EA201691696A1 - Способы очистки матричной рнк - Google Patents

Способы очистки матричной рнк

Info

Publication number
EA201691696A1
EA201691696A1 EA201691696A EA201691696A EA201691696A1 EA 201691696 A1 EA201691696 A1 EA 201691696A1 EA 201691696 A EA201691696 A EA 201691696A EA 201691696 A EA201691696 A EA 201691696A EA 201691696 A1 EA201691696 A1 EA 201691696A1
Authority
EA
Eurasian Patent Office
Prior art keywords
mrna
methods
membrane
precipitated
captured
Prior art date
Application number
EA201691696A
Other languages
English (en)
Inventor
Франк Дероза
Ануша Диаз
Шриран Карв
Майкл Хартлейн
Original Assignee
Шир Хьюман Дженетик Терапис, Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=54333288&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EA201691696(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Шир Хьюман Дженетик Терапис, Инк. filed Critical Шир Хьюман Дженетик Терапис, Инк.
Publication of EA201691696A1 publication Critical patent/EA201691696A1/ru

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00Processes for the preparation of sugar derivatives
    • C07H1/06Separation; Purification
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

Abstract

Настоящее изобретение относится, помимо всего прочего, к способам очистки матричной РНК (мРНК), включающим следующие стадии: (а) осаждение мРНК из неочищенного препарата; (б) подвергание неочищенного препарата, содержащего осажденную мРНК, процессу очистки с участием мембранной фильтрации таким образом, что осажденная мРНК захватывается мембраной; и (в) элюирование захваченной осажденной мРНК из мембраны путем повторной солюбилизации мРНК, что приводит к получению очищенного раствора мРНК. В некоторых вариантах реализации изобретения процессом очистки с использованием мембранной фильтрации, подходящим для настоящего изобретения, является тангенциальная поточная фильтрация.
EA201691696A 2014-04-25 2015-04-24 Способы очистки матричной рнк EA201691696A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461984503P 2014-04-25 2014-04-25
PCT/US2015/027563 WO2015164773A1 (en) 2014-04-25 2015-04-24 Methods for purification of messenger rna

Publications (1)

Publication Number Publication Date
EA201691696A1 true EA201691696A1 (ru) 2017-03-31

Family

ID=54333288

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201691696A EA201691696A1 (ru) 2014-04-25 2015-04-24 Способы очистки матричной рнк

Country Status (16)

Country Link
US (6) US9850269B2 (ru)
EP (2) EP3134506B1 (ru)
JP (4) JP6571679B2 (ru)
KR (2) KR102470198B1 (ru)
CN (3) CN110511927A (ru)
AU (2) AU2015249312B2 (ru)
BR (1) BR112016024632A2 (ru)
CA (1) CA2944800A1 (ru)
DK (1) DK3134506T3 (ru)
EA (1) EA201691696A1 (ru)
ES (1) ES2750661T3 (ru)
IL (1) IL247973A0 (ru)
MX (2) MX2016013965A (ru)
PT (1) PT3134506T (ru)
SG (1) SG11201608725YA (ru)
WO (1) WO2015164773A1 (ru)

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