MX2021013954A - Métodos de purificación de arn mensajero. - Google Patents

Métodos de purificación de arn mensajero.

Info

Publication number
MX2021013954A
MX2021013954A MX2021013954A MX2021013954A MX2021013954A MX 2021013954 A MX2021013954 A MX 2021013954A MX 2021013954 A MX2021013954 A MX 2021013954A MX 2021013954 A MX2021013954 A MX 2021013954A MX 2021013954 A MX2021013954 A MX 2021013954A
Authority
MX
Mexico
Prior art keywords
mrna
present
methods
messenger rna
caustic
Prior art date
Application number
MX2021013954A
Other languages
English (en)
Inventor
Frank Derosa
Jonathan Abysalh
Jorel Vargas
Cameron M Smith
Original Assignee
Translate Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Translate Bio Inc filed Critical Translate Bio Inc
Publication of MX2021013954A publication Critical patent/MX2021013954A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity

Abstract

La presente invención proporciona, entre otras cosas, métodos para purificar ARN mensajero (ARNm) de alta calidad adecuados para uso clínico, sin usar solventes cáusticos o inflamables; la presente invención se basa, en parte, en el sorprendente descubrimiento de que el ARNm puede purificarse con éxito mediante precipitación selectiva y lavado sin usar etanol mientras se mantiene la integridad y la alta pureza del ARNm; por lo tanto, la presente invención proporciona un método efectivo, fiable y más seguro para purificar el ARN de aplicaciones terapéuticas a partir de procesos de fabricación a gran escala sin usar ningún solvente cáustico o inflamable.
MX2021013954A 2019-05-15 2020-05-15 Métodos de purificación de arn mensajero. MX2021013954A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962848412P 2019-05-15 2019-05-15
US201962891781P 2019-08-26 2019-08-26
PCT/US2020/033185 WO2020232371A1 (en) 2019-05-15 2020-05-15 Methods for purification of messenger rna

Publications (1)

Publication Number Publication Date
MX2021013954A true MX2021013954A (es) 2022-03-11

Family

ID=70978632

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021013954A MX2021013954A (es) 2019-05-15 2020-05-15 Métodos de purificación de arn mensajero.

Country Status (12)

Country Link
US (2) US11453877B2 (es)
EP (1) EP3969584A1 (es)
JP (1) JP2022532214A (es)
KR (1) KR20220029556A (es)
CN (1) CN114127283A (es)
AU (1) AU2020275885A1 (es)
BR (1) BR112021022910A2 (es)
CA (1) CA3139679A1 (es)
IL (1) IL288022A (es)
MX (1) MX2021013954A (es)
SG (1) SG11202112350XA (es)
WO (1) WO2020232371A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3668979A4 (en) 2017-08-18 2021-06-02 Modernatx, Inc. METHOD OF HPLC ANALYSIS
EP3841208A1 (en) * 2018-08-24 2021-06-30 Translate Bio, Inc. Methods for purification of messenger rna
AU2021351541A1 (en) * 2020-10-01 2023-06-08 Translate Bio, Inc. Methods for purification of messenger rna
CN113584014B (zh) * 2021-07-27 2023-01-20 上海兆维科技发展有限公司 一种rna的制备方法、合成蛋白质的方法以及转录反应液
WO2023150256A1 (en) * 2022-02-03 2023-08-10 Modernatx, Inc. Continuous precipitation for mrna purification

Family Cites Families (24)

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US4500707A (en) 1980-02-29 1985-02-19 University Patents, Inc. Nucleosides useful in the preparation of polynucleotides
US5132418A (en) 1980-02-29 1992-07-21 University Patents, Inc. Process for preparing polynucleotides
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4668777A (en) 1981-03-27 1987-05-26 University Patents, Inc. Phosphoramidite nucleoside compounds
US4415732A (en) 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
US4973679A (en) 1981-03-27 1990-11-27 University Patents, Inc. Process for oligonucleo tide synthesis using phosphormidite intermediates
US4401796A (en) 1981-04-30 1983-08-30 City Of Hope Research Institute Solid-phase synthesis of polynucleotides
US4373071A (en) 1981-04-30 1983-02-08 City Of Hope Research Institute Solid-phase synthesis of polynucleotides
US5153319A (en) 1986-03-31 1992-10-06 University Patents, Inc. Process for preparing polynucleotides
US5047524A (en) 1988-12-21 1991-09-10 Applied Biosystems, Inc. Automated system for polynucleotide synthesis and purification
US5262530A (en) 1988-12-21 1993-11-16 Applied Biosystems, Inc. Automated system for polynucleotide synthesis and purification
US5576196A (en) * 1995-01-13 1996-11-19 Vical Incorporated Process for reducing RNA concentration in a mixture of biological material using diatomaceous earth
US5700642A (en) 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers
US5707812A (en) * 1996-08-06 1998-01-13 Vical Incorporated Purification of plasmid DNA during column chromatography
DE10006591B4 (de) * 2000-02-11 2007-03-29 Eppendorf Ag Verfahren zur Aufreinigung von Nukleinsäuren
ME03327B (me) 2009-12-01 2019-10-20 Translate Bio Inc Stereoidni derivati za isporuku irnk u humanim genetskim oboljenjima
US8853377B2 (en) 2010-11-30 2014-10-07 Shire Human Genetic Therapies, Inc. mRNA for use in treatment of human genetic diseases
BR112015022141A2 (pt) 2013-03-14 2017-08-29 Shire Human Genetic Therapies Método de quantificação de eficiência de capeamento de mrna, kit e método de fabricação de mrna
CA2903487A1 (en) 2013-03-14 2014-09-25 Shire Human Genetic Therapies, Inc. Quantitative assessment for cap efficiency of messenger rna
KR20150128687A (ko) 2013-03-14 2015-11-18 샤이어 휴먼 지네틱 테라피즈 인크. 메신저 rna의 정제 방법
CN117402871A (zh) 2014-04-25 2024-01-16 川斯勒佰尔公司 信使rna的纯化方法
BR112016030852A2 (pt) 2014-07-02 2018-01-16 Shire Human Genetic Therapies encapsulação de rna mensageiro
ES2899323T3 (es) * 2017-02-27 2022-03-10 Translate Bio Inc Métodos de purificación de ARN mensajero
WO2019207061A1 (en) 2018-04-25 2019-10-31 Ethris Gmbh Cryoprotective agents for particulate formulations

Also Published As

Publication number Publication date
WO2020232371A1 (en) 2020-11-19
AU2020275885A1 (en) 2021-12-02
US20230062449A1 (en) 2023-03-02
CA3139679A1 (en) 2020-11-19
US11453877B2 (en) 2022-09-27
IL288022A (en) 2022-01-01
JP2022532214A (ja) 2022-07-13
CN114127283A (zh) 2022-03-01
EP3969584A1 (en) 2022-03-23
SG11202112350XA (en) 2021-12-30
KR20220029556A (ko) 2022-03-08
US20210002635A1 (en) 2021-01-07
BR112021022910A2 (pt) 2022-01-25

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