KR950032626A - 천연 n-말단 서열을 갖는 재조합 아프로티닌 및 재조합 아프로티닌 변형체의 제조 방법 - Google Patents
천연 n-말단 서열을 갖는 재조합 아프로티닌 및 재조합 아프로티닌 변형체의 제조 방법 Download PDFInfo
- Publication number
- KR950032626A KR950032626A KR1019950012209A KR19950012209A KR950032626A KR 950032626 A KR950032626 A KR 950032626A KR 1019950012209 A KR1019950012209 A KR 1019950012209A KR 19950012209 A KR19950012209 A KR 19950012209A KR 950032626 A KR950032626 A KR 950032626A
- Authority
- KR
- South Korea
- Prior art keywords
- aprotinin
- recombinant aprotinin
- native
- methods
- variants
- Prior art date
Links
- 108010039627 Aprotinin Proteins 0.000 title claims abstract 10
- 229960004405 aprotinin Drugs 0.000 title claims abstract 10
- ZPNFWUPYTFPOJU-LPYSRVMUSA-N iniprol Chemical compound C([C@H]1C(=O)NCC(=O)NCC(=O)N[C@H]2CSSC[C@H]3C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@H](C(N[C@H](C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC=4C=CC=CC=4)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC=4C=CC=CC=4)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC2=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC=2C=CC=CC=2)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H]2N(CCC2)C(=O)[C@@H](N)CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N2[C@@H](CCC2)C(=O)N2[C@@H](CCC2)C(=O)N[C@@H](CC=2C=CC(O)=CC=2)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N2[C@@H](CCC2)C(=O)N3)C(=O)NCC(=O)NCC(=O)N[C@@H](C)C(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@H](C(=O)N1)C(C)C)[C@@H](C)O)[C@@H](C)CC)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 ZPNFWUPYTFPOJU-LPYSRVMUSA-N 0.000 title claims abstract 10
- 108090000623 proteins and genes Proteins 0.000 claims abstract 3
- ZPNFWUPYTFPOJU-MPSLMFKFSA-N aprotinin Chemical compound CC[C@H](C)[C@@H]1NC(=O)[C@@H](CCCNC(N)=N)NC(=O)[C@@H](C)NC(=O)[C@H](CCCCN)NC(=O)[C@@H]2CSSC[C@H]3NC(=O)CNC(=O)CNC(=O)[C@H](Cc4ccc(O)cc4)NC(=O)[C@H](NC(=O)[C@H](Cc4ccccc4)NC(=O)[C@@H](NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](CSSC[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](Cc4ccccc4)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](CC(N)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC3=O)C(=O)N[C@H](CCSC)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@H](CSSC[C@@H](NC(=O)[C@H](Cc3ccccc3)NC(=O)[C@@H](CC(O)=O)NC(=O)[C@H]3CCCN3C(=O)[C@H](N)CCCNC(N)=N)C(=O)N[C@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N3CCC[C@@H]3C(=O)N3CCC[C@H]3C(=O)N[C@H](Cc3ccc(O)cc3)C(=O)N[C@H]([C@H](C)O)C(=O)NCC(=O)N3CCC[C@H]3C(=O)N2)C(=O)NCC(=O)NCC(=O)N[C@H](C)C(O)=O)NC(=O)[C@@H](CC(C)C)NC(=O)CNC(=O)[C@@H](C)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](C)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](Cc2ccc(O)cc2)NC(=O)[C@H](Cc2ccccc2)NC(=O)[C@@H](Cc2ccc(O)cc2)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](NC1=O)[C@H](C)CC)[C@@H](C)O)C(C)C ZPNFWUPYTFPOJU-MPSLMFKFSA-N 0.000 claims 2
- 244000005700 microbiome Species 0.000 claims 2
- 239000013598 vector Substances 0.000 claims 2
- 101150023810 PHO1 gene Proteins 0.000 claims 1
- 101150012394 PHO5 gene Proteins 0.000 claims 1
- 108010076504 Protein Sorting Signals Proteins 0.000 claims 1
