DE69227116D1 - HIGHLY STABLE RECOMBINANT YEARS FOR THE PRODUCTION OF RECOMBINANT PROTEINS - Google Patents

HIGHLY STABLE RECOMBINANT YEARS FOR THE PRODUCTION OF RECOMBINANT PROTEINS

Info

Publication number
DE69227116D1
DE69227116D1 DE69227116T DE69227116T DE69227116D1 DE 69227116 D1 DE69227116 D1 DE 69227116D1 DE 69227116 T DE69227116 T DE 69227116T DE 69227116 T DE69227116 T DE 69227116T DE 69227116 D1 DE69227116 D1 DE 69227116D1
Authority
DE
Germany
Prior art keywords
recombinant
highly stable
years
production
proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69227116T
Other languages
German (de)
Other versions
DE69227116T2 (en
Inventor
Reinhard Fleer
Alain Fournier
Patrice Yeh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Aventis Pharma SA
Original Assignee
Rhone Poulenc Rorer SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rhone Poulenc Rorer SA filed Critical Rhone Poulenc Rorer SA
Publication of DE69227116D1 publication Critical patent/DE69227116D1/en
Application granted granted Critical
Publication of DE69227116T2 publication Critical patent/DE69227116T2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Abstract

The present invention relates to new recombinant yeasts highly stable in a complex medium, characterised in that they are yeasts of the genus Kluyveromyces in which an essential gene is not functional, and which contain a vector carrying a functional copy of the said gene.
DE69227116T 1991-08-02 1992-08-03 HIGHLY STABLE RECOMBINANT YEARS FOR THE PRODUCTION OF RECOMBINANT PROTEINS Expired - Lifetime DE69227116T2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9109854A FR2679920A1 (en) 1991-08-02 1991-08-02 Highly stable recombinant yeasts for the production of recombinant proteins, their preparation and their use
PCT/FR1992/000769 WO1993003159A1 (en) 1991-08-02 1992-08-03 Highly stable recombinant yeasts for the production of recombinant proteins

Publications (2)

Publication Number Publication Date
DE69227116D1 true DE69227116D1 (en) 1998-10-29
DE69227116T2 DE69227116T2 (en) 1999-03-25

Family

ID=9415866

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69227116T Expired - Lifetime DE69227116T2 (en) 1991-08-02 1992-08-03 HIGHLY STABLE RECOMBINANT YEARS FOR THE PRODUCTION OF RECOMBINANT PROTEINS

Country Status (13)

Country Link
US (2) USRE37767E1 (en)
EP (2) EP0597035B1 (en)
JP (1) JPH06509234A (en)
AT (1) ATE171474T1 (en)
AU (1) AU2474892A (en)
CA (1) CA2113551A1 (en)
DE (1) DE69227116T2 (en)
DK (1) DK0597035T3 (en)
ES (1) ES2123006T3 (en)
FR (1) FR2679920A1 (en)
NZ (1) NZ243782A (en)
WO (1) WO1993003159A1 (en)
ZA (1) ZA925735B (en)

