TR201901310T4 - Mesajcı RNA'nın saflaştırılması yöntemleri. - Google Patents

Mesajcı RNA'nın saflaştırılması yöntemleri. Download PDF

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Publication number
TR201901310T4
TR201901310T4 TR2019/01310T TR201901310T TR201901310T4 TR 201901310 T4 TR201901310 T4 TR 201901310T4 TR 2019/01310 T TR2019/01310 T TR 2019/01310T TR 201901310 T TR201901310 T TR 201901310T TR 201901310 T4 TR201901310 T4 TR 201901310T4
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TR
Turkey
Prior art keywords
methods
mrna
messenger rna
purification
purifying
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TR2019/01310T
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English (en)
Inventor
Heartlein Michael
Derosa Frank
Dias Anusha
Karve Shrirang
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Translate Bio Inc
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Publication of TR201901310T4 publication Critical patent/TR201901310T4/tr

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

Abstract

Mevcut buluş, diğer şeylerin yanı sıra, in vitro sentezlenen mRNA içeren saf olmayan bir preparasyonu deniştirici koşullara maruz bırakma adımlarını içeren mesajcı RNA'yı (mRNA) saflaştırma yöntemleri ve mRNA'yı adım (a)dan saf olmayan preparasyondan teğetsel akış filtrasyonu ile saflaştırma yöntemleri sağlar ve burada adım (b)den saflaştırılmış mRNA, in vitro sentezde kullanılan erken ayrılmış RNA dizileri ve/veya enzim reaktifleri büyük oranda içermez.
TR2019/01310T 2013-03-14 2014-03-14 Mesajcı RNA'nın saflaştırılması yöntemleri. TR201901310T4 (tr)

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US201361784996P 2013-03-14 2013-03-14

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TR201901310T4 true TR201901310T4 (tr) 2019-02-21

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TR2019/01310T TR201901310T4 (tr) 2013-03-14 2014-03-14 Mesajcı RNA'nın saflaştırılması yöntemleri.

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US (7) US9957499B2 (tr)
EP (2) EP3467108A1 (tr)
JP (4) JP6586075B2 (tr)
KR (1) KR20150128687A (tr)
CN (1) CN105051190A (tr)
AU (1) AU2014236396A1 (tr)
BR (1) BR112015022660A2 (tr)
CA (1) CA2902892A1 (tr)
DK (1) DK2970955T3 (tr)
EA (1) EA201591229A1 (tr)
ES (1) ES2708561T3 (tr)
IL (1) IL240215A0 (tr)
MX (1) MX2015012865A (tr)
SG (1) SG11201507425RA (tr)
TR (1) TR201901310T4 (tr)
WO (1) WO2014152966A1 (tr)

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