ATE526017T1 - Abscheidung von polyelektrolyten und reinigung von antikörpern - Google Patents

Abscheidung von polyelektrolyten und reinigung von antikörpern

Info

Publication number
ATE526017T1
ATE526017T1 AT08705841T AT08705841T ATE526017T1 AT E526017 T1 ATE526017 T1 AT E526017T1 AT 08705841 T AT08705841 T AT 08705841T AT 08705841 T AT08705841 T AT 08705841T AT E526017 T1 ATE526017 T1 AT E526017T1
Authority
AT
Austria
Prior art keywords
polyelectrolytes
antibodies
purification
deposition
polyelectrolyte
Prior art date
Application number
AT08705841T
Other languages
English (en)
Inventor
Robert Fahrner
Jayme Franklin
Paul Mcdonald
Thanmaya Peram
Vikram Sisodiya
Corazon Victa
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of ATE526017T1 publication Critical patent/ATE526017T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
    • A61K31/197—Carboxylic acids, e.g. valproic acid having an amino group the amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine, epsilon-aminocaproic acid or pantothenic acid
    • A61K31/198—Alpha-amino acids, e.g. alanine or edetic acid [EDTA]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/32—Extraction; Separation; Purification by precipitation as complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2878—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2887—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
    • C07K2317/734—Complement-dependent cytotoxicity [CDC]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT08705841T 2007-01-22 2008-01-10 Abscheidung von polyelektrolyten und reinigung von antikörpern ATE526017T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US88606807P 2007-01-22 2007-01-22
US1344607P 2007-12-13 2007-12-13
PCT/US2008/050747 WO2008091740A2 (en) 2007-01-22 2008-01-10 Polyelectrolyte precipitation and purification of antibodies

Publications (1)

Publication Number Publication Date
ATE526017T1 true ATE526017T1 (de) 2011-10-15

Family

ID=39247681

Family Applications (1)

Application Number Title Priority Date Filing Date
AT08705841T ATE526017T1 (de) 2007-01-22 2008-01-10 Abscheidung von polyelektrolyten und reinigung von antikörpern

Country Status (16)

Country Link
US (1) US7947813B2 (de)
EP (2) EP2377527B1 (de)
JP (1) JP5361738B2 (de)
KR (1) KR101431856B1 (de)
CN (1) CN101646431B (de)
AT (1) ATE526017T1 (de)
AU (1) AU2008209404B2 (de)
BR (1) BRPI0806367A2 (de)
CA (1) CA2673851C (de)
ES (2) ES2426158T3 (de)
IL (1) IL199550A (de)
MX (1) MX2009007632A (de)
NZ (1) NZ577933A (de)
RU (1) RU2474585C2 (de)
WO (1) WO2008091740A2 (de)
ZA (1) ZA200904482B (de)

Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7132100B2 (en) 2002-06-14 2006-11-07 Medimmune, Inc. Stabilized liquid anti-RSV antibody formulations
FR2902799B1 (fr) 2006-06-27 2012-10-26 Millipore Corp Procede et unite de preparation d'un echantillon pour l'analyse microbiologique d'un liquide
US9375499B2 (en) 2006-07-14 2016-06-28 Wisconsin Alumni Research Foundation Adsorptive membranes for trapping viruses
US8362217B2 (en) 2006-12-21 2013-01-29 Emd Millipore Corporation Purification of proteins
US8569464B2 (en) 2006-12-21 2013-10-29 Emd Millipore Corporation Purification of proteins
WO2008079302A2 (en) 2006-12-21 2008-07-03 Millipore Corporation Purification of proteins
US8999702B2 (en) 2008-06-11 2015-04-07 Emd Millipore Corporation Stirred tank bioreactor
US8592555B2 (en) 2008-08-11 2013-11-26 Emd Millipore Corporation Immunoglobulin-binding proteins with improved specificity
US20110144311A1 (en) * 2008-08-14 2011-06-16 Rebecca Chmielowski Methods for purifying antibodies using protein a affinity chromatography
CN102165312B (zh) * 2008-09-25 2015-04-22 Jsr株式会社 亲合层析用填充剂
GB0818228D0 (en) * 2008-10-06 2008-11-12 Avecia Biolog Ltd Purification process
SG171446A1 (en) 2008-12-16 2011-07-28 Millipore Corp Stirred tank reactor and method
AU2009331897A1 (en) 2008-12-22 2010-07-01 F. Hoffmann-La Roche Ag Immunoglobulin purification
SG195555A1 (en) 2008-12-24 2013-12-30 Emd Millipore Corp Caustic stable chromatography ligands
US20120022239A1 (en) * 2009-01-13 2012-01-26 Ge Healthcare Bio-Sciences Ab Precipitation of biomolecules with negatively charged polymers
CN102686738A (zh) 2009-09-01 2012-09-19 弗·哈夫曼-拉罗切有限公司 通过改进的a蛋白洗脱增强的蛋白质纯化
SG181496A1 (en) 2009-12-18 2012-07-30 Csl Ltd Method of purifying polypeptides
US8377672B2 (en) 2010-02-18 2013-02-19 3M Innovative Properties Company Ligand functionalized polymers
EP2542889B1 (de) 2010-03-03 2015-03-25 3M Innovative Properties Company Ligandenfunktionalisierte guanidinyl-polymere
CN107312062B (zh) * 2010-05-17 2021-03-16 Emd密理博公司 用于纯化生物分子的刺激响应性聚合物
EP4492053A3 (de) 2010-05-25 2025-03-19 F. Hoffmann-La Roche AG Verfahren zur reinigung von polypeptiden
EP2635599A1 (de) * 2010-11-01 2013-09-11 DSM IP Assets B.V. Antikörperreinigung für einzeleinheit-ionenaustauschchromatograpfie
KR102058254B1 (ko) * 2011-03-29 2019-12-20 글락소스미스클라인 엘엘씨 단백질 정제용 완충제 시스템
IL212911A0 (en) 2011-05-16 2011-07-31 Omrix Biopharmaceuticals Ltd Immunoglobulin reduced in thrombogenic contaminants and preparation thereof
SG186552A1 (en) 2011-06-08 2013-01-30 Emd Millipore Corp Chromatography matrices including novel staphylococcus aureus protein a based ligands
HRP20171272T4 (hr) * 2011-08-02 2020-08-07 Baxalta GmbH Sustavi i metode za povećanje prinosa proteina iz postupka rekombinatne proizvodnje
JP2014527528A (ja) * 2011-08-19 2014-10-16 イー・エム・デイー・ミリポア・コーポレイシヨン 生体分子の精製方法のための小分子の使用
EP2578286A1 (de) 2011-10-04 2013-04-10 Merck Patent GmbH Verfahren und Vorrichtung zur chromatographischen Aufreinigung
EP2656892A1 (de) 2012-04-23 2013-10-30 Merck Patent GmbH Chromatographieverfahren
JP2015519361A (ja) 2012-05-31 2015-07-09 エイジェンシー・フォー・サイエンス，テクノロジー・アンド・リサーチ 正電荷有機添加剤での処理によるタンパク質製剤におけるタンパク質−不純物複合体及び凝集体のレベルを低下させるための方法
CN104507955A (zh) * 2012-05-31 2015-04-08 新加坡科技研究局 采用具有多形式官能性的颗粒对免疫球蛋白g制备物的色谱纯化
SG11201407801VA (en) 2012-05-31 2014-12-30 Agency Science Tech & Res Methods for use of mixed multifunctional surfaces for reducing aggregate content in protein preparations
