KR960023057A - 니트릴라제 유전자 발현조절인자 및 그 유전자 - Google Patents
니트릴라제 유전자 발현조절인자 및 그 유전자 Download PDFInfo
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- KR960023057A KR960023057A KR1019950061485A KR19950061485A KR960023057A KR 960023057 A KR960023057 A KR 960023057A KR 1019950061485 A KR1019950061485 A KR 1019950061485A KR 19950061485 A KR19950061485 A KR 19950061485A KR 960023057 A KR960023057 A KR 960023057A
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- South Korea
- Prior art keywords
- dna
- seq
- polypeptide
- promoter
- amino acid
- Prior art date
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- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 8
- 108010033272 Nitrilase Proteins 0.000 title claims 2
- 230000014509 gene expression Effects 0.000 title 1
- 229920001184 polypeptide Polymers 0.000 claims abstract 5
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract 5
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract 5
- 241000316848 Rhodococcus <scale insect> Species 0.000 claims abstract 4
- 125000003275 alpha amino acid group Chemical group 0.000 claims abstract 4
- 244000005700 microbiome Species 0.000 claims abstract 4
- 150000002825 nitriles Chemical class 0.000 claims abstract 4
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims abstract 2
- 108700039691 Genetic Promoter Regions Proteins 0.000 claims abstract 2
- 230000003213 activating effect Effects 0.000 claims abstract 2
- 239000013612 plasmid Substances 0.000 claims description 6
- 230000003362 replicative effect Effects 0.000 claims 2
- 230000004913 activation Effects 0.000 claims 1
- 230000003190 augmentative effect Effects 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 claims 1
- 230000037430 deletion Effects 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
본 발명은 니트릴라제 유전자 촉진제를 활성화하기 위한 SEQ ID NO : 1의 아미노산 배열을 갖는 폴리펩타이드와 SEQ ID NO : 2의 아미노산 배열을 갖는 폴리펩타이드의 2개 성분으로 된 조절인자 및 그 부호화 DNA에 관한 것이다. 니트릴라제는 로도코카스 속의 미생물내에 촉진제 영역을 함유하는 니트릴라제 유전자와 함께 본 조절인자를 위한 유전자 부호화를 도임합으로써 제조할 수가 있다.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 본 발명의 조절인자용 유전자부호화 및 니트릴라제용 유전자부호화의 위치와 방향을 SK92로부터의 DNA단편(fragment)상의 화살표로 나타낸 결손 플라스미드(deletion Plasmid)의 개략도,
제2도는 재조합 플라스미드 PSK108의 제한효소지도.
Claims (6)
- SEQ ID NO : 1의 아미노산 배열을 갖는 폴리펩타이드와 SEQ ID NO : 2의 아미노산 배열을 갖는 폴리펩타이드의 2개 성분으로 되며, 니트릴라제 유전자 촉진제를 활성화하는 조절인인자 및 그 부호화 DNA.
- 제1항에 있어서, 폴리펩타이드에 대한 유전자 부호화는 SEQ ID : 3 및 4의 누클리오티드 배열을 가지는 조절인자 및 DNA.
- 제1항 또는 2항에 있어서, 니트릴라제 유전자촉진제의 활성화는 니트릴의 존재하에서 증대하는 조절인자 및 DNA.
- 제1항, 2항, 또는 3항의 조절인자를 위한 부호화 DNA를 함유한 재조합플라스미드 촉진제영역 및 로도코커스 속에 속하는 미생물의 세포를 복제할 수 있는 DNA영역을 함유한 니트릴라제 유전자.
- 제4항에 있어서, 로도코커스 속에 속하는 미생물의 세포를 복제하는 DNA영역을 플라스미드 PRC001, PRC002, PRC003과 PRC004로부터 선택한 재조합 플라스미드.
