ATE478140T1 - Verfahren zur herstellung sezernierter polypeptide - Google Patents

Verfahren zur herstellung sezernierter polypeptide

Info

Publication number
ATE478140T1
ATE478140T1 AT04751203T AT04751203T ATE478140T1 AT E478140 T1 ATE478140 T1 AT E478140T1 AT 04751203 T AT04751203 T AT 04751203T AT 04751203 T AT04751203 T AT 04751203T AT E478140 T1 ATE478140 T1 AT E478140T1
Authority
AT
Austria
Prior art keywords
nucleotide sequence
polypeptide
signal peptide
fungal host
operably linked
Prior art date
Application number
AT04751203T
Other languages
English (en)
Inventor
Suchindra Maiyuran
Ana Fidantsef
Howard Brody
Original Assignee
Novozymes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Inc filed Critical Novozymes Inc
Application granted granted Critical
Publication of ATE478140T1 publication Critical patent/ATE478140T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT04751203T 2003-05-02 2004-04-30 Verfahren zur herstellung sezernierter polypeptide ATE478140T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46776603P 2003-05-02 2003-05-02
PCT/US2004/013711 WO2004111228A1 (en) 2003-05-02 2004-04-30 Methods for producing secreted polypeptides

Publications (1)

Publication Number Publication Date
ATE478140T1 true ATE478140T1 (de) 2010-09-15

Family

ID=33551405

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04751203T ATE478140T1 (de) 2003-05-02 2004-04-30 Verfahren zur herstellung sezernierter polypeptide

Country Status (8)

Country Link
US (4) US7393664B2 (de)
EP (1) EP1625219B1 (de)
CN (1) CN1836038B (de)
AT (1) ATE478140T1 (de)
DE (1) DE602004028724D1 (de)
DK (1) DK1625219T3 (de)
ES (1) ES2350903T3 (de)
WO (1) WO2004111228A1 (de)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1902310B (zh) * 2003-10-28 2011-09-21 诺维信股份有限公司 具有β-葡糖苷酶活性的多肽和编码所述多肽的多核苷酸
ES2810026T3 (es) 2004-03-25 2021-03-08 Novozymes Inc Métodos para la degradación o la conversión de polisacáridos de la pared celular vegetal
MX2007014229A (es) * 2005-05-19 2008-02-07 Novozymes North America Inc Produccion de enzimas.
US8697364B2 (en) * 2006-06-26 2014-04-15 Dsm Ip Assets B.V. High throughput transfection of filamentous fungi
US8546106B2 (en) * 2006-07-21 2013-10-01 Novozymes, Inc. Methods of increasing secretion of polypeptides having biological activity
WO2009042871A1 (en) * 2007-09-28 2009-04-02 Novozymes A/S Polypeptides having cellobiohydrolase ii activity and polynucleotides encoding same
US9012186B2 (en) 2009-04-27 2015-04-21 The Board Of Trustees Of The University Of Illinois Hemicellulose-degrading enzymes
EP2397491A1 (de) 2010-06-21 2011-12-21 Technische Universität Wien LeaA von Trichoderma reesei
JPWO2012060389A1 (ja) * 2010-11-05 2014-05-12 旭硝子株式会社 シゾサッカロミセス属酵母の形質転換体およびその製造方法
DK2748305T3 (en) 2011-08-24 2017-02-06 Novozymes Inc CELLULOLYTIC ENZYME COMPOSITIONS AND APPLICATIONS THEREOF
CN103184159B (zh) * 2011-12-27 2016-09-21 丰益(上海)生物技术研发中心有限公司 紧密枝顶孢霉菌株及其特异性脂肪酶
EP2708553A1 (de) 2012-09-18 2014-03-19 Technische Universität Wien Modifizierte Pilzzelle
WO2014151805A2 (en) 2013-03-15 2014-09-25 Mascoma Corporation Expression of beta-glucosidases for hydrolysis of lignocellulose and associated oligomers
EP3074513A1 (de) 2013-11-26 2016-10-05 Novozymes A/S Enzymzusammensetzungen und verwendungen davon
WO2015187935A1 (en) 2014-06-06 2015-12-10 Novozymes A/S Enzyme compositions and uses thereof
WO2016145084A1 (en) 2015-03-09 2016-09-15 Novozymes A/S Methods of introducing multiple expression constructs into a eukaryotic cell
CN104975039B (zh) * 2015-06-24 2018-05-11 方诩 一种重组质粒及其在降解纤维素原料中的应用
WO2017073716A1 (ja) * 2015-10-30 2017-05-04 サントリーホールディングス株式会社 Ａｏｂｇｌ３ホモログを用いたステビオール配糖体およびステビオールの製造方法
JP6778208B2 (ja) 2015-10-30 2020-10-28 サントリーホールディングス株式会社 Ａｏｂｇｌ１ホモログを用いたステビオール配糖体およびステビオールの製造方法
US20210284991A1 (en) 2016-11-21 2021-09-16 Novozymes A/S Yeast Cell Extract Assisted Construction of DNA Molecules
JP6839424B2 (ja) * 2016-12-27 2021-03-10 国立大学法人 鹿児島大学 真菌におけるタンパク質の選択的分泌技術
WO2020106600A1 (en) * 2018-11-19 2020-05-28 Basf Se Leader sequence for yeast
CN110669123B (zh) * 2019-09-03 2020-10-13 南京市妇幼保健院 一种分泌型内源性多肽pdff-co3及其应用
CN111378674B (zh) * 2020-03-25 2021-09-10 中国科学院天津工业生物技术研究所 异宗毁丝霉糖化酶MhglaA及其编码基因和在葡萄糖生产中的应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4220721A (en) * 1979-04-27 1980-09-02 University Of Arkansas Foundation Method for enzyme reutilization
DK187280A (da) 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
DE69107455T3 (de) 1990-05-09 2004-09-23 Novozymes A/S Eine ein endoglucanase enzym enthaltende zellulasezubereitung.
US5861271A (en) * 1993-12-17 1999-01-19 Fowler; Timothy Cellulase enzymes and systems for their expressions
AU3634797A (en) * 1996-07-24 1998-02-10 Meiji Seika Kaisha Ltd. Enzyme endoglucanase and cellulase preparations containing the same
US6015703A (en) 1998-03-10 2000-01-18 Iogen Corporation Genetic constructs and genetically modified microbes for enhanced production of beta-glucosidase

Also Published As

Publication number Publication date
US20120122191A1 (en) 2012-05-17
ES2350903T3 (es) 2011-01-28
EP1625219B1 (de) 2010-08-18
US20040248258A1 (en) 2004-12-09
EP1625219A4 (de) 2007-09-05
US20140011262A1 (en) 2014-01-09
US7393664B2 (en) 2008-07-01
US20080268526A1 (en) 2008-10-30
US8497115B2 (en) 2013-07-30
CN1836038A (zh) 2006-09-20
WO2004111228A1 (en) 2004-12-23
DK1625219T3 (da) 2010-11-01
CN1836038B (zh) 2011-04-06
EP1625219A1 (de) 2006-02-15
DE602004028724D1 (de) 2010-09-30

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