ATE526017T1 - Abscheidung von polyelektrolyten und reinigung von antikörpern - Google Patents

Abscheidung von polyelektrolyten und reinigung von antikörpern

Info

Publication number
ATE526017T1
ATE526017T1 AT08705841T AT08705841T ATE526017T1 AT E526017 T1 ATE526017 T1 AT E526017T1 AT 08705841 T AT08705841 T AT 08705841T AT 08705841 T AT08705841 T AT 08705841T AT E526017 T1 ATE526017 T1 AT E526017T1
Authority
AT
Austria
Prior art keywords
polyelectrolytes
antibodies
purification
deposition
polyelectrolyte
Prior art date
Application number
AT08705841T
Other languages
English (en)
Inventor
Robert Fahrner
Jayme Franklin
Paul Mcdonald
Thanmaya Peram
Vikram Sisodiya
Corazon Victa
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of ATE526017T1 publication Critical patent/ATE526017T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
    • A61K31/197—Carboxylic acids, e.g. valproic acid having an amino group the amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine, epsilon-aminocaproic acid, pantothenic acid
    • A61K31/198—Alpha-aminoacids, e.g. alanine, edetic acids [EDTA]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/32—Extraction; Separation; Purification by precipitation as complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2878—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2887—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
    • C07K2317/734—Complement-dependent cytotoxicity [CDC]
AT08705841T 2007-01-22 2008-01-10 Abscheidung von polyelektrolyten und reinigung von antikörpern ATE526017T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US88606807P 2007-01-22 2007-01-22
US1344607P 2007-12-13 2007-12-13
PCT/US2008/050747 WO2008091740A2 (en) 2007-01-22 2008-01-10 Polyelectrolyte precipitation and purification of antibodies

Publications (1)

Publication Number Publication Date
ATE526017T1 true ATE526017T1 (de) 2011-10-15

Family

ID=39247681

Family Applications (1)

Application Number Title Priority Date Filing Date
AT08705841T ATE526017T1 (de) 2007-01-22 2008-01-10 Abscheidung von polyelektrolyten und reinigung von antikörpern

Country Status (17)

Country Link
US (1) US7947813B2 (de)
EP (2) EP2377527B1 (de)
JP (1) JP5361738B2 (de)
KR (1) KR101431856B1 (de)
CN (1) CN101646431B (de)
AT (1) ATE526017T1 (de)
AU (1) AU2008209404B2 (de)
BR (1) BRPI0806367A2 (de)
CA (1) CA2673851C (de)
ES (2) ES2374330T3 (de)
HK (1) HK1135920A1 (de)
IL (1) IL199550A (de)
MX (1) MX2009007632A (de)
NZ (1) NZ577933A (de)
RU (1) RU2474585C2 (de)
WO (1) WO2008091740A2 (de)
ZA (1) ZA200904482B (de)

Families Citing this family (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7132100B2 (en) 2002-06-14 2006-11-07 Medimmune, Inc. Stabilized liquid anti-RSV antibody formulations
FR2902799B1 (fr) 2006-06-27 2012-10-26 Millipore Corp Procede et unite de preparation d'un echantillon pour l'analyse microbiologique d'un liquide
US9375499B2 (en) * 2006-07-14 2016-06-28 Wisconsin Alumni Research Foundation Adsorptive membranes for trapping viruses
US8362217B2 (en) 2006-12-21 2013-01-29 Emd Millipore Corporation Purification of proteins
US8569464B2 (en) 2006-12-21 2013-10-29 Emd Millipore Corporation Purification of proteins
US8163886B2 (en) 2006-12-21 2012-04-24 Emd Millipore Corporation Purification of proteins
WO2009151514A1 (en) 2008-06-11 2009-12-17 Millipore Corporation Stirred tank bioreactor
US8592555B2 (en) 2008-08-11 2013-11-26 Emd Millipore Corporation Immunoglobulin-binding proteins with improved specificity
WO2010019493A1 (en) * 2008-08-14 2010-02-18 Merck Sharp & Dohme Corp. Methods for purifying antibodies using protein a affinity chromatography
US8846877B2 (en) * 2008-09-25 2014-09-30 Jsr Corporation Packing material for affinity chromatography
GB0818228D0 (en) * 2008-10-06 2008-11-12 Avecia Biolog Ltd Purification process
WO2010074953A1 (en) 2008-12-16 2010-07-01 Millipore Corporation Stirred tank reactor and method
BRPI0923541A2 (pt) 2008-12-22 2016-01-26 Hoffmann La Roche purificação de imunoglobulina
SG162687A1 (en) 2008-12-24 2010-07-29 Millipore Corp Caustic stable chromatography ligands
US20120022239A1 (en) * 2009-01-13 2012-01-26 Ge Healthcare Bio-Sciences Ab Precipitation of biomolecules with negatively charged polymers
EP3736338A1 (de) * 2009-09-01 2020-11-11 F. Hoffmann-La Roche AG Verbesserte proteinreinigung durch eine modifizierte protein-a-elution
CN102666568B (zh) 2009-12-18 2015-12-09 杰特有限公司 纯化多肽的方法
US8377672B2 (en) 2010-02-18 2013-02-19 3M Innovative Properties Company Ligand functionalized polymers
WO2011109151A1 (en) 2010-03-03 2011-09-09 3M Innovative Properties Company Ligand guanidinyl functionalized polymers
US8691918B2 (en) * 2010-05-17 2014-04-08 Emd Millipore Corporation Stimulus responsive polymers for the purification of biomolecules
CA2814781A1 (en) * 2010-11-01 2012-05-10 Diderik Reinder Kremer Single unit ion exchange chromatography antibody purification
CN103547589A (zh) * 2011-03-29 2014-01-29 葛兰素史密斯克莱有限责任公司 用于蛋白纯化的缓冲液体系
IL212911A0 (en) * 2011-05-16 2011-07-31 Omrix Biopharmaceuticals Ltd Immunoglobulin reduced in thrombogenic contaminants and preparation thereof
SG10201604554WA (en) 2011-06-08 2016-07-28 Emd Millipore Corp Chromatography matrices including novel staphylococcus aureus protein a based ligands
AU2012290004B2 (en) * 2011-08-02 2014-11-27 Takeda Pharmaceutical Company Limited Systems and methods to increase protein yield from recombinant manufacturing processes
EP2744518A2 (de) * 2011-08-19 2014-06-25 EMD Millipore Corporation Verwendung kleiner moleküle in verfahren zur reinigung von biomolekülen
EP2578286A1 (de) 2011-10-04 2013-04-10 Merck Patent GmbH Verfahren und Vorrichtung zur chromatographischen Aufreinigung
EP2656892A1 (de) 2012-04-23 2013-10-30 Merck Patent GmbH Chromatographieverfahren
JP6297029B2 (ja) 2012-05-31 2018-03-20 エイジェンシー・フォー・サイエンス，テクノロジー・アンド・リサーチ 多様な官能基を有する粒子による免疫グロブリンｇ調製物のクロマトグラフィー精製
JP6268167B2 (ja) 2012-05-31 2018-01-24 エイジェンシー フォー サイエンス，テクノロジー アンド リサーチ タンパク質製剤中の凝集物含量を低減するために混合型多官能表面を使用する方法
KR102067445B1 (ko) 2012-05-31 2020-01-17 에이전시 포 사이언스, 테크놀로지 앤드 리서치 양전하 유기 첨가물 처리에 의한 단백질-오염물 복합체 및 단백질 제제 내 응집체 레벨의 감소 방법
WO2014003137A1 (ja) * 2012-06-27 2014-01-03 旭化成メディカル株式会社 高アフィニティー抗体、及びその製造方法
EP2682168A1 (de) 2012-07-02 2014-01-08 Millipore Corporation Reinigung biologischer Moleküle
LT2890710T (lt) * 2012-08-28 2017-10-25 Novartis Ag Antikūnų gryninimo būdai, panaudojant alifatinius alkoholius
KR20150084836A (ko) * 2012-11-05 2015-07-22 메디뮨 엘엘씨 벤조나아제 부재 하의 시나지스 분리 방법
WO2014094957A1 (en) 2012-12-20 2014-06-26 Merck Patent Gmbh Copolymers for protein precipitation
US9975919B2 (en) 2013-02-06 2018-05-22 Agency For Science, Technology And Research Methods for reducing aggregate content in protein preparations
WO2014133458A1 (en) * 2013-02-26 2014-09-04 Agency For Science, Technology And Research Protein purification in the presence of nonionic organic polymers and electropositive surfaces
US9850512B2 (en) 2013-03-15 2017-12-26 The Research Foundation For The State University Of New York Hydrolysis of cellulosic fines in primary clarified sludge of paper mills and the addition of a surfactant to increase the yield
WO2015007638A1 (en) 2013-07-18 2015-01-22 Chr. Hansen A/S Milk clotting aspartic protease enzyme composition
CA2919288A1 (en) 2013-08-23 2015-02-26 Boehringer Ingelheim Rcv Gmbh & Co Kg Microparticles for cell disruption and/or biomolecule recovery
MX2016002798A (es) 2013-09-05 2016-07-21 Genentech Inc Metodo para reutilizacion de cromatografia.
SG11201602266YA (en) * 2013-09-24 2016-04-28 Elastagen Pty Ltd Method of extracting protein
GB201318840D0 (en) * 2013-10-24 2013-12-11 Univ Leeds Method and device for protein preparation
WO2015075070A1 (en) * 2013-11-20 2015-05-28 Cmc Biologics A/S A bioreactor system and method for producing a biopolymer
ES2864721T3 (es) 2014-02-07 2021-10-14 Cmc Biologics As Disposición y proceso continuo de un biorreactor para la producción y captura de un biopolímero
US9951363B2 (en) 2014-03-14 2018-04-24 The Research Foundation for the State University of New York College of Environmental Science and Forestry Enzymatic hydrolysis of old corrugated cardboard (OCC) fines from recycled linerboard mill waste rejects
AU2015248888A1 (en) 2014-04-16 2016-10-20 Cmc Biologics A/S A high cell density fill and draw fermentation process
KR101891455B1 (ko) 2014-11-03 2018-08-27 메르크 파텐트 게엠베하 생체분자를 정제하기 위한 가용성 인테인 융합 단백질 및 방법
EP3265557B1 (de) * 2015-03-06 2019-10-16 F. Hoffmann-La Roche AG Ultrareine dsba und dsbc und verfahren zur herstellung und verwendung davon
WO2017022651A1 (ja) 2015-07-31 2017-02-09 中外製薬株式会社 アニオン性ポリマーによる抗体を含有する組成物の精製方法
JP7349998B2 (ja) * 2018-01-31 2023-09-25 リジェネロン・ファーマシューティカルズ・インコーポレイテッド サイズバリアントおよび電荷バリアントである薬物製品不純物を特徴解析するためのシステムおよび方法
EP3816177A4 (de) * 2018-06-29 2021-12-01 Mitsubishi Chemical Corporation Verfahren zur polypeptidisolierung, verfahren zur polypeptidherstellung und vorrichtung zur polypeptidreinigung
JP6564539B1 (ja) 2018-09-14 2019-08-21 長瀬産業株式会社 スルホン酸化合物によるペプチド精製方法
HUP1900112A1 (hu) 2019-04-04 2020-10-28 Richter Gedeon Nyrt Immunglobulinok affinitás kromatográfiájának fejlesztése kötést megelõzõ flokkulálás alkalmazásával
AR119264A1 (es) * 2019-06-05 2021-12-09 Genentech Inc Método para reutilización de cromatografía
KR102612555B1 (ko) * 2020-12-18 2023-12-11 한국세라믹기술원 항체결합단백질 및 칼시퀘스트린 융합단백질을 이용한 면역침강 방법
WO2023068999A1 (en) * 2021-10-21 2023-04-27 Nyctea Technology Ab Device for separating an analyte from other components in an electrolytic solution
CN114574457A (zh) * 2022-01-25 2022-06-03 华东理工大学 一种快速分离纯化蛋白质的方法

Family Cites Families (92)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3252961A (en) * 1961-04-03 1966-05-24 Foremost Dairies Inc Whey process and product
US3737377A (en) * 1971-01-27 1973-06-05 Miles Lab Purification of lactase
US4055469A (en) * 1976-12-10 1977-10-25 Eastman Kodak Company Purification of microbial enzyme extracts using synthetic polyelectrolytes
USRE30985E (en) 1978-01-01 1982-06-29 Serum-free cell culture media
GB2036891B (en) 1978-12-05 1983-05-05 Windsor Smith C Change speed gear
US4515893A (en) 1979-04-26 1985-05-07 Ortho Pharmaceutical Corporation Hybrid cell line for producing complement-fixing monoclonal antibody to human T cells
CA1213229A (en) 1982-04-12 1986-10-28 Gary S. David Antibodies having dual specificities, their preparation and uses therefor
US4560655A (en) 1982-12-16 1985-12-24 Immunex Corporation Serum-free cell culture medium and process for making same
US4657866A (en) 1982-12-21 1987-04-14 Sudhir Kumar Serum-free, synthetic, completely chemically defined tissue culture media
GB8308235D0 (en) 1983-03-25 1983-05-05 Celltech Ltd Polypeptides
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
DD266710A3 (de) 1983-06-06 1989-04-12 Ve Forschungszentrum Biotechnologie Verfahren zur biotechnischen Herstellung van alkalischer Phosphatase
US4767704A (en) 1983-10-07 1988-08-30 Columbia University In The City Of New York Protein-free culture medium
US4533496A (en) * 1984-05-08 1985-08-06 Monsanto Company Method of isolating monoclonal antibodies from hybridoma cultures
US5672347A (en) 1984-07-05 1997-09-30 Genentech, Inc. Tumor necrosis factor antagonists and their use
JPS6147500A (ja) 1984-08-15 1986-03-07 Res Dev Corp Of Japan キメラモノクロ−ナル抗体及びその製造法
EP0173494A3 (de) 1984-08-27 1987-11-25 The Board Of Trustees Of The Leland Stanford Junior University Chimäre Rezeptoren durch Verbindung und Expression von DNS
GB8422238D0 (en) 1984-09-03 1984-10-10 Neuberger M S Chimeric proteins
US4879231A (en) 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
JPS61134325A (ja) 1984-12-04 1986-06-21 Teijin Ltd ハイブリツド抗体遺伝子の発現方法
GB8516415D0 (en) 1985-06-28 1985-07-31 Celltech Ltd Culture of animal cells
CA1282069C (en) 1985-09-12 1991-03-26 Damon L. Meyer Antibody complexes of hapten-modified diagnostic or therapeutic agents
DE3689123T2 (de) 1985-11-01 1994-03-03 Xoma Corp Modulare einheit von antikörpergenen, daraus hergestellte antikörper und verwendung.
US5225539A (en) 1986-03-27 1993-07-06 Medical Research Council Recombinant altered antibodies and methods of making altered antibodies
US4927762A (en) 1986-04-01 1990-05-22 Cell Enterprises, Inc. Cell culture medium with antioxidant
GB8610600D0 (en) 1986-04-30 1986-06-04 Novo Industri As Transformation of trichoderma
US4946778A (en) 1987-09-21 1990-08-07 Genex Corporation Single polypeptide chain binding molecules
IL85035A0 (en) 1987-01-08 1988-06-30 Int Genetic Eng Polynucleotide molecule,a chimeric antibody with specificity for human b cell surface antigen,a process for the preparation and methods utilizing the same
US5091313A (en) 1988-08-05 1992-02-25 Tanox Biosystems, Inc. Antigenic epitopes of IgE present on B cell but not basophil surface
US5720937A (en) 1988-01-12 1998-02-24 Genentech, Inc. In vivo tumor detection assay
JP2749167B2 (ja) 1988-06-21 1998-05-13 ジェネンテク，インコーポレイテッド 心筋梗塞の処置のための方法および治療用組成物
EP0435911B1 (de) 1988-09-23 1996-03-13 Cetus Oncology Corporation Zellenzuchtmedium für erhöhtes zellenwachstum, zur erhöhung der langlebigkeit und expression der produkte
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
EP0402226A1 (de) 1989-06-06 1990-12-12 Institut National De La Recherche Agronomique Transformationsvektoren für Hefe Yarrowia
US5661016A (en) 1990-08-29 1997-08-26 Genpharm International Inc. Transgenic non-human animals capable of producing heterologous antibodies of various isotypes
ES2246502T3 (es) 1990-08-29 2006-02-16 Genpharm International, Inc. Animales no humanos transgenicos capaces de producir anticuerpos heterologos.
US5633425A (en) 1990-08-29 1997-05-27 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5625126A (en) 1990-08-29 1997-04-29 Genpharm International, Inc. Transgenic non-human animals for producing heterologous antibodies
US5545806A (en) 1990-08-29 1996-08-13 Genpharm International, Inc. Ransgenic non-human animals for producing heterologous antibodies
US5122469A (en) 1990-10-03 1992-06-16 Genentech, Inc. Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins
ES2296839T3 (es) 1991-08-14 2008-05-01 Genentech Inc. Variantes de inmunoglobulinas para receptores fc-epsilon especificos.
WO1993008829A1 (en) 1991-11-04 1993-05-13 The Regents Of The University Of California Compositions that mediate killing of hiv-infected cells
US5525519A (en) * 1992-01-07 1996-06-11 Middlesex Sciences, Inc. Method for isolating biomolecules from a biological sample with linear polymers
ATE149570T1 (de) 1992-08-17 1997-03-15 Genentech Inc Bispezifische immunoadhesine
DE69315847T2 (de) 1992-08-21 1998-06-25 Genentech Inc Verfahren zur behandlung einer durch lfa-1 vermittelten störung
US5736137A (en) 1992-11-13 1998-04-07 Idec Pharmaceuticals Corporation Therapeutic application of chimeric and radiolabeled antibodies to human B lymphocyte restricted differentiation antigen for treatment of B cell lymphoma
US5595721A (en) 1993-09-16 1997-01-21 Coulter Pharmaceutical, Inc. Radioimmunotherapy of lymphoma using anti-CD20
CA2176811C (en) 1993-12-10 2008-01-29 David Tai Wai Fei Methods for diagnosis of allergy and screening of anti-allergy therapeutics
AP660A (en) 1994-01-18 1998-08-18 Genentech Inc A method of treatment of parasitic infection using IgE antagonists.
US5702946A (en) 1994-03-03 1997-12-30 Genentech, Inc. Anti-IL-8 monoclonal antibodies for treatment of inflammatory disorders
US5922531A (en) * 1994-06-21 1999-07-13 Advanced Research And Technology Polyelectrolyte treated glass for enzyme immobilization and protein purification
DE69534530T2 (de) 1994-08-12 2006-07-06 Immunomedics, Inc. Für b-zell-lymphom und leukämiezellen spezifische immunkonjugate und humane antikörper
DE69535433T2 (de) * 1994-09-21 2007-06-28 Kanegafuchi Kagaku Kogyo K.K. Verwendung eines Adsorptionsmittels für Interleukine, Verfahren zu deren Entfernung
IL117645A (en) 1995-03-30 2005-08-31 Genentech Inc Vascular endothelial cell growth factor antagonists for use as medicaments in the treatment of age-related macular degeneration
US5641870A (en) * 1995-04-20 1997-06-24 Genentech, Inc. Low pH hydrophobic interaction chromatography for antibody purification
JPH11507535A (ja) 1995-06-07 1999-07-06 イムクローン システムズ インコーポレイテッド 腫瘍の成長を抑制する抗体および抗体フラグメント類
EP1297847A3 (de) 1996-01-23 2003-05-07 Genentech, Inc. Antikörper gegen CD18 bei Gehirnschlag
US7147851B1 (en) 1996-08-15 2006-12-12 Millennium Pharmaceuticals, Inc. Humanized immunoglobulin reactive with α4β7 integrin
SI1897890T1 (sl) 1996-11-27 2010-03-31 Genentech Inc Afinitetno čiščenje polipeptida na proteinskemA matriksu
JP3778945B2 (ja) 1996-11-27 2006-05-24 ジェネンテック・インコーポレーテッド ヒト化抗ＣＤ１１ａ抗体
US6872568B1 (en) 1997-03-17 2005-03-29 Human Genome Sciences, Inc. Death domain containing receptor 5 antibodies
