JPS6474989A - Dna to code human lysozyme - Google Patents

Dna to code human lysozyme

Info

Publication number
JPS6474989A
JPS6474989A JP22975287A JP22975287A JPS6474989A JP S6474989 A JPS6474989 A JP S6474989A JP 22975287 A JP22975287 A JP 22975287A JP 22975287 A JP22975287 A JP 22975287A JP S6474989 A JPS6474989 A JP S6474989A
Authority
JP
Japan
Prior art keywords
dna
plasmid
human lysozyme
phage
code
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP22975287A
Other languages
Japanese (ja)
Inventor
Kazuo Nakahama
Koji Yoshimura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Takeda Pharmaceutical Co Ltd
Original Assignee
Takeda Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takeda Chemical Industries Ltd filed Critical Takeda Chemical Industries Ltd
Priority to JP22975287A priority Critical patent/JPS6474989A/en
Publication of JPS6474989A publication Critical patent/JPS6474989A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE:To efficiently secrete and to manifest maturation protein of human lysozyme and to produce a large amount of the maturation protein, by cultivating a transformant transformed with DNA to code a signal peptide and the maturation protein of human lysozyme. CONSTITUTION:mRNA is separated from a human lysozyme producing cell, single stranded cDNA is synthesized from the mRNA, then double stranded DNA is synthesized, the cDNA is integrated into a phage or plasmid, a host is transformed with the phage or plasmid and the prepared transformant is cultivated and the phage or plasmid containing the aimed DNA is isolated. The aimed cloned DNA is cut out from the phage or plasmid and subcloned to a proper plasmid to give DNA to code an amino acid sequence shown by the formula. DNA to code various kinds of proteins is bonded to the 3' end of signal peptide of human lysozyme and inserted into the downstream of a promoter of a manifestation vector so that protein can be secreted and produced.
JP22975287A 1987-09-16 1987-09-16 Dna to code human lysozyme Pending JPS6474989A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP22975287A JPS6474989A (en) 1987-09-16 1987-09-16 Dna to code human lysozyme

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP22975287A JPS6474989A (en) 1987-09-16 1987-09-16 Dna to code human lysozyme

Publications (1)

Publication Number Publication Date
JPS6474989A true JPS6474989A (en) 1989-03-20

Family

ID=16897128

Family Applications (1)

Application Number Title Priority Date Filing Date
JP22975287A Pending JPS6474989A (en) 1987-09-16 1987-09-16 Dna to code human lysozyme

Country Status (1)

Country Link
JP (1) JPS6474989A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0949692A2 (en) * 1998-03-30 1999-10-13 International Superconductivity Technology Center Oxide superconductor and method of treating the surface thereof
CN104278017A (en) * 2013-07-11 2015-01-14 上海万特医药科技有限公司 Recombinant expression method of human lysozyme

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0949692A2 (en) * 1998-03-30 1999-10-13 International Superconductivity Technology Center Oxide superconductor and method of treating the surface thereof
EP0949692A3 (en) * 1998-03-30 2004-03-31 International Superconductivity Technology Center Oxide superconductor and method of treating the surface thereof
CN104278017A (en) * 2013-07-11 2015-01-14 上海万特医药科技有限公司 Recombinant expression method of human lysozyme

Similar Documents

Publication Publication Date Title
NZ232742A (en) Yeast heterologous protein production method, vectors, dna constructs and proteins so produced and aprotinin (1-58) so produced
CA2195625A1 (en) Ubiquitin-Lytic Peptide Fusion Gene Constructs, Protein Products Deriving Therefrom, and Methods of Making and Using Same
ES8604252A1 (en) Expression vectors for enhanced production of polypeptides, plasmids containing the vectors, hosts containing the plasmids, products manufactured thereby and related methods.
IE883896L (en) Improved expression and secretion of heterologous proteins in yeast employing truncated alpha-factor leader sequences
GR1005153B (en) Fusion proteins their preparation and use
ATE88501T1 (en) RECOMBINANT DNA EXPRESSION VECTORS.
AU585857B2 (en) Recombinant fusion proteins
IE883612L (en) Vectors and compounds for direct expression of activated human protein c
HUT54213A (en) Process for the enzymatic removal of n-terminal sequence of proteins
HUT46061A (en) Dna sequences encoding hirudin-like protein and process for producing such proteins
EP0945506A4 (en) Novel peptide, novel dna, and novel antibody
AU653526B2 (en) Novel signal sequences
MX9701720A (en) Process for manufacture of a modified collagen-induced platelet aggregation inhibitor pallidipin.
JPS6474989A (en) Dna to code human lysozyme
JPS642572A (en) Polypeptide, dna and use thereof
GB2171703B (en) Expression-secretion vector for gene expression and protein secretion, recombinant dna including the vector, and method of producing proteins by use of the re
ES2112289T3 (en) BIOSYNTHESIS PROCEDURE FOR THE PREPARATION OF PROTEINS.
IL82207A0 (en) Production of peptides,recombinant plasmid used therefor and animal cells transformed therewith
HRP940432B1 (en) Dna sequences encoding biosynthetic insulin precursors and process for preparation of insulin
JPS6427473A (en) Human pancreas-secreting trypsin inhibitor and production thereof
JPS6439990A (en) Fused gene by bonding aequorin gene to functional gene
TW480285B (en) Enzyme for converting a precursor of a polypeptide into the active form that induces IFN-γ production, the process therefor, and the uses thereof
KR900013077A (en) Method of manufacturing glucagon
JPS6486876A (en) Variant human lysozyme
DK196086A (en) PROCEDURE FOR MANUFACTURING A PROTEIN