US20150239956A1 (en) * 2012-06-27 2015-08-27 Asahi Kasei Medical Co., Ltd. High-affinity antibody and method for manufacturing the same
EP2682168A1 (de) 2012-07-02 2014-01-08 Millipore Corporation Reinigung biologischer Moleküle
LT2890710T (lt) 2012-08-28 2017-10-25 Novartis Ag Antikūnų gryninimo būdai, panaudojant alifatinius alkoholius
US20150284447A1 (en) * 2012-11-05 2015-10-08 Medimmune, Llc Method of isolating synagis(r) in the absence of benzonase
SG11201504649RA (en) 2012-12-20 2015-07-30 Merck Patent Gmbh Copolymers for protein precipitation
CN105051055A (zh) 2013-02-06 2015-11-11 新加坡科技研究局 通过使用硫代杂环阳离子处理来降低蛋白质制剂中的聚集物水平的方法
SG10201610955SA (en) * 2013-02-26 2017-02-27 Agency Science Tech & Res Protein purification in the presence of nonionic organic polymers and electropositive surfaces
US9850512B2 (en) 2013-03-15 2017-12-26 The Research Foundation For The State University Of New York Hydrolysis of cellulosic fines in primary clarified sludge of paper mills and the addition of a surfactant to increase the yield
US10779552B2 (en) 2013-07-18 2020-09-22 Chr. Hansen A/S Stabilization of chymosin by polymers
KR20160065092A (ko) * 2013-08-23 2016-06-08 베링거 인겔하임 에르체파우 게엠베하 운트 코 카게 신규 흡착 조성물 및 이의 용도
RU2016112549A (ru) * 2013-09-05 2017-10-10 Дженентек, Инк. Способ для повторного использования хроматографии
RU2020130575A (ru) * 2013-09-24 2020-11-05 Аллерган Фармасьютикалз Интернэшнл Лимитед Способ экстракции белка
GB201318840D0 (en) * 2013-10-24 2013-12-11 Univ Leeds Method and device for protein preparation
EP3071682A1 (de) * 2013-11-20 2016-09-28 CMC Biologics A/S Bioreaktorsystem und verfahren zur herstellung eines biopolymers
EP3102935B1 (de) 2014-02-07 2021-01-27 CMC Biologics A/S Chromatographiesystem und verfahren zur erfassung eines biopolymers
US9951363B2 (en) 2014-03-14 2018-04-24 The Research Foundation for the State University of New York College of Environmental Science and Forestry Enzymatic hydrolysis of old corrugated cardboard (OCC) fines from recycled linerboard mill waste rejects
US10844345B2 (en) 2014-04-16 2020-11-24 Cmc Biologics A/S High density fill and draw fermentation process
US10308679B2 (en) 2014-11-03 2019-06-04 Merck Patent Gmbh Soluble intein fusion proteins and methods for purifying biomolecules
CN107531749A (zh) * 2015-03-06 2018-01-02 豪夫迈·罗氏有限公司 超纯化DsbA和DsbC及其制备和使用方法
JP6823596B2 (ja) 2015-07-31 2021-02-03 中外製薬株式会社 アニオン性ポリマーによる抗体を含有する組成物の精製方法
JP7084301B2 (ja) * 2015-08-21 2022-06-14 エフ．ホフマン－ラ ロシュ アーゲー 低伝導率洗浄緩衝液を用いたアフィニティークロマトグラフィー精製方法
CN111655722A (zh) * 2018-01-31 2020-09-11 瑞泽恩制药公司 用于表征药物产品杂质的系统和方法
WO2020004583A1 (ja) * 2018-06-29 2020-01-02 三菱ケミカル株式会社 ポリペプチドの分離方法、ポリペプチドの製造方法及びポリペプチドの精製装置
JP6564539B1 (ja) * 2018-09-14 2019-08-21 長瀬産業株式会社 スルホン酸化合物によるペプチド精製方法
HU231498B1 (hu) 2019-04-04 2024-05-28 Richter Gedeon Nyrt Immunglobulinok affinitás kromatográfiájának fejlesztése kötést megelőző flokkulálás alkalmazásával
AR119264A1 (es) * 2019-06-05 2021-12-09 Genentech Inc Método para reutilización de cromatografía
WO2021178269A1 (en) 2020-03-02 2021-09-10 Northwestern University Assembly of random copolymer polyelectrolyte complexes for removal of contaminants from water
KR102612555B1 (ko) * 2020-12-18 2023-12-11 한국세라믹기술원 항체결합단백질 및 칼시퀘스트린 융합단백질을 이용한 면역침강 방법
JP2024540909A (ja) * 2021-10-21 2024-11-06 ニクティア テクノロジーズ アーベー 電解質溶液中においてアナライトを他の成分から分離するためのデバイス
CN114574457B (zh) * 2022-01-25 2024-07-23 华东理工大学 一种快速分离纯化蛋白质的方法