ES2236634T3 (es) 1997-04-07 2005-07-16 Genentech, Inc. Anticuerpos anti-vegf.
JP2001511653A (ja) 1997-05-15 2001-08-14 ジェネンテク，インコーポレイテッド Ａｐｏ−２レセプター
US6265542B1 (en) * 1997-10-24 2001-07-24 Genentech, Inc. Purification of molecules
US6087448A (en) 1997-11-19 2000-07-11 Amcol International Corporation Solid superabsorbent material containing a poly(vinylguanidine) and an acidic water-absorbing resin
PT1308456E (pt) * 1998-05-06 2007-12-03 Genentech Inc Purificação de anticorpos por cromatografia de permuta iónica
US6217926B1 (en) * 1998-06-30 2001-04-17 Michael Foods, Inc. Aqueous extraction process to selectively remove phospholipid from egg yolks
US6307013B1 (en) * 1998-07-21 2001-10-23 Monsanto Technology Llc Clarification of protein precipitate suspensions using anionic polymeric flocculants
US6946129B1 (en) 1999-06-08 2005-09-20 Seattle Genetics, Inc. Recombinant anti-CD40 antibody and uses thereof
ATE400587T1 (de) 1999-10-29 2008-07-15 Genentech Inc Gegen das prostata-stammzellantigen (psca) gerichtete antikörper und deren verwendung
US20030133939A1 (en) 2001-01-17 2003-07-17 Genecraft, Inc. Binding domain-immunoglobulin fusion proteins
US7321026B2 (en) 2001-06-27 2008-01-22 Skytech Technology Limited Framework-patched immunoglobulins
JP4338523B2 (ja) * 2001-11-16 2009-10-07 ノルゲン バイオテク コーポレイション 蛋白質の精製方法
US20040093621A1 (en) 2001-12-25 2004-05-13 Kyowa Hakko Kogyo Co., Ltd Antibody composition which specifically binds to CD20
ATE417858T1 (de) * 2002-02-05 2009-01-15 Genentech Inc Proteinaufreinigung
CN101914158A (zh) 2002-02-14 2010-12-15 免疫医疗公司 抗cd 20抗体及其融合蛋白和使用方法
WO2004014942A1 (en) 2002-08-09 2004-02-19 Selborne Biological Services (Australia) Pty Limited A method for preparing lipoprotein from a blood source
KR20100050587A (ko) 2002-10-17 2010-05-13 젠맵 에이/에스 Ｃｄ20에 대한 인간 모노클로날 항체
JP4351674B2 (ja) 2002-12-16 2009-10-28 ジェネンテック・インコーポレーテッド 免疫グロブリン変異体とその使用法およびその使用
US20040121339A1 (en) * 2002-12-19 2004-06-24 Jizhong Zhou Special film-coated substrate for bio-microarray fabrication and use thereof
WO2004092393A1 (en) * 2003-01-09 2004-10-28 Genentech, Inc. Purification of polypeptides
AU2004252067B2 (en) 2003-05-09 2012-04-12 Duke University CD20-specific antibodies and methods of employing same
AR044388A1 (es) 2003-05-20 2005-09-07 Applied Molecular Evolution Moleculas de union a cd20
WO2005016968A2 (en) * 2003-07-28 2005-02-24 Genentech, Inc. Reducing protein a leaching during protein a affinity chromatography
EP1651663B1 (de) 2003-08-08 2017-05-17 Immunomedics, Inc. Bispezifische antikörper zur induktion der apoptose in tumorzellen und erkrankten zellen
US8147832B2 (en) 2003-08-14 2012-04-03 Merck Patent Gmbh CD20-binding polypeptide compositions and methods
WO2005044859A2 (en) 2003-11-05 2005-05-19 Glycart Biotechnology Ag Cd20 antibodies with increased fc receptor binding affinity and effector function
US20050147581A1 (en) * 2003-11-19 2005-07-07 The Board Of Trustees Of The University Of Illinois Macromolecular drug complexes having improved stability and therapeutic use of the same
UA86605C2 (ru) 2004-01-12 2009-05-12 Аплайд Молекьюлер Иволюшн, Инк. Антитело, которое содержит вариант исходного человеческого fс-участка
EP1740946B1 (de) 2004-04-20 2013-11-06 Genmab A/S Humane monoklonale antikörper gegen cd20
US7673757B2 (en) 2006-02-17 2010-03-09 Millipore Corporation Adsorbent filter media for removal of biological contaminants in process liquids
US8163886B2 (en) * 2006-12-21 2012-04-24 Emd Millipore Corporation Purification of proteins

