DE69426289D1 - Signalsequenzen für die Secretion heterologer Proteine von Hefe - Google Patents

Signalsequenzen für die Secretion heterologer Proteine von Hefe

Info

Publication number
DE69426289D1
DE69426289D1 DE69426289T DE69426289T DE69426289D1 DE 69426289 D1 DE69426289 D1 DE 69426289D1 DE 69426289 T DE69426289 T DE 69426289T DE 69426289 T DE69426289 T DE 69426289T DE 69426289 D1 DE69426289 D1 DE 69426289D1
Authority
DE
Germany
Prior art keywords
secretion
heterologous proteins
yeast
signal sequences
secretion signal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69426289T
Other languages
English (en)
Other versions
DE69426289T2 (de
Inventor
Bong Hyun Chung
Soo Wan Nam
Byung Moon Kim
Sun Ah Yang
Young Hoon Park
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Korea Institute of Science and Technology KIST
Original Assignee
Korea Institute of Science and Technology KIST
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Korea Institute of Science and Technology KIST filed Critical Korea Institute of Science and Technology KIST
Publication of DE69426289D1 publication Critical patent/DE69426289D1/de
Application granted granted Critical
Publication of DE69426289T2 publication Critical patent/DE69426289T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4721—Lipocortins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01007—Inulinase (3.2.1.7)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE69426289T 1993-12-10 1994-12-08 Signalsequenzen für die Secretion heterologer Proteine von Hefe Expired - Fee Related DE69426289T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR930027269 1993-12-10

Publications (2)

Publication Number Publication Date
DE69426289D1 true DE69426289D1 (de) 2000-12-21
DE69426289T2 DE69426289T2 (de) 2001-04-12

Family

ID=19370595

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69426289T Expired - Fee Related DE69426289T2 (de) 1993-12-10 1994-12-08 Signalsequenzen für die Secretion heterologer Proteine von Hefe

Country Status (5)

Country Link
US (1) US5712113A (de)
EP (1) EP0662515B1 (de)
JP (1) JP2799348B2 (de)
AT (1) ATE197608T1 (de)
DE (1) DE69426289T2 (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0773296B1 (de) * 1995-02-03 2006-09-13 Asahi Glass Company Ltd. Gen für ein sekretionssignal und dieses enthaltender vektor
MXPA04009553A (es) * 2002-04-22 2005-01-25 Procter & Gamble Champu que contiene un derivado de guar cationico.
EP1633317A4 (de) 2003-05-16 2008-08-20 Intermune Inc Synthetische chemokin-rezeptor-liganden und anwendungsverfahren dafür
DE10342794A1 (de) * 2003-09-16 2005-04-21 Basf Ag Sekretion von Proteinen aus Hefen
EP2275556B1 (de) * 2004-01-17 2013-04-24 Korea Research Institute of Bioscience and Biotechnology Schnell-Screeningverfahren für Translationsfusionspartner zur Herstellung rekombinanter Proteine und daraus gescreente Translationsfusionspartner
US7597884B2 (en) * 2004-08-09 2009-10-06 Alios Biopharma, Inc. Hyperglycosylated polypeptide variants and methods of use
JP2008513356A (ja) * 2004-08-09 2008-05-01 アリオス バイオファーマ インク． 合成高度糖鎖付加プロテアーゼ耐性ポリペプチド変異体、それを使用する経口製剤および方法
CA2596661C (en) 2005-02-07 2013-12-10 Basf Aktiengesellschaft Novel hydrophobin fusion products, production and use thereof
US8859106B2 (en) 2005-03-31 2014-10-14 Basf Se Use of polypeptides in the form of adhesive agents
DK1869138T3 (da) 2005-04-01 2010-03-01 Basf Se Borevæske indeholdende hydrophobin
EP1868700A2 (de) 2005-04-01 2007-12-26 Basf Aktiengesellschaft Verwendung von hydrophobin als phasen-stabilisator
DE102005027139A1 (de) 2005-06-10 2006-12-28 Basf Ag Neue Cystein-verarmte Hydrophobinfusionsproteine, deren Herstellung und Verwendung
DE102005048720A1 (de) 2005-10-12 2007-04-19 Basf Ag Verwendung von Proteinen als Antischaum-Komponente in Kraftstoffen
JP5354559B2 (ja) * 2005-11-24 2013-11-27 独立行政法人産業技術総合研究所 高効率分泌シグナルペプチド及びそれらを利用したタンパク質発現系
ES2374320T3 (es) 2006-08-15 2012-02-15 Basf Se Procedimiento para la producción de preparaciones de hidrofobina secas de flujo libre.
JP5769142B2 (ja) * 2007-09-04 2015-08-26 独立行政法人酒類総合研究所 ピヒア属酵母において異種タンパク質を高分泌させる方法
WO2010064748A1 (en) * 2008-12-04 2010-06-10 Korea Research Institute Of Bioscience And Biotechnology Screening of abundantly secreted proteins and their use as fusion partners for the production of recombinant proteins
KR101291241B1 (ko) 2010-11-04 2013-08-01 한국생명공학연구원 효모에서 인체 상피세포 성장인자를 대량 생산하는 방법

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4588684A (en) * 1983-04-26 1986-05-13 Chiron Corporation a-Factor and its processing signals
US5081019A (en) * 1985-01-10 1992-01-14 Biogen, Inc. DNA sequences, recombinant DNA molecules and processes for producing lipocortin-like polypeptides
IT1196484B (it) * 1986-07-11 1988-11-16 Sclavo Spa Vettore ad espressione e secrezione in lieviti,utile per la preparazione di proteine eterologhe
WO1991005051A1 (en) * 1989-09-28 1991-04-18 Leningradsky Gosudarstvenny Universitet Method of obtaining human-interleukin-2 polypeptide in yeast cells
WO1994013821A1 (en) * 1992-12-11 1994-06-23 Quest International B.V. The use of the kluyveromyces marxianus inulinase gene promoter for protein production

Also Published As

Publication number Publication date
JP2799348B2 (ja) 1998-09-17
ATE197608T1 (de) 2000-12-15
JPH0847393A (ja) 1996-02-20
EP0662515A1 (de) 1995-07-12
DE69426289T2 (de) 2001-04-12
EP0662515B1 (de) 2000-11-15
US5712113A (en) 1998-01-27

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