RU92004585A - PROMOTOR, DNA SEQUENCE, VECTOR, CELLS, DNA EXPRESSION METHOD - Google Patents

PROMOTOR, DNA SEQUENCE, VECTOR, CELLS, DNA EXPRESSION METHOD

Info

Publication number
RU92004585A
RU92004585A RU92004585/13A RU92004585A RU92004585A RU 92004585 A RU92004585 A RU 92004585A RU 92004585/13 A RU92004585/13 A RU 92004585/13A RU 92004585 A RU92004585 A RU 92004585A RU 92004585 A RU92004585 A RU 92004585A
Authority
RU
Russia
Prior art keywords
dna
promotor
vector
cells
dna sequence
Prior art date
Application number
RU92004585/13A
Other languages
Russian (ru)
Other versions
RU2112802C1 (en
Inventor
Даса Ортега Хосе
Антонио Гил Хосе
Вигал Гарсиа Томас
Франсиско Мартин Хуан
Original Assignee
Лабораториос Сероно С.А.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Лабораториос Сероно С.А. filed Critical Лабораториос Сероно С.А.
Publication of RU92004585A publication Critical patent/RU92004585A/en
Application granted granted Critical
Publication of RU2112802C1 publication Critical patent/RU2112802C1/en

Links

Claims (1)

Предлагаются новый, регулируемый кальцием промотор, который можно использовать для увеличения продуцирования внеклеточных ферментов или гетерологических полипептидов, а также рекомбинантный вектор, который включает ДНК-последовательность, оперативно связанную с ДНК, кодирующей фермент или полипептид, и организм-хозяин, трансформированный рекомбинантным вектором, включающим промотор, оперативно связанный с ДНК, кодирующей фермент или полипептид. Кроме того, настоящее изобретение относится к системам экспрессии Streptomyces и способам экспрессии последовательностей чужеродной ДКН в Streptomyces и секретирования в окружающую среду полипептидов или протеинов, кодируемых этими последовательностями чужеродной ДНК.A novel calcium-regulated promoter is proposed that can be used to increase the production of extracellular enzymes or heterologous polypeptides, as well as a recombinant vector that includes a DNA sequence operably linked to DNA encoding an enzyme or polypeptide, and a host organism transformed with a recombinant vector including a promoter operably linked to DNA encoding an enzyme or polypeptide. Furthermore, the present invention relates to Streptomyces expression systems and methods for expressing foreign DTH sequences in Streptomyces and secreting into the environment polypeptides or proteins encoded by these foreign DNA sequences.
RU92004585A 1991-12-12 1992-12-11 Dna fragment encoding promoter that provides calcium-regulated expression of polypeptides, recombinant expression vector pulad 50, recombinant expression vector pulad 51, recombinant strain streptomyces lividans - a producer of aminoglycoside phosphotransferase, recombinant strain streptomyces lividans - a producer of catecholdeoxygenase, method of expression of foreign dna-sequence RU2112802C1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ES9102765 1991-12-12
ESP9102765 1991-12-12

Publications (2)

Publication Number Publication Date
RU92004585A true RU92004585A (en) 1996-09-20
RU2112802C1 RU2112802C1 (en) 1998-06-10

Family

ID=8274462

Family Applications (1)

Application Number Title Priority Date Filing Date
RU92004585A RU2112802C1 (en) 1991-12-12 1992-12-11 Dna fragment encoding promoter that provides calcium-regulated expression of polypeptides, recombinant expression vector pulad 50, recombinant expression vector pulad 51, recombinant strain streptomyces lividans - a producer of aminoglycoside phosphotransferase, recombinant strain streptomyces lividans - a producer of catecholdeoxygenase, method of expression of foreign dna-sequence

Country Status (15)

Country Link
US (2) US5385841A (en)
EP (1) EP0546833B1 (en)
JP (1) JP3340164B2 (en)
AT (1) ATE154392T1 (en)
AU (1) AU661073B2 (en)
CA (1) CA2084351C (en)
DE (1) DE69220336T2 (en)
DK (1) DK0546833T3 (en)
ES (1) ES2103902T3 (en)
GR (1) GR3024625T3 (en)
IL (1) IL103975A (en)
LT (1) LT3133B (en)
LV (1) LV10728B (en)
RU (1) RU2112802C1 (en)
ZA (1) ZA929284B (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA929284B (en) * 1991-12-12 1993-06-02 Serono Lab P0438, an new calcium-regulated promoter.
US6381353B1 (en) 1996-08-30 2002-04-30 Marvin Weiss System for counting colonies of micro-organisms in petri dishes and other culture media

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8315579D0 (en) 1983-06-07 1983-07-13 Biogen Nv Streptomyces expression systems
US4766066A (en) * 1984-09-27 1988-08-23 Eli Lilly And Company Method of using bacteriophage lambda p1 promoter to produce a functional polypeptide in streptomyces
US4745056A (en) * 1984-10-23 1988-05-17 Biotechnica International, Inc. Streptomyces secretion vector
DE3536182A1 (en) * 1985-10-10 1987-04-16 Hoechst Ag PROMOTOR ARRANGEMENT FOR STREPTOMYCETE VECTORS
US5200327A (en) * 1985-11-06 1993-04-06 Cangene Corporation Expression system for the secretion of bioactive human granulocyte macrophage colony stimulating factor (GM-CSF) and other heterologous proteins from streptomyces
AU1299988A (en) 1987-03-09 1988-10-10 Cetus Corporation Expression of heterologous genes in streptomyces species
US5118617A (en) * 1989-05-19 1992-06-02 Applied Research Systems Ars Holding N.V. Saf polypeptide, gene coding therefor, saf promoter and expression methods for expressing foreign dna sequences in streptomyces
ZA929284B (en) * 1991-12-12 1993-06-02 Serono Lab P0438, an new calcium-regulated promoter.

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