Also Published As

Publication number Publication date
ES2426158T3 (es) 2013-10-21
JP2010516773A (ja) 2010-05-20
EP2377527B1 (de) 2013-07-17
WO2008091740A2 (en) 2008-07-31
US7947813B2 (en) 2011-05-24
EP2377527A1 (de) 2011-10-19
AU2008209404B2 (en) 2012-08-16
CN101646431B (zh) 2012-06-27
EP2120915B1 (de) 2011-09-28
MX2009007632A (es) 2009-07-24
US20080193981A1 (en) 2008-08-14
AU2008209404A1 (en) 2008-07-31
ES2374330T3 (es) 2012-02-15
KR101431856B1 (ko) 2014-08-27
CN101646431A (zh) 2010-02-10
CA2673851C (en) 2016-05-03
BRPI0806367A2 (pt) 2011-09-06
KR20090113264A (ko) 2009-10-29
RU2009131765A (ru) 2011-02-27
NZ577933A (en) 2011-12-22
RU2474585C2 (ru) 2013-02-10
WO2008091740A3 (en) 2008-11-20
EP2120915A2 (de) 2009-11-25
JP5361738B2 (ja) 2013-12-04
ZA200904482B (en) 2010-09-29
IL199550A (en) 2015-04-30
WO2008091740A4 (en) 2009-01-08
CA2673851A1 (en) 2008-07-31
HK1135920A1 (en) 2010-06-18

Similar Documents

Publication Publication Date Title
ATE526017T1 (de) Abscheidung von polyelektrolyten und reinigung von antikörpern
EA201500531A1 (ru) Регулирование состава газа в газосепарационной установке с мембранами
EA201070987A1 (ru) Очищенные гибридные белки иммуноглобулина и способы их очищения
EA201270557A1 (ru) Способ очистки аденовируса из культур с высокой плотностью клеток
GB201020995D0 (en) Biological materials and uses thereof
FI20115722A0 (fi) Menetelmä ja laite biologista alkuperää olevan materiaalin puhdistamiseksi
PH12020500027A1 (en) Purification of iduronate-2-sulfatase
AR057731A1 (es) Metodo y sistema para purificar un gas
WO2011136624A3 (ko) 자기장 인가부를 구비한 생물학적 시료 자동정제장치, 생물학적 시료로부터 타겟물질을 추출하는 방법, 그리고 단백질 발현 및 정제 방법
WO2010120616A3 (en) Process for the purification of paraxylene
ATE512120T1 (de) Anaerobe reinigung von abwasser
MX344970B (es) Medio de cultivo celular para la expresion de la proteina adamts.
EA201390639A1 (ru) Производные 2-пиперазин-1-ил-4н-1,3-бензотиазин-4-она и их применение для лечения инфекций у млекопитающих
WO2008085962A3 (en) Cell culture methods for producing recombinant proteins in the presence of reduced levels of one or more contaminants
BR112012015117A2 (pt) "biorreator de membrana para produção aumentada de gás isopreno"
UA108648C2 (uk) Спосіб обробки рослинної олії
ATE527248T1 (de) Polymorphe von enantioreinem erdostein
RU112188U8 (ru) Установка очистки сульфидно-щелочных стоков
GB2470744B (en) Novel protein crystallization technique
IL199752A0 (en) Purification of taci-fc
EA201270403A1 (ru) Способ и система для очистки полипептидов
RU2011131558A (ru) Способ очистки бактериоцинов
TR2023014436T (tr) Afi̇ni̇te kromatografi̇si̇ i̇le protei̇n saflaştirilmasi
WO2011028623A3 (en) Modified organisms for improved saccharification of biomass
RU2012143225A (ru) Способ выделения и очистки иммобилизованного фермента

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties