ATE318317T1 - Verfahren zur erhöhung der expression von einem interessierenden protein durch rekombinante wirtzellen - Google Patents

Verfahren zur erhöhung der expression von einem interessierenden protein durch rekombinante wirtzellen

Info

Publication number
ATE318317T1
ATE318317T1 AT01928859T AT01928859T ATE318317T1 AT E318317 T1 ATE318317 T1 AT E318317T1 AT 01928859 T AT01928859 T AT 01928859T AT 01928859 T AT01928859 T AT 01928859T AT E318317 T1 ATE318317 T1 AT E318317T1
Authority
AT
Austria
Prior art keywords
expression
interest
protein
increase
host cells
Prior art date
Application number
AT01928859T
Other languages
English (en)
Inventor
Si Lok
Original Assignee
Zymogenetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zymogenetics Inc filed Critical Zymogenetics Inc
Application granted granted Critical
Publication of ATE318317T1 publication Critical patent/ATE318317T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
AT01928859T 2000-04-26 2001-04-25 Verfahren zur erhöhung der expression von einem interessierenden protein durch rekombinante wirtzellen ATE318317T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US55883000A 2000-04-26 2000-04-26

Publications (1)

Publication Number Publication Date
ATE318317T1 true ATE318317T1 (de) 2006-03-15

Family

ID=24231180

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01928859T ATE318317T1 (de) 2000-04-26 2001-04-25 Verfahren zur erhöhung der expression von einem interessierenden protein durch rekombinante wirtzellen

Country Status (7)

Country Link
EP (1) EP1276881B1 (de)
JP (1) JP2003530886A (de)
AT (1) ATE318317T1 (de)
AU (1) AU2001255671A1 (de)
CA (1) CA2407710A1 (de)
DE (1) DE60117375T2 (de)
WO (1) WO2001081596A2 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2189529B1 (de) 2007-08-10 2017-01-11 Toto Ltd. Verfahren zur herstellung rekombinanter säugerzellen und verfahren zur herstellung eines zielproteins
JP5800176B2 (ja) * 2011-02-01 2015-10-28 国立大学法人広島大学 哺乳動物細胞内で目的遺伝子の増幅構造を制御し、高発現させる方法
TWI696700B (zh) 2015-01-16 2020-06-21 國立研究開發法人產業技術總合研究所 使用具有匿跡性之ｒｎａ的基因表現系統及含有該ｒｎａ的基因導入、表現載體
JP6544565B2 (ja) * 2015-04-28 2019-07-17 国立大学法人広島大学 哺乳動物細胞内で目的遺伝子の発現を高める方法およびキット、並びに、その利用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4403036A (en) * 1980-12-02 1983-09-06 University Of Iowa Research Foundation Genetic reagents for generating plasmids containing multiple copies of DNA segments
IL80511A0 (en) * 1985-11-05 1987-02-27 Genetics Inst Multiply amplifiable vectors for high level expression of exogenous dna

Also Published As

Publication number Publication date
WO2001081596A3 (en) 2002-04-18
DE60117375T2 (de) 2006-10-19
CA2407710A1 (en) 2001-11-01
DE60117375D1 (de) 2006-04-27
EP1276881A2 (de) 2003-01-22
WO2001081596A2 (en) 2001-11-01
JP2003530886A (ja) 2003-10-21
AU2001255671A1 (en) 2001-11-07
EP1276881B1 (de) 2006-02-22

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