JPS6474988A - Synthetic gene of cystatin c, corresponding recombinant plasmid and recombinant - Google Patents
Synthetic gene of cystatin c, corresponding recombinant plasmid and recombinantInfo
- Publication number
- JPS6474988A JPS6474988A JP22847787A JP22847787A JPS6474988A JP S6474988 A JPS6474988 A JP S6474988A JP 22847787 A JP22847787 A JP 22847787A JP 22847787 A JP22847787 A JP 22847787A JP S6474988 A JPS6474988 A JP S6474988A
- Authority
- JP
- Japan
- Prior art keywords
- gene
- cystatin
- recombinant
- fragment
- amino acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/8139—Cysteine protease (E.C. 3.4.22) inhibitors, e.g. cystatin
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
PURPOSE:To produce protein of cystatin C by using recombinant DNA technology, by chemically or biochemically synthesizing a gene of cystatin C based on a well-known amino acid sequence and using the gene. CONSTITUTION:Based on a previously well-known amino acid sequence, three points of (i) the most acceptable codon is selected in Escherichia coli useful in manifestation of synthetic gene, (ii) a palindrome on any fragment or a duplex is avoided as much as possible and (iii) a stop codon and an attached end DNA for insertion to a vector are set besides DNA sequence to code a gene of cystatin C are taken into consideration, 13 fragments are decomposed, a gene is designed, each fragment is synthesized and linked to give a complete gene of cystatin C. The gene is integrated into a proper a manifestation vector to give a recombinant plasmid, by which a host is transformed and the transformant is cultivated to produce and to recover protein of cystatin C.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP22847787A JPS6474988A (en) | 1987-09-14 | 1987-09-14 | Synthetic gene of cystatin c, corresponding recombinant plasmid and recombinant |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP22847787A JPS6474988A (en) | 1987-09-14 | 1987-09-14 | Synthetic gene of cystatin c, corresponding recombinant plasmid and recombinant |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS6474988A true JPS6474988A (en) | 1989-03-20 |
Family
ID=16877086
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP22847787A Pending JPS6474988A (en) | 1987-09-14 | 1987-09-14 | Synthetic gene of cystatin c, corresponding recombinant plasmid and recombinant |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6474988A (en) |
-
1987
- 1987-09-14 JP JP22847787A patent/JPS6474988A/en active Pending
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