JPS6437299A - Novel physiologically active polypeptide - Google Patents

Novel physiologically active polypeptide

Info

Publication number
JPS6437299A
JPS6437299A JP62192557A JP19255787A JPS6437299A JP S6437299 A JPS6437299 A JP S6437299A JP 62192557 A JP62192557 A JP 62192557A JP 19255787 A JP19255787 A JP 19255787A JP S6437299 A JPS6437299 A JP S6437299A
Authority
JP
Japan
Prior art keywords
gene
polypeptide
plasmid
formula
acid sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP62192557A
Other languages
Japanese (ja)
Other versions
JPH0817714B2 (en
Inventor
Satoshi Nakamura
Tsukio Sakugi
Kazuo Kitai
Yataro Ichikawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Teijin Ltd
Original Assignee
Teijin Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Teijin Ltd filed Critical Teijin Ltd
Priority to JP62192557A priority Critical patent/JPH0817714B2/en
Publication of JPS6437299A publication Critical patent/JPS6437299A/en
Publication of JPH0817714B2 publication Critical patent/JPH0817714B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/525—Tumour necrosis factor [TNF]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Abstract

NEW MATERIAL:The polypeptide having an amino acid sequence of formula. USE:An antitumor agent. PREPARATION:For example, gene of human tumor necrosis factor (TNF) is chemically synthesized by selecting the amino acids constituting human TNF protein from a designated codon. The gene is linked with a proper vector and closed to obtain an expression-type plasmid. The plasmid is cut with a proper restriction enzyme to remove a specific region in the human TNF gene and the gene is repaired by using an oligonucleotide having another base sequence to obtain a recombinant plasmid containing a DNA region coding a polypeptide having methionine group bonded to an amino terminal. The plasmid is inserted into a microorganism and the transformed microorganism is cultured to obtain the polypeptide having the amino acid sequence of formula.
JP62192557A 1987-08-03 1987-08-03 Novel bioactive polypeptide Expired - Lifetime JPH0817714B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP62192557A JPH0817714B2 (en) 1987-08-03 1987-08-03 Novel bioactive polypeptide

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62192557A JPH0817714B2 (en) 1987-08-03 1987-08-03 Novel bioactive polypeptide

Publications (2)

Publication Number Publication Date
JPS6437299A true JPS6437299A (en) 1989-02-07
JPH0817714B2 JPH0817714B2 (en) 1996-02-28

Family

ID=16293257

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62192557A Expired - Lifetime JPH0817714B2 (en) 1987-08-03 1987-08-03 Novel bioactive polypeptide

Country Status (1)

Country Link
JP (1) JPH0817714B2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991017180A1 (en) * 1990-04-27 1991-11-14 Peptide Technology Ltd Peptides derived from human tumour necrosis factor alpha and useful against intracellular malarial parasites

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991017180A1 (en) * 1990-04-27 1991-11-14 Peptide Technology Ltd Peptides derived from human tumour necrosis factor alpha and useful against intracellular malarial parasites

Also Published As

Publication number Publication date
JPH0817714B2 (en) 1996-02-28

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