- 제4항 또는 제5항의 재조합플라스미드에 의해 전환된 로도코커스 속에 속하는 미생물.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP94-337652 | 1994-12-28 | ||
| JP33765294A JP3154633B2 (ja) | 1994-12-28 | 1994-12-28 | ニトリラーゼ遺伝子発現のための調節因子およびその遺伝子 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR960023057A true KR960023057A (ko) | 1996-07-18 |
| KR100358532B1 KR100358532B1 (ko) | 2003-03-28 |
Family
ID=18310677
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019950061485A KR100358532B1 (ko) | 1994-12-28 | 1995-12-28 | 니트릴라제유전자프로모터를활성화하는조절인자및그것을코딩하는dna |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US5602014A (ko) |
| EP (1) | EP0719862B1 (ko) |
| JP (1) | JP3154633B2 (ko) |
| KR (1) | KR100358532B1 (ko) |
| CN (1) | CN1080306C (ko) |
| DE (1) | DE69522192T2 (ko) |
| TW (1) | TW387895B (ko) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3709422B2 (ja) * | 1995-07-21 | 2005-10-26 | 昌 清水 | ニトリラーゼ遺伝子発現に関わる調節因子およびその遺伝子 |
| EP0874905A1 (en) * | 1995-12-22 | 1998-11-04 | Smithkline Beecham Plc | Novel tcts response regulator of staphylococcus aureus |
| GB9713666D0 (en) * | 1997-06-27 | 1997-09-03 | Univ Cambridge Tech | Biosensor materials and methods |
| JPH1175858A (ja) * | 1997-09-05 | 1999-03-23 | Japan Tobacco Inc | 新規核酸及びそれを用いた病原性キサントモナス・カンペストリスの検出方法 |
| DE19848129A1 (de) | 1998-10-19 | 2000-04-20 | Basf Ag | Verfahren zur Herstellung chiraler Carbonsäuren aus Nitrilen mit Hilfe einer Nitrilase oder Mikroorganismen, die ein Gen für die Nitrilase enthalten |
| US20040002147A1 (en) * | 1999-12-29 | 2004-01-01 | Desantis Grace | Nitrilases |
| EP1280892B1 (en) | 2000-03-31 | 2011-01-12 | E.I. Du Pont De Nemours And Company | ISOLATION AND EXPRESSION OF A GENE FOR A NITRILASE FROM i ACIDOVORAX FACILIS 72W |
| WO2002029034A2 (en) | 2000-09-30 | 2002-04-11 | North Carolina State University | Exbb-exbd-tonb gene cluster of pseudomonas putida dot-t1e conferring tolerance to aromatic compounds and resistance to antibiotics |
| US7985572B2 (en) * | 2003-02-27 | 2011-07-26 | Basf Se | Modified nitrilases and their use in methods for the production of carboxylic acids |
| US7148051B2 (en) * | 2004-08-16 | 2006-12-12 | E. I. Du Pont De Nemours And Company | Production of 3-hydroxycarboxylic acid using nitrilase |
| JP2011217665A (ja) * | 2010-04-08 | 2011-11-04 | Mitsubishi Rayon Co Ltd | ニトリルヒドラターゼ遺伝子を置換した微生物 |
| US8697383B2 (en) | 2010-05-25 | 2014-04-15 | Mitsubishi Rayon Co., Ltd. | Fluorescent substrate for detection of enzymatic activity of nitrile-related enzyme |
| JP6156442B2 (ja) * | 2015-05-28 | 2017-07-05 | 三菱ケミカル株式会社 | ニトリルヒドラターゼ遺伝子を置換した微生物 |
| CN113025601B (zh) * | 2019-12-25 | 2024-06-21 | 上海奥博生物医药股份有限公司 | 腈水解酶启动子优化表达及应用 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4920054A (en) * | 1986-07-25 | 1990-04-24 | Allelix, Inc. | Shuttle vectors from rhodococcus equi |
| AU627648B2 (en) * | 1990-02-28 | 1992-08-27 | Teruhiko Beppu | Dna fragment encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant |
| EP0502476B1 (en) * | 1991-03-04 | 2001-07-18 | Mitsubishi Rayon Co., Ltd. | Hybrid plasmid vectors containing genes encoding nitrile degrading enzymes and methods of producing amides and acids |
-
1994
- 1994-12-28 JP JP33765294A patent/JP3154633B2/ja not_active Expired - Lifetime
-
1995
- 1995-12-22 US US08/577,184 patent/US5602014A/en not_active Expired - Lifetime
- 1995-12-27 DE DE69522192T patent/DE69522192T2/de not_active Expired - Lifetime
- 1995-12-27 EP EP95309454A patent/EP0719862B1/en not_active Expired - Lifetime
- 1995-12-28 KR KR1019950061485A patent/KR100358532B1/ko not_active IP Right Cessation
- 1995-12-28 CN CN95119459A patent/CN1080306C/zh not_active Expired - Lifetime
-
1996
- 1996-01-30 TW TW085101136A patent/TW387895B/zh not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| US5602014A (en) | 1997-02-11 |
| DE69522192D1 (de) | 2001-09-20 |
| DE69522192T2 (de) | 2002-04-18 |
| TW387895B (en) | 2000-04-21 |
| CN1133341A (zh) | 1996-10-16 |
| JP3154633B2 (ja) | 2001-04-09 |
| CN1080306C (zh) | 2002-03-06 |
| EP0719862B1 (en) | 2001-08-16 |
| KR100358532B1 (ko) | 2003-03-28 |
| EP0719862A3 (en) | 1998-02-25 |
| JPH08173169A (ja) | 1996-07-09 |
| EP0719862A2 (en) | 1996-07-03 |
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