Family Cites Families (92)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3252961A (en) * 1961-04-03 1966-05-24 Foremost Dairies Inc Whey process and product
US3737377A (en) * 1971-01-27 1973-06-05 Miles Lab Purification of lactase
US4055469A (en) * 1976-12-10 1977-10-25 Eastman Kodak Company Purification of microbial enzyme extracts using synthetic polyelectrolytes
USRE30985E (en) 1978-01-01 1982-06-29 Serum-free cell culture media
GB2036891B (en) 1978-12-05 1983-05-05 Windsor Smith C Change speed gear
US4515893A (en) 1979-04-26 1985-05-07 Ortho Pharmaceutical Corporation Hybrid cell line for producing complement-fixing monoclonal antibody to human T cells
ES8504461A1 (es) 1982-04-12 1985-04-16 Hybritech Inc Un procedimiento para obtener un polidoma.
US4560655A (en) 1982-12-16 1985-12-24 Immunex Corporation Serum-free cell culture medium and process for making same
US4657866A (en) 1982-12-21 1987-04-14 Sudhir Kumar Serum-free, synthetic, completely chemically defined tissue culture media
GB8308235D0 (en) 1983-03-25 1983-05-05 Celltech Ltd Polypeptides
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
DD266710A3 (de) 1983-06-06 1989-04-12 Ve Forschungszentrum Biotechnologie Verfahren zur biotechnischen Herstellung van alkalischer Phosphatase
US4767704A (en) 1983-10-07 1988-08-30 Columbia University In The City Of New York Protein-free culture medium
US4533496A (en) * 1984-05-08 1985-08-06 Monsanto Company Method of isolating monoclonal antibodies from hybridoma cultures
US5672347A (en) 1984-07-05 1997-09-30 Genentech, Inc. Tumor necrosis factor antagonists and their use
JPS6147500A (ja) 1984-08-15 1986-03-07 Res Dev Corp Of Japan キメラモノクロ−ナル抗体及びその製造法
EP0173494A3 (de) 1984-08-27 1987-11-25 The Board Of Trustees Of The Leland Stanford Junior University Chimäre Rezeptoren durch Verbindung und Expression von DNS
GB8422238D0 (en) 1984-09-03 1984-10-10 Neuberger M S Chimeric proteins
US4879231A (en) 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
JPS61134325A (ja) 1984-12-04 1986-06-21 Teijin Ltd ハイブリツド抗体遺伝子の発現方法
GB8516415D0 (en) 1985-06-28 1985-07-31 Celltech Ltd Culture of animal cells
CA1282069C (en) 1985-09-12 1991-03-26 Damon L. Meyer Antibody complexes of hapten-modified diagnostic or therapeutic agents
EP0247091B1 (de) 1985-11-01 1993-09-29 Xoma Corporation Modulare einheit von antikörpergenen, daraus hergestellte antikörper und verwendung
US5225539A (en) 1986-03-27 1993-07-06 Medical Research Council Recombinant altered antibodies and methods of making altered antibodies
US4927762A (en) 1986-04-01 1990-05-22 Cell Enterprises, Inc. Cell culture medium with antioxidant
GB8610600D0 (en) 1986-04-30 1986-06-04 Novo Industri As Transformation of trichoderma
US4946778A (en) 1987-09-21 1990-08-07 Genex Corporation Single polypeptide chain binding molecules
IL85035A0 (en) 1987-01-08 1988-06-30 Int Genetic Eng Polynucleotide molecule,a chimeric antibody with specificity for human b cell surface antigen,a process for the preparation and methods utilizing the same
US5091313A (en) 1988-08-05 1992-02-25 Tanox Biosystems, Inc. Antigenic epitopes of IgE present on B cell but not basophil surface
US5720937A (en) 1988-01-12 1998-02-24 Genentech, Inc. In vivo tumor detection assay
EP0420937B1 (de) 1988-06-21 1994-11-09 Genentech, Inc. Therapeutische zusammensetzungen für die behandlung von myocard-infarkten
EP0435911B1 (de) 1988-09-23 1996-03-13 Cetus Oncology Corporation Zellenzuchtmedium für erhöhtes zellenwachstum, zur erhöhung der langlebigkeit und expression der produkte
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
EP0402226A1 (de) 1989-06-06 1990-12-12 Institut National De La Recherche Agronomique Transformationsvektoren für Hefe Yarrowia
US5661016A (en) 1990-08-29 1997-08-26 Genpharm International Inc. Transgenic non-human animals capable of producing heterologous antibodies of various isotypes
US5633425A (en) 1990-08-29 1997-05-27 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5545806A (en) 1990-08-29 1996-08-13 Genpharm International, Inc. Ransgenic non-human animals for producing heterologous antibodies
US5625126A (en) 1990-08-29 1997-04-29 Genpharm International, Inc. Transgenic non-human animals for producing heterologous antibodies
JP2938569B2 (ja) 1990-08-29 1999-08-23 ジェンファーム インターナショナル，インコーポレイティド 異種免疫グロブリンを作る方法及びトランスジェニックマウス
US5122469A (en) 1990-10-03 1992-06-16 Genentech, Inc. Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins
EP1260521B1 (de) 1991-08-14 2007-11-21 Genentech Inc. Immunglobulinvarianten für spezifische Fc-epsilon Rezeptoren
WO1993008829A1 (en) 1991-11-04 1993-05-13 The Regents Of The University Of California Compositions that mediate killing of hiv-infected cells
US5525519A (en) * 1992-01-07 1996-06-11 Middlesex Sciences, Inc. Method for isolating biomolecules from a biological sample with linear polymers
CA2140280A1 (en) 1992-08-17 1994-03-03 Avi J. Ashkenazi Bispecific immunoadhesins
ATE161192T1 (de) 1992-08-21 1998-01-15 Genentech Inc Verfahren zur behandlung einer durch lfa-1 vermittelten störung
US5736137A (en) 1992-11-13 1998-04-07 Idec Pharmaceuticals Corporation Therapeutic application of chimeric and radiolabeled antibodies to human B lymphocyte restricted differentiation antigen for treatment of B cell lymphoma
US5595721A (en) 1993-09-16 1997-01-21 Coulter Pharmaceutical, Inc. Radioimmunotherapy of lymphoma using anti-CD20
ES2108566T3 (es) 1993-12-10 1997-12-16 Genentech Inc Procedimientos para diagnosticar alergias y para seleccionar agentes terapeuticos antialergicos.
ATE164079T1 (de) 1994-01-18 1998-04-15 Genentech Inc Verfahren zur behandlung von parasitären infektionen unter verwendung von ige-antagonisten
US5707622A (en) 1994-03-03 1998-01-13 Genentech, Inc. Methods for treating ulcerative colitis
US5922531A (en) * 1994-06-21 1999-07-13 Advanced Research And Technology Polyelectrolyte treated glass for enzyme immobilization and protein purification
DE69534530T2 (de) 1994-08-12 2006-07-06 Immunomedics, Inc. Für b-zell-lymphom und leukämiezellen spezifische immunkonjugate und humane antikörper
JP3901214B2 (ja) * 1994-09-21 2007-04-04 株式会社カネカ インターロイキン類の吸着剤，吸着除去方法および吸着器
IL117645A (en) 1995-03-30 2005-08-31 Genentech Inc Vascular endothelial cell growth factor antagonists for use as medicaments in the treatment of age-related macular degeneration
US5641870A (en) 1995-04-20 1997-06-24 Genentech, Inc. Low pH hydrophobic interaction chromatography for antibody purification
WO1996040210A1 (en) 1995-06-07 1996-12-19 Imclone Systems Incorporated Antibody and antibody fragments for inhibiting the growth of tumors
EP1297847A3 (de) 1996-01-23 2003-05-07 Genentech, Inc. Antikörper gegen CD18 bei Gehirnschlag
US7147851B1 (en) 1996-08-15 2006-12-12 Millennium Pharmaceuticals, Inc. Humanized immunoglobulin reactive with α4β7 integrin
ES2335365T3 (es) 1996-11-27 2010-03-25 Genentech, Inc. Purificacion por afinidad de polipeptido en una matriz de proteina a.
ATE267257T1 (de) 1996-11-27 2004-06-15 Genentech Inc Humanisierte anti-koerper gegen cd11a
US6872568B1 (en) 1997-03-17 2005-03-29 Human Genome Sciences, Inc. Death domain containing receptor 5 antibodies
ES2273415T3 (es) 1997-04-07 2007-05-01 Genentech, Inc. Anticuerpos anti-vegf.
ATE516354T1 (de) 1997-05-15 2011-07-15 Genentech Inc Apo-2-rezeptor
US6265542B1 (en) * 1997-10-24 2001-07-24 Genentech, Inc. Purification of molecules
US6087448A (en) 1997-11-19 2000-07-11 Amcol International Corporation Solid superabsorbent material containing a poly(vinylguanidine) and an acidic water-absorbing resin
KR100960211B1 (ko) * 1998-05-06 2010-05-27 제넨테크, 인크. 이온 교환 크로마토그래피에 의한 단백질 정제 방법
US6217926B1 (en) * 1998-06-30 2001-04-17 Michael Foods, Inc. Aqueous extraction process to selectively remove phospholipid from egg yolks
KR100501817B1 (ko) * 1998-07-21 2005-07-20 몬산토 테크놀로지 엘엘씨 음이온 폴리머성 응집제들을 사용한 단백질 침전물 현탁액의 청징화 방법
US6946129B1 (en) 1999-06-08 2005-09-20 Seattle Genetics, Inc. Recombinant anti-CD40 antibody and uses thereof
CA2387108A1 (en) 1999-10-29 2001-06-07 Genentech, Inc. Anti-prostate stem cell antigen (psca) antibody compositions and methods of use
US20030133939A1 (en) 2001-01-17 2003-07-17 Genecraft, Inc. Binding domain-immunoglobulin fusion proteins
US7321026B2 (en) 2001-06-27 2008-01-22 Skytech Technology Limited Framework-patched immunoglobulins
EP1444249B1 (de) * 2001-11-16 2008-12-10 Norgen Biotek Corporation Verfahren zur proteinreinigung
US20040093621A1 (en) 2001-12-25 2004-05-13 Kyowa Hakko Kogyo Co., Ltd Antibody composition which specifically binds to CD20
AU2003210802B2 (en) 2002-02-05 2009-09-10 Genentech Inc. Protein purification
KR101033196B1 (ko) 2002-02-14 2011-05-09 이뮤노메딕스, 인코오포레이티드 항-ｃｄ20 항체 및 그 융합 단백질 및 이들의 이용방법
WO2004014942A1 (en) 2002-08-09 2004-02-19 Selborne Biological Services (Australia) Pty Limited A method for preparing lipoprotein from a blood source
PT1558648E (pt) 2002-10-17 2012-04-23 Genmab As Anticorpos monoclonais humanos contra cd20
AR042485A1 (es) 2002-12-16 2005-06-22 Genentech Inc Anticuerpo humanizado que se une al cd20 humano
US20040121339A1 (en) * 2002-12-19 2004-06-24 Jizhong Zhou Special film-coated substrate for bio-microarray fabrication and use thereof
DK1581644T3 (da) * 2003-01-09 2007-10-08 Genentech Inc Oprensning af polypeptider
KR101412271B1 (ko) 2003-05-09 2014-06-25 듀크 유니버시티 Ｃｄ２０-특이적 항체 및 이를 이용한 방법
AR044388A1 (es) 2003-05-20 2005-09-07 Applied Molecular Evolution Moleculas de union a cd20
EP3095793B1 (de) * 2003-07-28 2020-03-25 Genentech, Inc. Verringerung des protein-a-auslaugens während einer affinitätschromatografie
WO2005014618A2 (en) 2003-08-08 2005-02-17 Immunomedics, Inc. Bispecific antibodies for inducing apoptosis of tumor and diseased cells
US8147832B2 (en) 2003-08-14 2012-04-03 Merck Patent Gmbh CD20-binding polypeptide compositions and methods
TR201809892T4 (tr) 2003-11-05 2018-07-23 Roche Glycart Ag Fc reseptörüne bağlanma afinitesi ve artırılmış efektör fonksiyonu bulunan antijen bağlayan moleküller.
US20050147581A1 (en) * 2003-11-19 2005-07-07 The Board Of Trustees Of The University Of Illinois Macromolecular drug complexes having improved stability and therapeutic use of the same
ATE437184T1 (de) 2004-01-12 2009-08-15 Applied Molecular Evolution Varianten der fc-region
US7850962B2 (en) 2004-04-20 2010-12-14 Genmab A/S Human monoclonal antibodies against CD20
US7673757B2 (en) * 2006-02-17 2010-03-09 Millipore Corporation Adsorbent filter media for removal of biological contaminants in process liquids
WO2008079302A2 (en) * 2006-12-21 2008-07-03 Millipore Corporation Purification of proteins

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IL199550A (en) 2015-04-30
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RU2009131765A (ru) 2011-02-27
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ES2374330T3 (es) 2012-02-15
ZA200904482B (en) 2010-09-29
WO2008091740A2 (en) 2008-07-31
BRPI0806367A2 (pt) 2011-09-06
MX2009007632A (es) 2009-07-24
AU2008209404B2 (en) 2012-08-16
AU2008209404A1 (en) 2008-07-31
JP2010516773A (ja) 2010-05-20
EP2120915B1 (de) 2011-09-28
RU2474585C2 (ru) 2013-02-10
US7947813B2 (en) 2011-05-24
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CA2673851C (en) 2016-05-03
KR101431856B1 (ko) 2014-08-27
KR20090113264A (ko) 2009-10-29
EP2377527A1 (de) 2011-10-19
WO2008091740A4 (en) 2009-01-08
EP2120915A2 (de) 2009-11-25
CA2673851A1 (en) 2008-07-31
US20080193981A1 (en) 2008-08-14
WO2008091740A3 (en) 2008-11-20
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CN101646431A (zh) 2010-02-10

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