JPS6486876A - Variant human lysozyme - Google Patents

Variant human lysozyme

Info

Publication number
JPS6486876A
JPS6486876A JP24528487A JP24528487A JPS6486876A JP S6486876 A JPS6486876 A JP S6486876A JP 24528487 A JP24528487 A JP 24528487A JP 24528487 A JP24528487 A JP 24528487A JP S6486876 A JPS6486876 A JP S6486876A
Authority
JP
Japan
Prior art keywords
human lysozyme
lysozyme
variant
gene
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP24528487A
Other languages
Japanese (ja)
Other versions
JPH0829092B2 (en
Inventor
Masakazu Kikuchi
Yoshio Yamamoto
Yoshihisa Taniyama
Morio Ikehara
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
TANPAKU KOGAKU KENKYUSHO KK
Original Assignee
TANPAKU KOGAKU KENKYUSHO KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by TANPAKU KOGAKU KENKYUSHO KK filed Critical TANPAKU KOGAKU KENKYUSHO KK
Priority to JP24528487A priority Critical patent/JPH0829092B2/en
Publication of JPS6486876A publication Critical patent/JPS6486876A/en
Publication of JPH0829092B2 publication Critical patent/JPH0829092B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)

Abstract

PURPOSE:To efficiently secrete a protein having sufficient lysozyme activity into a culture medium by recombinant DNA technique, by using a gene coding a variant polypeptide obtained by changing part of amino acid sequence of human lysozyme. CONSTITUTION:A variant human lysozyme gene coding variant polypeptide obtained by replacing cysteine in 77th and 95th site of amino acid sequence of natural human lysozyme with alanine is prepared and the gene is inserted into a proper manifestation vector together with a nucleotide sequence (preferably obtained by giving modification to nucleotide sequence coding signal peptide of egg white lysozyme) coding signal peptide and host is transformed using the resultant manifestation vector (e.g. plasmid pERI 8716) and the resultant transformant is cultivated and a variant human lysozyme secreted into a culture medium is separated and obtained.
JP24528487A 1987-09-29 1987-09-29 Mutant human lysozyme Expired - Lifetime JPH0829092B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP24528487A JPH0829092B2 (en) 1987-09-29 1987-09-29 Mutant human lysozyme

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP24528487A JPH0829092B2 (en) 1987-09-29 1987-09-29 Mutant human lysozyme

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP20427195A Division JP2538541B2 (en) 1995-08-10 1995-08-10 Gene coding for mutant human lysozyme

Publications (2)

Publication Number Publication Date
JPS6486876A true JPS6486876A (en) 1989-03-31
JPH0829092B2 JPH0829092B2 (en) 1996-03-27

Family

ID=17131376

Family Applications (1)

Application Number Title Priority Date Filing Date
JP24528487A Expired - Lifetime JPH0829092B2 (en) 1987-09-29 1987-09-29 Mutant human lysozyme

Country Status (1)

Country Link
JP (1) JPH0829092B2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5382676A (en) * 1991-08-28 1995-01-17 Daicel Chemical Industries, Ltd. Purified 3,4-epoxycyclohexyl methyl(meth)acrylate, a process for the preparation thereof and a 3,4-epoxycyclohexyl methyl(meth)acrylate composition
US5569773A (en) * 1990-02-15 1996-10-29 Daicel Chemical Industries, Ltd. Process for producing a composition comprising epoxy compounds having hydroxyl group
WO2002070715A1 (en) * 2001-03-02 2002-09-12 Long Yu Human g-type lysozyme, the encoding sequence, preparing method and the uses thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5569773A (en) * 1990-02-15 1996-10-29 Daicel Chemical Industries, Ltd. Process for producing a composition comprising epoxy compounds having hydroxyl group
US5382676A (en) * 1991-08-28 1995-01-17 Daicel Chemical Industries, Ltd. Purified 3,4-epoxycyclohexyl methyl(meth)acrylate, a process for the preparation thereof and a 3,4-epoxycyclohexyl methyl(meth)acrylate composition
WO2002070715A1 (en) * 2001-03-02 2002-09-12 Long Yu Human g-type lysozyme, the encoding sequence, preparing method and the uses thereof

Also Published As

Publication number Publication date
JPH0829092B2 (en) 1996-03-27

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