BR112015022660A2 - métodos para a purificação de rna mensageiro - Google Patents

métodos para a purificação de rna mensageiro

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Publication number
BR112015022660A2
BR112015022660A2 BR112015022660A BR112015022660A BR112015022660A2 BR 112015022660 A2 BR112015022660 A2 BR 112015022660A2 BR 112015022660 A BR112015022660 A BR 112015022660A BR 112015022660 A BR112015022660 A BR 112015022660A BR 112015022660 A2 BR112015022660 A2 BR 112015022660A2
Authority
BR
Brazil
Prior art keywords
methods
mna
purifying
purification
messenger rna
Prior art date
Application number
BR112015022660A
Other languages
English (en)
Inventor
Dias Anusha
Derosa Frank
Heartlein Michael
Karve Shrirang
Original Assignee
Shire Human Genetic Therapies
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shire Human Genetic Therapies filed Critical Shire Human Genetic Therapies
Publication of BR112015022660A2 publication Critical patent/BR112015022660A2/pt

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

resumo métodos para a purificação de rna mensageiro a presente invenção apresenta, entre outras coisas, métodos para purificação de rna mensageiro (mrna) incluindo as etapas de submeter uma preparação impura que compreende mrna sintetizado in vitro à condição desnaturante, e purificar o mrna a partir da preparação impura da etapa (a) por filtração de fluxo tangencial, em que o mrna purificado a partir da etapa (b) é substancialmente isento de sequências de rna prematuramente abortado e/ou reagentes enzimáticos utilizados na síntese in vitro.
BR112015022660A 2013-03-14 2014-03-14 métodos para a purificação de rna mensageiro BR112015022660A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361784996P 2013-03-14 2013-03-14
PCT/US2014/028441 WO2014152966A1 (en) 2013-03-14 2014-03-14 Methods for purification of messenger rna

Publications (1)

Publication Number Publication Date
BR112015022660A2 true BR112015022660A2 (pt) 2017-10-31

Family

ID=50391557

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015022660A BR112015022660A2 (pt) 2013-03-14 2014-03-14 métodos para a purificação de rna mensageiro

Country Status (17)

Country Link
US (7) US9957499B2 (pt)
EP (3) EP4446413A2 (pt)
JP (4) JP6586075B2 (pt)
KR (1) KR20150128687A (pt)
CN (1) CN105051190A (pt)
AU (1) AU2014236396A1 (pt)
BR (1) BR112015022660A2 (pt)
CA (1) CA2902892A1 (pt)
DK (2) DK2970955T3 (pt)
EA (1) EA201591229A1 (pt)
ES (2) ES2708561T3 (pt)
IL (1) IL240215A0 (pt)
MX (1) MX2015012865A (pt)
PL (1) PL3467108T3 (pt)
SG (1) SG11201507425RA (pt)
TR (1) TR201901310T4 (pt)
WO (1) WO2014152966A1 (pt)

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