EA201270557A1 - Способ очистки аденовируса из культур с высокой плотностью клеток - Google Patents

Способ очистки аденовируса из культур с высокой плотностью клеток

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Publication number
EA201270557A1
EA201270557A1 EA201270557A EA201270557A EA201270557A1 EA 201270557 A1 EA201270557 A1 EA 201270557A1 EA 201270557 A EA201270557 A EA 201270557A EA 201270557 A EA201270557 A EA 201270557A EA 201270557 A1 EA201270557 A1 EA 201270557A1
Authority
EA
Eurasian Patent Office
Prior art keywords
adenovirus
high density
cultures
cleaning
density cells
Prior art date
Application number
EA201270557A
Other languages
English (en)
Other versions
EA028875B1 (ru
Inventor
Марсел Лео Де Вохт
Марлус Венстра
Original Assignee
Круселл Холланд Б.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Круселл Холланд Б.В. filed Critical Круселл Холланд Б.В.
Publication of EA201270557A1 publication Critical patent/EA201270557A1/ru
Publication of EA028875B1 publication Critical patent/EA028875B1/ru

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10351—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Изобретение относится к способам крупномасштабной очистки аденовируса из суспензий с высокой плотностью клеток с применением фрагментации и/или осаждения ДНК с последующей стадией осветления фильтрацией в тангенциальном потоке.
EA201270557A 2009-10-15 2010-10-14 Способ очистки аденовирусных частиц из клеточной суспензии EA028875B1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US27906009P 2009-10-15 2009-10-15
EP09173119 2009-10-15
PCT/EP2010/065436 WO2011045381A1 (en) 2009-10-15 2010-10-14 Process for adenovirus purification from high cell density cultures

Publications (2)

Publication Number Publication Date
EA201270557A1 true EA201270557A1 (ru) 2012-09-28
EA028875B1 EA028875B1 (ru) 2018-01-31

Family

ID=41563777

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201270557A EA028875B1 (ru) 2009-10-15 2010-10-14 Способ очистки аденовирусных частиц из клеточной суспензии

Country Status (16)

Country Link
US (1) US8470585B2 (ru)
EP (1) EP2488635B1 (ru)
JP (1) JP5465331B2 (ru)
KR (1) KR101805938B1 (ru)
CN (1) CN102575233B (ru)
AU (1) AU2010305768B2 (ru)
BR (1) BR112012008507B8 (ru)
CA (1) CA2777116C (ru)
DK (1) DK2488635T3 (ru)
EA (1) EA028875B1 (ru)
ES (1) ES2445713T3 (ru)
HK (1) HK1174949A1 (ru)
MX (1) MX2012004221A (ru)
PL (1) PL2488635T3 (ru)
WO (1) WO2011045381A1 (ru)
ZA (1) ZA201202524B (ru)

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Also Published As

Publication number Publication date
BR112012008507B1 (pt) 2021-05-04
CN102575233B (zh) 2014-07-16
HK1174949A1 (en) 2013-06-21
WO2011045381A1 (en) 2011-04-21
PL2488635T3 (pl) 2014-04-30
JP2013507141A (ja) 2013-03-04
DK2488635T3 (en) 2014-02-24
AU2010305768A1 (en) 2012-04-12
CA2777116C (en) 2020-09-01
JP5465331B2 (ja) 2014-04-09
EA028875B1 (ru) 2018-01-31
AU2010305768B2 (en) 2015-05-14
MX2012004221A (es) 2012-06-08
BR112012008507B8 (pt) 2021-05-25
ES2445713T3 (es) 2014-03-04
US20120202268A1 (en) 2012-08-09
CN102575233A (zh) 2012-07-11
US8470585B2 (en) 2013-06-25
BR112012008507A2 (pt) 2015-09-15
KR20120093957A (ko) 2012-08-23
CA2777116A1 (en) 2011-04-21
EP2488635B1 (en) 2013-11-20
KR101805938B1 (ko) 2018-01-10
EP2488635A1 (en) 2012-08-22
ZA201202524B (en) 2012-12-27

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