DE69227116D1 - Hochstabile rekombinante hefen für die herstellung von rekombinanten proteinen - Google Patents

Hochstabile rekombinante hefen für die herstellung von rekombinanten proteinen

Info

Publication number
DE69227116D1
DE69227116D1 DE69227116T DE69227116T DE69227116D1 DE 69227116 D1 DE69227116 D1 DE 69227116D1 DE 69227116 T DE69227116 T DE 69227116T DE 69227116 T DE69227116 T DE 69227116T DE 69227116 D1 DE69227116 D1 DE 69227116D1
Authority
DE
Germany
Prior art keywords
recombinant
highly stable
years
production
proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69227116T
Other languages
English (en)
Other versions
DE69227116T2 (de
Inventor
Reinhard Fleer
Alain Fournier
Patrice Yeh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Aventis Pharma SA
Original Assignee
Rhone Poulenc Rorer SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rhone Poulenc Rorer SA filed Critical Rhone Poulenc Rorer SA
Publication of DE69227116D1 publication Critical patent/DE69227116D1/de
Application granted granted Critical
Publication of DE69227116T2 publication Critical patent/DE69227116T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DE69227116T 1991-08-02 1992-08-03 Hochstabile rekombinante hefen für die herstellung von rekombinanten proteinen Expired - Lifetime DE69227116T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9109854A FR2679920A1 (fr) 1991-08-02 1991-08-02 Levures recombinantes hautement stables pour la production de proteines recombinantes, leur preparation et leur utilisation.
PCT/FR1992/000769 WO1993003159A1 (fr) 1991-08-02 1992-08-03 Levures recombinantes hautement stables pour la production de proteines recombinantes

Publications (2)

Publication Number Publication Date
DE69227116D1 true DE69227116D1 (de) 1998-10-29
DE69227116T2 DE69227116T2 (de) 1999-03-25

Family

ID=9415866

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69227116T Expired - Lifetime DE69227116T2 (de) 1991-08-02 1992-08-03 Hochstabile rekombinante hefen für die herstellung von rekombinanten proteinen

Country Status (13)

