DE69224269D1 - Biosynthetisches Verfahren zur Herstellung von Protein - Google Patents

Biosynthetisches Verfahren zur Herstellung von Protein

Info

Publication number
DE69224269D1
DE69224269D1 DE69224269T DE69224269T DE69224269D1 DE 69224269 D1 DE69224269 D1 DE 69224269D1 DE 69224269 T DE69224269 T DE 69224269T DE 69224269 T DE69224269 T DE 69224269T DE 69224269 D1 DE69224269 D1 DE 69224269D1
Authority
DE
Germany
Prior art keywords
protein
epib
host
dna
auxiliary
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69224269T
Other languages
English (en)
Other versions
DE69224269T2 (de
Inventor
Karl-Dieter Dr Entian
Friedrich Dr Goetz
Norbert Dr Schnell
Johannes Dr Augustin
Germar Dr Engelke
Ralf Dr Rosenstein
Cortina Dr Kaletta
Cora Dr Klein
Bernd Dr Wieland
Thomas Dr Kupke
Guenther Dr Jung
Roland Dr Kellner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim Pharma GmbH and Co KG
Original Assignee
Dr Karl Thomae GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dr Karl Thomae GmbH filed Critical Dr Karl Thomae GmbH
Publication of DE69224269D1 publication Critical patent/DE69224269D1/de
Application granted granted Critical
Publication of DE69224269T2 publication Critical patent/DE69224269T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
    • C07K2319/92—Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cephalosporin Compounds (AREA)
  • Epoxy Compounds (AREA)
  • Polymers With Sulfur, Phosphorus Or Metals In The Main Chain (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Compounds Of Unknown Constitution (AREA)
DE69224269T 1991-10-31 1992-10-30 Biosynthetisches Verfahren zur Herstellung von Protein Expired - Lifetime DE69224269T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US78423491A 1991-10-31 1991-10-31

Publications (2)

Publication Number Publication Date
DE69224269D1 true DE69224269D1 (de) 1998-03-05
DE69224269T2 DE69224269T2 (de) 1998-06-04

Family

ID=25131778

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69224269T Expired - Lifetime DE69224269T2 (de) 1991-10-31 1992-10-30 Biosynthetisches Verfahren zur Herstellung von Protein

Country Status (18)

Country Link
EP (1) EP0543195B1 (de)
JP (1) JP4117033B2 (de)
KR (1) KR100242268B1 (de)
AT (1) ATE162852T1 (de)
AU (1) AU661054B2 (de)
CA (1) CA2081704C (de)
CZ (2) CZ359396A3 (de)
DE (1) DE69224269T2 (de)
DK (1) DK0543195T3 (de)
ES (1) ES2112289T3 (de)
FI (1) FI104498B (de)
GR (1) GR3026465T3 (de)
HU (1) HU215548B (de)
IL (1) IL103603A (de)
MX (1) MX9206265A (de)
NO (1) NO307474B1 (de)
NZ (1) NZ244976A (de)
ZA (1) ZA928397B (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5662898A (en) * 1990-08-20 1997-09-02 Ciba-Geigy Corporation Genes for the synthesis of antipathogenic substances
US5639949A (en) * 1990-08-20 1997-06-17 Ciba-Geigy Corporation Genes for the synthesis of antipathogenic substances
US6117670A (en) * 1994-06-08 2000-09-12 Novartis Finance Corporation Pyrrolnitrin biosynthesis genes and uses thereof
US6964760B2 (en) 1997-06-10 2005-11-15 University Of Florida Research Foundation Antimicrobial polypeptide, nucleic acid, and methods of use
US5932469A (en) 1997-06-10 1999-08-03 University Of Florida Antimicrobial polypeptide, nucleic acid, and methods of use
US6475771B1 (en) 1997-06-10 2002-11-05 University Of Florida Research Foundation Antimicrobial polypeptides and methods of use
US6861236B2 (en) 2002-05-24 2005-03-01 Applied Nanosystems B.V. Export and modification of (poly)peptides in the lantibiotic way
DE10261782A1 (de) * 2002-12-19 2004-07-01 Dr. Petry Genmedics Gmbh Neue Gene und Genprodukte
DK1819816T3 (da) 2004-12-07 2009-01-26 Applied Nanosystems Bv Fremgangsmåder til fremstilling og udskillelse af modificerede peptider
CN115261274B (zh) * 2022-08-05 2023-11-21 天康制药股份有限公司 一种表皮葡萄球菌高密度发酵培养基及其发酵方法和应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8811761D0 (en) * 1988-05-18 1988-06-22 Thomae Gmbh Dr K Biosynthetic process for preparation of chemical compounds

Also Published As

Publication number Publication date
EP0543195B1 (de) 1998-01-28
CZ284861B6 (cs) 1999-03-17
NO307474B1 (no) 2000-04-10
EP0543195A2 (de) 1993-05-26
EP0543195A3 (en) 1994-06-08
AU661054B2 (en) 1995-07-13
ATE162852T1 (de) 1998-02-15
NZ244976A (en) 1995-07-26
CZ286727B6 (cs) 2000-06-14
ES2112289T3 (es) 1998-04-01
HU215548B (hu) 1999-01-28
HUT65492A (en) 1994-06-28
GR3026465T3 (en) 1998-06-30
DK0543195T3 (da) 1998-09-23
FI924909A (fi) 1993-05-01
KR930008147A (ko) 1993-05-21
IL103603A0 (en) 1993-03-15
CZ359396A3 (cs) 1999-03-17
NO924192L (no) 1993-05-03
MX9206265A (es) 1994-05-31
CA2081704C (en) 2007-05-01
ZA928397B (en) 1994-05-02
IL103603A (en) 1999-12-31
JP4117033B2 (ja) 2008-07-09
FI104498B (fi) 2000-02-15
CZ326992A3 (en) 1993-09-15
CA2081704A1 (en) 1993-05-01
DE69224269T2 (de) 1998-06-04
FI924909A0 (fi) 1992-10-29
NO924192D0 (no) 1992-10-30
KR100242268B1 (ko) 2000-02-01
AU2745992A (en) 1993-05-27
HU9203434D0 (en) 1993-01-28
JPH05292952A (ja) 1993-11-09

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