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US6265186B1 (en) 1997-04-11 2001-07-24 Dsm N.V. Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces
AU3731400A (en) 1999-03-05 2000-09-21 Trustees Of University Technology Corporation, The Methods and compositions useful in inhibiting apoptosis
US6849605B1 (en) * 1999-03-05 2005-02-01 The Trustees Of University Technology Corporation Inhibitors of serine protease activity, methods and compositions for treatment of viral infections
US6489308B1 (en) 1999-03-05 2002-12-03 Trustees Of University Of Technology Corporation Inhibitors of serine protease activity, methods and compositions for treatment of nitric-oxide-induced clinical conditions
DE10101962B4 (en) * 2001-01-17 2006-07-27 Tad Pharma Gmbh DNA sequence with regulatory regions for the expression of proteins
CN100434440C (en) * 2002-12-02 2008-11-19 阿布格尼克斯公司 Antibodies directed to tumor necrosis factor and uses thereof
US20040220242A1 (en) * 2003-05-02 2004-11-04 Leland Shapiro Inhibitors of serine protease activity, methods and compositions for treatment of nitric oxide induced clinical conditions
DE102008057451A1 (en) * 2008-11-14 2010-05-20 Martin-Luther-Universität Halle-Wittenberg Method for oral vaccination by means of recombinant yeasts
EP2367928B1 (en) 2008-12-24 2018-03-14 DSM IP Assets B.V. Xylose isomerase genes and their use in fermentation of pentose sugars
UA108853C2 (en) 2009-07-10 2015-06-25 Galactose fermentation method
MX2012012171A (en) 2010-04-21 2013-02-27 Dsm Ip Assets Bv Process for the production of cells which are capable of converting arabinose.
WO2011149353A1 (en) 2010-05-27 2011-12-01 C5 Yeast Company B.V. Yeast strains engineered to produce ethanol from acetic acid and glycerol
EP2576804B1 (en) 2010-05-28 2016-12-14 Codexis, Inc. Pentose fermentation by a recombinant microorganism
EA201300454A1 (en) 2010-10-13 2013-09-30 ДСМ АйПи АССЕТС Б.В. YELLOW CELL THROUGH PENTOSE AND GLUCOSE
EP2663645B1 (en) 2010-11-18 2014-12-17 DSM IP Assets B.V. Yeast strains engineered to produce ethanol from glycerol
WO2012125027A1 (en) 2011-03-14 2012-09-20 Dsm Ip Assets B.V. Yeast strains that ferment uronic acids
EP2691517A4 (en) 2011-03-30 2015-04-22 Codexis Inc Pentose fermentation by a recombinant microorganism
CN103502267A (en) 2011-04-22 2014-01-08 帝斯曼知识产权资产管理有限公司 Yeast cell capable of converting sugars including arabinose and xylose
WO2013003219A1 (en) 2011-06-30 2013-01-03 Codexis, Inc. Pentose fermentation by a recombinant microorganism
EP2546336A1 (en) 2011-07-11 2013-01-16 DSM IP Assets B.V. Yeast strains that consume uronic acids and produce fermentation products such as ethanol
EP2554668A1 (en) 2011-08-04 2013-02-06 DSM IP Assets B.V. A pentose sugar fermenting cell
AR087423A1 (en) 2011-08-04 2014-03-19 Dsm Ip Assets Bv CAPABLE CELL TO FERMENT PENTOUS SUGARS
AU2012346662B2 (en) 2011-11-30 2016-07-28 Dsm Ip Assets B.V. Yeast strains engineered to produce ethanol from acetic acid and glycerol
DK2875119T3 (en) 2012-07-19 2018-04-03 Dsm Ip Assets Bv AGSE-DEFICIENT TRIALS
IN2015DN01042A (en) 2012-08-28 2015-06-26 Dsm Ip Assets Bv
IN2015DN01041A (en) 2012-08-28 2015-06-26 Dsm Ip Assets Bv
AR093025A1 (en) 2012-10-16 2015-05-13 Dsm Ip Assets Bv CELLS WITH IMPROVED PENTOSE CONVERSION
US9611515B2 (en) 2012-11-20 2017-04-04 Codexis, Inc. Pentose fermentation by a recombinant microorganism
US9988615B2 (en) 2013-02-04 2018-06-05 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
WO2014202622A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202624A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202620A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202621A1 (en) 2013-06-20 2014-12-24 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
WO2014207113A1 (en) 2013-06-26 2014-12-31 Abengoa Bioenergia Nuevas Tecnologias S.A. Yeasts engineered for the production of valuable chemicals from sugars
US20180030482A1 (en) 2014-04-18 2018-02-01 Moralco B.V. Use of acetaldehyde in the fermentative production of ethanol
DE102014210308A1 (en) 2014-05-30 2015-12-03 Wacker Chemie Ag Yeast strain for the production of carotenoids
WO2017050652A1 (en) 2015-09-25 2017-03-30 Dsm Ip Assets B.V. Asparaginase
CA3009871A1 (en) 2015-12-30 2017-07-06 Dsm Ip Assets B.V. Partial enzymatic hydrolysis of triacylglycerols
US11441099B2 (en) 2015-12-30 2022-09-13 Dsm Ip Assets B.V. Partial enzymatic hydrolysis of triacylglycerols
WO2017211930A1 (en) 2016-06-10 2017-12-14 Dsm Ip Assets B.V. Mutant lipase and use thereof
EP3559210A1 (en) 2016-12-21 2019-10-30 Vib Vzw Xylose isomerases that confer efficient xylose fermentation capability to yeast
US11739166B2 (en) 2020-07-02 2023-08-29 Davol Inc. Reactive polysaccharide-based hemostatic agent
CA3235083A1 (en) * 2021-10-15 2023-04-20 The Trustees Of Columbia University In The City Of New York Compositions and methods for treating skin conditions

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4857467A (en) * 1986-07-23 1989-08-15 Phillips Petroleum Company Carbon and energy source markers for transformation of strains of the genes Pichia
FR2635115B1 (en) * 1988-08-05 1992-04-03 Rhone Poulenc Sante PROCESS FOR THE PREPARATION OF HUMAN ALBUMIN SERUM FROM YEAST
FR2649991B2 (en) * 1988-08-05 1994-03-04 Rhone Poulenc Sante USE OF STABLE DERIVATIVES OF PLASMID PKD1 FOR THE EXPRESSION AND SECRETION OF HETEROLOGOUS PROTEINS IN YEASTS OF THE GENUS KLUYVEROMYCES

Also Published As

Publication number Publication date
ES2123006T3 (en) 1999-01-01
JPH06509234A (en) 1994-10-20
USRE37767E1 (en) 2002-06-25
AU2474892A (en) 1993-03-02
NZ243782A (en) 1994-07-26
ZA925735B (en) 1993-04-28
DK0597035T3 (en) 1999-02-15
EP0597035B1 (en) 1998-09-23
EP0531181A1 (en) 1993-03-10
FR2679920A1 (en) 1993-02-05
WO1993003159A1 (en) 1993-02-18
CA2113551A1 (en) 1993-02-18
FR2679920B1 (en) 1994-08-19
EP0597035A1 (en) 1994-05-18
ATE171474T1 (en) 1998-10-15
DE69227116T2 (en) 1999-03-25
US5593858A (en) 1997-01-14

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: AVENTIS PHARMA S.A., ANTONY, FR

8328 Change in the person/name/address of the agent

Representative=s name: PFENNING MEINIG & PARTNER GBR, 80339 MUENCHEN

8328 Change in the person/name/address of the agent

Representative=s name: MAIWALD PATENTANWALTSGESELLSCHAFT MBH, 80335 MUENC

R071 Expiry of right

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