ES2112289T3 - Procedimiento de biosintesis para la preparacion de proteinas. - Google Patents

Procedimiento de biosintesis para la preparacion de proteinas.

Info

Publication number
ES2112289T3
ES2112289T3 ES92118598T ES92118598T ES2112289T3 ES 2112289 T3 ES2112289 T3 ES 2112289T3 ES 92118598 T ES92118598 T ES 92118598T ES 92118598 T ES92118598 T ES 92118598T ES 2112289 T3 ES2112289 T3 ES 2112289T3
Authority
ES
Spain
Prior art keywords
protein
epib
host
dna
auxiliary
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92118598T
Other languages
English (en)
Inventor
Karl-Dieter Dr Entian
Friedrich Dr Gotz
Norbert Dr Schnell
Johannes Dr Augustin
Germar Dr Engelke
Ralf Dr Rosenstein
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim Pharma GmbH and Co KG
Original Assignee
Dr Karl Thomae GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dr Karl Thomae GmbH filed Critical Dr Karl Thomae GmbH
Application granted granted Critical
Publication of ES2112289T3 publication Critical patent/ES2112289T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
    • C07K2319/92—Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain

Abstract

SE DESCRIBE UN ANFITRION BACTERIANO QUE SE TRANSFORMA MEDIANTE UN PLASMIDO QUE CODIFICA UN PRECURSOR POLIPEPTIDO EN DONDE EL ANFITRION COMPRENDE UN COMPLEJO MULTIENZIMA CAPAZ DE REACCIONAR CON EL PRECURSOR POLIPEPTIDO EXPRESADO PARA PRODUCIR UN POLIPEPTIDO QUE COMPRENDA AL MENOS UN DESHIDROAMINOACIDO Y/O AL MENOS UN PUENTE DE LANTIONA. TAMBIEN SE PRESENTA UN METODO PARA PRODUCIR UN POLIPEPTIDO QUE COMPRENDA AL MENOS UN DESHIDROAMINOACIDO Y/O AL MENOS UN PUENTE DE LANTIONA, TAL COMO LA GALIDERMINA. ADICIONALMENTE SE DESCRIBE UN PLASMIDO CAPAZ DE TRANSFORMAR UN ANFITRION BACTERIANO. TAMBIEN SE HAN DESCUBIERTO MOLECULAS DE DNA RECOMBINANTE QUE ESPECIFICAN LAS ENZIMAS EPI B, EPI C, EPI D, EPI P Y EPI Q QUE ESTAN INVOLUCRADAS EN LA BIOSINTESIS DE LA EPIDERMINA LANTIBIOTICA.
ES92118598T 1991-10-31 1992-10-30 Procedimiento de biosintesis para la preparacion de proteinas. Expired - Lifetime ES2112289T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US78423491A 1991-10-31 1991-10-31

Publications (1)

Publication Number Publication Date
ES2112289T3 true ES2112289T3 (es) 1998-04-01

Family

ID=25131778

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92118598T Expired - Lifetime ES2112289T3 (es) 1991-10-31 1992-10-30 Procedimiento de biosintesis para la preparacion de proteinas.

Country Status (18)

Country Link
EP (1) EP0543195B1 (es)
JP (1) JP4117033B2 (es)
KR (1) KR100242268B1 (es)
AT (1) ATE162852T1 (es)
AU (1) AU661054B2 (es)
CA (1) CA2081704C (es)
CZ (2) CZ359396A3 (es)
DE (1) DE69224269T2 (es)
DK (1) DK0543195T3 (es)
ES (1) ES2112289T3 (es)
FI (1) FI104498B (es)
GR (1) GR3026465T3 (es)
HU (1) HU215548B (es)
IL (1) IL103603A (es)
MX (1) MX9206265A (es)
NO (1) NO307474B1 (es)
NZ (1) NZ244976A (es)
ZA (1) ZA928397B (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5662898A (en) * 1990-08-20 1997-09-02 Ciba-Geigy Corporation Genes for the synthesis of antipathogenic substances
US5639949A (en) * 1990-08-20 1997-06-17 Ciba-Geigy Corporation Genes for the synthesis of antipathogenic substances
US6117670A (en) * 1994-06-08 2000-09-12 Novartis Finance Corporation Pyrrolnitrin biosynthesis genes and uses thereof
US6964760B2 (en) 1997-06-10 2005-11-15 University Of Florida Research Foundation Antimicrobial polypeptide, nucleic acid, and methods of use
US5932469A (en) 1997-06-10 1999-08-03 University Of Florida Antimicrobial polypeptide, nucleic acid, and methods of use
US6475771B1 (en) 1997-06-10 2002-11-05 University Of Florida Research Foundation Antimicrobial polypeptides and methods of use
US6861236B2 (en) 2002-05-24 2005-03-01 Applied Nanosystems B.V. Export and modification of (poly)peptides in the lantibiotic way
DE10261782A1 (de) * 2002-12-19 2004-07-01 Dr. Petry Genmedics Gmbh Neue Gene und Genprodukte
CA2594151C (en) 2004-12-07 2013-02-19 Applied Nanosystems B.V. Methods for the production and secretion of modified peptides
CN117511763A (zh) * 2022-08-05 2024-02-06 天康制药股份有限公司 一种表皮葡萄球菌高密度发酵培养基及其发酵方法和应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8811761D0 (en) * 1988-05-18 1988-06-22 Thomae Gmbh Dr K Biosynthetic process for preparation of chemical compounds

Also Published As

Publication number Publication date
MX9206265A (es) 1994-05-31
ZA928397B (en) 1994-05-02
CZ284861B6 (cs) 1999-03-17
CA2081704A1 (en) 1993-05-01
JP4117033B2 (ja) 2008-07-09
DE69224269D1 (de) 1998-03-05
KR100242268B1 (ko) 2000-02-01
FI924909A0 (fi) 1992-10-29
IL103603A0 (en) 1993-03-15
HUT65492A (en) 1994-06-28
EP0543195B1 (en) 1998-01-28
HU9203434D0 (en) 1993-01-28
ATE162852T1 (de) 1998-02-15
NO307474B1 (no) 2000-04-10
CZ326992A3 (en) 1993-09-15
AU2745992A (en) 1993-05-27
GR3026465T3 (en) 1998-06-30
CZ359396A3 (cs) 1999-03-17
KR930008147A (ko) 1993-05-21
NZ244976A (en) 1995-07-26
EP0543195A3 (en) 1994-06-08
JPH05292952A (ja) 1993-11-09
NO924192L (no) 1993-05-03
NO924192D0 (no) 1992-10-30
FI104498B (fi) 2000-02-15
CA2081704C (en) 2007-05-01
EP0543195A2 (en) 1993-05-26
CZ286727B6 (cs) 2000-06-14
AU661054B2 (en) 1995-07-13
DE69224269T2 (de) 1998-06-04
IL103603A (en) 1999-12-31
FI924909A (fi) 1993-05-01
DK0543195T3 (da) 1998-09-23
HU215548B (hu) 1999-01-28

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