Country Link
US (2) US5593858A (de)
EP (2) EP0597035B1 (de)
JP (1) JPH06509234A (de)
AT (1) ATE171474T1 (de)
AU (1) AU2474892A (de)
CA (1) CA2113551A1 (de)
DE (1) DE69227116T2 (de)
DK (1) DK0597035T3 (de)
ES (1) ES2123006T3 (de)
FR (1) FR2679920A1 (de)
NZ (1) NZ243782A (de)
WO (1) WO1993003159A1 (de)
ZA (1) ZA925735B (de)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6265186B1 (en) 1997-04-11 2001-07-24 Dsm N.V. Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces
WO2000051623A2 (en) 1999-03-05 2000-09-08 The Trustees Of University Technology Corporation Inhibitors of serine protease activity, methods and compositions for treatment of nitric oxide-induced clinical conditions
US6849605B1 (en) * 1999-03-05 2005-02-01 The Trustees Of University Technology Corporation Inhibitors of serine protease activity, methods and compositions for treatment of viral infections
AU3731400A (en) * 1999-03-05 2000-09-21 Trustees Of University Technology Corporation, The Methods and compositions useful in inhibiting apoptosis
DE10101962B4 (de) * 2001-01-17 2006-07-27 Tad Pharma Gmbh DNA-Sequenz mit regulatorischen Bereichen zur Expression von Proteinen
WO2004050683A2 (en) * 2002-12-02 2004-06-17 Abgenix, Inc. Antibodies directed to tumor necrosis factor and uses thereof
US20040220242A1 (en) * 2003-05-02 2004-11-04 Leland Shapiro Inhibitors of serine protease activity, methods and compositions for treatment of nitric oxide induced clinical conditions
DE102008057451A1 (de) * 2008-11-14 2010-05-20 Martin-Luther-Universität Halle-Wittenberg Verfahren zur oralen Vakzinierung mittels rekombinanter Hefen
US9334488B2 (en) 2008-12-24 2016-05-10 Dsm Ip Assets B.V. Xylose isomerase genes and their use in fermentation of pentose sugars
UA108853C2 (uk) 2009-07-10 2015-06-25 Спосіб ферментації галактози
PL2561064T3 (pl) 2010-04-21 2018-03-30 Dsm Ip Assets B.V. Komórka odpowiednia do fermentacji kompozycji mieszanych cukrów
WO2011149353A1 (en) 2010-05-27 2011-12-01 C5 Yeast Company B.V. Yeast strains engineered to produce ethanol from acetic acid and glycerol
EP2576804B1 (de) 2010-05-28 2016-12-14 Codexis, Inc. Pentosefermentierung durch einen rekombinanten mikroorganismus
JP5961886B2 (ja) 2010-10-13 2016-08-03 ディーエスエム アイピー アセッツ ビー．ブイ． ペントースおよびグルコース発酵酵母細胞
ES2532508T3 (es) 2010-11-18 2015-03-27 Dsm Ip Assets B.V. Cepas de levaduras manipuladas para producir etanol a partir de glicerol
WO2012125027A1 (en) 2011-03-14 2012-09-20 Dsm Ip Assets B.V. Yeast strains that ferment uronic acids
WO2012135110A1 (en) 2011-03-30 2012-10-04 Codexis, Inc. Pentose fermentation by a recombinant microorganism
AR086471A1 (es) 2011-04-22 2013-12-18 Dsm Ip Assets Bv Celula de levadura capaz de convertir azucares que incluyen arabinosa y xilosa
US8778652B2 (en) 2011-06-30 2014-07-15 Codexis, Inc. Pentose fermentation by a recombinant microorganism
EP2546336A1 (de) 2011-07-11 2013-01-16 DSM IP Assets B.V. Hefestränge, die Uronsäure verbrauchen und Fermentierungsprodukte wie Ethanol herstellen
EP2554668A1 (de) 2011-08-04 2013-02-06 DSM IP Assets B.V. Zelle zur Fermentierung von Pentosezucker
AR087423A1 (es) 2011-08-04 2014-03-19 Dsm Ip Assets Bv Celula capaz de fermentar azucares pentosas
EP2785849B1 (de) 2011-11-30 2017-09-27 DSM IP Assets B.V. Manipulierte hefestämme zur herstellung von ethanol aus acetatsäure und glycerol
DK3327112T3 (en) 2012-07-19 2020-05-04 Dsm Ip Assets Bv Agse-deficient stamme
IN2015DN01041A (de) 2012-08-28 2015-06-26 Dsm Ip Assets Bv
WO2014033019A1 (en) 2012-08-28 2014-03-06 Dsm Ip Assets B.V. Yeast strains engineered to produce ethanol from acetate
ES2717682T3 (es) 2012-10-16 2019-06-24 Dsm Ip Assets Bv Células con conversión mejorada de pentosas
EP2922953A4 (de) 2012-11-20 2016-11-09 Shell Int Research Pentosefermentierung durch einen rekombinanten mikroorganismus
MY176331A (en) 2013-02-04 2020-07-29 Dsm Ip Assets Bv Carbohydrate degrading polypeptide and uses thereof
WO2014202624A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202620A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202622A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202621A1 (en) 2013-06-20 2014-12-24 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
WO2014207113A1 (en) 2013-06-26 2014-12-31 Abengoa Bioenergia Nuevas Tecnologias S.A. Yeasts engineered for the production of valuable chemicals from sugars
US20180030482A1 (en) 2014-04-18 2018-02-01 Moralco B.V. Use of acetaldehyde in the fermentative production of ethanol
DE102014210308A1 (de) 2014-05-30 2015-12-03 Wacker Chemie Ag Hefestamm zur Produktion von Carotinoiden
WO2017050652A1 (en) 2015-09-25 2017-03-30 Dsm Ip Assets B.V. Asparaginase
CN108884480A (zh) 2015-12-30 2018-11-23 帝斯曼知识产权资产管理有限公司 三酰基甘油的部分酶水解
CN109153979A (zh) 2015-12-30 2019-01-04 帝斯曼知识产权资产管理有限公司 三酰基甘油的部分酶水解
CA3025596A1 (en) 2016-06-10 2017-12-14 Dsm Ip Assets B.V. Mutant lipase and use thereof
WO2018115251A1 (en) 2016-12-21 2018-06-28 Vib Vzw Xylose isomerases that confer efficient xylose fermentation capability to yeast
US11739166B2 (en) 2020-07-02 2023-08-29 Davol Inc. Reactive polysaccharide-based hemostatic agent
CA3235083A1 (en) * 2021-10-15 2023-04-20 The Trustees Of Columbia University In The City Of New York Compositions and methods for treating skin conditions

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4857467A (en) * 1986-07-23 1989-08-15 Phillips Petroleum Company Carbon and energy source markers for transformation of strains of the genes Pichia
FR2635115B1 (fr) * 1988-08-05 1992-04-03 Rhone Poulenc Sante Procede de preparation de la serum albumine humaine a partir d'une levure
FR2649991B2 (fr) * 1988-08-05 1994-03-04 Rhone Poulenc Sante Utilisation de derives stables du plasmide pkd1 pour l'expression et la secretion de proteines heterologues dans les levures du genre kluyveromyces

Also Published As

Publication number Publication date
EP0597035B1 (de) 1998-09-23
WO1993003159A1 (fr) 1993-02-18
EP0531181A1 (de) 1993-03-10
ZA925735B (en) 1993-04-28
US5593858A (en) 1997-01-14
CA2113551A1 (fr) 1993-02-18
DE69227116T2 (de) 1999-03-25
NZ243782A (en) 1994-07-26
DK0597035T3 (da) 1999-02-15
ATE171474T1 (de) 1998-10-15
ES2123006T3 (es) 1999-01-01
FR2679920B1 (de) 1994-08-19
FR2679920A1 (fr) 1993-02-05
AU2474892A (en) 1993-03-02
EP0597035A1 (de) 1994-05-18
USRE37767E1 (en) 2002-06-25
JPH06509234A (ja) 1994-10-20

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Legal Events

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8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: AVENTIS PHARMA S.A., ANTONY, FR

8328 Change in the person/name/address of the agent

Representative=s name: PFENNING MEINIG & PARTNER GBR, 80339 MUENCHEN

8328 Change in the person/name/address of the agent

Representative=s name: MAIWALD PATENTANWALTSGESELLSCHAFT MBH, 80335 MUENC

R071 Expiry of right

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