991-11-14 Novo Nordisk A/S A cellulase preparation comprising an endoglucanase enzyme
US5861271A (en) * 1993-12-17 1999-01-19 Fowler; Timothy Cellulase enzymes and systems for their expressions
DE69736606T2 (de) * 1996-07-24 2007-09-20 Meiji Seika K.K. Cellulase und Cellulase-Präperation, die dieselbe enthält
US6015703A (en) * 1998-03-10 2000-01-18 Iogen Corporation Genetic constructs and genetically modified microbes for enhanced production of beta-glucosidase

Also Published As

Publication number Publication date
US20080268526A1 (en) 2008-10-30
US20040248258A1 (en) 2004-12-09
WO2004111228A1 (en) 2004-12-23
US20140011262A1 (en) 2014-01-09
EP1625219A1 (en) 2006-02-15
ES2350903T3 (es) 2011-01-28
CN1836038A (zh) 2006-09-20
EP1625219B1 (en) 2010-08-18
US20120122191A1 (en) 2012-05-17
US7393664B2 (en) 2008-07-01
ATE478140T1 (de) 2010-09-15
US8497115B2 (en) 2013-07-30
CN1836038B (zh) 2011-04-06
DE602004028724D1 (de) 2010-09-30
EP1625219A4 (en) 2007-09-05

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