- 101100271429 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) ATP6 gene Proteins 0.000 claims 1
- 101100115751 Trypanosoma brucei brucei dnaaf11 gene Proteins 0.000 claims 1
- IXKSXJFAGXLQOQ-XISFHERQSA-N WHWLQLKPGQPMY Chemical compound C([C@@H](C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(O)=O)NC(=O)[C@@H](N)CC=1C2=CC=CC=C2NC=1)C1=CNC=N1 IXKSXJFAGXLQOQ-XISFHERQSA-N 0.000 claims 1
- 108010051210 beta-Fructofuranosidase Proteins 0.000 claims 1
- 239000012228 culture supernatant Substances 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 239000013604 expression vector Substances 0.000 claims 1
- 235000011073 invertase Nutrition 0.000 claims 1
- 239000001573 invertase Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 108010087967 type I signal peptidase Proteins 0.000 claims 1
- 238000011144 upstream manufacturing Methods 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
- C07K14/8114—Kunitz type inhibitors
- C07K14/8117—Bovine/basic pancreatic trypsin inhibitor (BPTI, aprotinin)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
본 발명은 천연 N-말단 서열을 갖는 재조합 아프로티닌 및 재조합 아프로티닌 변형체의 제조 방법에 관한 것으로 재조합 아프로티닌 및 (또는) 재조합 아프로티닌 변형체는 균질하게 프로세싱된, 분비된 단백질로서 존재한다.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
Claims (3)
- a) 아프로티닌 또는 그 변형체를 위한 유전자, 및 b) 이 유전자의 직접 상류의 리더로서, 신호 펩티다제에 의해 프로세싱되는 프리 서열을 위한 뉴클레오티드 서열을 함유하는, 정확한 N-말단을 갖는 아프로티닌 및 아프로티닌 변형체의 발현 벡터.
- 제1항에 있어서, 리더 신호 서열이 프리 알파 인자, PHO1, PHO5 또는 인버타제로부터 유래되는 벡터.
- 미생물이 제1 또는 2항에 따른 벡터를 함유하는 것을 특징으로 하는, 미생물을 배양하고 배양 상등액으로부터 아프로티닌 또는 그 변형체를 분리함으로써 정확한 N-말단을 갖는 아프로티닌 및 아프로티닌 변형체를 제조하는 방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE4417353A DE4417353A1 (de) | 1994-05-18 | 1994-05-18 | Verfahren zur Herstellung von rekombinantem Aprotinin und rekombinanten Aprotinin Varianten mit der natürlichen N-terminlaen Sequenz |
| DEP4417353.9 | 1994-05-18 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR950032626A true KR950032626A (ko) | 1995-12-22 |
Family
ID=6518348
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019950012209A KR950032626A (ko) | 1994-05-18 | 1995-05-17 | 천연 n-말단 서열을 갖는 재조합 아프로티닌 및 재조합 아프로티닌 변형체의 제조 방법 |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US5707831A (ko) |
| EP (1) | EP0683229A3 (ko) |
| JP (1) | JPH08163988A (ko) |
| KR (1) | KR950032626A (ko) |
| CN (1) | CN1123325A (ko) |
| AU (1) | AU705267B2 (ko) |
| CA (1) | CA2149375A1 (ko) |
| DE (1) | DE4417353A1 (ko) |
| FI (1) | FI952372A (ko) |
| IL (1) | IL113725A0 (ko) |
| NO (1) | NO951926L (ko) |
| NZ (1) | NZ272121A (ko) |
| ZA (1) | ZA954005B (ko) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100838915B1 (ko) * | 2003-12-18 | 2008-06-16 | 캐리어 코포레이션 | Ｈｖａｃ 시스템 내에서의 폐색된 필터의 검출 |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19952622A1 (de) * | 1999-11-02 | 2001-05-10 | Bayer Ag | Verfahren zur Herstellung von rekombinantem Interleukin-8 und Interleukin-8 Muteinen |
| US20060218667A1 (en) * | 2004-10-12 | 2006-09-28 | Vojdani Fakhrieh S | Plant-produced recombinant aprotinin and aprotinin variants |
| WO2008110301A1 (de) * | 2007-03-13 | 2008-09-18 | Bayer Schering Pharma Aktiengesellschaft | Aprotinin-varianten mit verbesserten eigenschaften |
| WO2008139591A1 (ja) * | 2007-05-11 | 2008-11-20 | Juridical Foundation The Chemo-Sero-Therapeutic Research Institute | 組換えアプロチニンの生産方法 |
| CN101538318B (zh) * | 2009-03-06 | 2011-11-23 | 中国人民解放军军事医学科学院生物工程研究所 | 一种信号肽及其编码基因与应用 |
| CN107998375B (zh) * | 2017-12-14 | 2021-11-05 | 广东医科大学 | 一种多肽在预防或治疗血栓性疾病或脑缺血再灌注损伤相关疾病中的应用 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU560584B2 (en) * | 1983-07-28 | 1987-04-09 | Bayer Aktiengesellschaft | Homologues of aprotinin |
| GB2208511A (en) * | 1987-08-07 | 1989-04-05 | Bayer Ag | Variants of bovine pancreatic trypsin inhibitor produced by recombinant dna technology |
| DK450187D0 (da) * | 1987-08-28 | 1987-08-28 | Novo Industri As | Fremgangsmaade til fremstilling af proteiner |
| US5591603A (en) * | 1987-08-28 | 1997-01-07 | Novo Nordisk A/S | Process for preparing aprotinin and aprotinin analogs in yeast cells |
| DK463887D0 (da) * | 1987-09-07 | 1987-09-07 | Novo Industri As | Gaerleader |
| DK105489D0 (da) * | 1989-03-03 | 1989-03-03 | Novo Nordisk As | Polypeptid |
| DE3930522A1 (de) * | 1989-09-13 | 1991-03-21 | Bayer Ag | Rekombinante aprotinin-varianten - gentechnisches verfahren zur mikrobiellen herstellung von homogen prozessierten aprotinin-varianten sowie die therapeutische anwendung derselben |
| US5258302A (en) * | 1990-07-03 | 1993-11-02 | The Salk Institute Biotechnology/Industrial Associates, Inc. | DNA for expression of aprotinin in methylotrophic yeast cells |
-
1994
- 1994-05-18 DE DE4417353A patent/DE4417353A1/de not_active Withdrawn
-
1995
- 1995-05-05 EP EP95106801A patent/EP0683229A3/de not_active Withdrawn
- 1995-05-11 US US08/439,585 patent/US5707831A/en not_active Expired - Fee Related
- 1995-05-12 AU AU20045/95A patent/AU705267B2/en not_active Ceased
- 1995-05-15 IL IL11372595A patent/IL113725A0/xx unknown
- 1995-05-15 NZ NZ272121A patent/NZ272121A/en unknown
- 1995-05-15 CN CN95106042A patent/CN1123325A/zh active Pending
- 1995-05-15 CA CA002149375A patent/CA2149375A1/en not_active Abandoned
- 1995-05-16 JP JP7141306A patent/JPH08163988A/ja active Pending
- 1995-05-16 NO NO951926A patent/NO951926L/no unknown
- 1995-05-16 FI FI952372A patent/FI952372A/fi unknown
- 1995-05-17 KR KR1019950012209A patent/KR950032626A/ko not_active Application Discontinuation
- 1995-05-17 ZA ZA954005A patent/ZA954005B/xx unknown
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100838915B1 (ko) * | 2003-12-18 | 2008-06-16 | 캐리어 코포레이션 | Ｈｖａｃ 시스템 내에서의 폐색된 필터의 검출 |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2004595A (en) | 1995-11-23 |
| DE4417353A1 (de) | 1996-01-25 |
| IL113725A0 (en) | 1995-08-31 |
| EP0683229A3 (de) | 1996-01-17 |
| NO951926L (no) | 1995-11-20 |
| FI952372A0 (fi) | 1995-05-16 |
| EP0683229A2 (de) | 1995-11-22 |
| US5707831A (en) | 1998-01-13 |
| NZ272121A (en) | 1996-01-26 |
| NO951926D0 (no) | 1995-05-16 |
| AU705267B2 (en) | 1999-05-20 |
| ZA954005B (en) | 1996-01-17 |
| FI952372A (fi) | 1995-11-19 |
| JPH08163988A (ja) | 1996-06-25 |
| CA2149375A1 (en) | 1995-11-19 |
| CN1123325A (zh) | 1996-05-29 |
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| WITN | Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid |