EA201591229A1 - Способы очистки матричной рнк - Google Patents

Способы очистки матричной рнк

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Publication number
EA201591229A1
EA201591229A1 EA201591229A EA201591229A EA201591229A1 EA 201591229 A1 EA201591229 A1 EA 201591229A1 EA 201591229 A EA201591229 A EA 201591229A EA 201591229 A EA201591229 A EA 201591229A EA 201591229 A1 EA201591229 A1 EA 201591229A1
Authority
EA
Eurasian Patent Office
Prior art keywords
mrna
methods
vitro
purifying
crude preparation
Prior art date
Application number
EA201591229A
Other languages
English (en)
Inventor
Майкл Хартлейн
Франк Дероза
Ануша Диаз
Шриран Карв
Original Assignee
Шир Хьюман Дженетик Терапис, Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Шир Хьюман Дженетик Терапис, Инк. filed Critical Шир Хьюман Дженетик Терапис, Инк.
Publication of EA201591229A1 publication Critical patent/EA201591229A1/ru

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

В настоящем изобретении предлагаются, среди прочего, способы очистки матричной РНК (мРНК), включающие этапы воздействия денатурирующего условия на неочищенный препарат, содержащий мРНК, синтезированную in vitro, и очистки мРНК из неочищенного препарата из этапа (а) путем фильтрации тангенциальным потоком, при этом мРНК, очищенная на этапе (b), по существу, не содержит преждевременно прерванных последовательностей РНК и/или ферментных реагентов, используемых в синтезе in vitro.
EA201591229A 2013-03-14 2014-03-14 Способы очистки матричной рнк EA201591229A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361784996P 2013-03-14 2013-03-14
PCT/US2014/028441 WO2014152966A1 (en) 2013-03-14 2014-03-14 Methods for purification of messenger rna

Publications (1)

Publication Number Publication Date
EA201591229A1 true EA201591229A1 (ru) 2016-01-29

Family

ID=50391557

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201591229A EA201591229A1 (ru) 2013-03-14 2014-03-14 Способы очистки матричной рнк

Country Status (16)

Country Link
US (7) US9957499B2 (ru)
EP (2) EP2970955B1 (ru)
JP (4) JP6586075B2 (ru)
KR (1) KR20150128687A (ru)
CN (1) CN105051190A (ru)
AU (1) AU2014236396A1 (ru)
BR (1) BR112015022660A2 (ru)
CA (1) CA2902892A1 (ru)
DK (1) DK2970955T3 (ru)
EA (1) EA201591229A1 (ru)
ES (1) ES2708561T3 (ru)
IL (1) IL240215A0 (ru)
MX (1) MX2015012865A (ru)
SG (1) SG11201507425RA (ru)
TR (1) TR201901310T4 (ru)
WO (1) WO2014152966A1 (ru)

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Publication number Priority date Publication date Assignee Title
RU2810003C2 (ru) * 2019-02-11 2023-12-21 Этрис Гмбх ОЧИСТКА иРНК ФИЛЬТРАЦИЕЙ В ТАНГЕНЦИАЛЬНОМ ПОТОКЕ

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